|
Records |
Links |
|
Author |
Reimers, M.; Schwarzenberger, F.; Preuschoft, S. |
|
|
Title |
Rehabilitation of research chimpanzees: stress and coping after long-term isolation |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm Behav |
|
|
Volume |
51 |
Issue |
3 |
Pages |
428-435 |
|
|
Keywords |
Adaptation, Psychological/*physiology; Animals; *Animals, Laboratory; Exploratory Behavior; Hydrocortisone/analysis/metabolism; Male; Models, Biological; *Pan troglodytes; Social Dominance; Social Environment; *Social Isolation/psychology; Stress/*rehabilitation/veterinary; Time |
|
|
Abstract |
We report on the permanent retirement of chimpanzees from biomedical research and on resocialization after long-term social isolation. Our aim was to investigate to what extent behavioral and endocrine measures of stress in deprived laboratory chimpanzees can be improved by a more species-typical social life style. Personality in terms of novelty responses, social dominance after resocialization and hormonal stress susceptibility were affected by the onset of maternal separation of infant chimpanzees and duration of deprivation. Chimpanzees, who were separated from their mothers at a younger age and kept in isolation for more years appeared to be more timid personalities, less socially active, less dominant and more susceptible to stress, as compared to chimpanzees with a less severe deprivation history. However, permanent retirement from biomedical research in combination with therapeutic resocialization maximizing chimpanzees' situation control resulted in reduced fecal cortisol metabolite levels. Our results indicate that chimpanzees can recover from severe social deprivation, and may experience resocialization as less stressful than solitary housing. |
|
|
Address |
Department of Natural Sciences, Institute of Biochemistry, University of Veterinary Medicine, Veterinarplatz 1, 1210 Vienna, Austria. reimers@wild-vet.at |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0018-506X |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17292368 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
4188 |
|
Permanent link to this record |
|
|
|
|
Author |
Kralj-Fiser, S.; Scheiber, I.B.R.; Blejec, A.; Moestl, E.; Kotrschal, K. |
|
|
Title |
Individualities in a flock of free-roaming greylag geese: behavioral and physiological consistency over time and across situations |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm Behav |
|
|
Volume |
51 |
Issue |
2 |
Pages |
239-248 |
|
|
Keywords |
Animals; Behavior, Animal/*physiology; Corticosterone/metabolism; Feces; Female; Geese/*physiology; Individuality; Male; Personality/*physiology; Population Density; Reproducibility of Results; Seasons; *Social Behavior; Social Environment; Testosterone/*metabolism |
|
|
Abstract |
The concept of personality implies individual differences in behavior and physiology that show some degree of repeatability/consistency over time and across contexts. Most studies of animal personality, particularly studies of individuals' variation in physiological mechanisms, have been conducted on selected individuals in controlled conditions. We attempted to detect consistent behaviors as well as physiological patterns in greylag ganders (Anser anser) from a free-roaming flock living in semi-natural conditions. We tested 10 individuals repeatedly, in a handling trial, resembling tests for characterization of “temperaments” in captive animals. We recorded the behavior of the same 10 individuals during four situations in the socially intact flock: (1) a “low density feeding condition”, (2) a “high density feeding condition”, (3) a “low density post-feeding situation” and (4) while the geese rested. We collected fecal samples for determination of excreted immuno-reactive corticosterone (BM) and testosterone metabolites (TM) after handling trials, as well as the “low density feeding” and the “high density feeding” conditions. BM levels were very highly consistent over the repeats of handling trials, and the “low density feeding condition” and tended to be consistent over the first two repeats of the “high density feeding condition”. Also, BM responses tended to be consistent across contexts. Despite seasonal variation, there tended to be inter-test consistency of TM, which pointed to some individual differences in TM as well. Aggressiveness turned out to be a highly repeatable trait, which was consistent across social situations, and tended to correlate with an individual's resistance during handling trials. Also, “proximity to the female partner” and “sociability” – the average number of neighboring geese in a close distance while resting – were consistent. We conclude that aggressiveness, “affiliative tendencies” and levels of excreted corticosterone and testosterone metabolites may be crucial factors of personality in geese. |
