| Home | << 1 2 3 4 5 6 7 8 9 10 >> [11–20] |
|
| Records | |||||
|---|---|---|---|---|---|
| Author | Rehren, K. D. | ||||
| Title | Untersuchung der „Schiefe“ des Pferdes:Symmetrie von Bewegungsablauf und Hufbelastung | Type | Book Whole | ||
| Year | 2018 | Publication | Wissenschaftliche Reihe der Klinik für Pferde | Abbreviated Journal | |
| Volume | Issue | Pages | |||
| Keywords | |||||
| Abstract | Ziel der Studie war die Beschreibung der motorischen Lateralität („Schiefe“) gesunder adulter Pferde. Hierzu wurden 14 lahmfreie Pferde (10 Warmblüter, 4 Quarter Horses) auf dem Laufband in Schritt und Trab auf individuelle Abweichungen von der symmetri-schen Bewegung hinsichtlich Abstellung der Hinterhand, Wirbelsäulenform (Biegung), Vor- und Rückführung sowie Belastung von Vorder- und Hintergliedmaßen jeweils simul-tan hochfrequenzkinematographisch und kinetisch mittels resistiver Hufdrucksensoren untersucht. Zusätzlich wurden bevorzugte Abstellung und Biegung sowie Galopppräfe-renz klinisch bzw. reiterlich beurteilt, das Vorliegen einer Weideschrittpräferenz unter-sucht und Freilaufversuche (bevorzugte Abbiegerichtung, Ausweichrichtung um ein Hin-dernis, Galoppräferenz) durchgeführt. Keines der Pferde zeigte Symmetrie über alle Merkmale; zwar wurde bei jedem Merkmal für einzelne Probanden Symmetrie festgestellt, mit Ausnahme des Galopp-Freilaufver-suchs trat Asymmetrie jedoch häufiger auf. 12 Probanden zeigten in der Bewegungsana-lyse eine signifikante seitliche Abstellung (8x rechts (R), 4x links (L), mittlere seitliche Abweichung im Schritt 1,22°±1,09°, im Trab 1,05°±1,03°), 10 eine bevorzugte Biegung (9x L, 1x R), 13 signifikante Asymmetrien der Gliedmaßenvor- bzw. -rückführung und 12 eine signifikante asymmetrische Gliedmaßenbelastung für die normalisierte Spitzenkraft (PFN, peak force) bzw. den Impuls der Belastung (IN) an mindestens einem Beinpaar (alle Merkmale individuell wiederholbar). Linksabgestellte Pferde führten in beiden Gang-arten das rechte Hinterbein signifikant weiter zurück als nach rechts abgestellte Pferde, die tendenziell eher das linke Hinterbein weiter zurückführten. – Die auf dem Laufband festgestellten Asymmetrien traten in beiden Gangarten auf, aber nicht immer überein-stimmend, wobei die Richtung der bevorzugten Abstellung und ebenso der bevorzugten Biegung beim einzelnen Pferd in Schritt und Trab nie gegensätzlich waren. Jeder dieser beiden Parameter wurde daher für das einzelne Pferd über beide Gangarten zusammen-gefasst. Die Richtung von Abstellung und Biegung waren voneinander unabhängig, so-dass die Bewegungsmuster Abstellung ohne Biegung, Biegung ohne Abstellung, Abstel-lung mit Biegung in Laufrichtung (Travers) sowie Abstellung mit Biegung entgegen der Laufrichtung (Schulterherein) vorkamen. Kein Pferd war in beiden Merkmalen symmet-risch; für manche Pferde war neben der einfachen Biegung auch eine S-Form der Wir-belsäule signifikant. Die Belastungssymmetrie der Gliedmaßen war in beiden Gangarten von der Abstellung und der Biegung unabhängig, es gab aber Hinweise auf Zusammen-hänge zwischen bestimmten Kombinationen von Abstellung und Biegung einerseits und der Existenz einer Belastungsasymmetrie der Vorderbeine im Trab andererseits (unge-bogen mit Abstellung laufende und im Schulterherein laufende Pferde belasteten i.d.R. asymmetrisch). 8 Pferde wiesen eine signifikante Weideschrittpräferenz auf (5x L, 3x R), die bei ebenso vorhandener seitlicher Abstellung signifkant häufiger auf der der Abstellung abgewandten Seite auftrat. Sie wies keinen Zusammenhang mit der Rückführung der Hinterbeine im Schritt oder im Trab auf. Das Auftreten einer Weideschrittpräferenz war signifikant vom durch Abstellung und Biegung bestimmten Bewegungsmuster abhängig (Auftreten bei Abstellung ohne Biegung und bei Schulterherein, nicht bei Travers oder Biegung ohne Abstellung). Zudem zeigt die Präferenz der Pferde im Weideschritt eine starke Überein-stimmung mit der Belastungssymmetrie der Vorderbeine im Trab (bevorzugt vorgestelltes Bein wird mehr belastet (IN); symmetrische Belastung bei fehlender Präferenz). Im Hindernisversuch hatten 12 von 13 getesteten Probanden eine signifikant bevorzugte Ausweichrichtung (5x L, 7x R); diese war unabhängig von der gemessenen Wirbelsäulenform, lag aber bei Pferden