Records |
Author |
Reynhout, I.C.; Cornelissen, J.J.L.M.; Nolte, R.J.M. |
Title |
Self-assembled architectures from biohybrid triblock copolymers |
Type |
Journal Article |
Year |
2007 |
Publication |
Journal of the American Chemical Society |
Abbreviated Journal |
J Am Chem Soc |
Volume |
129 |
Issue |
8 |
Pages |
2327-2332 |
Keywords |
Horseradish Peroxidase/*chemistry; Micelles; Molecular Structure; Myoglobin/*chemistry; Particle Size; Polyethylene Glycols/*chemistry; Polymers/*chemical synthesis/chemistry; Polystyrenes/*chemistry; Surface-Active Agents/chemical synthesis/chemistry |
Abstract |
The synthesis and self-assembly behavior of biohybrid ABC triblock copolymers consisting of a synthetic diblock, polystyrene-b-polyethylene glycol (PSm-b-PEG113), where m is varied, and a hemeprotein, myoglobin (Mb) or horse radish peroxidase (HRP), is described. The synthetic diblock copolymer is first functionalized with the heme cofactor and subsequently reconstituted with the apoprotein or the apoenzyme to yield the protein-containing ABC triblock copolymer. The obtained amphiphilic block copolymers self-assemble in aqueous solution into a large variety of aggregate structures. Depending on the protein and the polystyrene block length, micellar rods, vesicles, toroids, figure eight structures, octopus structures, and spheres with a lamellar surface are formed. |
Address |
Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands |
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English |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0002-7863 |
ISBN |
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Conference |
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Notes |
PMID:17274615 |
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no |
Call Number |
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Serial |
1832 |
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Author |
Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C. |
Title |
Molecular and cytogenetic paternity testing of a male offspring of a hinny |
Type |
Journal Article |
Year |
2006 |
Publication |
Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie |
Abbreviated Journal |
J Anim Breed Genet |
Volume |
123 |
Issue |
6 |
Pages |
403-405 |
Keywords |
Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal |
Abstract |
An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey. |
Address |
Equine Center, China Agricultural University, Beijing, China |
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Place of Publication |
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Language |
English |
Summary Language |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0931-2668 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:17177697 |
Approved |
no |
Call Number |
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Serial |
1846 |
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Author |
Villani, M.; Cairoli, F.; Kindahl, H.; Galeati, G.; Faustini, M.; Carluccio, A.; Veronesi, M.C. |
Title |
Effects of mating on plasma concentrations of testosterone, cortisol, oestrone sulphate and 15-ketodihydro-PGF2alpha in stallions |
Type |
Journal Article |
Year |
2006 |
Publication |
Reproduction in Domestic Animals = Zuchthygiene |
Abbreviated Journal |
Reprod Domest Anim |
Volume |
41 |
Issue |
6 |
Pages |
544-548 |
Keywords |
Animals; Dinoprost/*analogs & derivatives/blood; Ejaculation/physiology; Estrone/*analogs & derivatives/blood; Horses/*blood/physiology; Hydrocortisone/*blood; Male; Sexual Behavior, Animal/*physiology; Testosterone/*blood |
Abstract |
Very little information is available regarding the physiological mechanisms involved in the normal sexual activity in the stallion and, in particular, the endocrine control of reproduction is still not clearly understood. This experiment was designed to determine the short-term effect of sexual stimulation on plasma concentrations of testosterone, cortisol, oestrone sulphate and 15-ketodihydro-PGF(2alpha) in stallions. Semen samples were collected from 10 lighthorse stallions of proven fertility using a Missouri model artificial vagina. At the same time, blood samples were collected from the jugular vein with heparinized tubes, 20 and 10 min before oestrous mare exposure, at exposure and 10, 20, 30 min after dismounting. Testosterone concentrations showed a sharp rise 10 min after mating (p < 0.001), reached a plateau, and then showed a further increase 30 min after mating (p < 0.001). Cortisol concentrations increased 10 min after mating (p < 0.001) and remained at high levels in the subsequent samples taken. A peak of oestrone sulphate was observed 10 min after mating (p < 0.001). 15-Ketodihydro-PGF(2alpha) concentrations decreased rapidly at the moment of the exposure of the stallions to an oestrous mare (p < 0.05), returned to pre-mating concentrations and then decreased again 30 min after mating (p < 0.05). |
Address |
Dipartimento di Scienze Cliniche Veterinarie, Sez. Clinica Ostetrica e Ginecologica Veterinaria, Milan, Italy |
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English |
