Records |
Author |
Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B. |
Title |
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces |
Type |
Journal Article |
Year |
2004 |
Publication |
Parasitology |
Abbreviated Journal |
Parasitology |
Volume |
129 |
Issue |
Pt 6 |
Pages |
733-739 |
Keywords |
Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics |
Abstract |
Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae. |
Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it |
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Language |
English |
Summary Language |
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Original Title |
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Series Editor |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0031-1820 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:15648696 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2631 |
Permanent link to this record |
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Author |
Sloet van Oldruitenborgh-Oosterbaan, M.M.; Blok, M.B.; Begeman, L.; Kamphuis, M.C.D.; Lameris, M.C.; Spierenburg, A.J.; Lashley, M.J.J.O. |
Title |
Workload and stress in horses: comparison in horses ridden deep and round ('rollkur') with a draw rein and horses ridden in a natural frame with only light rein contact |
Type |
Journal Article |
Year |
2006 |
Publication |
Tijdschrift Voor Diergeneeskunde |
Abbreviated Journal |
Tijdschr Diergeneeskd |
Volume |
131 |
Issue |
5 |
Pages |
152-157 |
Keywords |
Animal Husbandry/methods; Animals; Biomechanics; Blood Glucose/analysis; Female; Heart Rate/physiology; Hematocrit/veterinary; Horses/blood/*physiology; Hydrocortisone/blood; Lactic Acid/blood; Physical Conditioning, Animal/adverse effects/*physiology; Stress, Physiological/blood/etiology/veterinary |
Abstract |
'Rollkur' or 'overbending' is the low and deep riding of a dressage horse during training or warming up. Lately, this technique has been criticized, and not necessarily objectively, on welfare grounds. To be able to evaluate these criticisms, more needs to be known about the workload and stress of horses being ridden 'rollkur'. The aim of the present study was to compare the workload of eight riding-school horses when being ridden deep and round with a draw rein ('rollkur') and when being ridden in a natural frame with only light rein contact ('free'). Workload (as measured by heart rate and blood lactate concentration) was slightly higher when horses were ridden 'rollkur' than when they were ridden 'free'. There were no differences in packed cell volume, or glucose and cortisol concentrations. No signs of uneasiness or stress could be determined when the horses were ridden 'rollkur'. Subjectively, all horses improved their way of moving during 'rollkur' and were more responsive to their rider. |
Address |
Department of Equine Sciences, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 12, 3584 CM Utrecht, The Netherlands. m.sloet@vet.uu.nl |
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English |
Summary Language |
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Original Title |
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Series Editor |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0040-7453 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:16532786 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
5638 |
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Author |
Boissevain, I. |
Title |
[Animal and human rights in installments] |
Type |
|
Year |
2007 |
Publication |
Tijdschrift Voor Diergeneeskunde |
Abbreviated Journal |
Tijdschr Diergeneeskd |
Volume |
132 |
Issue |
4 |
Pages |
132 |
Keywords |
Animals; Clinical Competence/*standards; Horse Diseases/*diagnosis; Horses; Humans; Netherlands; Time Factors; Veterinary Medicine/*methods/*standards |
Abstract |
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Address |
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Place of Publication |
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Editor |
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Language |
Dutch |
Summary Language |
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Original Title |
Dierenrechten en mensenrechten in termijnen |
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Series Title |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0040-7453 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:17366876 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4018 |
Permanent link to this record |
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Author |
Ganswindt, A.; Palme, R.; Heistermann, M.; Borragan, S.; Hodges, J.K. |
Title |
Non-invasive assessment of adrenocortical function in the male African elephant (Loxodonta africana) and its relation to musth |
Type |
Journal Article |
Year |
2003 |
Publication |
General and Comparative Endocrinology |
Abbreviated Journal |
Gen Comp Endocrinol |
Volume |
134 |
Issue |
2 |
Pages |
156-166 |
Keywords |
Adrenal Cortex/*metabolism/secretion; Adrenal Cortex Function Tests/methods/*veterinary; Adrenocorticotropic Hormone/physiology; Animals; Carbon Isotopes/diagnostic use; Chromatography, High Pressure Liquid/veterinary; Elephants/*metabolism/urine; Feces/*chemistry; Glucocorticoids/analysis/urine; Hydrocortisone/*analysis/diagnostic use/urine; Immunoenzyme Techniques/methods/veterinary; Male; Reproduction/physiology; Sexual Behavior, Animal/physiology; Stress, Psychological/diagnosis/*physiopathology; Testosterone/*analysis/diagnostic use/urine |
Abstract |