mit seitlicher Abstellung überwiegend auf der Seite der gemessenen Abstellung. Im Abbiegeversuch zeigten 6 von 12 getesteten Probanden eine signifikant bevorzugte Abbiegerichtung (3x L, 3x R), diese war weder von der Abstellungsrichtung noch von der Wirbelsäulenform, der Weideschrittpräferenz oder der Belastungssymmetrie der Vorderbeine im Trab abhängig. Die meisten Pferde zeigten im Freilauf keine signifikante Galopppräferenz (2x L, 11x gerade (N), 1x R), wogegen reiterlich bei den meisten Pferden eine Präferenz festgestellt wurde; beide Beurteilungen stimmten ebenso wie die Beurteilungen verschiedener Reiter nicht miteinander überein. Die gemessene Abstellungsrichtung ließ sich durch visuelle Beobachtung mit hoher Si-cherheit vorhersagen. Die bevorzugte Wirbelsäulenform stimmte nicht mit der reiterlich oder an der Longe festgestellten Biegung überein, wohl aber mit der Seite, auf die die Mähne fällt. Die vorliegende Studie weist nach, dass individuell stabile motorische Lateralität, weitge-hend bestehend aus den den Merkmalen der reiterlich postulierten „Schiefe“, bei gesun-den Pferden in symmetrischen Gangarten besteht und dass zwischen bestimmten Merkmalen vorhersagbare Zusammenhänge bestehen. Daraus ergeben sich wertvolle Schlussfolgerungen für das reiterliche Geraderichten des Pferdes, für das in Übereinstimmung mit der Reitliteratur insbesondere die Lektion Schulterherein geeignet erscheint. Es ist gelungen, für die wesentlichen Merkmale Abstellung, Biegung und Belastungssymmetrie der Vordergliedmaßen im Trab klinische Tests aufzuzeigen, die mit hoher Sicher-heit die Ergebnisse der Bewegungsanalyse vorhersagen können, wobei Rückschlüsse auf weitere asymmetrische Merkmale möglich sind. Die reiterliche Beurteilung von bevor-zugter Biegung und Galopppräferenz scheint jedoch von weiteren Faktoren außer dem Pferd selbst abhängig zu sein und stimmt häufig nicht mit den messbaren Parametern überein. – In dieser Studie wird erstmals eine wissenschaftlich überprüfte und klinisch anwendbare Methode zur umfassenden Analyse der motorischen Lateralität im Sinne der „Schiefe“ eines Pferdes beschrieben. Summary -“Crookedness“ in the Horse: Symmetry of Motion and Hoof Loading The purpose of this study was description of motor laterality (in terms of “crookedness“) of sound adult horses. 14 non-lame horses (10 Warmblood horses, 4 Quarter Horses) were examined walking and trotting on a treadmill for individual motion asymmetry concerning keeping the hindlimbs at an angle (hindlimbs not tracking the frontlimbs, “hindquarter angle”), lateroflexion, pro- and retraction of the limbs as well as loading of front- and hindlimbs. High-speed-kinematography and resistive pressure sensors were used simultanously. The horses were also ridden to evaluate preferred lateroflexion and preferred lead in canter. Moving at an angle towards a preferred side was assessed visually. Laterality of grazing stance was tested, as well as preferred turning direction, obstacle avoidance direction and preferred lead in canter while moving unrestrained in an arena. None of the horses moved symetrically over all traits; although there were symmetrical traits with individual horses, asymmetry was more commen (except for the unrestrained preferred lead test). On the treadmill, 12 horses moved with their hindquarters significantly at a angle (8x right (R), 4x left (L), mean lateral angle 1,22°±1,09° at a walk and 1,05°±1,03° at a trot), 10 showed laterality for lateroflexion (9x L, 1x R), 13 had significant asymmetries for protraction and/ or retraction and 12 for loading of contralateral limbs concerning normalized peak force (PFN) and impulse (IN), respectively, of at least one pair of limbs (all with good repeatibility). Horses keeping their hindquarters to the left showed significantly larger retraction of the right hindlimb compared to horses keeping their hindquarters to the right, who tended to further retract the left hindlimb. – Asymmetries found on the treadmill occurred in both gaits but were not always the same in both gaits, though the direction of the hindquarter angle as well as that of the preferred lateroflexion never contradicted each other within the same individual. Therefore, a specific laterality direction of hindquarter angle and lateroflexion