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Cerasoli, F.; Podaliri Vulpiani, M.; Saluti, G.; Conte, A.; Ricci, M.; Savini, G.; D'Alterio, N. |
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Title |
Assessment of Welfare in Groups of Horses with Different Management, Environments and Activities by Measuring Cortisol in Horsehair, Using Liquid Chromatography Coupled to Hybrid Orbitrap High-Resolution Mass Spectrometry |
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2022 |
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Animals |
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Animals |
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12 |
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14 |
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Keywords |
cortisol; animal welfare; horse; Lc-Hrms/Ms |
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Abstract |
Horses have always been animals used for companionship, work, transportation, and performance purposes over the history of humanity; there are different ways of managing horses, but studies on how horse welfare is influenced by different activities and managements are scanty. Understanding how the management, the environment, and the different uses of horses can affect the level of stress and well-being is important not only for people associated with horses. Three groups of horses with different management, environments, and activities were selected: (1) stabled horses ridden frequently, (2) horses that perform public order service under the Italian state police, and (3) free-ranging horses. Cortisol analysis was carried out on horsehair samples using liquid chromatography coupled to hybrid orbitrap high-resolution mass spectrometry (LC-HRMS/MS), a laboratory technique used for the first time to quantify horsehair cortisol. The selection of horses to be included in the three groups was carried out by including only subjects with positive welfare assessment in accordance with the horse welfare assessment protocol (AWIN). These analyses demonstrated that the cortisol levels detected in the horsehair of free-ranging animals were significantly higher compared to those detected in stabled and working horses. These results may have been a consequence of complex environmental, managerial, and behavioral factors, which should be worth further investigation |
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Animals |
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12 |
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14 |
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2076-2615 |
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Equine Behaviour @ team @ |
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6674 |
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Epstein H, |
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Title |
Descent and origin of the ass |
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Book Chapter |
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1971 |
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In Epstein: The origin of the domestic animals of Africa. II. |
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394-398 |
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from Professor Hans Klingels Equine Reference List |
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no |
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1069 |
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Klingel H, |
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Title |
Communication in Perissodactyla |
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1977 |
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in Sebeok TA (ed): How Animals Communicate |
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715-727, |
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from Professor Hans Klingels Equine Reference List |
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1305 |
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Rossdale Pd, |
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Title |
Perinatal behaviour int the thoroughbred horse |
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1968 |
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In: Fox,M.W. (ed.): Abnormal behavior in Animals. |
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Chapt. 15 |
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from Professor Hans Klingels Equine Reference List |
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no |
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1517 |
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WARING GH et al, |
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Title |
The behaviour of horses |
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In: Behaviour of domestic animals |
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330-369 |
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from Professor Hans Klingels Equine Reference List |
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no |
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1698 |
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Bannikov, A.G. |
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Title |
The Asiatic Wild Ass: neglected relative of the horse |
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Journal Article |
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1971 |
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Animals |
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Animals |
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13 |
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580-585 |
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Englisch |
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refbase @ user @ |
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756 |
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Berliner Vr, |
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The estrous cycle of the mare. In: Cole,H.H., Cupps,P.T. |
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1959 |
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Reproductions in domestic animals |
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1 |
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267-289 |
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from Professor Hans Klingels Equine Reference List |
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no |
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942 |
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Bruemmer F, |
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Title |
The horses of Sable Island |
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Journal Article |
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1967 |
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Animals |
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10 |
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14-17 |
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from Professor Hans Klingels Equine Reference List |
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975 |
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Author |
Epstein H, |
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Title |
Wild Ass |
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Journal Article |
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1971 |
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In Epstein: The origin of the domestic animals of Africa. II |
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378-381 |
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from Professor Hans Klingels Equine Reference List |
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1070 |
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Author |
Breummer, F |
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Title |
The wild horses of Sable Island |
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1967 |
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Animals |
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Animals |
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10 |
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14-17 |
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Equine Behaviour @ team @ |
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2248 |
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