Summary Language |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0936-6768 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:17107515 |
Approved |
no |
Call Number |
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Serial |
1855 |
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Author |
Skedros, J.G.; Dayton, M.R.; Sybrowsky, C.L.; Bloebaum, R.D.; Bachus, K.N. |
Title |
The influence of collagen fiber orientation and other histocompositional characteristics on the mechanical properties of equine cortical bone |
Type |
Journal Article |
Year |
2006 |
Publication |
The Journal of Experimental Biology |
Abbreviated Journal |
J Exp Biol |
Volume |
209 |
Issue |
Pt 15 |
Pages |
3025-3042 |
Keywords |
Animals; Biomechanics; Bone and Bones/*physiology; Collagen/*physiology; Forelimb; Horses/*physiology |
Abstract |
This study examined relative influences of predominant collagen fiber orientation (CFO), mineralization (% ash), and other microstructural characteristics on the mechanical properties of equine cortical bone. Using strain-mode-specific (S-M-S) testing (compression testing of bone habitually loaded in compression; tension testing of bone habitually loaded in tension), the relative mechanical importance of CFO and other material characteristics were examined in equine third metacarpals (MC3s). This model was chosen since it had a consistent non-uniform strain distribution estimated by finite element analysis (FEA) near mid-diaphysis of a thoroughbred horse, net tension in the dorsal/lateral cortices and net compression in the palmar/medial cortices. Bone specimens from regions habitually loaded in tension or compression were: (1) tested to failure in both axial compression and tension in order to contrast S-M-S vs non-S-M-S behavior, and (2) analyzed for CFO, % ash, porosity, fractional area of secondary osteonal bone, osteon cross-sectional area, and population densities of secondary osteons and osteocyte lacunae. Multivariate multiple regression analyses revealed that in S-M-S compression testing, CFO most strongly influenced total energy (pre-yield elastic energy plus post-yield plastic energy); in S-M-S tension testing CFO most strongly influenced post-yield energy and total energy. CFO was less important in explaining S-M-S elastic modulus, and yield and ultimate stress. Therefore, in S-M-S loading CFO appears to be important in influencing energy absorption, whereas the other characteristics have a more dominant influence in elastic modulus, pre-yield behavior and strength. These data generally support the hypothesis that differentially affecting S-M-S energy absorption may be an important consequence of regional histocompositional heterogeneity in the equine MC3. Data inconsistent with the hypothesis, including the lack of highly longitudinal collagen in the dorsal-lateral ;tension' region, paradoxical histologic organization in some locations, and lack of significantly improved S-M-S properties in some locations, might reflect the absence of a similar habitual strain distribution in all bones. An alternative strain distribution based on in vivo strain measurements, without FEA, on non-Thoroughbreds showing net compression along the dorsal-palmar axis might be more characteristic of the habitual loading of some of the bones that we examined. In turn, some inconsistencies might also reflect the complex torsion/bending loading regime that the MC3 sustains when the animal undergoes a variety of gaits and activities, which may be representative of only a portion of our animals, again reflecting the possibility that not all of the bones examined had similar habitual loading histories. |
Address |
Utah Bone and Joint Center, 5323 S. Woodrow Street #202, Salt Lake City, UT 84107, USA. jskedros@utahboneandjoint.com |
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Place of Publication |
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English |
Summary Language |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0022-0949 |
ISBN |
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Area |
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Conference |
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Notes |
PMID:16857886 |
Approved |
no |
Call Number |
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Serial |
1868 |
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Author |
Candura, S.M.; Verni, P.; Minelli, C.M.; Rosso, G.L.; Cappelli, M.I.; Strambi, S.; Martellosio, V. |
Title |
[Occupational risks among public safety and security forces] |
Type |
Journal Article |
Year |
2006 |
Publication |
Giornale Italiano di Medicina del Lavoro ed Ergonomia |
Abbreviated Journal |
G Ital Med Lav Ergon |
Volume |
28 |
Issue |
1 |
Pages |
53-62 |
Keywords |
Burnout, Professional/etiology; Climate; Health Education; Humans; *Law Enforcement; Noise/adverse effects; *Occupational Diseases/chemically induced/etiology/prevention & control; *Occupational Health; *Police; Risk Assessment; Risk Factors; Stress Disorders, Post-Traumatic/etiology; Stress, Psychological/etiology; Vibration/adverse effects |
Abstract |
