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Author Passler, S.; Pfeffer, M. openurl 
  Title Detection of antibodies to alphaviruses and discrimination between antibodies to eastern and western equine encephalitis viruses in rabbit sera using a recombinant antigen and virus-specific monoclonal antibodies Type Journal Article
  Year 2003 Publication Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health Abbreviated Journal J Vet Med B Infect Dis Vet Public Health  
  Volume 50 Issue 6 Pages 265-269  
  Keywords Animals; Antibodies, Monoclonal/*immunology; Antibodies, Viral/*analysis/blood; DNA Primers; Encephalitis Virus, Eastern Equine/genetics/*immunology; Encephalitis Virus, Western Equine/genetics/*immunology; Encephalomyelitis, Equine/*diagnosis/*virology; Epitopes; Fluorescent Antibody Technique/*veterinary; Horses; Rabbits; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction/veterinary  
  Abstract Three arthropod-borne alphaviruses, western equine encephalitis viruses (WEEV), eastern equine encephalitis viruses (EEEV) and Venezuelan equine encephalitis viruses are the aetiological agents of a sometimes severe encephalomyelitis in equines and humans in the New World. With regard to the different ecology and epidemiology of these viruses, a method applied in serological screening should be able to distinguish between them as well as other related members of the genus Alphavirus in the American continent. However, this has been hampered in the past by (a) the close antigenic relationship between alphaviruses in traditional serological assays, especially in the routinely used haemagglutination-inhibition, and (b) the need of biosafety level 3 facilities to grow the viral antigens. An epitope blocking assay using an EEEV glycoprotein E1-expressing recombinant Sindbis virus and virus-specific monoclonal antibodies (mAbs) binding to the E1 of EEEV (strain NJ/60) and the E1 of Sindbis virus was established using automated flow cytometry. The test was evaluated using sera of infected and vaccinated rabbits. A cut-off value of 30% inhibition for antigenic complex-specific seroconversion was found to be sufficient for the detection of the respective infection. By using three different mAbs in parallel, we were able to detect alphavirus genus-, EEEV- and WEEV-complex-specific serum antibodies. As this test is based on the inhibition of binding of virus-specific mAbs, sera of every origin other than mouse can be tested. Thus, this assay may prove useful in the serological screening of a variety of animal species during an outbreak investigation.  
  Address Institute for Medical Microbiology, Infectious and Epidemic Diseases, Veterinary Faculty, Ludwig-Maximilians-University, Munich, Germany  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0931-1793 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:14628996 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2639  
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Author Hutchinson, G.W.; Abba, S.A.; Mfitilodze, M.W. openurl 
  Title Seasonal translation of equine strongyle infective larvae to herbage in tropical Australia Type Journal Article
  Year 1989 Publication Veterinary Parasitology Abbreviated Journal Vet Parasitol  
  Volume 33 Issue 3-4 Pages 251-263  
  Keywords Animals; Feces/*parasitology; Horses; Queensland; Seasons; Strongyle Infections, Equine/*parasitology; Strongyloidea/growth & development/*physiology; Strongylus/growth & development/*physiology; Tropical Climate  
  Abstract Longevity in faeces, migration to and survival on herbage of mixed strongyle infective larvae (approximately 70% cyathostomes: 30% large strongyles) from experimentally deposited horse faeces was studied in the dry tropical region of North Queensland for up to 2 years. Larvae were recovered from faeces deposited during hot dry weather for a maximum of 12 weeks, up to 32 weeks in cool conditions, but less than 8 weeks in hot wet summer. Translation to herbage was mainly limited to the hot wet season (December-March), except when unseasonal winter rainfall of 40-50 mm per month in July and August allowed some additional migration. Survival on pasture was estimated at 2-4 weeks in the summer wet season and 8-12 weeks in the autumn-winter dry season (April-August). Hot dry spring weather (pre-wet season) was the most unfavourable for larval development, migration and survival. Peak counts of up to 60,000 larvae kg-1 dry herbage were recorded. The seasonal nature of pasture contamination allowed the development of rational anthelmintic control programs based on larval ecology.  
