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Palme, R.; Touma, C.; Arias,N.; Dominchin, M.F.; Lepschy, M. |
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Title |
Steroid extraction: Get the best out of faecal samples |
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Year  |
2012 |
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Veterinary Medicine Austria |
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Vet. Med. Austria |
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100 |
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238-246 |
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Abstract |
Faecal steroid hormone metabolites are becoming increasingly popular as parameters for reproductive functions and stress. Theextraction of the steroids from the faecal matrix represents the initial step before quantification can be performed. The steroid metabolites present in the faecal matrix are of varying polarity and composition, so selection of a proper extraction procedure is essential. There have been some studies to address this complex but often neglected point. Radiolabelled
steroids (e.g. cortisol or progesterone) have frequently been added to faecal samples to estimate the efficiency of the extraction procedures used. However, native, unmetabolized steroids are normally not present in the faeces and therefore the results are artificial and do not accurately reflect the actual recoveries of the substances of interest. In this respect, recovery experiments based on faecal samples from radiometabolism studies are more informative. In these samples, the metabolite content accurately reflects the mixture of metabolites present in the given species. As a result, it is possible to evaluate different extraction methods for use with faecal samples. We present studies on sheep, horses, pigs, hares and dogs that utilized samples containing naturally metabolized, 14C-labelled steroids. |
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Review, faeces, extrac- tion, non-invasive hormone moni- toring, stress, reproduction. |
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Equine Behaviour @ team @ |
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6046 |
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Author |
Heyes, C. |
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Title |
What's social about social learning? |
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2012 |
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J Comp Psychol |
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120 |
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Equine Behaviour @ team @ Heyes2012 |
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6228 |
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Smaers, J.B.; Dechmann, D.K.N.; Goswami, A.; Soligo, C.; Safi, K. |
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Comparative analyses of evolutionary rates reveal different pathways to encephalization in bats, carnivorans, and primates |
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2012 |
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Proc Natl Acad Sci U S A |
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109 |
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Equine Behaviour @ team @ Smaers2012 |
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6238 |
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Author |
Benson-Amram, S.; Holekamp, K.E. |
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Title |
Innovative problem solving by wild spotted hyenas |
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2012 |
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Proc R Soc B |
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Proc R Soc B |
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279 |
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4087-4095 |
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Equine Behaviour @ team @ Benson-Amram2012 |
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6266 |
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Author |
Thornton, A.; Samson, J. |
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Title |
Innovative problem solving in wild meerkats |
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2012 |
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Anim Behav |
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83 |
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Equine Behaviour @ team @ Thornton2012 |
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6267 |
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Author |
Briefer, E.F.; Padilla de la Torre, M.; McElligott, A.G. |
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Title |
Mother goats do not forget their kids' calls |
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2012 |
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Proc R Soc B |
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279 |
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Equine Behaviour @ team @ Briefer2012 |
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6282 |
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Author |
Van Horik, J.; Clayton, N.; Emery, N. |
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Title |
Convergent evolution of cognition in Corvids, Apes and other animals |
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2012 |
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Oxford Handbook of Comparative Evolutionary Psychology |
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Oxford University Press |
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New York |
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Vonk, J.; Shackelford, T. |
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Equine Behaviour @ team @ Van Horik2012 |
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6284 |
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Author |
Tennie, C.; Call, J.; Tomasello, M. |
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Untrained chimpanzees (Pan troglodytes schweinfurthii) fail to imitate novel actions |
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2012 |
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PLoS One |
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7 |
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Equine Behaviour @ team @ Tennie2012 |
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6289 |
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Author |
Van Schaik, C.P.; Isler, K.; Burkart, J.M. |
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Title |
Explaining brain size variation: from social to cultural brain |
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2012 |
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Trends Ecol Evol |
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16 |
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Equine Behaviour @ team @ Van Schaik2012 |
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6304 |
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Author |
Range, F.; Möslinger, H.; Virányi, Z. |
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Title |
Domestication has not affected the understanding of means-end connections in dogs |
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2012 |
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Anim Cogn |
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15 |
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Equine Behaviour @ team @ Range2012 |
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6322 |
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