| Records |
| Author |
Pawluski, J.; Jego, P.; Henry, S.; Bruchet, A.; Palme, R.; Coste, C.; Hausberger, M. |
| Title |
Low plasma cortisol and fecal cortisol metabolite measures as indicators of compromised welfare in domestic horses (Equus caballus) |
Type |
Journal Article |
| Year |
2017 |
Publication |
Plos One |
Abbreviated Journal |
Plos One |
| Volume |
12 |
Issue |
9 |
Pages |
e0182257 |
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| Abstract |
The hypothalamic-pituitary-adrenal (HPA) axis response to chronic stress is far from straight forward, particularly with regards to animal welfare. There are reports of no effect as well as both decreases and increases in cortisol after chronic stressors. Therefore, the first aim of the present study was to determine how measures of compromised welfare, such as chronic pain and haematological anomalies, related to cortisol levels in domestic horses (Equus caballus). Domestic horses are an informative model to investigate the impact of chronic stress (due to environment, pain, work, housing conditions...) on the HPA axis. The second aim was to determine whether levels of fecal cortisol metabolites (FCM) may be used as an indicator of welfare measures. The present study used fifty-nine horses (44 geldings and 15 mares), from three riding centres in Brittany, France. The primary findings show that horses whose welfare was clearly compromised (as indicated by an unusual ears backward position, presence of vertebral problems or haematological anomalies, e.g. anaemia) also had lower levels of both FCM and plasma cortisol. This work extends our previous findings showing that withdrawn postures, indicators of depressive-like behavior in horses, are associated with lower plasma cortisol levels. We also found that evening plasma cortisol levels positively correlated with FCM levels in horses. Future research aims to determine the extent to which factors of influence on welfare, such as living conditions (e.g. single stalls versus group housing in pasture or paddocks), early life factors, and human interaction, act as mediators of cortisol levels in horses. |
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Public Library of Science |
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| Call Number |
Equine Behaviour @ team @ |
Serial |
6516 |
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| Author |
Griebenow, K.; Klibanov, A.M. |
| Title |
Lyophilization-induced reversible changes in the secondary structure of proteins |
Type |
Journal Article |
| Year |
1995 |
Publication |
Proc Natl Acad Sci USA |
Abbreviated Journal |
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| Volume |
92 |
Issue |
24 |
Pages |
10969-10976 |
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| Abstract |
Changes in the secondary structure of some dozen different proteins upon lyophilization of their aqueous solutions have been investigated by means of Fourier-transform infrared spectroscopy in the amide III band region. Dehydration markedly (but reversibly) alters the secondary structure of all the proteins studied, as revealed by both the quantitative analysis of the second derivative spectra and the Gaussian curve fitting of the original infrared spectra. Lyophilization substantially increases the beta-sheet content and lowers the alpha-helix content of all proteins. In all but one case, proteins become more ordered upon lyophilization. |
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Equine Behaviour @ team @ |
Serial |
6519 |
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| Author |
Palme, R.; Touma, C.; Arias, N., Dominchin, M.N.; Lepschy, M. |
| Title |
Steroid extraction: Get the best out of faecal samples |
Type |
Journal Article |
| Year |
2013 |
Publication |
Wiener Tierärztliche Wochenschriften |
Abbreviated Journal |
Wien Tierärztl Monat – Vet Med Austria |
| Volume |
100 |
Issue |
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Pages |
238-246. |
| Keywords |
Review, faeces, extraction, non-invasive hormone monitoring, stress, reproduction. |
| Abstract |
Faecal steroid hormone metabolites are becoming increasingly popular as parameters for reproductive functions and stress. The extraction of the steroids from the faecal matrix represents the initial step before quantification can be performed. The steroid metabolites present in the faecal matrix are of varying polarity and composition, so selection of a proper extraction procedure is essential. There have been some studies to address this complex but often neglected point. Radiolabelled steroids (e.g. cortisol or progesterone) have frequently been added to faecal samples to estimate the efficiency of the extraction procedures used. However, native, unmetabolized steroids are normally not present in the faeces and therefore the results are artifi- cial and do not accurately reflect the actual recoveries of the substances of interest. In this respect, recovery experiments based on faecal samples from radiometabolism studies are more informative. In these samples, the metabolite content accurately reflects the mixture of metabolites present in the given species. As a result, it is possible to evaluate different extraction methods for use with faecal samples. We present studies on sheep, horses, pigs, hares and dogs that utilized samples containing naturally metabolized, 14C-labelled steroids. We recommend extracting faecal steroids by simply suspending the faeces in a high percentage of a primary alcohol (for glucocorticoid metabolites 80% aqueous methanol proved best suited for virtually all mammalian species tested so far). Not only does the procedure significantly increase the total amount of recovered radioactivity, it also increases the percentage of unconjugated metabolites, which are more likely to be recognized by the antibodies used in various immunoassays. The advantages of this extraction procedure are clear: it is very easy to use (no evaporation step is needed), it yields high recoveries and variation based on the extraction procedure is reduced to a minimum. |
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Equine Behaviour @ team @ |
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6520 |
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Gholib, G.; Heistermann, M.; Agil, M.; Supriatna, I.; Purwantara, B.; Nugraha, T.P.; Engelhardt, A. |
| Title |