could be assigned to each horse. The direction of preferred hindquarter angle and lateroflexion, respectively, were independent of each other so that the movement patterns “hindquarter angle without lateroflexion”, “lateroflexion without hindquarter angle”, “hindquarter angle with lateroflexion to the same side” (hindquarter-in/ travers) and “hindquarter angle with lateroflexion to opposite sides” (shoulder-in) could be found; no horse moved symmetrical for both traits. The lateroflexion of some horses could even be regarded as a double bend. At both gaits, symmetry of limb loading was independent of hindquarter angle as well as lateroflexion, taken seperately; still, there seems to be a relation between certain combinations of hindquarter angle and lateroflexion, on the one side, and the existence or absence of asymmetric loading of the frontlimbs in trot (horses moving with hindquarter angle, but without lateroflexion, and horses moving in shoulder-in mostly had asymmetric frontlimb loading). 238 7. Summary8 horses displayed laterality for a grazing stance (5x L, 3x R); their hindquarter angle, if they showed one, was significantly more often on the opposite side of their advanced frontleg while grazing. The direction of the grazing stance was independent of hindlimb retraction symmetry in either gait. Occurrence of grazing stance laterality significantly depended on the movement pattern consisting of hindquarter angle and lateroflexion (horses moving with a hindquarter angle, but without lateroflexion, and horses moving in shoulder-in mostly showed grazing stance laterality, while horses moving with lateroflexion, but without a hindquarter angle, and horses moving in hindquarter-in did not). Also, grazing stance laterality was highly predictive of the symmetry of frontlimb loading at trot (advanced frontleg has significantly higher impulse (IN) at trot; horses without grazing stance laterality showed symmetrical impulse). 12 of 13 horses displayed laterality for obstacle avoidance direction (5x L, 7x R) that was independent of the individually preferred lateroflexion but if the horses showed a hindquarter angle it was usually to the same side. 6 of 12 horses showed laterality for turning direction (3x L, 3x R) that had no relation to laterality of either hindquarter angle, lateroflexion, grazing stance or frontlimb loading symmetry at trot. Most horses had no significant lead preference in the unrestrained canter test (2x L, 11x no preference (N), 1x R) even though riders assigned a lead preference to most horses; both ratings did not match, as well as the rating between two different riders did not match. The (measured) direction of hindquarter angle was highly predictable by visual asessment. Preferred (measured) lateroflexion did not match the direction of lateroflexion assigned by either rider or on the long line; it was in agreement with the mane side of the horse, though. This study proves that individual and stable motor laterality exists in sound horses in symmetrical gaits, mostly consisting of the traits that are hypothesized as “crookedness” in riding literature. It also shows that predictible relationships exist between certain traits thereof. This knowledge is valuable since it has implications for the training of riding horses for straightness, wherefore especially riding shoulder-in should be helpful. Relatively easy clinical tests could successfully be found that allow well predicting main features of individual horses ́ motor laterality like hindquarter angle, lateroflexion and frontlimb loading symmetry at trot as measured on the treadmill. Some conclusions can also be drawn for other asymmetrical traits. The asessment of preferred lateroflexion and canter lead performed by riders, though, seems to also underlie other influences than the horse itself and failed to be a predictor of the measured traits. – For the first time, a scientifically proven and clinically applicable method of asessing a horses motor laterality in terms of “crookedness” is presented. |
||||
Address  |
http://dnb.d-nb.de | ||||
| Corporate Author | Thesis | Ph.D. thesis | |||
| Publisher | Cuvillier Verlag | Place of Publication | Göttingen | Editor | Karsten Feige, Peter Stadler,Harald Sieme, Bernhard Ohnesorge |
