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Author (down) Kirkpatrick, J.F.; Shldeler, S.E.; Lasley, B.L.; Turner, J.W.J.
Title Pregnancy determination in uncaptured feral horses by means of fecal steroid conjugates Type Journal Article
Year 1991 Publication Theriogenology Abbreviated Journal Theriogenology
Volume 35 Issue 4 Pages 753-760
Keywords
Abstract This study was carried out to develop an accurate, rapid and inexpensive method for diagnosing pregnancy in uncaptured feral horses by analysis of fecal steroid metabolites and to compare the accuracy of this method with diagnosis by urinary estrone conjugates (E(1)C). Paired urine and fecal samples were collected from 40 sexually mature feral mares during August and October. Urine samples were extracted directly from the soil and analyzed by enzymeimmunoassay (EIA) for E(1)C. Water extracts of fecal samples were assayed by EIA for E(1)C and nonspecific progesterone metabolites (iPdG). Urinary E(1)C, fecal E(1)C and fecal iPdG concentrations for seven mares which produced foals were 3.9 +/- 1.3 (SEM) mug/mg creatinine, 4.2 +/- 0.8 ng/g feces and 1.411 +/- 569.6 ng/g feces, respectively. Urinary E(1)C and fecal E(1)C and iPdG concentrations for the 33 mares which did not produce foals were 0.1 +/- 0.0 mug/mg creatinine and 0.5 +/- 0.1 and 32.8 +/- 4.5 ng/g feces, respectively. These differed (P < 0.01) from values in mares which produced foals.
Address Department of Biological Sciences Eastern Montana College Billings, MT 59101 USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0093-691X ISBN Medium
Area Expedition Conference
Notes PMID:16726944 Approved no
Call Number refbase @ user @ Serial 146
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Author (down) Kirkpatrick, J.F.; Liu, I.M.; Turner, J.W.J.; Naugle, R.; Keiper, R.
Title Long-term effects of porcine zonae pellucidae immunocontraception on ovarian function in feral horses (Equus caballus) Type Journal Article
Year 1992 Publication Journal of reproduction and fertility Abbreviated Journal J Reprod Fertil
Volume 94 Issue 2 Pages 437-444
Keywords Animals; Contraception, Immunologic/*veterinary; *Egg Proteins; Estrogens, Conjugated (USP)/urine; Female; Glycoproteins/*pharmacology; Horses/immunology/*physiology; *Membrane Glycoproteins; Ovary/drug effects/*physiology; Progesterone/metabolism; *Receptors, Cell Surface; Swine/immunology; Time Factors; Zona Pellucida/*immunology
Abstract Ten feral mares free-roaming in Maryland, USA, were inoculated with porcine zonae pellucidae (PZP) protein before the breeding season for three consecutive years (1988-90). Ovarian function was monitored for 51 days during the peak of the breeding season after the third annual PZP inoculation, in seven of these mares and in four untreated control mares, by means of urinary oestrone conjugates and nonspecific progesterone metabolites. None of the ten inoculated mares became pregnant in 1990, compared with 55% of 20 control mares, which included two of the four monitored for ovarian function. Three of the untreated mares demonstrated apparent normal ovarian activity, characterized by preovulatory oestrogen peaks, concurrent progesterone nadirs at ovulation, breeding activity, and luteal-phase progesterone increases after ovulation. Two of the seven monitored PZP-treated mares demonstrated ovulatory cycles that did not result in conception. One was pregnant as a result of conception in 1989 and demonstrated a normal, late-gestation, endocrine profile. The remaining four PZP-treated mares revealed no evidence of ovulation, and urinary oestrogen concentrations were significantly depressed. The experiments indicated that (i) a third consecutive annual PZP booster inoculation is greater than 90% effective in preventing pregnancies in mares and (ii) three consecutive years of PZP treatment may interfere with normal ovarian function as shown by markedly depressed oestrogen secretion.
Address Deaconess Research Institute, Billings, MT 59102
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0022-4251 ISBN Medium
Area Expedition Conference
Notes PMID:1317449 Approved no
Call Number refbase @ user @ Serial 145
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Author (down) Kirkpatrick, J.F.; Turner, J.W. Jr
Title Comparative reproductive biology of North American feral horses Type Journal Article
Year 1986 Publication Journal of Equine Veterinary Science Abbreviated Journal J. Equine Vet. Sci.
Volume 6 Issue Pages 224-230
Keywords
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
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ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 2326
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