  Address Graduate School of Tropical Veterinary Science, James Cook University of North Queensland, Townsville, Australia  
  Corporate Author Thesis  
  Publisher Place of Publication (up) Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0304-4017 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:2815535 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2672  
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Author Hardy, J.L. openurl 
  Title The ecology of western equine encephalomyelitis virus in the Central Valley of California, 1945-1985 Type Journal Article
  Year 1987 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg  
  Volume 37 Issue 3 Suppl Pages 18s-32s  
  Keywords Aedes/microbiology; Animals; Birds; California; Culex/microbiology; Encephalitis Virus, Western Equine/*physiology; Encephalomyelitis, Equine/*history/microbiology/transmission/veterinary; History, 20th Century; Horse Diseases/history/transmission; Horses; Humans; Insect Vectors/microbiology; Mammals  
  Abstract Reeves' concept of the summer transmission cycle of western equine encephalomyelitis virus in 1945 was that the virus was amplified in a silent transmission cycle involving mosquitoes, domestic chickens, and possibly wild birds, from which it could be transmitted tangentially to and cause disease in human and equine populations. Extensive field and laboratory studies done since 1945 in the Central Valley of California have more clearly defined the specific invertebrate and vertebrate hosts involved in the basic virus transmission cycle, but the overall concept remains unchanged. The basic transmission cycle involves Culex tarsalis as the primary vector mosquito species and house finches and house sparrows as the primary amplifying hosts. Secondary amplifying hosts, upon which Cx. tarsalis frequently feeds, include other passerine species, chickens, and possibly pheasants in areas where they are abundant. Another transmission cycle that most likely is initiated from the Cx. tarsalis-wild bird cycle involves Aedes melanimon and the blacktail jackrabbit. Like humans and horses, California ground squirrels, western tree squirrels, and a few other wild mammal species become infected tangentially with the virus but do not contribute significantly to virus amplification.  
  Address Department of Biomedical and Environmental Health Sciences, School of Public Health, University of California, Berkeley 94720  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9637 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:3318522 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2677  
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Author Crans, W.J.; McNelly, J.; Schulze, T.L.; Main, A. openurl 
  Title Isolation of eastern equine encephalitis virus from Aedes sollicitans during an epizootic in southern New Jersey Type Journal Article
  Year 1986 Publication Journal of the American Mosquito Control Association Abbreviated Journal J Am Mosq Control Assoc  
  Volume 2 Issue 1 Pages 68-72  
  Keywords Aedes/*microbiology; Alphavirus/*isolation & purification; Animals; Disease Outbreaks/veterinary; Ecology; Encephalitis Virus, Eastern Equine/*isolation & purification; Encephalomyelitis, Equine/epidemiology/transmission/veterinary; Horse Diseases/epidemiology/transmission; Horses; Insect Vectors/microbiology; New Jersey  
  Abstract Eastern equine encephalitis virus (EEE) was isolated from the salt marsh mosquito, Aedes sollicitans, collected from coastal areas of New Jersey on 3 occasions during the late summer and fall of 1982. The isolations were made at a time when local Culiseta melanura were either undergoing a population increase or exhibiting high levels of EEE virus. Although no human cases were reported during the epizootic period, the data lend support to the hypothesis that Ae. sollicitans is capable of functioning as an epidemic vector in the coastal areas of New Jersey where human cases of EEE have been most common.  
  Address Mosquito Research and Control, Cook College, Rutgers University, New Brunswick, NJ 08903  
  Corporate Author Thesis  
  Publisher Place of Publication (up) Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 8756-971X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:2853203 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2684  
Permanent link to this record
 

 
Author Sabattini, M.S.; Monath, T.P.; Mitchell, C.J.; Daffner, J.F.; Bowen, G.S.; Pauli, R.; Contigiani, M.S. openurl 
  Title Arbovirus investigations in Argentina, 1977-1980. I. Historical aspects and description of study sites Type Journal Article
  Year 1985 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg  
  Volume 34 Issue 5 Pages 937-944  
  Keywords Animals; Arbovirus Infections/*epidemiology/microbiology; Arboviruses; Argentina; Birds; Cattle; Child; Climate; Ecology; Encephalitis Virus, St. Louis; Encephalitis Virus, Venezuelan Equine; Encephalitis Virus, Western Equine; Encephalitis, St. Louis/epidemiology/microbiology; Encephalomyelitis, Equine/epidemiology/microbiology/veterinary; Encephalomyelitis, Venezuelan Equine/epidemiology/microbiology/veterinary; Geography; Horse Diseases/epidemiology/microbiology; Horses/microbiology; Humans  
  Abstract This is the introductory paper to a series on the ecology of arboviruses in Argentina. Epizootics of equine encephalitis have occurred since at least 1908, principally in the Pampa and Espinal biogeographic zones, with significant economic losses; human cases of encephalitis have been rare or absent. Both western equine and eastern equine encephalitis viruses have been isolated from horses during these epizootics, but the mosquitoes responsible for transmission have not been identified. A number of isolations of Venezuelan equine encephalitis (VEE) virus were reported between 1936 and 1958 in Argentina, but the validity of these findings has been seriously questioned. Nevertheless, serological evidence exists for human infections with a member of the VEE virus complex. Serological surveys conducted in the 1960s indicate a high prevalence of infection of humans and domestic animals with St. Louis encephalitis (SLE), and 2 SLE virus strains have been isolated from rodents. Human disease, however, has rarely been associated with SLE infection. Only 7 isolations of other arboviruses have been described (3 of Maguari, 1 of Aura, 2 of Una, and 1 of an untyped Bunyamwera group virus). In 1977, we began longitudinal field studies in Santa Fe Province, the epicenter of previous equine epizootics, and in 1980 we extended these studies to Chaco and Corrientes provinces. The study sites are described in this paper.  