|
|
Address |
University of Ljubljana, Biotechnical faculty, Slovenia. simona.kralj@guest.arnes.si |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0018-506X |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17196200 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
4189 |
|
Permanent link to this record |
|
|
|
|
Author |
Franceschini, C.; Siutz, C.; Palme, R.; Millesi, E. |
|
|
Title |
Seasonal changes in cortisol and progesterone secretion in Common hamsters |
Type |
Journal Article |
|
Year |
2007 |
Publication |
General and Comparative Endocrinology |
Abbreviated Journal |
Gen Comp Endocrinol |
|
|
Volume |
152 |
Issue |
1 |
Pages |
14-21 |
|
|
Keywords |
Animals; Cortisone/*secretion; Cricetinae/*metabolism; Feces/chemistry; Female; Lactation/physiology; Male; Pregnancy; Progesterone/*secretion; Reproduction/physiology; *Seasons; Sexual Behavior, Animal/physiology |
|
|
Abstract |
In this study, we investigated endocrine factors and behaviour in free-living Common hamsters (Cricetus cricetus) during reproductive and non-reproductive periods of the annual cycle. We applied a non-invasive method to gain information on seasonal changes in adrenocortical activity in male and female hamsters by analysing faecal glucocorticoid metabolite concentrations (FCM). In addition, plasma progesterone concentrations were monitored in females throughout the non-hibernation season. The animals were live-trapped from spring emergence until the onset of hibernation in autumn. Reproductive status was determined at capture and blood and faecal samples were collected. During behavioural observations, agonistic and sexual interactions were recorded. FCM concentrations were significantly higher in males than in females during the reproductive period. In males, a pronounced increase in FCM during the reproductive period coincided with high frequencies of intrasexual aggression. In females, FCM levels remained relatively constant. Aggressive behaviour in females increased during the reproductive period, but was much less frequent than in males. Females, which successfully raised a second litter after a postpartum oestrus and concurrent lactation and gestation had lower FCM levels than individuals, which lost their second litter after parturition. As expected, plasma progesterone concentrations were low before and after the reproductive period. During gestation, levels peaked and remained elevated during lactation. The results of this field study provide insight in critical periods associated with reproduction in male and female Common hamsters. |
|
|
Address |
Department of Behavioural Biology, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. claudia.franceschini@univie.ac.ct |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0016-6480 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17408667 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
4076 |
|
Permanent link to this record |
|
|
|
|
Author |
Muscatello, G.; Gilkerson, J.R.; Browning, G.F. |
|
|
Title |
Comparison of two selective media for the recovery, isolation, enumeration and differentiation of Rhodococcus equi |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
|
|
Volume |
119 |
Issue |
2-4 |
Pages |
324-329 |
|
|
Keywords |
|
|
|
Abstract |
The use of selective media to facilitate the isolation of Rhodococcus equi from environmental and clinical samples has aided studies of the ecology of R. equi and the epidemiology of disease caused by R. equi. Here, we compared the efficacy of two selective media (NANAT and modified CAZ-NB) for the recovery of six defined strains of R. equi and for the isolation and enumeration of both avirulent and virulent R. equi from 60 paired soil samples from horse farms using colony blotting and DNA hybridisation. No difference was found between the two media in the recoverability of defined strains of R. equi or the proportion of soil cultures positive for R. equi or virulent R. equi. NANAT medium was significantly less inhibitory of bacterial growth from soil culture compared to mCAZ-NB (P = 0.001), but there was no difference between the media in the number of R. equi colonies recovered. Soil cultured on mCAZ-NB medium yielded a significantly greater number of virulent R. equi colonies than NANAT (P = 0.03). The proportion of R. equi that were virulent in soil cultures on mCAZ-NB (32%) was more than three times that seen in cultures on NANAT (9%). Thus modified CAZ-NB appeared to be a better selective media for studies where the optimal recovery of virulent R. equi is required, such as in studies of the gastrointestinal carriage of virulent R. equi and of subclinically infected foals. |
