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Author Maury, M.; Murphy, K.; Kumar, S.; Mauerer, A.; Lee, G. url  doi
openurl 
  Title Spray-drying of proteins: effects of sorbitol and trehalose on aggregation and FT-IR amide I spectrum of an immunoglobulin G Type Journal Article
  Year 2005 Publication European Journal of Pharmaceutics and Biopharmaceutics Abbreviated Journal Eur. J. Pharm. Biopharm.  
  Volume (up) 59 Issue 2 Pages 251-261  
  Keywords Immunoglobulin; Spray-drying; Stabilization; Sorbitol; Trehalose; Water replacement  
  Abstract An immunoglobulin G (IgG) was spray-dried on a Büchi 190 laboratory spray-dryer at inlet and outlet air temperatures of 130 and 190°C, respectively. The IgG solution contains initially 115mg/ml IgG plus 50mg/ml sorbitol. After dialysis, at least 80% of low molecular weight component was removed. After spray-drying the dialyzed IgG and immediate redissolution of the powder, an increase in aggregates from 1 to 17% occurred. A major shift towards increase β-sheet structure was detected in the spray-dried solid, which, however, reverted to native structure on redissolution of the powder. A correlation between aggregation determined by size exclusion chromatography and alterations in secondary structure determined by Fourier transformation infra-red spectroscopy could not therefore be established. On spray-drying a non-dialyzed, sorbitol-containing IgG only some 0.7% aggregates were formed. The sorbitol is therefore evidently able to stabilize partially the IgG during the process of spray-drying. Addition of trehalose to the liquid feed produced quantitatively the same stabilizing action on the IgG during spray-drying as did the sorbitol. This finding again points towards a water replacement stabilization mechanism. The IgG spray-dried powder prepared from the dialyzed liquid feed showed continued substantial aggregation on dry storage at 25°C. This was substantially less in the non-dialyzed, sorbitol-containing spray-dried powder. Addition of trehalose to both dialyzed and non-dialyzed system produced substantial improvement in storage stability and reduction in aggregate formation in storage. The quantitative stabilizing effect of the trehalose was only slightly higher than that of the sorbitol. Taken together, these results indicate that both the sorbitol and trehalose stabilize the IgG primarily by a water replacement mechanism rather than by glassy immobilization. The relevance of this work is its questioning of the importance of the usually considered dominance of glassy stabilization of protein in dried systems of high glass transition temperature, such as trehalose. The low glass transition temperature sorbitol produces almost equal process and storage stability in this case.  
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  ISSN 0939-6411 ISBN Medium  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6515  
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Author Gholib, G.; Heistermann, M.; Agil, M.; Supriatna, I.; Purwantara, B.; Nugraha, T.P.; Engelhardt, A. url  doi
openurl 
  Title Comparison of fecal preservation and extraction methods for steroid hormone metabolite analysis in wild crested macaques Type Journal Article
  Year 2018 Publication Primates Abbreviated Journal Primates  
  Volume (up) 59 Issue 3 Pages 281-292  
  Keywords  
  Abstract Since the non-invasive field endocrinology techniques were developed, several fecal preservation and extraction methods have been established for a variety of species. However, direct adaptation of methods from previous studies for use in crested macaques should be taken with caution. We conducted an experiment to assess the accuracy and stability of fecal estrogen metabolite (E1C) and glucocorticoid metabolite (GCM) concentrations in response to several preservation parameters: (1) time lag between sample collection and fecal preservation; (2) long-term storage of fecal samples in 80% methanol (MeOH) at ambient temperature; (3) different degrees of feces drying temperature using a conventional oven; and (4) different fecal preservation techniques (i.e., freeze-drying, oven-drying, and field-friendly extraction method) and extraction solvents (methanol, ethanol, and commercial alcohol). The study used fecal samples collected from crested macaques (Macaca nigra) living in the Tangkoko Reserve, North