The present paper tries to identify the occupational risk factors (physical, chemical, biological, psychological), variable depending on jobs and tasks, to which the heterogeneous public safety/security workers are exposed. The fight against criminality and public order maintenance imply (sometimes fatal) traumatic risks, and expose to psychophysical and sensorial tiring, unfavourable macro- and microclimatic conditions, the risk of baropathy (air navigation, underwater activities), noise (generated by firearms and several other sources), vibrations and shakings (automatic weapons, transport vehicles), the risk of electric injury, ionizing (X and gamma rays) and non-inonizing (ultraviolet rays, microwaves and radiofrequencies, electromagnetic fields) radiations. Chemical hazards include carbon monoxide and other combustion products (fires, urban traffic), substances released in chemical accidents, tear gases, lead (firing grounds, metal works, environmental pollution), solvents, lubrificants and cutting oils (mechanic repair and maintenance), laboratory materials and reagents, irritant and/or sensitizing agents contained in gloves. The main biological risks are tetanus, blood-borne diseases (viral hepatitis, AIDS), aerogenous diseases (e.g., tuberculosis, Legionnaire's disease, epidemic cerebrospinal meningitis), dog- or horse-transmitted zoonosis. Finally, emotional, psychosomatic and behavioural stress-related disorders (e.g., burn-out syndrome, post-traumatic stress disorder) are typically frequent. The presence of numerous and diversified hazards among public safety/security forces imposes the adoption of occupational medicine measures, including risk assessment, health education, technical and environmental prevention, personal protective devices, sanitary surveillance and biological monitoring, clinical interventions (diagnosis, therapy and rehabilitation of occupational accidents and illnesses), prompt medico-legal evaluation of occupational-related compensation claims. |
Address |
Scuola di Specializzazione in Medicina del Lavoro, Universita degli Studi di Pavia, Italy |
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Place of Publication |
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Language |
Italian |
Summary Language |
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Original Title |
Rischi professionali nelle Forze dell'Ordine |
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Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
1592-7830 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:16705889 |
Approved |
no |
Call Number |
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Serial |
1871 |
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Author |
Ionita, J.C.; Poncet, P.A.; Doherr, M.G.; Steiger, A. |
Title |
[Evaluation of the quality of husbandry of Franches-Montagnes horses in their breeding farms] |
Type |
Journal Article |
Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
Volume |
148 |
Issue |
4 |
Pages |
191-197 |
Keywords |
Animal Husbandry/methods/*standards/statistics & numerical data; *Animal Welfare; Animals; Breeding/*methods/standards; Female; Floors and Floorcoverings; Horses/*physiology; Housing, Animal/*standards; Male; Poaceae; Questionnaires; Social Behavior; Switzerland |
Abstract |
The quality of husbandry of Franches-Montagnes horses (FM) in Switzerland is evaluated on the basis of an investigation carried out in 2002 by the Swiss FM breeding federation. Questionnaires were sent to 3500 of its members and the results include data from 968 breeding enterprises, housing a total of 3965 FM: 46.1% were breeding mares (61.0% with foal at foot), 26.5% young stock, 1.3% stallions and 26.0% non breeding stock (74.6% of which were pleasure horses and 25.4% working horses). 57.6% of the FM were housed in individual boxes with or without permanent outdoor access, 25.4% were hold in groups with or without permanent outdoor access, the remaining 17.0% were kept in standing stalls. 95.0% of the FM had at least visual contact with other equines and 99.2% had sufficient light in their stable. 88.1% were stabled on long stalk straw, while only 4.3% were bedded on other materials other than straw. The average time spent at pasture per horse and per week ranged from 96.5 +/- 51.6 hours in summer to 27.2 +/- 26.7 hours in winter. On average, a FM is used for 8.3 +/- 6.5 hours per week. Horses with an paddock at their disposal spend an average of 39.8 +/- 45.9 hours there per week. |
Address |
Chirurgische Tierklinik, Universitat Leipzig |
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Language |
French |
Summary Language |
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Original Title |
Les conditions de detention des chevaux de race franches-montagnes dans leurs exploitations d'elevage |
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Edition |
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ISSN |
0036-7281 |
ISBN |
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Notes |
PMID:16703760 |
Approved |
no |
Call Number |
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Serial |
1872 |
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Author |