  Address  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9637 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:4037184 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2685  
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Author Mitchell, C.J.; Darsie, R.F.J.; Monath, T.P.; Sabattini, M.S.; Daffner, J. openurl 
  Title The use of an animal-baited net trap for collecting mosquitoes during western equine encephalitis investigations in Argentina Type Journal Article
  Year 1985 Publication Journal of the American Mosquito Control Association Abbreviated Journal J Am Mosq Control Assoc  
  Volume 1 Issue 1 Pages 43-47  
  Keywords Animals; Argentina; *Culicidae/classification; Disease Outbreaks/veterinary; Encephalitis Virus, Western Equine; Encephalomyelitis, Equine/transmission/*veterinary; Entomology/*instrumentation; Equipment Design; Horse Diseases/*transmission; Horses; *Insect Vectors; Population Surveillance  
  Abstract A large net trap was used to sample mosquito populations attracted to horses at three sites each in Santa Fe and Rio Negro Provinces, Argentina, during the austral summer of 1984. These provinces, as well as others in Argentina, were affected by a severe epizootic of western equine encephalitis (WEE) during 1982-83. Totals of 2,752 and 6,929 mosquitoes were collected in Santa Fe and Rio Negro Provinces during five and three trap nights, respectively. Culex mosquitoes of the subgenus Culex were predominant (45.8% of total) in the Santa Fe collections, although Aedes albifasciatus also was prevalent (21.7%). The latter species was predominant (95.7% of total) in the Rio Negro collections. The mosquito fauna was less complex (minimum of 6 species) in Rio Negro Province as compared to Santa Fe Province (minimum of 18 species). The advantages of the net trap indicate that this trap can become a useful tool in arbovirus ecology studies in other areas.  
  Address Division of Vector-Borne Viral Diseases, Centers for Disease Control, Fort Collins, CO 80522-2087  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 8756-971X ISBN Medium  
  Area Expedition Conference  
  Notes PMID:2906656 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2686  
Permanent link to this record
 

 
Author Scherer, W.F.; Madalengoitia, J.; Flores, W.; Acosta, M. openurl 
  Title Ecologic studies of Venezuelan encephalitis virus in Peru during 1970-1971 Type Journal Article
  Year 1975 Publication American Journal of Epidemiology Abbreviated Journal Am J Epidemiol  
  Volume 101 Issue 4 Pages 347-355  
  Keywords Animals; Antibodies, Viral; Cricetinae/immunology; Culicidae/microbiology; *Disease Vectors; Ecology; *Encephalitis Virus, Venezuelan Equine/immunology/isolation & purification; Encephalomyelitis, Equine/immunology/microbiology/transmission; Female; Hemagglutination Inhibition Tests; Horses/immunology; Humans; Neutralization Tests; Peru  
  Abstract Venezuelan encephalitis (VE) virus has intermittently produced epidemics and equine epizootics on the dry Pacific coastal plain of Peru since at least the 1930's. However, evidence that the virus exists in the Amazon region of Peru to the east of the Andes mountains was not obtained until antibodies were found in human sera collected in 1965, and 10 strains of the virus were isolated in a forest near the city of Iquitos, Peru during February and March 1971. Eight strains came from mosquitoes and two from dead sentinel hamsters. Three hamsters exposed in forests near Iquitos developed VE virus antibodies suggesting that hamster-benign strains also exist there. Antibody tests of equine sera revealed no evidence that VE virus was actively cycling during the late 1950's or 1960's in southern coastal Peru, where equine epizootics had occurred in the 1930's and 1940's. In northern coastal Peru bordering Ecuador, antibodies were present in equine sera, presumably residual from the 1969 outbreak caused by subtype I virus, since neutralizing antibody titers were higher to subtype I virus than to subtypes III or IV. No VE virus was detected in this northern region during the dry season of 1970 by use of sentinel hamsters. The possibility is considered that VE epidemics and equine epizootics on the Pacific coast of Peru are caused by movements of virus in infected vertebrates traversing Andean passes or in infected vertebrates or mosquitoes carried in airplanes from the Amazon region.  