Sulawesi, Indonesia. Samples were assayed using validated E1C and GCM enzyme immunoassays. Concentrations of E1C and GCM in unprocessed feces stored at ambient temperature remained stable for up to 8 h of storage after which concentrations of both E1C and GCM changed significantly compared to controls extracted at time 0. Long-term storage in 80% MeOH at ambient temperature affected hormone concentrations significantly with concentrations of both E1C and GCM increasing after 6 and 4 months of storage, respectively. Drying fecal samples using a conventional oven at 50, 70, and 90 °C did not affect the E1C concentrations, but led to a significant decline for GCM concentrations in samples dried at 90 °C. Different fecal preservation techniques and extraction solvents provided similar results for both E1C and GCM concentrations. Our results confirm previous studies that prior to application of fecal hormone analysis in a new species, several preservation parameters should be evaluated for their effects on hormone metabolite stability. The results also provide several options for fecal preservation, extraction, and storage methods that can be selected depending on the condition of the field site and laboratory.  
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  ISSN 1610-7365 ISBN Medium  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Gholib2018 Serial 6521  
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Author Iliopoulos, Y.; Youlatos, D.; Sgardelis, S. url  doi
openurl 
  Title Wolf pack rendezvous site selection in Greece is mainly affected by anthropogenic landscape features Type Journal Article
  Year 2013 Publication Eur J Wildl Res Abbreviated Journal  
  Volume (up) 60 Issue Pages  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Iliopoulos2013 Serial 6478  
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Author Pérez-Barbería, F.J.; Shultz, S.; Dunbar, R.I. url  doi
openurl 
  Title Evidence for coevolution of sociality and relative brain size in three orders of mammals Type Journal Article
  Year 2007 Publication Evolution Abbreviated Journal  
  Volume (up) 61 Issue Pages  
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  Call Number Equine Behaviour @ team @ Pérez-Barbería2007 Serial 6221  
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Author Pongrácz, P.; Miklósi, Á.; Kubinyi, E.; Gurobi, K.; Topál, J.; Csányi, V. url  doi
openurl 
  Title Social learning in dogs: the effect of a human demonstrator on the performance of dogs in a detour task Type Journal Article
  Year 2001 Publication Animal Behaviour. Abbreviated Journal Anim. Behav.  
  Volume (up) 62 Issue 6 Pages 1109-1117  
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  Abstract We recorded the behaviour of dogs in detour tests, in which an object (a favourite toy) or food was placed behind a V-shaped fence. Dogs were able to master this task; however, they did it more easily when they started from within the fence with the object placed outside it. Repeated detours starting from within the fence did not help the dogs to obtain the object more quickly if in a subsequent trial they started outside the fence with the object placed inside it. While six trials were not enough for the dogs to show significant improvement on their own in detouring the fence from outside, demonstration of this action by humans significantly improved the dogs' performance within two-three trials. Owners and strangers were equally effective as demonstrators. Our experiments show that dogs are able to rely on information provided by human action when confronted with a new task. While they did not copy the exact path of the human demonstrator, they easily adopted the detour behaviour shown by humans to reach their goal.  
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  Notes Approved no  
  Call Number refbase @ user @ Serial 847  
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Author Lefebvre, L.; Reader, S.M.; Sol, D. doi  openurl
  Title Brains, Innovations and Evolution in Birds and Primates Type Journal Article
  Year 2004 Publication Brain, Behavior and Evolution Abbreviated Journal Brain. Behav. Evol.  
  Volume (up) 63 Issue 4 Pages 233-246  
  Keywords Innovation W Brain evolution W Hyperstriatum ventrale W Neostriatum W Isocortex W Birds W Primates W Tool use W Invasion biology  
  Abstract Abstract