Poncet, P.A.; Ionita, J.C.; Doherr, M.G.; Steiger, A. |
Title |
[The influence of the socio-economic structure of the breeding farms of Franches-Montagnes horses on the conditions of husbandry] |
Type |
Journal Article |
Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
Volume |
148 |
Issue |
4 |
Pages |
183-189 |
Keywords |
Altitude; Animal Husbandry/methods/*standards; Animals; Breeding/*economics/standards; Demography; Female; Horses/*physiology; Housing, Animal/*standards; Male; Physical Conditioning, Animal/methods/*physiology; Questionnaires; Social Behavior; Socioeconomic Factors; Switzerland |
Abstract |
The socio-economic structure of the breeding farms of Franches-Montagnes horses (FM) in Switzerland is evaluated on the basis of an investigation carried out in 2002 by the Swiss FM breeding federation. Questionnaires were sent to 3500 of its members and the results include data from 968 breeding enterprises, housing a total of 3965 FM. The quality of the husbandry of FM varies according to factors such as the altitude and the geographical situation of the farms and studs. Socio-economic parameters, such as the role of FM in the business, their use (breeding, driving, riding) and the age and level of professional education of the owners may also have an effect on standards of husbandry. The results show that the owners for whom FM represent a source of income more frequently keep their horses in standing stalls, but give them more time to exercise at liberty than the horses belonging to amateur breeders. Younger and better educated breeders are more likely to house their animals in groups. |
Address |
Haras national suisse, Avenches |
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Language |
French |
Summary Language |
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Original Title |
L'influence de la structure socio-economique des exploitations d'elevage de chevaux de race franches-montagnes sur les conditions de detention |
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Edition |
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ISSN |
0036-7281 |
ISBN |
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Notes |
PMID:16703759 |
Approved |
no |
Call Number |
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Serial |
1873 |
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Author |
Christie, J.L.; Hewson, C.J.; Riley, C.B.; McNiven, M.A.; Dohoo, I.R.; Bate, L.A. |
Title |
Management factors affecting stereotypies and body condition score in nonracing horses in Prince Edward Island |
Type |
Journal Article |
Year |
2006 |
Publication |
The Canadian Veterinary Journal. La Revue Veterinaire Canadienne |
Abbreviated Journal |
Can Vet J |
Volume |
47 |
Issue |
2 |
Pages |
136-143 |
Keywords |
*Animal Husbandry/standards/statistics & numerical data; *Animal Welfare; Animals; Behavior, Animal/*physiology; Body Constitution/*physiology; Female; Horses/*physiology; Male; Prince Edward Island; Questionnaires; Risk Factors; Sex Factors |
Abstract |
In North America, there are few representative data about the effects of management practices on equine welfare. In a randomized survey of 312 nonracing horses in Prince Edward Island (response rate 68.4%), owners completed a pretested questionnaire and a veterinarian examined each horse. Regression analyses identified factors affecting 2 welfare markers: body condition score (BCS) and stereotypic behavior. Horses' BCSs were high (mean 5.7, on a 9-point scale) and were associated with sex (males had lower BCSs than females; P < 0.001) and examination date (P = 0.052). Prevalences of crib biting, wind sucking, and weaving were 3.8%, 3.8%, and 4.8%, respectively. Age (OR = 1.07, P = 0.08) and hours worked weekly (OR = 1.12, P = 0.03) were risk factors for weaving. Straw bedding (OR = 0.3, P = 0.03), daily hours at pasture (OR = 0.94, P = 0.02), and horse type (drafts and miniatures had a lower risk than light horses; P = 0.12) reduced the risk of horses showing oral stereotypies. Some of these results contradict those of other studies perhaps because of populations concerned. |
Address |
Sir James Dunn Animal Welfare Centre, Department of Biomedical Sciences, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island |
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English |
Summary Language |
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Series Editor |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0008-5286 |
ISBN |
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Medium |
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Area |
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Conference |
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Notes |
PMID:16579039 |
Approved |
no |
Call Number |
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Serial |
1875 |
Permanent link to this record |
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Author |
Lin, Y.-L.; Moolenaar, H.; van Weeren, P.R.; van de Lest, C.H.A. |
Title |
Effect of microcurrent electrical tissue stimulation on equine tenocytes in culture |
Type |
Journal Article |
Year |