  Address  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9262 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:235838 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2705  
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Author Sudia, W.D.; Fernandez, L.; Newhouse, V.F.; Sanz, R.; Calisher, C.H. openurl 
  Title Arbovirus vector ecology studies in Mexico during the 1972 Venezuelan equine encephalitis outbreak Type Journal Article
  Year 1975 Publication American Journal of Epidemiology Abbreviated Journal Am J Epidemiol  
  Volume 101 Issue 1 Pages 51-58  
  Keywords Animals; Arboviruses/isolation & purification; Culicidae/microbiology; Disease Vectors/*microbiology; Ecology; Encephalitis Virus, St. Louis/isolation & purification; Encephalitis Virus, Venezuelan Equine/*isolation & purification; Encephalitis Virus, Western Equine/isolation & purification; Encephalomyelitis, Equine/epidemiology/*transmission/veterinary; Horse Diseases/epidemiology/*transmission; Horses; Insect Vectors/microbiology; Mexico  
  Abstract Virus vector studies were conducted in the States of Durango, Chihuahua, and Tamaulipas, Mexico, in June and July 1972. Apparently only a low level of Venzuelan equine encephalitis (VEE) virus transmission to equines occured at the time of the study, and the infection was restricted to areas which had not experienced overt activity during the preceding year. The low level of infection was associated with a scarcity of mosquitoes. The IB (epidemic) strain of VEE virus was isolated from two pools of Anopheles pseudopunctipennis (Theo.) and the blood of one symptomatic equine. The low mosquito population, the relatively few equine cases observed, and the absence of reports of VEE human disease from the outbreak area suggested VEE virus persistence through a low-level mosquito-equine transmission cycle. Other studies have already indicated that wild vertebrates play no more than a minor role in outbreaks of epidemic VEE. Mosquito collections made in areas of the states of Durango, Chihuahua, and Tamaulipas, where considerable epidemic activity of VEE had occurred in 1971, failed to reveal evidence of VEE virus persistence. Twenty-nine ioslations of other arboviruses were also made in these studies: including 22 of St. Louis encephalitis virus (SLE), 2 of Flanders virus, 1 of Turlock virus, 1 of Trivittatus virus of the California Group, 1 of western equine encephalitis virus (VEE), and 2 (from Santa Rose) which possibly represent a hitherto unknown virus in the Bunyamwera Group. These are the first reports of SLE virus isolations from mosquitoes in Mexico, and the first demonstration of Trivittatus, VEE Turlock and Flanders viruses in Mexico from any source.  
  Address  
  Corporate Author Thesis  
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  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9262 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:235213 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2706  
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Author Bast, T.F.; Whitney, E.; Benach, J.L. openurl 
  Title Considerations on the ecology of several arboviruses in eastern Long Island Type Journal Article
  Year 1973 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg  
  Volume 22 Issue 1 Pages 109-115  
  Keywords Animals; Antibodies, Viral/analysis; Arboviruses/*isolation & purification; Birds; Brain/microbiology; Ecology; Encephalitis Virus, St. Louis/immunology/isolation & purification; Encephalitis Virus, Western Equine/immunology/isolation & purification; Encephalitis Viruses/immunology/isolation & purification; Encephalitis Viruses, Tick-Borne/immunology/isolation & purification; Encephalomyelitis, Equine/epidemiology/veterinary; Hemagglutination Inhibition Tests; Horse Diseases/epidemiology; Horses; Humans; Insects; Liver/microbiology; Mites; Neutralization Tests; New York; Snakes; Ticks  
  Abstract  
  Address  
  Corporate Author Thesis  
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  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9637 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:4684881 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2715  
Permanent link to this record
 

 
Author Scherer, W.F.; Dickerman, R.W. openurl 
  Title Ecologic studies of Venezuelan encephalitis virus in southeastern Mexico. 8. Correlations and conclusions Type Journal Article
  Year 1972 Publication The American Journal of Tropical Medicine and Hygiene Abbreviated Journal Am J Trop Med Hyg  
  Volume 21 Issue 2 Pages 86-89  
  Keywords Animals; Birds; Cattle; Chiroptera; Cricetinae; Culex; Culicidae; *Disease Reservoirs; Ecology; Encephalitis Virus, Venezuelan Equine/immunology; Encephalitis Viruses/*isolation & purification; Encephalomyelitis, Equine/epidemiology/*etiology; Horses; Humans; *Insect Vectors; Mammals; Mexico; Mice; Opossums; Rats; Swine  
  Abstract  
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication (up) Editor  
  Language English Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN 0002-9637 ISBN Medium  
  Area Expedition Conference  
  Notes PMID:4399844 Approved no  
  Call Number Equine Behaviour @ team @ Serial 2721  
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