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Author Husted, L.; Andersen, M.S.; Borggaard, O.K.; Houe, H.; Olsen, S.N.
Title Risk factors for faecal sand excretion in Icelandic horses Type Journal Article
Year 2005 Publication Equine Veterinary Journal Abbreviated Journal Equine Vet J
Volume 37 Issue 4 Pages 351-355
Keywords Animal Feed; Animal Husbandry/methods; Animals; Denmark; Feces/*chemistry; Female; Gastrointestinal Diseases/epidemiology/etiology/prevention & control/veterinary; Horse Diseases/epidemiology/etiology/prevention & control; Horses/*metabolism; Logistic Models; Male; Pilot Projects; *Poaceae/growth & development; Risk Factors; Silicon Dioxide/administration & dosage/adverse effects/*analysis; Soil/*analysis
Abstract REASONS FOR PERFORMING STUDY: Sandy soil is often mentioned as a risk factor in the development of sand-related gastrointestinal disease (SGID) in the horse. There are other variables, but few studies confirm any of these. OBJECTIVE: To investigate soil type, pasture quality, feeding practice in the paddock, age, sex and body condition score as risk factors for sand intake in the horse. METHODS: Faeces were collected from 211 Icelandic horses on 19 different studs in Denmark together with soil samples and other potential risk factors. Sand content in faeces determined by a sand sedimentation test was interpreted as evidence of sand intake. Soil types were identified by soil analysis and significance of the data was tested using logistic analysis. RESULTS: Of horses included in the study, 56.4% showed sand in the faeces and 5.7% had more than 5 mm sand as quantified by the rectal sleeve sedimentation test. Soil type had no significant effect when tested as main effect, but there was interaction between soil type and pasture quality. Significant interactions were also found between paddock feeding practice and pasture quality. CONCLUSION: To evaluate the risk of sand intake it is important to consider 3 variables: soil type, pasture quality and feeding practice. Pasture quality was identified as a risk factor of both short and long grass in combination with sandy soil, while clay soil had the lowest risk in these combinations. Feeding practice in the paddock revealed feeding directly on the ground to be a risk factor when there was short (1-5 cm) or no grass. Also, no feeding outdoors increased the risk on pastures with short grass, while this had no effect in paddocks with no grass. More than 50% of all horses investigated in this study had sand in the faeces. POTENTIAL RELEVANCE: The identification of risk factors is an important step towards prevention of SGID. Further research is necessary to determine why some horses exhibit more than 5 mm sand in the sedimentation test and whether this is correlated with geophagic behaviour.
Address Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlaegevej 88, DK-1870 Frederiksberg, Denmark
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0425-1644 ISBN Medium
Area Expedition Conference
Notes PMID:16028626 Approved no
Call Number (up) Serial 1888
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Author Lucas, Z.; Raeside, J.I.; Betteridge, K.J.
Title Non-invasive assessment of the incidences of pregnancy and pregnancy loss in the feral horses of Sable Island Type Journal Article
Year 1991 Publication Journal of Reproduction and Fertility. Supplement Abbreviated Journal J Reprod Fertil Suppl
Volume 44 Issue Pages 479-488
Keywords Abortion, Veterinary/*epidemiology; Animals; Animals, Wild/*physiology; Atlantic Islands; Estrogens/analysis; Feces/chemistry; Female; Fertilization; Gestational Age; Horse Diseases/*epidemiology; Horses; Incidence; Pregnancy; *Pregnancy, Animal
Abstract Field observations of 400 totally unmanaged feral horses on Sable Island, Nova Scotia, were complemented by oestrogen determinations in faecal samples from 154 identified females over a 4-year period (454 mare-years). Of mares that were sampled throughout the year and subsequently produced foals, 92.1% exhibited elevated faecal oestrogens between 15 October and 30 March. The results confirm that faecal oestrogens are a useful indicator of pregnancy after approximately 120 days gestation. Distribution of foaling resembled that seen in other feral populations, with 95% of births occurring from April through July. The foaling rate for mares aged 3 years or older was 62.0%, with 50.7% of mares foaling in 3 or 4 years. Foaling rates were low (4.1%) in mares bred as yearlings and rose with age to 70.8% in those bred as 4-year-olds. Fetal loss after Day 120 was deduced from faecal oestrogens to be 26.0% overall, with marked variation from year to year (9.6-37.3%) and with age (70.0% in those bred as yearlings, decreasing to 5.6% in those bred as 4-year-olds). Of 58 mares aged 2 years or older that were sampled every year, about half (49.6%) the barren years were attributable to fetal loss after 120 days gestation. All mares conceived in at least 2 of the 4 years, suggesting that pregnancy loss, even after Day 120, is as important as failure to conceive in causing barren years.
