Abstract: Behavioural tests using operant conditioning were conducted to examine how miniature pigs discriminate between people. During a 3-week handling period, six 8-week-old pigs were touched and fed raisins as a reward whenever they approached their handler. In subsequent training, the handler and a non-handler wearing dark blue and white coveralls, respectively, and wearing different eau de toilette fragrances sat at each end of a Y-maze. Pigs were rewarded with raisins when they chose the handler. Successful discrimination occurred when the pig chose the handler at least 15 times in 20 trials (P<0.05: by χ2 test). When all pigs exhibited successful discrimination under these standard conditions, they were exposed to Experiments 1 through 4. In Experiment 1, (1) handler and non-handler exchanged colours of coveralls; (2) handler and non-handler exchanged eau de toilette; (3) handler and non-handler exchanged both cues. The non-handler was chosen significantly more often following the exchange of coverall colours and the exchange of both coverall colours and eau de toilette. However, the handler was chosen significantly more frequently following exchange of eau de toilette only. In Experiment 2, when both handler and non-handler wore coveralls of the handler's original colour, the pigs had difficulty discriminating between them. In Experiment 3, both handler and non-handler wore coveralls of new colours. The pigs easily chose the handler wearing red or blue vs. white coveralls. In Experiment 4, (1) two novel people wore coveralls of the original colours of handler and non-handler; (2) the test with the original experimenters was conducted under the original conditions but in a novel place. Between novel people, the one wearing the handler's original colour of coveralls was preferentially chosen by the pigs. The pigs had difficulty discriminating the handler from the non-handler in a novel place. Pigs appear to discriminate between a familiar handler and a non-familiar person based primarily on visual cues, prominent of which is colour of clothing.

Abstract: The oxidation-reduction reaction of horse heart cytochrome c and cytochrome c (552, Thermus thermophilus), which is highly thermoresistant, was studied by temperature-jump method. Ferrohexacyanide was used as reductant. Thermodynamic and activation parameters of the reaction obtained for both cytochromes were compared with each other. The results of this showed that (1) the redox potential of cytochrome c-552,+0.19 V, is markedly less than that of horse heart cytochrome c. (2) [up triangle, open]Hox++ of cytochrome c-552 is considerably lower than that of horse heart cytochrome c. (3) [up triangle, open]Hox++ and [up triangle, open]Sred++ of cytoochrome c-552 are more negative than those of horse heart cytochrome c. (4) kred of cytochrome c-552 is much lower than that of horse heart cytochrome c at room temperature.

Abstract: Poaching is a widespread and well-appreciated problem for the conservation of many threatened species. Because poaching is illegal, there is strong incentive for poachers to conceal their activities, and consequently, little data on the effects of poaching on population dynamics are available. Quantifying poaching mortality should be a required knowledge when developing conservation plans for endangered species but is hampered by methodological challenges. We show that rigorous estimates of the effects of poaching relative to other sources of mortality can be obtained with a hierarchical state-space model combined with multiple sources of data. Using the Scandinavian wolf (Canis lupus) population as an illustrative example, we show that poaching accounted for approximately half of total mortality and more than two-thirds of total poaching remained undetected by conventional methods, a source of mortality we term as 'cryptic poaching'. Our simulations suggest that without poaching during the past decade, the population would have been almost four times as large in 2009. Such a severe impact of poaching on population recovery may be widespread among large carnivores. We believe that conservation strategies for large carnivores considering only observed data may not be adequate and should be revised by including and quantifying cryptic poaching.

Abstract: Book Description
Although animals are widely employed as research subjects, it is only recently that we have acknowledged the bond that frequently, perhaps inevitably, develops between subject and researcher. Whatever the qualities of this relationship, an increasing body of evidence suggests that it may result in profound behavioural and physiological changes in the animal subject. Such effects are apparent in behavioural studies conducted in both laboratory and field settings. They also appear in physiological studies ranging from the biomedical (e.g. heart rate, blood pressure, immunological changes) to animal science (e.g. growth, reproduction). Such effects are not confined to obvious cases involving primates and dogs, but appear in unexpected animals like chickens, reptiles and even octopuses. Despite the fact that most researchers are trained to minimise or avoid such interactions, they continue to occur. This book, the first of its kind to address this issue systematically, describes many examples of this “inevitable bond” between scientist and animal. This discussion will allow researchers to anticipate these potentially confounding effects and take advantage of such relationships in designing more effective and humane environments for animal subjects.

Abstract: Introduction
Many scientists agree that information on the learning abilities of horses should be used in developing training methods (Fiske and Potter, 1979; Mader and Price, 1980; McCall, 1990) but nevertheless research where such knowledge is put to the test is limited (Flannery, 1997). Foals that are handled continuously between 4 and 22 months of age were quicker learners and were easier to train than those that got less handling (Fiske and Potter, 1979). The handling involved being lead, brushed, and acclimated to restraint. In some studies more relaxed foals learned more and were easier to train later on than stressed foals (Fiske and Potter, 1979; Heird et al, 1986) while no such correlation was found in another study (Mader and Price 1980). It is commonly believed, and has been established with tests, that younger horses are quicker-learners than mature (Mader and Price, 1980; Houpt, 1982), but the question remains to be answered if a certain age is better than another and if so, if the optimum time differs between breeds.
The idea of imprint training (Miller, 2000) is controversial but seems to be popular in the USA. Miller found that foals that had been treated by veterinarians at birth were easier to handle later on. He associated this with what Konrad Lorenz called imprint learning (1937), which involved irreversible learning taking place during a sensitive period early in life. Miller claims that the idea has been tested scientifically but no such papers are covered by Wed of Science. To our knowledge one systematic research has been carried out whose results will be published this year (Jennifer Williams, personal communication). Miller (2000) claims that it is very important to stroke and handle the foals quickly after birth (hence the reference to the imprinting concept) and desensitize them to touch and other interventions. A study by Mal et al (1994) did not support the claim that very young foals are more sensitive to treatment than older foals. It is possible that what we are witnessing when the foals seem relaxed after stroking is not “imprinting” but “learned helplessness”.
In Iceland where mares give birth out in the field within the herd (usually herds of mares, sub-adults and sometimes geldings) it is customary not to disturb the mare and foal for the first hours of the foal's life. That fact and the custom in many places to let the foals stay with their dams within large groups in the wilderness from 1-2 months of age till autumn, would mean that continuous handling would not have a practical value for most Icelandic horse owners. We wanted to test the idea that early handling and training of foals should calm the foals considerably and teach them valuable lessons, which would make training later on easier.