Adult male elephants periodically show the phenomenon of musth, a condition associated with increased aggressiveness, restlessness, significant weight reduction and markedly elevated androgen levels. It has been suggested that musth-related behaviours are costly and that therefore musth may represent a form of physiological stress. In order to provide data on this largely unanswered question, the first aim of this study was to evaluate different assays for non-invasive assessment of adrenocortical function in the male African elephant by (i) characterizing the metabolism and excretion of [3H]cortisol (3H-C) and [14C]testosterone (14C-T) and (ii) using this information to evaluate the specificity of four antibodies for determination of excreted cortisol metabolites, particularly with respect to possible cross-reactions with androgen metabolites, and to assess their biological validity using an ACTH challenge test. Based on the methodology established, the second objective was to provide data on fecal cortisol metabolite concentrations in bulls during the musth and non-musth condition. 3H-C (1 mCi) and 14C-T (100 microCi) were injected simultaneously into a 16 year old male and all urine and feces collected for 30 and 86 h, respectively. The majority (82%) of cortisol metabolites was excreted into the urine, whereas testosterone metabolites were mainly (57%) excreted into the feces. Almost all radioactive metabolites recovered from urine were conjugated (86% 3H-C and 97% 14C-T). In contrast, 86% and >99% of the 3H-C and 14C-T metabolites recovered from feces consisted of unconjugated forms. HPLC separations indicated the presence of various metabolites of cortisol in both urine and feces, with cortisol being abundant in hydrolysed urine, but virtually absent in feces. Although all antibodies measured substantial amounts of immunoreactivity after HPLC separation of peak radioactive samples and detected an increase in glucocorticoid output following the ACTH challenge, only two (in feces against 3alpha,11-oxo-cortisol metabolites, measured by an 11-oxo-etiocholanolone-EIA and in urine against cortisol, measured by a cortisol-EIA) did not show substantial cross-reactivity with excreted 14C-T metabolites and could provide an acceptable degree of specificity for reliable assessment of glucocorticoid output from urine and feces. Based on these findings, concentrations of immunoreactive 3alpha,11-oxo-cortisol metabolites were determined in weekly fecal samples collected from four adult bulls over periods of 11-20 months to examine whether musth is associated with increased adrenal activity. Results showed that in each male levels of these cortisol metabolites were not elevated during periods of musth, suggesting that in the African elephant musth is generally not associated with marked elevations in glucocorticoid output. Given the complex nature of musth and the variety of factors that are likely to influence its manifestation, it is clear, however, that further studies, particularly on free-ranging animals, are needed before a possible relationship between musth and adrenal function can be resolved. This study also clearly illustrates the potential problems associated with cross-reacting metabolites of gonadal steroids in EIAs measuring glucocorticoid metabolites. This has to be taken into account when selecting assays and interpreting results of glucocorticoid metabolite analysis, not only for studies in the elephant but also in other species. |
Address |
German Primate Centre, Department of Reproductive Biology, Kellnerweg 4, 37077 Gottingen, Germany. ganswindt@www.dpz.gdwg.de |
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English |
Summary Language |
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Series Editor |
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Abbreviated Series Title |
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Edition |
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ISSN |
0016-6480 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:14511986 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4085 |
Permanent link to this record |
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Author |
McGreevy, P.D.; French, N.P.; Nicol, C.J. |
Title |
The prevalence of abnormal behaviours in dressage, eventing and endurance horses in relation to stabling |
Type |
Journal Article |
Year |
1995 |
Publication |
The Veterinary record |
Abbreviated Journal |
Vet. Rec. |
Volume |
137 |
Issue |
2 |
Pages |
36-37 |
Keywords |
Animal Husbandry/*methods; Animals; *Behavior, Animal; Horse Diseases/*psychology; Horses; *Physical Conditioning, Animal; Prevalence; Questionnaires; *Stereotyped Behavior |
Abstract |
The behaviour of horses competing in different disciplines was studied and the relationship between the time they spent out of the stable and the prevalence of abnormal behaviour was examined. The owners of dressage, eventing and endurance horses were sent a questionnaire and a total of 1101 responses were received, giving data on 1750 horses. The behaviours studied were wood-chewing, weaving, crib-biting/wind-sucking and box-walking. The reported percentage prevalences of abnormal behaviour for the dressage, eventing and endurance horses were 32.5, 30.8 and 19.5, respectively. The relationship between the time spent in the stable and the prevalence of abnormal behaviour was examined by chi 2 tests which showed that there were significant linear trends for the eventing group (P < 0.001) and the dressage group (P < 0.05). It is concluded that the time a horse spends out of the stable is related to the discipline for which it is being trained and in dressage and eventing horses the time spent in a stable is correlated with an increased risk of abnormal behaviour. |
Address |
University of Bristol, Department of Clinical Veterinary Science, Langford |
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English |
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Series Editor |
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Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0042-4900 |
ISBN |
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Medium |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:8525580 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
89 |
Permanent link to this record |
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Author |
