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Author Vetvik, H.; Grewal, H.M.S.; Haugen, I.L.; Åhrén, C.; Haneberg, B.
Title Mucosal antibodies can be measured in air-dried samples of saliva and feces Type Journal Article
Year (down) 1998 Publication Journal of Immunological Methods Abbreviated Journal
Volume 215 Issue 1–2 Pages 163-172
Keywords Saliva; Feces; IgA; IgG; Air-drying
Abstract IgA antibodies reflecting airways or intestinal mucosal immune responses can be found in saliva and feces, respectively, and IgG antibodies reflecting serum antibodies can be found in saliva. In this study, antibodies were detected in samples of saliva and feces which had been air-dried at room temperature (+20°C) or +37°C, and stored at these temperatures for up to 6 months. In saliva the antibody levels increased, while the antibodies in feces decreased upon storage. The individual IgA antibody concentrations which were adjusted by using the ratios of specific IgA/total IgA were relatively stable in both saliva and feces, and correlated with corresponding antibody levels in samples which had been stored at -20°C. The results indicate that air-dried saliva and feces can be used for semiquantitative measurements of mucosal antibodies, even after prolonged storage at high temperatures and lack of refrigeration.
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ISSN 0022-1759 ISBN Medium
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5996
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Author Skandakumar, S.; Stodulski, G.; Hau, J.
Title Salivary IgA: a Possible Stress Marker In Dogs Type Abstract
Year (down) 1995 Publication Animal Welfare Abbreviated Journal
Volume 4 Issue 4 Pages 339-350
Keywords Animal Welfare; Behaviour; Cortisol; Dog; Salivary Iga (S-Iga); Stress; Well-Being
Abstract Stress in humans has been reported to be associated with a decrease in the salivary immunoglobulin A (s-IgA) levels enabling the possible use of s-IgA to assess stress. Prolonged stress, if reliably assessed in a non-invasive manner, may be used to assess animal welfare. This study analysed groups of dogs undergoing physical and temperamental training and s-IgA levels were measured by rocket immunoelectrophoresis in prospective samples. Behavioural assessment was carried out and cortisol levels in saliva were measured by ELISA. A significant negative correlation (P < 0.007) between the logarithmic cortisol concentrations and s-IgA levels in saliva was recorded. The behavioural assessment of the dogs agreed well with the biochemical markers. It is concluded that IgA levels in saliva may be a useful marker of dog well-being and that stress results in decreased s-IgA levels.
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Call Number Equine Behaviour @ team @ Serial 5964
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Author Stahl, F.; Dorner, G.
Title Responses of salivary cortisol levels to stress-situations Type Journal Article
Year (down) 1982 Publication Endokrinologie Abbreviated Journal Endokrinologie
Volume 80 Issue 2 Pages 158-162
Keywords Adrenocorticotropic Hormone/diagnostic use; Anxiety Disorders/metabolism; Circadian Rhythm; Cushing Syndrome/metabolism; Fear/physiology; Female; Humans; Hydrocortisone/*metabolism; Male; Pain/metabolism; Pregnancy; Saliva/*metabolism; Stress/*metabolism
Abstract A procedure is described for determining salivary cortisol levels by a competitive protein-binding assay using horse transcortin. The collection of saliva was performed by means of filter paper-strips. Filter paper samples are more than 5 days stable after air-drying. In this form, the samples could be stored without refrigerator or deep-freezer and, if necessary, sent by post to the laboratory without any special precaution. Stressful situation of either painful or anxious origin were associated with an adequate increase of salivary cortisol levels. The increases were 157 to 230% of the initial or normal values dependent on the kind of stress. The mean values in 4 cases of Cushing's syndrome were 380% and 1 hour after 25 I.U. ACTH 690% higher than those in normal persons. In normal persons, a well-defined circadian rhythm has been observed.
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ISSN 0013-7251 ISBN Medium
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Notes PMID:6297880 Approved no
Call Number refbase @ user @ Serial 4056
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Author Alexander, F.; Nicholson, J.D.
Title The blood and saliva clearances of phenobarbitone and pentobarbitone in the horse Type Journal Article
Year (down) 1968 Publication Biochemical pharmacology Abbreviated Journal Biochem Pharmacol
Volume 17 Issue 2 Pages 203-210
Keywords Animals; Female; *Horses; Injections, Intravenous; Male; Metabolic Clearance Rate; Pentobarbital/blood/*metabolism; Phenobarbital/blood/*metabolism; Protein Binding; *Saliva; Time Factors
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ISSN 0006-2952 ISBN Medium
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Notes PMID:5647047 Approved no
Call Number refbase @ user @ Serial 117
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Author Alexander, F.; Horner, M.W.; Moss, M.S.
Title The salivary secretion and clearance in the horse of chloral hydrate and its metabolites Type Journal Article
Year (down) 1967 Publication Biochemical pharmacology Abbreviated Journal Biochem Pharmacol
Volume 16 Issue 7 Pages 1305-1311
Keywords Animals; Chloral Hydrate/blood/*metabolism/urine; Chromatography, Gas; Ethanol/blood/urine; Horses; Male; Parotid Gland/metabolism; Saliva/*analysis; Trichloroacetic Acid/blood/urine
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ISSN 0006-2952 ISBN Medium
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Notes PMID:6053598 Approved no
Call Number refbase @ user @ Serial 118
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Author Alexander, F.
Title A study of parotid salivation in the horse Type Journal Article
Year (down) 1966 Publication The Journal of physiology Abbreviated Journal J Physiol
Volume 184 Issue 3 Pages 646-656
Keywords Animals; Atropine/*pharmacology; Bicarbonates/metabolism; Calcium/metabolism; Chlorides/metabolism; Horses; Mastication/*physiology; Parotid Gland/*physiology; Pilocarpine/*pharmacology; Potassium/metabolism; Salivation/*drug effects; Sodium/metabolism; Tetracaine/*pharmacology
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Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
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ISSN 0022-3751 ISBN Medium
Area Expedition Conference
Notes PMID:5963737 Approved no
Call Number refbase @ user @ Serial 119
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