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Author |
Momozawa, Y.; Takeuchi, Y.; Tozaki, T.; Kikusui, T.; Hasegawa, T.; Raudsepp, T.; Chowdhary, B.P.; Kusunose, R.; Mori, Y. |
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Title |
SNP detection and radiation hybrid mapping in horses of nine candidate genes for temperament |
Type |
Journal Article |
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Year |
2007 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
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Volume |
38 |
Issue |
1 |
Pages |
81-83 |
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Keywords |
Animals; *Behavior, Animal; Breeding; Horses/*genetics/physiology; *Polymorphism, Single Nucleotide; Radiation Hybrid Mapping; *Temperament |
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Laboratory of Veterinary Ethology, The University of Tokyo, Tokyo 113-8657, Japan |
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English |
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0268-9146 |
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PMID:17257195 |
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no |
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Call Number |
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Serial |
1834 |
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Author |
Bannasch, D.; Rinaldo, C.; Millon, L.; Latson, K.; Spangler, T.; Hubberty, S.; Galuppo, L.; Lowenstine, L. |
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Title |
SRY negative 64,XX intersex phenotype in an American saddlebred horse |
Type |
Journal Article |
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Year |
2007 |
Publication |
Veterinary Journal (London, England : 1997) |
Abbreviated Journal |
Vet J |
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Volume |
173 |
Issue |
2 |
Pages |
437-439 |
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Keywords |
Animals; Female; Genitalia/abnormalities; Hermaphroditism/*veterinary; Horse Diseases/*diagnosis/genetics; Horses/*genetics/*physiology; Karyotyping; Phenotype; Sex Differentiation; Sex Differentiation Disorders/diagnosis/veterinary; Sex-Determining Region Y Protein/genetics/*metabolism |
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Abstract |
A female American saddlebred horse was presented for surgical correction of a possible pseudohermaphrodite condition. The horse had abnormal external genitalia and exhibited stallion-like behaviour. No evidence of uterine or ovarian tissue was identified on laparoscopic examination, but hypoplastic testicular-like tissue was removed, although this was found to contain no spermatogonia upon histopathological examination. A karyotype was performed and showed the normal chromosomal complement for a female horse (64,XX). Polymerase chain reaction to detect the SRY gene was negative in peripheral blood as well as the testicular-like tissue. This case represents the first report of an SRY negative XX-male sex reversal intersex phenotype, which is a potentially inherited condition, in an American saddlebred horse. |
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Address |
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. dlbannasch@ucdavis.edu |
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1090-0233 |
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PMID:16386440 |
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no |
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Call Number |
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Serial |
1882 |
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Author |
Crosby, M.B.; Zhang, J.; Nowling, T.M.; Svenson, J.L.; Nicol, C.J.; Gonzalez, F.J.; Gilkeson, G.S. |
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Title |
Inflammatory modulation of PPAR gamma expression and activity |
Type |
Journal Article |
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Year |
2006 |
Publication |
Clinical immunology |
Abbreviated Journal |
Clin Immunol |
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Volume |
118 |
Issue |
2-3 |
Pages |
276-283 |
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Keywords |
Age Factors; Animals; Cell Line, Transformed; Cells, Cultured; Female; Inflammation Mediators/*physiology; Kidney/metabolism; Mesangial Cells/metabolism; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred MRL lpr; Mice, Knockout; Nitric Oxide/biosynthesis; Nitric Oxide Synthase Type II/biosynthesis/genetics; PPAR gamma/*biosynthesis/*genetics/metabolism; Up-Regulation/immunology |
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Abstract |
Nitric oxide (NO) production increases with age in the lupus-prone MRL/lpr mouse, paralleling disease activity. One mechanism for excess NO production in MRL/lpr mice may be a defect in down-regulatory mechanisms of the iNOS pathway. A potential modulator of NO is the nuclear hormone receptor peroxisome proliferation activated receptor gamma (PPARgamma). We demonstrate that renal PPARgamma protein expression was altered as disease progressed in MRL/lpr mice, which paralleled increased iNOS protein expression. Additionally, MRL/lpr-derived primary mesangial cells expressed less PPARgamma than BALB/c mesangial cells and produced more NO in response to LPS and IFNgamma. Furthermore, PPARgamma activity was reduced in mesangial cells following exposure to inflammatory mediators. This activity was restored with the addition of a NOS enzyme inhibitor. These results indicate that the activation of inflammatory pathways may lead to reduced activity and expression of PPARgamma, further exacerbating the disease state. |