Ionita, J.C.; Poncet, P.A.; Doherr, M.G.; Steiger, A. |
Title |
[Evaluation of the quality of husbandry of Franches-Montagnes horses in their breeding farms] |
Type |
Journal Article |
Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
Volume |
148 |
Issue |
4 |
Pages |
191-197 |
Keywords |
Animal Husbandry/methods/*standards/statistics & numerical data; *Animal Welfare; Animals; Breeding/*methods/standards; Female; Floors and Floorcoverings; Horses/*physiology; Housing, Animal/*standards; Male; Poaceae; Questionnaires; Social Behavior; Switzerland |
Abstract |
The quality of husbandry of Franches-Montagnes horses (FM) in Switzerland is evaluated on the basis of an investigation carried out in 2002 by the Swiss FM breeding federation. Questionnaires were sent to 3500 of its members and the results include data from 968 breeding enterprises, housing a total of 3965 FM: 46.1% were breeding mares (61.0% with foal at foot), 26.5% young stock, 1.3% stallions and 26.0% non breeding stock (74.6% of which were pleasure horses and 25.4% working horses). 57.6% of the FM were housed in individual boxes with or without permanent outdoor access, 25.4% were hold in groups with or without permanent outdoor access, the remaining 17.0% were kept in standing stalls. 95.0% of the FM had at least visual contact with other equines and 99.2% had sufficient light in their stable. 88.1% were stabled on long stalk straw, while only 4.3% were bedded on other materials other than straw. The average time spent at pasture per horse and per week ranged from 96.5 +/- 51.6 hours in summer to 27.2 +/- 26.7 hours in winter. On average, a FM is used for 8.3 +/- 6.5 hours per week. Horses with an paddock at their disposal spend an average of 39.8 +/- 45.9 hours there per week. |
Address |
Chirurgische Tierklinik, Universitat Leipzig |
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Language |
French |
Summary Language |
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Original Title |
Les conditions de detention des chevaux de race franches-montagnes dans leurs exploitations d'elevage |
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Series Issue |
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Edition |
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ISSN |
0036-7281 |
ISBN |
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Area |
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Notes |
PMID:16703760 |
Approved |
no |
Call Number |
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Serial |
1872 |
Permanent link to this record |
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Author |
Poncet, P.A.; Ionita, J.C.; Doherr, M.G.; Steiger, A. |
Title |
[The influence of the socio-economic structure of the breeding farms of Franches-Montagnes horses on the conditions of husbandry] |
Type |
Journal Article |
Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
Volume |
148 |
Issue |
4 |
Pages |
183-189 |
Keywords |
Altitude; Animal Husbandry/methods/*standards; Animals; Breeding/*economics/standards; Demography; Female; Horses/*physiology; Housing, Animal/*standards; Male; Physical Conditioning, Animal/methods/*physiology; Questionnaires; Social Behavior; Socioeconomic Factors; Switzerland |
Abstract |
The socio-economic structure of the breeding farms of Franches-Montagnes horses (FM) in Switzerland is evaluated on the basis of an investigation carried out in 2002 by the Swiss FM breeding federation. Questionnaires were sent to 3500 of its members and the results include data from 968 breeding enterprises, housing a total of 3965 FM. The quality of the husbandry of FM varies according to factors such as the altitude and the geographical situation of the farms and studs. Socio-economic parameters, such as the role of FM in the business, their use (breeding, driving, riding) and the age and level of professional education of the owners may also have an effect on standards of husbandry. The results show that the owners for whom FM represent a source of income more frequently keep their horses in standing stalls, but give them more time to exercise at liberty than the horses belonging to amateur breeders. Younger and better educated breeders are more likely to house their animals in groups. |
Address |
Haras national suisse, Avenches |
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Language |
French |
Summary Language |
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Original Title |
L'influence de la structure socio-economique des exploitations d'elevage de chevaux de race franches-montagnes sur les conditions de detention |
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Edition |
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ISSN |
0036-7281 |
ISBN |
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Notes |
PMID:16703759 |
Approved |
no |
Call Number |
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Serial |
1873 |
Permanent link to this record |
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Author |
McBride, S.D.; Long, L. |
Title |
Management of horses showing stereotypic behaviour, owner perception and the implications for welfare |
Type |
Journal Article |
Year |
2001 |
Publication |
The Veterinary Record |
Abbreviated Journal |
Vet. Rec. |
Volume |
148 |
Issue |
26 |
Pages |
799-802 |
Keywords |
*Animal Husbandry/methods; *Animal Welfare; Animals; England/epidemiology; Horse Diseases/*epidemiology/*prevention & control/psychology; Horses; Humans; Interviews; *Perception; *Stereotyped Behavior |
Abstract |