Comparison of fecal preservation and extraction methods for steroid hormone metabolite analysis in wild crested macaques |
Type |
Journal Article |
| Year |
2018 |
Publication |
Primates |
Abbreviated Journal |
Primates |
| Volume |
59 |
Issue |
3 |
Pages |
281-292 |
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| Abstract |
Since the non-invasive field endocrinology techniques were developed, several fecal preservation and extraction methods have been established for a variety of species. However, direct adaptation of methods from previous studies for use in crested macaques should be taken with caution. We conducted an experiment to assess the accuracy and stability of fecal estrogen metabolite (E1C) and glucocorticoid metabolite (GCM) concentrations in response to several preservation parameters: (1) time lag between sample collection and fecal preservation; (2) long-term storage of fecal samples in 80% methanol (MeOH) at ambient temperature; (3) different degrees of feces drying temperature using a conventional oven; and (4) different fecal preservation techniques (i.e., freeze-drying, oven-drying, and field-friendly extraction method) and extraction solvents (methanol, ethanol, and commercial alcohol). The study used fecal samples collected from crested macaques (Macaca nigra) living in the Tangkoko Reserve, North Sulawesi, Indonesia. Samples were assayed using validated E1C and GCM enzyme immunoassays. Concentrations of E1C and GCM in unprocessed feces stored at ambient temperature remained stable for up to 8 h of storage after which concentrations of both E1C and GCM changed significantly compared to controls extracted at time 0. Long-term storage in 80% MeOH at ambient temperature affected hormone concentrations significantly with concentrations of both E1C and GCM increasing after 6 and 4 months of storage, respectively. Drying fecal samples using a conventional oven at 50, 70, and 90 °C did not affect the E1C concentrations, but led to a significant decline for GCM concentrations in samples dried at 90 °C. Different fecal preservation techniques and extraction solvents provided similar results for both E1C and GCM concentrations. Our results confirm previous studies that prior to application of fecal hormone analysis in a new species, several preservation parameters should be evaluated for their effects on hormone metabolite stability. The results also provide several options for fecal preservation, extraction, and storage methods that can be selected depending on the condition of the field site and laboratory. |
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1610-7365 |
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no |
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Equine Behaviour @ team @ Gholib2018 |
Serial |
6521 |
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| Author |
Lee, P. |
| Title |
Adaptation to environmental change:an evolutionary perspective |
Type |
Book Chapter |
| Year |
1991 |
Publication |
Primate responses to environmental changes |
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Issue |
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Pages |
39-56 |
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Chapmann & Hall |
Place of Publication |
London |
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H. O. Box |
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Equine Behaviour @ team @ |
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6523 |
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Beck, B. B. |
| Title |
Animal tool behaviour: The use and manufacture of tools by animals |
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1980 |
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Garland |
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New York |
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Equine Behaviour @ team @ |
Serial |
6524 |
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Fisher, J.; Hinde, R. A. |
| Title |
The opening of milk bottles by birds |
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Journal Article |
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1994 |
Publication |
British Birds |
Abbreviated Journal |
British Birds |
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42 |
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347-357 |
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Equine Behaviour @ team @ |
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6525 |
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Kuczaj, S. A; Makecha, R.; Trone, M.; Paulos, R. D; Ramos, J. A. |
| Title |
Role of Peers in Cultural Innovation and Cultural Transmission: Evidence from the Play of Dolphin Calves |
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Journal Article |
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2006 |
Publication |
International Journal of Comparative Psychology |
Abbreviated Journal |
Int. J. Comp. Psychol |
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19 |
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2 |
Pages |
223-240 |
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Equine Behaviour @ team @ |
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6526 |
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| Author |
Thorpe, W. H. |
| Title |
Learning and Instinct in Animals. |
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1963 |
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Methuen |
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London |
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Equine Behaviour @ team @ |
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6527 |
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| Author |
Forrester, G.; Hudry, K.; Lindell, A.; Hopkins, W. D. |
| Title |
Cerebral Lateralization and Cognition: Evolutionary and Developmental Investigations of Behavioral Biases |
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2018 |
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238 |
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Academic Press |
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Cambridge |
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9780128146729 |
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no |
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Equine Behaviour @ team @ |
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6530 |
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