Address Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0449-3087 ISBN Medium
Area Expedition Conference
Notes PMID:1795292 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2247
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Author Milinovich, G.J.; Trott, D.J.; Burrell, P.C.; van Eps, A.W.; Thoefner, M.B.; Blackall, L.L.; Al Jassim, R.A.M.; Morton, J.M.; Pollitt, C.C.
Title Changes in equine hindgut bacterial populations during oligofructose-induced laminitis Type Journal Article
Year 2006 Publication Environmental Microbiology Abbreviated Journal Environ Microbiol
Volume 8 Issue 5 Pages 885-898
Keywords Animal Feed; Animals; Bacteria/classification/*isolation & purification; DNA, Bacterial/analysis; Disease Models, Animal; Feces/microbiology; Foot Diseases/etiology/microbiology/*veterinary; Horse Diseases/*etiology/metabolism/microbiology; Horses; In Situ Hybridization, Fluorescence; Intestines/*microbiology; Oligosaccharides/*administration & dosage/*metabolism; Phylogeny; Polymerase Chain Reaction; RNA, Bacterial/analysis; RNA, Ribosomal, 16S/analysis
Abstract In the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. The objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. Laminitis was induced in five horses by administration of oligofructose. Faecal specimens were collected at 8 h intervals from 72 h before to 72 h after the administration of oligofructose. Hindgut microbiota able to utilize oligofructose were enumerated throughout the course of the experiment using habitat-simulating medium. Isolates were collected and representatives identified by 16S rRNA gene sequencing. The majority of these isolates collected belonged to the genus Streptococcus, 91% of which were identified as being most closely related to Streptococcus infantarius ssp. coli. Furthermore, S. infantarius ssp. coli was the predominant oligofructose-utilizing organism isolated before the onset of lameness. Fluorescence in situ hybridization probes developed to specifically target the isolated Streptococcus spp. demonstrated marked population increases between 8 and 16 h post oligofructose administration. This was followed by a rapid population decline which corresponded with a sharp decline in faecal pH and subsequently lameness at 24-32 h post oligofructose administration. This research suggests that streptococci within the Streptococcus bovis/equinus complex may be involved in the series of events which precede the onset of laminitis in the horse.
Address Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Queensland, Australia. g.milinovich@uq.edu.au
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1462-2912 ISBN Medium
Area Expedition Conference
Notes PMID:16623745 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2625
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Author Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B.
Title Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces Type Journal Article
Year 2004 Publication Parasitology Abbreviated Journal Parasitology
Volume 129 Issue Pt 6 Pages 733-739
Keywords Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics
Abstract Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1820 ISBN Medium
Area Expedition Conference
Notes PMID:15648696 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2631
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Author Traversa, D.; Giangaspero, A.; Galli, P.; Paoletti, B.; Otranto, D.; Gasser, R.B.
Title Specific identification of Habronema microstoma and Habronema muscae (Spirurida, Habronematidae) by PCR using markers in ribosomal DNA Type Journal Article
Year 2004 Publication Molecular and Cellular Probes Abbreviated Journal Mol Cell Probes
Volume 18 Issue 4 Pages 215-221
Keywords Animals; Base Sequence; DNA, Ribosomal/blood/*genetics; Feces/parasitology; Genetic Markers; Horses/*parasitology; Molecular Sequence Data; Muscidae/*genetics; Polymerase Chain Reaction; Spirurida Infections/genetics; Spiruroidea/*genetics; Stomach/*parasitology
Abstract Gastric or cutaneous habronemosis caused by Habronema microstoma Creplin, 1849 and Habronema muscae Carter, 1865 is a parasitic disease of equids transmitted by muscid flies. There is a paucity of information on the epidemiology of this disease, which is mainly due to limitations with diagnosis in the live animal and with the identification of the parasites in the intermediate hosts. To overcome such limitations, a molecular approach, based on the use of genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal DNA, was established for the two species of Habronema. Characterisation of the ITS-2 revealed sequence lengths and G+C contents of 296 bp and 29.5% for H. microstoma, and of 334 bp and 35.9% for H. muscae, respectively. Exploiting the sequence difference (approximately 40%) between the two species of nematode, primers were designed and tested by the polymerase chain reaction (PCR) for their specificity using a panel of control DNA samples from common equid endoparasites, and from host tissues, faeces or muscid flies. Effective amplification from each of the two species of Habronema was achieved from as little as 10 pg of genomic DNA. Hence, this molecular approach allows the specific identification and differentiation of the DNA from H. microstoma and H. muscae, and could thus provide a molecular tool for the specific detection of Habronema DNA (irrespective of developmental stage) from faeces, skin and muscid fly samples. The establishment of this tool has important implications for the specific diagnosis of clinical cases of gastric and cutaneous habronemosis in equids, and for studying the ecology and epidemiology of the two species of Habronema.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0890-8508 ISBN Medium
Area Expedition Conference
Notes PMID:15271381 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2634
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Author Hutchinson, G.W.; Abba, S.A.; Mfitilodze, M.W.