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | ISBN | 978-3-7369-9804-9 | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6654 | ||
| Permanent link to this record | |||||
| Author | Sigurjónsdóttir, H.; Haraldsson, H. | ||||
| Title | Significance of Group Composition for the Welfare of Pastured Horses | Type | Journal Article | ||
| Year | 2019 | Publication | Animals | Abbreviated Journal | Animals |
| Volume | 9 | Issue | 14 | Pages | |
| Keywords | horse welfare; aggression; allogrooming; pastured horses; Icelandic horse | ||||
| Abstract | We explore how herd composition and management factors correlate with frequencies of social interactions in horse groups. Since the welfare of horses correlates with low aggression levels and social contact opportunities, information of this kind is important. The data are a collection of records of social interactions of 426 Icelandic horses in 20 groups of at least eight horses. The complexities and limitations of the data prohibit useful statistical modelling so the results are presented descriptively. Interesting and informative patterns emerge which can be of use both in management and in future studies. Of special interest are the low levels of agonistic behaviours in breeding groups where one stallion was present. The horses were less agonistic when in groups with young foals and where group membership was stable. Unfamiliar yearlings in peer groups were especially aggressive. Allogrooming was most frequent in groups with relatively more young horses and in unstable and small groups. Interestingly, the horses allogroomed more if they had few preferred allogrooming partners. The findings show that composition (age/sex) and stability of groups are of great importance with respect to aggression levels and opportunities for establishing bonds. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | Animals | ||
| Series Volume | 9 | Series Issue | 1 | Edition | |
| ISSN | 2076-2615 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6510 | ||
| Permanent link to this record | |||||
| Author | Stecken, Paul | ||||
| Title | Bemerkungen und Zusammenhänge | Type | Journal Article | ||
| Year | 2015 | Publication | Abbreviated Journal | ||
| Volume | Issue | Pages | |||
| Keywords | |||||
| Abstract | |||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | FNverlag der Deutschen Reiterlichen Vereinigung GmbH | Place of Publication | wARENDORF | Editor | |
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | ISBN | 978-3-88542-889-3 | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6511 | ||
| Permanent link to this record | |||||
| Author | Murphy, J.; Arkins, S. | ||||
| Title | Laterality and visuo-spatial ability in the equine: Functional measures of sport horse selection? | Type | Journal Article | ||
| Year | 2006 | Publication | BSAP Occasional Publication | Abbreviated Journal | BSAP Occasional Publication |
| Volume | 35 | Issue | Pages | 159-170 | |
| Keywords | |||||
| Abstract | Laterality in any organism or species can be manifest as morphological, sensory and functional degrees of asymmetry such as hemispheric dominance, handedness or sidedness and other motor functional behaviours and as such is equally important in equitation. The influence of the horses' sex on both the direction and the degree of the laterality was explored within and between 4 experimental procedures in the 1st study. The findings showed that the direction, but not the degree of idiosyncratic motor preference in the horses was strongly sex-related. Male horses exhibited significantly more left lateralized responses and female horses exhibited significantly more right lateralized responses. Visuo-spatial ability is also likely to be important in the performance horse. In many species, moderate to large differences in visuo-spatial ability have been reported between the sexes, with superior visuo-spatial ability being reported in males of all species investigated to date. As no known studies had addressed visuo-spatial ability in the equine, the objective of the 2nd study, was to determine if visuo-spatial ability differed between male and female horses. The results produced the first behavioural demonstration of superior visuo-spatial ability in male horses, similar to that reported in