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Address |
Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA |
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English |
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ISSN |
1521-6616 |
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Notes |
PMID:16303334 |
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no |
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Call Number |
refbase @ user @ |
Serial |
67 |
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Author |
Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C. |
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Title |
Molecular and cytogenetic paternity testing of a male offspring of a hinny |
Type |
Journal Article |
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Year |
2006 |
Publication |
Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie |
Abbreviated Journal |
J Anim Breed Genet |
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Volume |
123 |
Issue |
6 |
Pages |
403-405 |
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Keywords |
Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal |
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Abstract |
An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey. |
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Address |
Equine Center, China Agricultural University, Beijing, China |
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ISSN |
0931-2668 |
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Notes |
PMID:17177697 |
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no |
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Call Number |
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Serial |
1846 |
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Author |
Nettle, D. |
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Title |
The evolution of personality variation in humans and other animals |
Type |
Journal Article |
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Year |
2006 |
Publication |
The American Psychologist |
Abbreviated Journal |
Am Psychol |
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Volume |
61 |
Issue |
6 |
Pages |
622-631 |
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Keywords |
Animals; Birds; *Evolution; Female; Fishes; Humans; Insects; Male; Personality/*genetics/*physiology |
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Abstract |
A comprehensive evolutionary framework for understanding the maintenance of heritable behavioral variation in humans is yet to be developed. Some evolutionary psychologists have argued that heritable variation will not be found in important, fitness-relevant characteristics because of the winnowing effect of natural selection. This article propounds the opposite view. Heritable variation is ubiquitous in all species, and there are a number of frameworks for understanding its persistence. The author argues that each of the Big Five dimensions of human personality can be seen as the result of a trade-off between different fitness costs and benefits. As there is no unconditionally optimal value of these trade-offs, it is to be expected that genetic diversity will be retained in the population. |
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Address |
University of Newcastle, Newcastle, United Kingdom. daniel.nettle@ncl.ac.uk |
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English |
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0003-066X |
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Notes |
PMID:16953749 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
4105 |
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Permanent link to this record |
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Author |
Edwards, D.H.; Spitzer, N. |
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Title |
6. Social dominance and serotonin receptor genes in crayfish |
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Journal Article |
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Year |
2006 |
Publication |
Current Topics in Developmental Biology |
Abbreviated Journal |
Curr Top Dev Biol |
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Volume |
74 |
Issue |
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Pages |
177-199 |
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Keywords |
Animals; Astacoidea/*genetics/physiology; Humans; Receptors, Serotonin/*genetics; Serotonin/physiology; *Social Dominance |
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Abstract |
Gene expression affects social behavior only through changes in the excitabilities of neural circuits that govern the release of the relevant motor programs. In turn, social behavior affects gene expression only through patterns of sensory stimulation that produce significant activation of relevant portions of the nervous system. In crayfish, social interactions between pairs of animals lead to changes in behavior that mark the formation of a dominance hierarchy. Those changes in behavior result from changes in the excitability of specific neural circuits. In the new subordinate, circuits for offensive behavior become less excitable and those for defensive behavior become more excitable. Serotonin, which is implicated in mechanisms for social dominance in many animals, modulates circuits for escape and avoidance responses in crayfish. The modulatory effects of serotonin on the escape circuits have been found to change with social dominance, becoming excitatory in dominant crayfish and inhibitory in subordinates. These changes in serotonin's effects on escape affect the synaptic