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Author  |
Flauger, B.; Krueger, K.; Gerhards, H.; Möstl, E. | ||||
| Title | Simplified method to measure glucocorticoid metabolites in faeces of horses | Type | Journal Article | ||
| Year | 2010 | Publication | Veterinary Research Communications | Abbreviated Journal | Vet Res Comm |
| Volume | 34 | Issue | 2 | Pages | 185-195 |
| Keywords | ACTH challenge; enzyme immunoassay; stress behaviour; cortisol | ||||
| Abstract | Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited racticability due to its complex extraction procedure. Therefore, we tested the applicability of other enzyme immunoassays for glucocorticoid metabolites. The present study clearly proved that an enzyme immunoassay (EIA) for 11-oxoetiocholanolone using 11-oxoetiocholanolone-17-CMO: BSA (3α,11-oxo-A EIA) as antigen showed high amounts of immunoreactive substances. Therefore it was possible to use just a small amount of the supernatant of a methanolic suspension of faeces. The results correlated well with the already described method for measuring GCMs in horse faeces, i.e. analysing the samples with an EIA after a two step clean up procedure of the samples (Merl et al. 2000). In addition, the 3α,11-oxo-A EIA has the advantage of providing a bigger difference between baseline values and peak values after ACTH stimulation. The new assay increased the accuracy of the test, lowered the expenses per sample, and storing samples at room temperature after collection was less critical than with other assays investigated in our study. This is a big advantage both in the field of wildlife management of equids and in the field of equestrian sports and it shows the importance of choosing an assay which is in good accordance with the metabolites excreted in a given species. |
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| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 5073 | ||
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| Author |
Flauger, B.; Krueger, K.; Gerhards, H.; Moestl, E. | ||||
| Title | Measurement of glucocorticoid metabolites in horse faeces: the validation of different group specific enzymeimmunoassays and extraction methods. | Type | Book Chapter | ||
| Year | 2009 | Publication | Proceeding of the 102. Annual meeting of the Deutsche Zoologische Gesellschaft (DZG). | Abbreviated Journal | |
| Volume | Issue | Pages | 166 | ||
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| Publisher | Dt. Zoologische Ges | Place of Publication | München | Editor | Cremer,S.;Schrempf,A.;Heinze,J. |
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| ISSN | ISBN | 978-3-00-028368-0 | Medium | ||
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| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 5718 | ||
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| Author |
Vollmerhaus, B.; Roos, H.; Gerhards, H.; Knospe, C. | ||||
| Title | [Phylogeny, form and function of canine teeth in the horse] | Type | Journal Article | ||
| Year | 2003 | Publication | Anatomia, histologia, embryologia | Abbreviated Journal | Anat Histol Embryol |
| Volume | 32 | Issue | 4 | Pages | 212-217 |
| Keywords | Animals; Cuspid/*anatomy & histology/radiography; Evolution; Horses/*anatomy & histology; Male; Phylogeny; *Social Dominance | ||||
| Abstract | The canine teeth of the horse developed phylogenically from the simple, pointed, short-rooted tooth form of the leaf eating, in pairs living, Eocene horse Hyracotherium and served up to the Oligocene as a means of defense (self preservation). In the Miocene the living conditions of the Merychippus changed and they took to eating grass and adopted as a new behavior the life in a herd. The canine teeth possibly played an important role in fights for social ranking; they changed from a crown form to knife-like shape. In the Pliohippus the canine tooth usually remained in male horses and since the Pliocene, it contributed to the fights between stallions, to ensure that the offspring only came from the strongest animals (preservation of the species). Form and construction of the canine tooth are described and discussed in detail under the above mentioned phylogenic and ethologic aspects. | ||||
| Address | Institut fur Tieranatomie und Chirurgische Tierklinik der Universitat Munchen, Veterinarstrasse 13, D 80539 Munchen, Deutschland. c-neumueller@anat.vetmed.uni-muenchen.de | ||||
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| Language | German | Summary Language | Original Title | [Zur Phylogenie, Form und Funktion der Dentes canini des Pferdes] | |
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| ISSN | 0340-2096 | ISBN | Medium | ||
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| Notes | PMID:12919071 | Approved | no | ||
| Call Number | refbase @ user @ | Serial | 672 | ||
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