other species. There is evidence to suggest that visuospatial ability and motor laterality are associated with cerebral hemispheric asymmetry and may be intrinsically linked. Brain development and laterality have also been associated with hair patterning, and, in a 3rd study we attempted to identify predictors of lateral bias in motor behaviour in horses. We investigated the relationship between the direction of facial hair whorl rotation and the incidence/direction of laterality in the horse. The findings suggest that direction of facial hair whorl rotation may be a useful indicator of lateralised motor behavioural preferences in the horse. We then attempted to establish if laterality was evident at birth in a 4th study, where we explored if neonatal foals exhibited lateralised patterns during and immediately post the birthing process that were correlated with their facial hair whorl patterns. The results showed a significant association between the sex of the foal and the choice of foreleg presented initially during 2nd stage parturition. Significantly more colt foals led with the left foreleg and significantly more filly foals led with the right foreleg than expected purely by random and the behaviour was correlated with facial hair whorl patterns. The findings also suggest that lateralisation in the horse is determined in utero as has also been shown in humans. Comparisons of wholly intact male and female horses are warranted as they might elucidate additional linkages between motor behaviour, visuo-spatial ability and brain organisation and development in the horse. Further research in this area could lead to more appropriate competition conditions (better fence design/construction on cross-country tracks) and so eliminate unnecessary levels of risk associated with many equestrian sports. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | Cambridge University Press | Place of Publication | Editor | ||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | 2018/02/27 | ||
| ISSN | 0263-967x | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6512 | ||
| Permanent link to this record | |||||
| Author | Bonin, S.J.; Clayton, H.M.; Lanovaz, J.L.; Johnston, T. | ||||
| Title | Comparison of mandibular motion in horses chewing hay and pellets | Type | Journal Article | ||
| Year | 2007 | Publication | Equine Veterinary Journal | Abbreviated Journal | Equine Vet. J. |
| Volume | 39 | Issue | 3 | Pages | 258-262 |
| Keywords | horse; temporomandibular joint; mastication; kinematics | ||||
| Abstract | Summary Reasons for performing study: Previous studies have suggested that temporomandibular joint (TMJ) kinematics depend on the type of food being masticated, but accurate measurements of TMJ motion in horses chewing different feeds have not been published. Hypothesis: The temporomandibular joint has a larger range of motion when horses chew hay compared to pellets. Methods: An optical motion capture system was used to track skin markers on the skull and mandible of 7 horses as they chewed hay and pellets. A virtual marker was created on the midline between the mandibles at the level of the 4th premolar teeth to represent the overall motion of the mandible relative to the skull during the chewing cycle. Results: Frequency of the chewing cycles was lower for hay than for pellets. Excursions of the virtual mandibular marker were significantly larger in all 3 directions when chewing hay compared to pellets. The mean velocity of the virtual mandibular marker during the chewing cycle was the same when chewing the 2 feeds. Conclusions: The range of mediolateral displacement of the mandible was sufficient to give full occlusal contact of the upper and lower dental arcades when chewing hay but not when chewing pellets. Potential relevance: These findings support the suggestion that horses receiving a diet high in concentrate feeds may require more frequent dental prophylactic examinations and treatments to avoid the development of dental irregularities associated with smaller mandibular excursions during chewing. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | American Medical Association (AMA) | Place of Publication | Editor | ||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0425-1644 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | doi: 10.2746/042516407X157792 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 6513 | ||