A telephone survey was conducted of 100 racing stables, 100 riding schools and 100 competition establishments (8,427 horses in total) to determine what management practices were being applied to horses showing stereotypic behaviour, and to determine the underlying reasons for them by assessing the perceptions and opinions of the people working with the horses. The results indicated that horse owners are concerned about stereotypic behaviour, first, because it reduces the performance of the animal (31, 30 and 27 per cent of the owners of racing stables, riding schools and competition establishments respectively), secondly, because it has adverse clinical effects on the horse (52, 55 and 56 per cent), and thirdly, because it reduces the monetary value of the animal (45, 59 and 31 per cent). The belief that these behaviours are learnt or copied also affects the management of affected horses: they are not allowed on to the premises by 4, 32 and 17 per cent of owners of racing stables, riding schools and competition establishments, respectively; attempts are made to remove the causal factors of the stereotypy by 35, 43 and 36 per cent; the behaviours are physically prevented by 77, 67 and 79 per cent, and the affected horses are kept separate from other horses by 39, 30 and 48 per cent. |
Address |
Institute of Rural Studies, University of Wales, Aberystwyth |
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English |
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Edition |
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0042-4900 |
ISBN |
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Notes |
PMID:11467606 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
1918 |
Permanent link to this record |
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Author |
Musterle, B.; Furst, A.; Geyer, H.; Raber, M.; Weishaupt, M.A. |
Title |
[Interactive educational DVD on hoof protection, horseshoeing and diseases of the hoof] |
Type |
Journal Article |
Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
Volume |
148 |
Issue |
2 |
Pages |
81-85 |
Keywords |
Animals; *Education, Veterinary/methods; Foot Diseases/pathology/therapy/*veterinary; Hoof and Claw/anatomy & histology/*physiology; Horse Diseases/*pathology/therapy; Horses/anatomy & histology/*physiology; Humans; Shoes; Videodisc Recording |
Abstract |
Good cooperation between farrier, veterinarian and horse owner is an important prerequisite for optimal support of the horse with regards to shoeing and hoof health. The introduction of a joint educational aid aims to improve the level of education of both veterinarians and farriers. The interactive, multimedia approach represents an innovative new dimension in instruction techniques, predominantly provided through images and videos. The contents of the new teaching aid will focus on detailed anatomy of the foot and distal limb, as well as currently accepted shoeing practices and techniques and pathologic conditions of the hoof and foot. |
Address |
Pferdeklinik der Universitat Zurich |
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Language |
German |
Summary Language |
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Original Title |
Interaktives Lehrmittel Huf: Schutz, Beschlag und Erkrankungen |
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Edition |
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ISSN |
0036-7281 |
ISBN |
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Conference |
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Notes |
PMID:16509169 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4033 |
Permanent link to this record |
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Author |
Nicol, C.J.; Davidson, H.P.D.; Harris, P.A.; Waters, A.J.; Wilson, A.D. |
Title |
Study of crib-biting and gastric inflammation and ulceration in young horses |
Type |
Journal Article |
Year |
2002 |
Publication |
The Veterinary record |
Abbreviated Journal |
Vet. Rec. |
Volume |
151 |
Issue |
22 |
Pages |
658-662 |
Keywords |
Animal Husbandry/methods; Animals; Antacids/therapeutic use; *Behavior, Animal; Diet/veterinary; Endoscopy, Gastrointestinal/veterinary; Feces/chemistry; Female; Gastritis/diet therapy/physiopathology/*veterinary; Horse Diseases/diet therapy/*physiopathology/psychology; Horses; Hydrogen-Ion Concentration; Male; Random Allocation; Stereotyped Behavior/*physiology; Stomach Ulcer/diet therapy/physiopathology/*veterinary; Treatment Outcome; Weaning |
Abstract |
Nineteen young horses that had recently started to perform the stereotypy of crib-biting were compared with 16 non-stereotypic horses for 14 weeks. After initial observations of their behaviour and an endoscopic examination of the condition of their stomachs, the horses were randomly allocated to a control or an antacid diet At the start of the trial, the stomachs of the crib-biting foals were significantly more ulcerated and inflamed than the stomachs of the normal foals. In addition, the faecal pH of the crib-biting foals (6.05) was significantly lower than that of the normal foals (6.58). The antacid diet resulted in a significant improvement in the condition of the horses' stomachs. The crib-biting behaviour declined in most of the foals, regardless of their diet, but tended to decline to a greater extent in the foals on the antacid diet. |
Address |
Department of Clinical Veterinary Science, University of Bristol, Langford House, Bristol BS40 5DU |
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English |
Summary Language |
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Original Title |
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Series Editor |
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Abbreviated Series Title |
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Series Volume |
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Series Issue |
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Edition |
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ISSN |
0042-4900 |
ISBN |
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Area |
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Expedition |
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Conference |
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Notes |
PMID:12498408 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
83 |
Permanent link to this record |