response to sensory input of a single cell, the lateral giant (LG) command neuron for escape. Moreover, these changes occur over a 2-week period and for the subordinate are reversible at any time following a reversal of the animal's status. The results have suggested that a persistent change in social status leads to a gradual change in the expression of serotonin receptors to a pattern that is more appropriate for the new status. To test that hypothesis, the expression patterns of crayfish serotonin receptors must be compared in dominant and subordinate animals. Two of potentially five serotonin receptors in crayfish have been cloned, sequenced, and pharmacologically characterized. Measurements of receptor expression in the whole CNS of dominant and subordinate crayfish have produced inconclusive results, probably because each receptor is widespread in the nervous system and is likely to experience opposite expression changes in different areas of the CNS. Both receptors have recently been found in identified neurons that mediate escape responses, and so the next step will be to measure their expression in these identified cells in dominant and subordinate animals. |
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Address |
Department of Biology, Georgia State University, Atlanta, GA 30302, USA |
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English |
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ISSN |
0070-2153 |
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Notes |
PMID:16860668 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
4364 |
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Permanent link to this record |
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Author |
Traversa, D.; Otranto, D.; Iorio, R.; Giangaspero, A. |
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Title |
Molecular characterization of Thelazia lacrymalis (Nematoda, Spirurida) affecting equids: a tool for vector identification |
Type |
Journal Article |
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Year |
2005 |
Publication |
Molecular and Cellular Probes |
Abbreviated Journal |
Mol Cell Probes |
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Volume |
19 |
Issue |
4 |
Pages |
245-249 |
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Keywords |
Animals; Horse Diseases/parasitology; Horses/*parasitology; Insect Vectors/*parasitology; Muscidae/*parasitology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Spirurida Infections/parasitology/veterinary; Thelazioidea/chemistry/*genetics |
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Abstract |
Equine thelaziosis caused by the eyeworm Thelazia lacrymalis is a parasitic disease transmitted by muscid flies. Although equine thelaziosis is known to have worldwide distribution, information on the epidemiology and presence of the intermediate hosts of T. lacrymalis is lacking. In the present work, a PCR-RFLP based assay on the first and/or second internal transcribed spacer (ITS1 and ITS2) of ribosomal DNA was developed for the detection of T. lacrymalis DNA in its putative vector(s). The sensitivity of the technique was also assessed. The restriction patterns obtained readily differentiated T. lacrymalis from four species of Musca (Diptera, Muscidae) (i.e. Musca autumnalis, Musca domestica, Musca larvipara and Musca osiris), which are potential vectors of equine eyeworms. The molecular assay presented herein is a useful tool to identify the intermediate host(s) of T. lacrymalis in natural conditions and to study its/their ecology and epidemiology. |
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Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy. dtraversa@unite.it |
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English |
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ISSN |
0890-8508 |
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Notes |
PMID:16038792 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2626 |
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Permanent link to this record |
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Author |
Stock, K.F.; Distl, O. |
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Title |
Evaluation of expected response to selection for orthopedic health and performance traits in Hanoverian Warmblood horses |
Type |
Journal Article |
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Year |
2005 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
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Volume |
66 |
Issue |
8 |
Pages |
1371-1379 |
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Keywords |
Animals; Bone Diseases/genetics/*veterinary; *Breeding; Genetic Predisposition to Disease; Horse Diseases/*genetics; Horses/*genetics; Joint Diseases/genetics/*veterinary; Selection (Genetics) |
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Abstract |