| Permanent link to this record | |||||
| Author | Palm, A.-K.E.; Wattle, O.; Lundström, T.; Wattrang, E. | ||||
| Title | Secretory immunoglobulin A and immunoglobulin G in horse saliva | Type | Journal Article | ||
| Year | 2016 | Publication | Veterinary Immunology and Immunopathology | Abbreviated Journal | Vet. Immunol. Immunolpathol. |
| Volume | 180 | Issue | Pages | 59-65 | |
| Keywords | Equine; Secretory IgA; IgG; Saliva; Mucosal immunity | ||||
| Abstract | This study aimed to increase the knowledge on salivary antibodies in the horse since these constitute an important part of the immune defence of the oral cavity. For that purpose assays to detect horse immunoglobulin A (IgA) including secretory IgA (SIgA) were set up and the molecular weights of different components of the horse IgA system were estimated. Moreover, samples from 51 clinically healthy horses were tested for total SIgA and IgG amounts in saliva and relative IgG3/5 (IgG(T)) and IgG4/7 (IgGb) content were tested in serum and saliva. Results showed a mean concentration of 74μg SIgA/ml horse saliva and that there was a large inter-individual variation in salivary SIgA concentration. For total IgG the mean concentration was approx. 5 times lower than that of SIgA, i.e. 20μg IgG/ml saliva and the inter-individual variation was lower than that observed for SIgA. The saliva-serum ratio for IgG isotypes IgG3/5 and IgG4/7 was also assessed in the sampled horses and this analysis showed that the saliva-serum ratio of IgG4/7 was in general approximately 4 times higher than that of IgG3/5. The large inter-individual variation in salivary SIgA levels observed for the normal healthy horses in the present study emphasises the need for a large number of observations when studying this parameter especially in a clinical setting. Moreover, our results also indicated that some of the salivary IgG does not originate from serum but may be produced locally. Thus, these results provide novel insight, and a base for further research, into salivary antibody responses of horses. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0165-2427 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6514 | ||
| Permanent link to this record | |||||
| Author | Maury, M.; Murphy, K.; Kumar, S.; Mauerer, A.; Lee, G. | ||||
| Title | Spray-drying of proteins: effects of sorbitol and trehalose on aggregation and FT-IR amide I spectrum of an immunoglobulin G | Type | Journal Article | ||
| Year | 2005 | Publication | European Journal of Pharmaceutics and Biopharmaceutics | Abbreviated Journal | Eur. J. Pharm. Biopharm. |
| Volume | 59 | Issue | 2 | Pages | 251-261 |
| Keywords | Immunoglobulin; Spray-drying; Stabilization; Sorbitol; Trehalose; Water replacement | ||||
| Abstract | An immunoglobulin G (IgG) was spray-dried on a Büchi 190 laboratory spray-dryer at inlet and outlet air temperatures of 130 and 190°C, respectively. The IgG solution contains initially 115mg/ml IgG plus 50mg/ml sorbitol. After dialysis, at least 80% of low molecular weight component was removed. After spray-drying the dialyzed IgG and immediate redissolution of the powder, an increase in aggregates from 1 to 17% occurred. A major shift towards increase β-sheet structure was detected in the spray-dried solid, which, however, reverted to native structure on redissolution of the powder. A correlation between aggregation determined by size exclusion chromatography and alterations in secondary structure determined by Fourier transformation infra-red spectroscopy could not therefore be established. On spray-drying a non-dialyzed, sorbitol-containing IgG only some 0.7% aggregates were formed. The sorbitol is therefore evidently able to stabilize partially the IgG during the process of spray-drying. Addition of trehalose to the liquid feed produced quantitatively the same stabilizing action on the IgG during spray-drying as did the sorbitol. This finding again points towards a water replacement stabilization mechanism. The IgG spray-dried powder prepared from the dialyzed liquid feed showed continued substantial aggregation on dry storage at 25°C. This was substantially less in the non-dialyzed, sorbitol-containing