2006 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
Volume |
67 |
Issue |
2 |
Pages |
271-276 |
Keywords |
Animals; Apoptosis; Cell Proliferation; Cells, Cultured; Electric Stimulation; *Horses; Tendons/*cytology |
Abstract |
OBJECTIVE: To determine effects of microcurrent electrical tissue stimulation (METS) on equine tenocytes cultured from the superficial digital flexor tendon (SDFT). SAMPLE POPULATION: SDFTs were collected from 20 horses at slaughter. PROCEDURE: Tenocytes were isolated following outgrowth from explants and grown in 48-well plates. Four methods of delivering current to the tenocytes with a METS device were tested. Once the optimal method was selected, current consisting of 0 (negative control), 0.05, 0.1, 0.5, 1.0, or 1.5 mA was applied to cells (8 wells/current intensity) once daily for 8 minutes. Cells were treated for 1, 2, or 3 days. Cell proliferation, DNA content, protein content, and apoptosis rate were determined. RESULTS: Application of microcurrent of moderate intensity increased cell proliferation and DNA content, with greater increases with multiple versus single application. Application of microcurrent of moderate intensity once or twice increased protein content, but application 3 times decreased protein content. Application of current a single time did not significantly alter apoptosis rate; however, application twice or 3 times resulted in significant increases in apoptosis rate, and there were significant linear (second order) correlations between current intensity and apoptosis rate when current was applied twice or 3 times. CONCLUSIONS AND CLINICAL RELEVANCE: Results of the present study indicate that microcurrent affects the behavior of equine tenocytes in culture, but that effects may be negative or positive depending on current intensity and number of applications. Therefore, results are far from conclusive with respect to the suitability of using METS to promote tendon healing in horses. |
Address |
Department of Equine Sciences, Faculty of Veterinary Medicine, State University of Utrecht, Utrecht, The Netherlands |
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English |
Summary Language |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0002-9645 |
ISBN |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:16454632 |
Approved |
no |
Call Number |
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Serial |
1878 |
Permanent link to this record |
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Author |
Kaiser, L.; Heleski, C.R.; Siegford, J.; Smith, K.A. |
Title |
Stress-related behaviors among horses used in a therapeutic riding program |
Type |
Journal Article |
Year |
2006 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
Volume |
228 |
Issue |
1 |
Pages |
39-45 |
Keywords |
Adult; Animals; Behavior, Animal/*physiology; Child; *Disabled Persons; Female; Horses/*psychology; Humans; Male; *Mentally Disabled Persons; Occupational Therapy/methods; Recreation; Risk Factors; Stress/*veterinary |
Abstract |
OBJECTIVE: To determine whether therapeutic riding resulted in higher levels of stress or frustration for horses than did recreational riding and whether therapeutic riding with at-risk individuals was more stressful for the horses than was therapeutic riding with individuals with physical or emotional handicaps. DESIGN: Observational study. ANIMALS: 14 horses in a therapeutic riding program. PROCEDURE: An ethogram of equine behaviors was created, and horses were observed while ridden by 5 groups of riders (recreational riders, physically handicapped riders, psychologically handicapped riders, at risk children, and special education children). Number of stress-related behaviors (ears pinned back, head raised, head turned, head tossed, head shaken, head down, and defecation) was compared among groups. RESULTS: No significant differences in mean number of stress-related behaviors were found when horses were ridden by recreational riders, physically handicapped riders, psychologically handicapped riders, or special education children. However, mean number of stress-related behaviors was significantly higher when horses were ridden by the at-risk children. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggest that for horses in a therapeutic riding program, being ridden by physically or psychologically handicapped individuals is no more stressful for the horses than is being ridden in the same setting by recreational riders. However, at-risk children caused more stress to the horses, suggesting that the time horses are ridden by at-risk children should be limited both daily and weekly. |
Address |
Human-Animal Bond Initiative, College of Nursing, Michigan State University, East Lansing, MI 48824, USA |
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Place of Publication |
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English |
Summary Language |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0003-1488 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:16426164 |
Approved |
no |
Call Number |
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Serial |
1879 |
Permanent link to this record |