|
|
Address |
Equine Infectious Disease Laboratory, School of Veterinary Science, The University of Melbourne, Victoria 3010, Australia. mug@unimelb.edu.au |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0378-1135 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17084043 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2621 |
|
Permanent link to this record |
|
|
|
|
Author |
Singer, E.R.; Barnes, J.; Saxby, F.; Murray, J.K. |
|
|
Title |
Injuries in the event horse: Training versus competition |
Type |
Journal Article |
|
Year |
2007 |
Publication |
|
Abbreviated Journal |
Vet J |
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
Two related studies on injuries sustained by event horses during competition and during training are reported. During the cross-country phase of competition, the most common injuries were lacerations and abrasions to the carpus and stifle. Superficial digital flexor tendonitis and exertional rhabdomyolysis were significantly more common during Cours Complete Internationale (CCI) competitions compared to one-day event (ODE) competitions. The difference in injury types at ODEs and CCI competitions probably relates to the increased athletic demands of the CCI and the closer veterinary observation at these competitions. The results of the training study indicate that 21% of horses intending to compete in a CCI did not start due to injury. Forty-three percent of these injuries involved soft tissue structures with injuries to the superficial digital flexor tendon and the suspensory ligament each accounting for 33%. The most important area for future research is investigation of the risk factors for these career-threatening soft tissue injuries. |
|
|
Address |
Division of Equine Studies, University of Liverpool, Leahurst, Neston, South Wirral, CH64 7TE, UK |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
1090-0233 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17204438 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
4025 |
|
Permanent link to this record |
|
|
|
|
Author |
Bannasch, D.; Rinaldo, C.; Millon, L.; Latson, K.; Spangler, T.; Hubberty, S.; Galuppo, L.; Lowenstine, L. |
|
|
Title |
SRY negative 64,XX intersex phenotype in an American saddlebred horse |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Veterinary Journal (London, England : 1997) |
Abbreviated Journal |
Vet J |
|
|
Volume |
173 |
Issue |
2 |
Pages |
437-439 |
|
|
Keywords |
Animals; Female; Genitalia/abnormalities; Hermaphroditism/*veterinary; Horse Diseases/*diagnosis/genetics; Horses/*genetics/*physiology; Karyotyping; Phenotype; Sex Differentiation; Sex Differentiation Disorders/diagnosis/veterinary; Sex-Determining Region Y Protein/genetics/*metabolism |
|
|
Abstract |
A female American saddlebred horse was presented for surgical correction of a possible pseudohermaphrodite condition. The horse had abnormal external genitalia and exhibited stallion-like behaviour. No evidence of uterine or ovarian tissue was identified on laparoscopic examination, but hypoplastic testicular-like tissue was removed, although this was found to contain no spermatogonia upon histopathological examination. A karyotype was performed and showed the normal chromosomal complement for a female horse (64,XX). Polymerase chain reaction to detect the SRY gene was negative in peripheral blood as well as the testicular-like tissue. This case represents the first report of an SRY negative XX-male sex reversal intersex phenotype, which is a potentially inherited condition, in an American saddlebred horse. |
|
|
Address |
Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA 95616, USA. dlbannasch@ucdavis.edu |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
1090-0233 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:16386440 |
Approved |
no |
|
|
Call Number |
|
Serial |
1882 |
|
Permanent link to this record |
|
|
|
|
Author |
Overli, O.; Sorensen, C.; Pulman, K.G.T.; Pottinger, T.G.; Korzan, W.; Summers, C.H.; Nilsson, G.E. |
|
|
Title |
Evolutionary background for stress-coping styles: relationships between physiological, behavioral, and cognitive traits in non-mammalian vertebrates |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Neuroscience and Biobehavioral Reviews |
Abbreviated Journal |
Neurosci Biobehav Rev |
|
|
Volume |
31 |
Issue |