spray-dried powder. Addition of trehalose to both dialyzed and non-dialyzed system produced substantial improvement in storage stability and reduction in aggregate formation in storage. The quantitative stabilizing effect of the trehalose was only slightly higher than that of the sorbitol. Taken together, these results indicate that both the sorbitol and trehalose stabilize the IgG primarily by a water replacement mechanism rather than by glassy immobilization. The relevance of this work is its questioning of the importance of the usually considered dominance of glassy stabilization of protein in dried systems of high glass transition temperature, such as trehalose. The low glass transition temperature sorbitol produces almost equal process and storage stability in this case. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0939-6411 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6515 | ||
| Permanent link to this record | |||||
| Author | Pawluski, J.; Jego, P.; Henry, S.; Bruchet, A.; Palme, R.; Coste, C.; Hausberger, M. | ||||
| Title | Low plasma cortisol and fecal cortisol metabolite measures as indicators of compromised welfare in domestic horses (Equus caballus) | Type | Journal Article | ||
| Year | 2017 | Publication | Plos One | Abbreviated Journal | Plos One |
| Volume | 12 | Issue | 9 | Pages | e0182257 |
| Keywords | |||||
| Abstract | The hypothalamic-pituitary-adrenal (HPA) axis response to chronic stress is far from straight forward, particularly with regards to animal welfare. There are reports of no effect as well as both decreases and increases in cortisol after chronic stressors. Therefore, the first aim of the present study was to determine how measures of compromised welfare, such as chronic pain and haematological anomalies, related to cortisol levels in domestic horses (Equus caballus). Domestic horses are an informative model to investigate the impact of chronic stress (due to environment, pain, work, housing conditions...) on the HPA axis. The second aim was to determine whether levels of fecal cortisol metabolites (FCM) may be used as an indicator of welfare measures. The present study used fifty-nine horses (44 geldings and 15 mares), from three riding centres in Brittany, France. The primary findings show that horses whose welfare was clearly compromised (as indicated by an unusual ears backward position, presence of vertebral problems or haematological anomalies, e.g. anaemia) also had lower levels of both FCM and plasma cortisol. This work extends our previous findings showing that withdrawn postures, indicators of depressive-like behavior in horses, are associated with lower plasma cortisol levels. We also found that evening plasma cortisol levels positively correlated with FCM levels in horses. Future research aims to determine the extent to which factors of influence on welfare, such as living conditions (e.g. single stalls versus group housing in pasture or paddocks), early life factors, and human interaction, act as mediators of cortisol levels in horses. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | Public Library of Science | Place of Publication | Editor | ||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | ISBN | Medium | |||
| Area | Expedition | Conference | |||
| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6516 | ||
| Permanent link to this record | |||||
| Author | Palme, R. | ||||
| Title | Non-invasive measurement of glucocorticoids: Advances and problems | Type | Journal Article | ||
| Year | 2019 | Publication | Physiology & Behavior | Abbreviated Journal | Physiol. Behav. |
| Volume | 199 | Issue | Pages | 229-243 | |
| Keywords | Glucocorticoids; Non-invasive; Faecal cortisol/corticosterone metabolites; Immunoassays; Physiological/biological validation | ||||