Title Seasonal translation of equine strongyle infective larvae to herbage in tropical Australia Type Journal Article
Year 1989 Publication Veterinary Parasitology Abbreviated Journal Vet Parasitol
Volume 33 Issue 3-4 Pages 251-263
Keywords Animals; Feces/*parasitology; Horses; Queensland; Seasons; Strongyle Infections, Equine/*parasitology; Strongyloidea/growth & development/*physiology; Strongylus/growth & development/*physiology; Tropical Climate
Abstract Longevity in faeces, migration to and survival on herbage of mixed strongyle infective larvae (approximately 70% cyathostomes: 30% large strongyles) from experimentally deposited horse faeces was studied in the dry tropical region of North Queensland for up to 2 years. Larvae were recovered from faeces deposited during hot dry weather for a maximum of 12 weeks, up to 32 weeks in cool conditions, but less than 8 weeks in hot wet summer. Translation to herbage was mainly limited to the hot wet season (December-March), except when unseasonal winter rainfall of 40-50 mm per month in July and August allowed some additional migration. Survival on pasture was estimated at 2-4 weeks in the summer wet season and 8-12 weeks in the autumn-winter dry season (April-August). Hot dry spring weather (pre-wet season) was the most unfavourable for larval development, migration and survival. Peak counts of up to 60,000 larvae kg-1 dry herbage were recorded. The seasonal nature of pasture contamination allowed the development of rational anthelmintic control programs based on larval ecology.
Address Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville, Australia
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0304-4017 ISBN Medium
Area Expedition Conference
Notes PMID:2815535 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2672
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Author Holzapfel, W.H.; Botha, S.J.
Title Physiology of Sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against Bacillus species Type Journal Article
Year 1988 Publication International Journal of Food Microbiology Abbreviated Journal Int J Food Microbiol
Volume 7 Issue 2 Pages 161-168
Keywords Animals; Bacillaceae/isolation & purification/*physiology; Bacillus/*physiology; Cattle; *Ecology; Feces/*microbiology; Food Microbiology; Horses; Sewage; Sheep; Water Microbiology
Abstract In an ecological study only low numbers of Sporolactobacillus were found in habitats such as the faeces of herbivores, the rumen of cattle and the final waste water of an abattoir. Their presence in the final waste water of an abattoir indicates their possible association with food, and, more specifically, with meat. Differences were found in some physiological characteristics. One isolate (L2404) differed from the authentic Sporolactobacillus ATCC 15538 by its inability to ferment inulin, its growth in presence of 6.5% NaCl and in 0.2% tellurite, by the isomer(s) of lactic acid produced and the mol% G + G in the DNA. One Sporolactobacillus isolate (L2407) showed antagonism against Bacillus cereus, Bacillus cereus var, mycoides, Bacillus megaterium and Bacillus subtilis.
Address Department of Microbiology and Plant Pathology, University of Pretoria, Republic of South Africa
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0168-1605 ISBN Medium
Area Expedition Conference
Notes PMID:3275317 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2675
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Author Hughes, K.L.; Sulaiman, I.