3 |
Pages |
396-412 |
|
|
Keywords |
Adaptation, Psychological/*physiology; Animals; Behavior, Animal/*physiology; Biogenic Monoamines/physiology; Brain/physiology; Cognition/*physiology; Evolution; Glucocorticoids/*physiology; Individuality; Lizards; Oncorhynchus mykiss; Social Dominance; Stress, Psychological/*psychology |
|
|
Abstract |
Reactions to stress vary between individuals, and physiological and behavioral responses tend to be associated in distinct suites of correlated traits, often termed stress-coping styles. In mammals, individuals exhibiting divergent stress-coping styles also appear to exhibit intrinsic differences in cognitive processing. A connection between physiology, behavior, and cognition was also recently demonstrated in strains of rainbow trout (Oncorhynchus mykiss) selected for consistently high or low cortisol responses to stress. The low-responsive (LR) strain display longer retention of a conditioned response, and tend to show proactive behaviors such as enhanced aggression, social dominance, and rapid resumption of feed intake after stress. Differences in brain monoamine neurochemistry have also been reported in these lines. In comparative studies, experiments with the lizard Anolis carolinensis reveal connections between monoaminergic activity in limbic structures, proactive behavior in novel environments, and the establishment of social status via agonistic behavior. Together these observations suggest that within-species diversity of physiological, behavioral and cognitive correlates of stress responsiveness is maintained by natural selection throughout the vertebrate sub-phylum. |
|
|
Address |
Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, P.O. Box 5003, N-1432 As, Norway. oyvind.overli@umb.no |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0149-7634 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17182101 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2801 |
|
Permanent link to this record |
|
|
|
|
Author |
Pickering, C.M.; Hill, W. |
|
|
Title |
Impacts of recreation and tourism on plant biodiversity and vegetation in protected areas in Australia |
Type |
Journal Article |
|
Year |
2007 |
Publication |
|
Abbreviated Journal |
J Environ Manage |
|
|
Volume |
|
Issue |
|
Pages |
|
|
|
Keywords |
|
|
|
Abstract |
This paper reviews recent research into the impact of recreation and tourism in protected areas on plant biodiversity and vegetation communities in Australia. Despite the international significance of the Australian flora and increasing visitation to protected areas there has been limited research on recreational and tourism impacts in Australia. As overseas, there are obvious direct impacts of recreation and tourism such as clearing of vegetation for infrastructure or damage from trampling, horse riding, mountain biking and off road vehicles. As well, there are less obvious but potentially more severe indirect impacts. This includes self-propagating impacts associated with the spread of some weeds from trails and roads. It also includes the severe impact on native vegetation, including many rare and threatened plants, from spread of the root rot fungus Phytopthora cinnamomi. This review highlights the need for more recreational ecology research in Australia. |
|
|
Address |
International Centre for Ecotourism Research, Griffith University, PMB 50 Gold Coast Mail Centre, Gold Coast, Qld. 9726, Australia |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0301-4797 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17234325 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2620 |
|
Permanent link to this record |
|
|
|
|
Author |
Petter-Puchner, A.H.; Froetscher, W.; Krametter-Froetscher, R.; Lorinson, D.; Redl, H.; van Griensven, M. |
|
|
Title |
The long-term neurocompatibility of human fibrin sealant and equine collagen as biomatrices in experimental spinal cord injury |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft fur Toxikologische Pathologie |
Abbreviated Journal |
Exp Toxicol Pathol |
|
|
Volume |
58 |
Issue |
4 |
Pages |
237-245 |
|
|
Keywords |
Animals; Axotomy; Biocompatible Materials/*therapeutic use; Collagen/*therapeutic use; Fibrin Tissue Adhesive/*therapeutic use; Horses; Humans; Immunohistochemistry; Male; Motor Activity/physiology; Nerve Regeneration/*physiology; Rats; Recovery of Function; Spinal Cord/pathology/physiology; Spinal Cord Injuries/pathology/*therapy; Thoracic Vertebrae |
|
|
Abstract |