| Abstract | Glucocorticoids (GCs; i.e. cortisol/corticosterone) are a central component of the stress response and thus their measurement is frequently used to evaluate the impact of stressful situations. Their metabolites from faeces of various animal species are more and more taken as a non-invasive aid to assess GC release and thus adrenocortical activity. The current literature review includes an extensive collection (1327 papers) and evaluation (see also Supplementary Tables) of the literature on faecal cortisol/corticosterone metabolite (FCM) analysis published to date. It aims at giving reference for researchers interested in implementing FCM analysis into their study or seeking to improve such methods by providing background knowledge on GC metabolism and excretion, conveying insights into methodological issues and stating caveats of FCM analysis and by highlighting prerequisites for and some examples of a successful application of such methods. Collecting faecal samples and analysing FCMs may appear simple and straightforward, but researchers have to select and apply methods correctly. They also need to be aware of the many pitfalls and potentially confounding factors and, last but not least, have to carefully interpret results. Applied properly, measurement of FCMs is a powerful non-invasive tool in a variety of research areas, such as (stress) biology, ethology, ecology, animal conservation and welfare, but also biomedicine. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | Place of Publication | Editor | |||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 0031-9384 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Palme2019_attachment.pdf | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 6517 | ||
| Permanent link to this record | |||||
| Author | Hofmeester, T.R.; Cromsigt, J.P.G.M.; Odden, J.; Andrén, H.; Kindberg, J.; Linnell, J.D.C. | ||||
| Title | Framing pictures: A conceptual framework to identify and correct for biases in detection probability of camera traps enabling multi-species comparison | Type | Journal Article | ||
| Year | 2019 | Publication | Ecology and Evolution | Abbreviated Journal | Ecol Evol |
| Volume | Issue | Pages | |||
| Keywords | animal characteristics; detectability; environmental variables; mammal monitoring; reuse of data; trail camera | ||||
| Abstract | Abstract Obtaining reliable species observations is of great importance in animal ecology and wildlife conservation. An increasing number of studies use camera traps (CTs) to study wildlife communities, and an increasing effort is made to make better use and reuse of the large amounts of data that are produced. It is in these circumstances that it becomes paramount to correct for the species- and study-specific variation in imperfect detection within CTs. We reviewed the literature and used our own experience to compile a list of factors that affect CT detection of animals. We did this within a conceptual framework of six distinct scales separating out the influences of (a) animal characteristics, (b) CT specifications, (c) CT set-up protocols, and (d) environmental variables. We identified 40 factors that can potentially influence the detection of animals by CTs at these six scales. Many of these factors were related to only a few overarching parameters. Most of the animal characteristics scale with body mass and diet type, and most environmental characteristics differ with season or latitude such that remote sensing products like NDVI could be used as a proxy index to capture this variation. Factors that influence detection at the microsite and camera scales are probably the most important in determining CT detection of animals. The type of study and specific research question will determine which factors should be corrected. Corrections can be done by directly adjusting the CT metric of interest or by using covariates in a statistical framework. Our conceptual framework can be used to design better CT studies and help when analyzing CT data. Furthermore, it provides an overview of which factors should be reported in CT studies to make them repeatable, comparable, and their data reusable. This should greatly improve the possibilities for global scale analyses of (reused) CT data. | ||||
| Address |
|||||
| Corporate Author | Thesis | ||||
| Publisher | John Wiley & Sons, Ltd | Place of Publication | Editor | ||
| Language | Summary Language | Original Title | |||
| Series Editor | Series Title | Abbreviated Series Title | |||
| Series Volume | Series Issue | Edition | |||
| ISSN | 2045-7758 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | doi: 10.1002/ece3.4878 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 6518 | ||
| Permanent link to this record | |||||