OBJECTIVE: To determine whether selection schemes accounting for orthopedic health traits were compatible with breeding progress in performance parameters in Hanoverian Warmblood horses. ANIMALS: 5,928 horses. PROCEDURE: Relative breeding values (RBVs) were predicted for osseous fragments in fetlock (metacarpo- and metatarsophalangeal) and tarsal joints, deforming arthropathy in tarsal joints, and pathologic changes in distal sesamoid bones. Selection schemes were developed on the basis of total indices for radiographic findings (TIR), dressage (TID), and jumping (TIJ). Response to selection was traced over 2 generations of horses for dressage and jumping ability and all-purpose breeding. Development of mean RBVs and mean total indices in sires and prevalences of orthopedic health traits in their offspring were used to assess response to selection. RESULTS: Giving equal weight toTIR andTID, TIJ, or a combined index of 60% TID and 40% TIJ, 43% to 53% of paternal grandsires and 70% to 82% of descending sires passed selection. In each case, RBVs and total indices increased by as much as 9% in selected sires, when compared with all sires, and prevalences of orthopedic health traits in offspring of selected sires decreased relatively by as much as 16%. When selection was exclusively based on TID, TIJ, or TID and TIJ, percentages of selected sires were 44% to 66% in the first and 73% to 84% in the second generation and TID and TIJ increased by 9% to 10% and 19% to 23%, respectively. CONCLUSIONS AND CLINICAL RELEVANCE: Compared with exclusively performance-based selection, percentages of selected sires changed slightly and breeding progress in TID, TIJ, or TID and TIJ was only slightly decreased; however, prevalences of orthopedic health traits decreased in offspring of TIR-selected sires. |
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Address |
Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), Bunteweg 17p, 30559 Hannover, Germany |
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English |
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ISSN |
0002-9645 |
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Notes |
PMID:16173480 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
3713 |
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Permanent link to this record |
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Author |
Chilton, N.B. |
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Title |
The use of nuclear ribosomal DNA markers for the identification of bursate nematodes (order Strongylida) and for the diagnosis of infections |
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Journal Article |
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Year |
2004 |
Publication |
Animal Health Research Reviews / Conference of Research Workers in Animal Diseases |
Abbreviated Journal |
Anim Health Res Rev |
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Volume |
5 |
Issue |
2 |
Pages |
173-187 |
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Keywords |
Animals; Birds; Cats; DNA Primers; DNA, Helminth/*analysis; DNA, Ribosomal/*analysis; Dogs; Horses; Molecular Diagnostic Techniques/veterinary; Ruminants; Strongylida/*genetics; Strongylida Infections/diagnosis/*veterinary |
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Abstract |
Many bursate nematodes are of major importance to animal health. Animals are often parasitized by multiple species that differ in their prevalence, relative abundance and/or pathogenicity. Implementation of effective management strategies for these parasites requires reliable methods for their detection in hosts, identification to the species level and measurement of intensity of infection. One major problem is the difficulty of accurately identifying and distinguishing many species of bursate nematode because of the remarkable morphological similarity of their eggs and larvae. The inability to identify, with confidence, individual nematodes (irrespective of their life-cycle stage) to the species level by morphological methods has often led to a search for species-specific genetic markers. Studies over the past 15 years have shown that sequences of the internal transcribed spacers of ribosomal DNA provide useful genetic markers, providing the basis for the development of PCR-based diagnostic tools. Such molecular methods represent powerful tools for studying the systematics, epidemiology and ecology of bursate nematodes and, importantly, for the specific diagnosis of infections in animals and humans, thus contributing to improved control and prevention strategies for these parasites. |
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Address |
Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada. neil.chilton@usask.ca |
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English |
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ISSN |
1466-2523 |
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Notes |
PMID:15984323 |
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no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2628 |
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Permanent link to this record |
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Author |
Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B. |
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Title |
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces |
Type |
Journal Article |
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Year |
2004 |
Publication |
Parasitology |
Abbreviated Journal |
Parasitology |
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Volume |
129 |
Issue |
Pt 6 |
Pages |
733-739 |
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Keywords |
Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics |
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Abstract |
Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae. |
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Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it |
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English |
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ISSN |
0031-1820 |
ISBN |
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Notes |
PMID:15648696 |
Approved |
no |
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Call Number |
Equine Behaviour @ team @ |
Serial |
2631 |
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Permanent link to this record |