Several comparative research programs have focusedon the cognitive, life history and ecological traits thataccount for variation in brain size. We review one ofthese programs, a program that uses the reported frequencyof behavioral innovation as an operational measureof cognition. In both birds and primates, innovationrate is positively correlated with the relative size of associationareas in the brain, the hyperstriatum ventrale andneostriatum in birds and the isocortex and striatum inprimates. Innovation rate is also positively correlatedwith the taxonomic distribution of tool use, as well asinterspecific differences in learning. Some features ofcognition have thus evolved in a remarkably similar wayin primates and at least six phyletically-independent avianlineages. In birds, innovation rate is associated withthe ability of species to deal with seasonal changes in theenvironment and to establish themselves in new regions,and it also appears to be related to the rate atwhich lineages diversify. Innovation rate provides a usefultool to quantify inter-taxon differences in cognitionand to test classic hypotheses regarding the evolution ofthe brain.
 
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  ISSN 0006-8977 ISBN Medium  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 4738  
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Author Mori, E.; Benatti, L.; Lovari, S.; Ferretti, F. url  doi
openurl 
  Title What does the wild boar mean to the wolf? Type Journal Article
  Year 2016 Publication European Journal of Wildlife Research Abbreviated Journal  
  Volume (up) 63 Issue 1 Pages 9  
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  Abstract Generalist predators are expected to shape their diets according to the local availability of prey species. In turn, the extent of consumption of a prey would be influenced by the number of alternative prey species. We have tested this prediction by considering the wild boar and the grey wolf: two widespread species whose distribution ranges overlap largely in Southern Europe, e.g. in Italy. We have reviewed 16 studies from a total of 21 study areas, to assess whether the absolute frequency of occurrence of wild boar in the wolf diet was influenced by (i) occurrence of the other ungulate species in diet and (ii) the number of available ungulate species. Wild boar turned out to be the main prey of the wolf (49% occurrence, on average), followed by roe deer (24%) and livestock (18%). Occurrence of wild boar in the wolf diet decreased with increasing usage of roe deer, livestock, and to a lower extent, chamois and red deer. The number of prey species did not influence the occurrence of wild boar in the wolf diet. The wild boar is a gregarious, noisy and often locally abundant ungulate, thus easily detectable, to a predator. In turn, the extent of predation on this ungulate may not be influenced so much by the availability of other potential prey. Heavy artificial reductions of wild boar numbers, e.g. through numerical control, may concentrate predation by wolves on alternative prey (e.g. roe deer) and/or livestock, thus increasing conflicts with human activities.  
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  ISSN 1439-0574 ISBN Medium  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Mori2016 Serial 6689  
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Author Kruska, D.C.T. url  doi
openurl 
  Title On the evolutionary significance of encephalization in some eutherian mammals: effects of adaptive radiation, domestication, and feralization Type Journal Article
  Year 2005 Publication Brain Behav Evol Abbreviated Journal  
  Volume (up) 65 Issue Pages  
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  Call Number Equine Behaviour @ team @ Kruska2005 Serial 6235  
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Author Zlatanova, D.; Ahmed, A.; Valasseva, A.; Genov, P. openurl 
  Title Adaptive Diet Strategy of the Wolf (Canis lupus L.) in Europe: a Review Type Journal Article
  Year 2014 Publication ACTA ZOOLOGICA BULGARICA Abbreviated Journal Acta zool. bulg.  
  Volume (up) 66 Issue 4 Pages 439-452  
  Keywords Wolf, Canis lupus, prey, adaptive strategy  
  Abstract The diet strategy of the wolf in Europe is reviewed on the basis of 74 basic and 14 additional literature

sources. The comparative analysis reveals clear dependence on the latitude (and, therefore, on the changing

environmental conditions) correlated with the wild ungulate abundance and diversity. Following a

geographic pattern, the wolf is specialised on different species of ungulates: moose and reindeer in Scandinavia,

red deer in Central and Eastern Europe and wild boar in Southern Europe. Where this large prey

is taken, the roe deer is hunted with almost the same frequency in every region. The wolf diet in Europe

shows two ecological adaptations formed by a complex of variables: 1. Wolves living in natural habitats

with abundance of wild ungulates feed mainly on wild prey. 2. In highly anthropogenic habitats, with low

abundance of wild prey, wolves feed on livestock (where husbandry of domestic animals is available) and

take also a lot of plant food, smaller prey (hares and rodents) and garbage food. The frequency of occurrence

of wild ungulates in the diet of wolves in North Europe varies from 54.0% in Belarus to 132.7% in

Poland, while that of livestock is in the range from 0.4% in Norway to 74.9% in Belarus. In South Europe,

the frequency of occurrence of wild prey varies from 0% in Italy and Spain to 136.0% in Italy, while of domestic

ungulates ranges between 0% and 100% in Spain. The low density or lack of wild prey triggers the

switch of the wolf diet to livestock, plant food (32.2-85% in Italy) or even garbage (up to 41.5% in Italy).
 
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6388  
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Author Harris, F. url  doi
openurl 
  Title On the Use of Windows for Harmonic Analysis with the Discrete Fourier Transform Type Journal Article
  Year 1978 Publication Proc IEEE Abbreviated Journal  
  Volume (up) 66 Issue Pages  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Harris1978 Serial 6486  
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