Title The ecology of Rhodococcus equi and physicochemical influences on growth Type Journal Article
Year 1987 Publication Veterinary Microbiology Abbreviated Journal Vet Microbiol
Volume 14 Issue 3 Pages 241-250
Keywords Animals; Feces/microbiology; Horses; Hydrogen-Ion Concentration; Rhodococcus/*growth & development; *Soil Microbiology; Temperature
Abstract Growth of Rhodococcus equi was studied in vitro. Optimal growth occurred under aerobic conditions between pH 7.0 and 8.5, at 30 degrees C. R. equi survived better in a neutral soil (pH 7.3) than it did in two acid soils (pH less than 5.5). It grew substantially better in soils enriched with faeces than in soils alone. Simple organic acids in horse dung, especially acetate and propionate, appear to be important in supporting growth of R. equi in the environment. The ecology of R. equi can be best explained by an environmental cycle allowing its proliferation in dung, influenced by management, grazing behaviour and prevailing climatic conditions. Preventive measures should be aimed at reducing or avoiding focal areas of faecal contamination in the environment.
Address School of Veterinary Science, University of Melbourne, Parkville, Vic., Australia
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0378-1135 ISBN Medium
Area Expedition Conference
Notes PMID:3672866 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2678
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Author Takai, S.; Fujimori, T.; Katsuzaki, K.; Tsubaki, S.
Title Ecology of Rhodococcus equi in horses and their environment on horse-breeding farms Type Journal Article
Year 1987 Publication Veterinary Microbiology Abbreviated Journal Vet Microbiol
Volume 14 Issue 3 Pages 233-239
Keywords Actinomycetales Infections/*veterinary; Animals; Animals, Newborn/*microbiology; *Environmental Microbiology; Feces/microbiology; Female; Horse Diseases/*microbiology; Horses/*microbiology; Rhodococcus/*isolation & purification
Abstract Quantitative culture of R. equi in the feces of dams and foals, in the air of the stalls and in the soil of the paddocks was carried out on three horse-breeding farms during the foaling season. The isolation rates of R. equi from the feces of dams from the 3 farms suddenly increased to approximately 80% at the end of March, when the snow in the paddocks finished melting, and remained at that level during April and May. The mean number of R. equi and the isolation rate of R. equi from the feces of dams on the farms were investigated for 5 weeks before and 5 weeks after delivery. During the 10 weeks, there were no differences in the isolation rate or in the mean number of R. equi from the feces of dams. R. equi was first isolated from the feces of the foals born in February and the middle of March at 3-4 weeks of age, on the other hand, it was first isolated from the feces of foals born in the end of March and April at 1-2 weeks of age. The number of R. equi in the soil collected from the paddocks used by dams during the winter was approximately 10(2)-10(4) g-1 of soil during the experiment. R. equi was isolated from the air in the stalls at the end of March and the number of R. equi in the air increased particularly on dry and windy days.(ABSTRACT TRUNCATED AT 250 WORDS)
Address Department of Animal Hygiene, School of Veterinary Medicine and Animal Science, Kitasato University, Aomori, Japan
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0378-1135 ISBN Medium
Area Expedition Conference
Notes PMID:3672865 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2679
Permanent link to this record
 

 
Author Polley, L.
Title Strongylid parasites of horses: experimental ecology of the free-living stages on the Canadian prairie Type Journal Article
Year 1986 Publication American Journal of Veterinary Research Abbreviated Journal Am J Vet Res
Volume 47 Issue 8 Pages 1686-1693
Keywords Animals; Canada; Ecology; Feces; Female; Horse Diseases/*epidemiology/parasitology; Horses; Larva; Ovum/cytology; Seasons; Strongyloides/isolation & purification; Strongyloidiasis/epidemiology/*veterinary
Abstract Each month for a 1-year period (October through September), equine fecal masses containing eggs of strongylid nematodes were placed outdoors on small grass plots in Saskatchewan, Canada. Thereafter, feces and grass from the plots were sampled after intervals of 1 week or longer, and the strongylid eggs and larvae recovered were counted. These observations were made over a 2-year period. Development of eggs to infective larvae occurred in all experiments, except those established in October, December, and January. Infective larvae from experiments set up in April through September survived that winter. During the summer, there was a gradual build up of infective larvae in the fecal masses, which reached a peak in August and September and then decreased into the winter. These results are discussed in the context of the control of strongylid parasites of horses on the Canadian prairie and in other areas of the world with a similar climate and similar horse management practices.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0002-9645 ISBN Medium
Area Expedition Conference
Notes PMID:3752676 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2682
Permanent link to this record