INTRODUCTION: While fibrin sealant (FS) and equine collagen (EC) have been used as scaffold materials in experimental spinal cord injury (SCI), questions concerning neurocompatibility still remain. In this study, we assessed potential adverse effects, as well as functional and histological impact of FS and EC in subtotal hemisection of the thoracic spinal cord (SC) in rats. METHODS: 124 male rats were randomly assigned to four main groups (n=31): Sham (SH), Lesion only (L), fibrin sealant (GFS) and equine collagen group (GEC). SH animals received laminectomy only; all other animals underwent subtotal lateral hemisection at T9. Treatment consisted of application of FS or EC into the lesion gap in GFS and GEC, which was left empty in L. GFS, GEC, L and SH were each further divided into 4 subgroups: One subgroup, consisting of 10 rats was subjected to behavioural and reflex testing before surgery and followed up on days 1,7, 14, 21, 28 post op and then sacrificed. Haemalaun or cresyl violet (CV) was used to identify neutrophils in parasagittal cord sections which were obtained on day 1 (n=7). Sections stained for quantification of microglia/macrophages using ED-1 on day 3 (n=7), day 7 (n=7) and day 28 (n=7 out of 10). Additionally, neural filament (NF) staining was chosen to detect axonal regeneration and the length of ingrowth into FS and EC, Luxol blue for myelination, Von Willebrand factor for vascularisation, and glial fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial scars on day 28. RESULTS: No adverse effects were observed in the treatment groups. Compared to L, GFS and GEC performed significantly better in the Basso, Beattie, Bresnahan (BBB) score and hopping responses. Proprioceptive placing was markedly improved in FS and EC compared to L. Axonal regrowth was found in GFS and GEC--the regrowth in the GFS was accompanied by myelination and vascularisation. Glial scarring occurred in all groups. Discussion Both biomatrices improved functional recovery compared to L and no adverse effects were perceived. |
|
|
Address |
Ludwig Boltzmann Institute of Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200-Vienna, Austria |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0940-2993 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17118635 |
Approved |
no |
|
|
Call Number |
|
Serial |
1852 |
|
Permanent link to this record |
|
|
|
|
Author |
Rosa, P.A.J.; Azevedo, A.M.; Aires-Barros, M.R. |
|
|
Title |
Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Journal of Chromatography. A |
Abbreviated Journal |
J Chromatogr A |
|
|
Volume |
1141 |
Issue |
1 |
Pages |
50-60 |
|
|
Keywords |
Analysis of Variance; Animals; Antibodies/*chemistry/*isolation & purification; Buffers; Chemical Fractionation/*methods; Horses; Humans; Hydrophobicity; Isoelectric Point; Models, Biological; Molecular Weight; Myoglobin/chemistry/isolation & purification; Osmolar Concentration; Phase Transition; Polyethylene Glycols; Serum Albumin/chemistry/isolation & purification; Sodium Chloride |
|
|
Abstract |
The partition of human antibodies in aqueous two-phase systems (ATPSs) of polyethylene glycol (PEG) and phosphate was systematically studied using first pure proteins systems and then an artificial mixture of proteins containing 1mg/ml human immunoglobulin G (IgG), 10mg/ml serum albumin and 2mg/ml myoglobin. Preliminary results obtained using pure proteins systems indicated that the PEG molecular weight and concentration, the pH value and the salts concentration had a pronounced effect on the partitioning behaviour of all proteins. For high ionic strengths and pH values higher than the isoelectric point (pI) of the contaminant proteins, IgG could be selectively recovered on the top phase. According to these results, a face centred composite design was performed in order to optimise the purification of IgG from the mixture of proteins. The optimal conditions for the isolation of IgG were observed for high concentrations of NaCl and low concentrations of both phase forming components. The best purification was achieved using an ATPS containing 8% (w/w) PEG 3350, 10% (w/w) phosphate pH 6 and 15% (w/w) NaCl. A recovery yield of 101+/-7%, a purity of 99+/-0% and a yield of native IgG of 97+/-4% were obtained. Back extraction studies of IgG to a new phosphate phase were performed and higher yields were obtained using 10% phosphate buffer at pH 6. The total extraction yield was 76% and the purity 100%. |
|
|
Address |
IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049-001 Lisbon, Portugal |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0021-9673 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17196214 |
Approved |
no |
|
|
Call Number |
|
Serial |
1842 |
|
Permanent link to this record |