|
Records |
Links |
|
Author |
Reynhout, I.C.; Cornelissen, J.J.L.M.; Nolte, R.J.M. |
|
|
Title |
Self-assembled architectures from biohybrid triblock copolymers |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Journal of the American Chemical Society |
Abbreviated Journal |
J Am Chem Soc |
|
|
Volume |
129 |
Issue |
8 |
Pages |
2327-2332 |
|
|
Keywords |
Horseradish Peroxidase/*chemistry; Micelles; Molecular Structure; Myoglobin/*chemistry; Particle Size; Polyethylene Glycols/*chemistry; Polymers/*chemical synthesis/chemistry; Polystyrenes/*chemistry; Surface-Active Agents/chemical synthesis/chemistry |
|
|
Abstract |
The synthesis and self-assembly behavior of biohybrid ABC triblock copolymers consisting of a synthetic diblock, polystyrene-b-polyethylene glycol (PSm-b-PEG113), where m is varied, and a hemeprotein, myoglobin (Mb) or horse radish peroxidase (HRP), is described. The synthetic diblock copolymer is first functionalized with the heme cofactor and subsequently reconstituted with the apoprotein or the apoenzyme to yield the protein-containing ABC triblock copolymer. The obtained amphiphilic block copolymers self-assemble in aqueous solution into a large variety of aggregate structures. Depending on the protein and the polystyrene block length, micellar rods, vesicles, toroids, figure eight structures, octopus structures, and spheres with a lamellar surface are formed. |
|
|
Address |
Institute for Molecules and Materials, Radboud University Nijmegen, Toernooiveld 1, 6525 ED Nijmegen, The Netherlands |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0002-7863 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17274615 |
Approved |
no |
|
|
Call Number |
|
Serial |
1832 |
|
Permanent link to this record |
|
|
|
|
Author |
Momozawa, Y.; Takeuchi, Y.; Tozaki, T.; Kikusui, T.; Hasegawa, T.; Raudsepp, T.; Chowdhary, B.P.; Kusunose, R.; Mori, Y. |
|
|
Title |
SNP detection and radiation hybrid mapping in horses of nine candidate genes for temperament |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
|
|
Volume |
38 |
Issue |
1 |
Pages |
81-83 |
|
|
Keywords |
Animals; *Behavior, Animal; Breeding; Horses/*genetics/physiology; *Polymorphism, Single Nucleotide; Radiation Hybrid Mapping; *Temperament |
|
|
Abstract |
|
|
|
Address |
Laboratory of Veterinary Ethology, The University of Tokyo, Tokyo 113-8657, Japan |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0268-9146 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17257195 |
Approved |
no |
|
|
Call Number |
|
Serial |
1834 |
|
Permanent link to this record |
|
|
|
|
Author |
Meral, Y.; Cakiroglu, D.; Sancak, A.A.; Cyftcy, G.; Karabacak, A. |
|
|
Title |
Relationships between serum serotonin and serum lipid levels, and aggression in horses |
Type |
Journal Article |
|
Year |
2007 |
Publication |
DTW. Deutsche Tierarztliche Wochenschrift |
Abbreviated Journal |
Dtsch Tierarztl Wochenschr |
|
|
Volume |
114 |
Issue |
1 |
Pages |
30-32 |
|
|
Keywords |
*Aggression; Animals; Behavior, Animal/*physiology; Case-Control Studies; Female; Horses/*blood; Lipids/*blood; Male; Serotonin/*blood |
|
|
Abstract |
Levels of serum serotonin and serum lipids--triglyceride, total cholesterol, low-density lipoprotein, high-density lipoprotein and very low-density lipoprotein, were determined in normal horses and horses diagnosed with aggression on the basis of a questionnaire survey. Blood serotonin levels in aggressive horses were found to be significantly lower than in non-aggressive horses (P < 0.01), but no association was found with respect to blood lipids. |
|
|
Address |
Department of Internal Diseases, Faculty of Veterinary Medicine, University of Ondokuz Mayis, Samsun, Turkey. ymeral@omu.edu.t |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0341-6593 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17252934 |
Approved |
no |
|
|
Call Number |
|
Serial |
1835 |
|
Permanent link to this record |
|
|
|
|
Author |
Krcmar, S.; Mikuska, A.; Merdic, E. |
|
|
Title |
Response of Tabanidae (Diptera) to different natural attractants |
Type |
Journal Article |
|
Year |
2006 |
Publication |
Journal of Vector Ecology : Journal of the Society for Vector Ecology |
Abbreviated Journal |
J Vector Ecol |
|
|
Volume |
31 |
Issue |
2 |
Pages |
262-265 |
|
|
Keywords |
Animals; Appetitive Behavior/*physiology; Cattle/urine; Diptera/*physiology; Female; Horses/urine; Insect Control/methods; Sheep/urine; Swine/urine; Urine/*physiology |
|
|
Abstract |
The response of female tabanids to natural attractants was studied in the Monjoros Forest along the Nature Park Kopacki rit in eastern Croatia. Tabanids were caught in canopy traps baited with either aged cow, horse, sheep, or pig urine and also in unbaited traps. Tabanids were collected in a significantly higher numbers in traps baited with natural attractants compared to unbaited traps. The number of females of Tabanus bromius, Tabanus maculicornis, Tabanus tergestinus, and Hybomitra bimaculata collected from canopy traps baited with cow urine and traps baited with other natural attractants differed significantly. Females of Haematopota pluvialis were also collected more frequently in canopy traps baited with aged cow urine than in those with aged horse urine, but this difference was not significant. However, the number of females of Haematopota pluvialis collected from canopy traps baited with other natural attractants (sheep and pig urine) differed significantly when compared with aged cow urine baited traps. Canopy traps baited with aged cow urine collected significantly more Tabanus sudeticus than did traps baited with aged pig urine. Finally, the aged cow urine baited canopy traps collected 51 times more tabanids than unbaited traps, while aged horse, aged sheep, and aged pig urine baited traps collected 36, 30, and 22 times as many tabanids, respectively, than unbaited traps. |
|
|
Address |
Department of Biology, JJ Strossmayer University, Lj. Gaja 6, HR-31000 Osijek, Croatia |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
1081-1710 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17249343 |
Approved |
no |
|
|
Call Number |
|
Serial |
1836 |
|
Permanent link to this record |
|
|
|
|
Author |
Mills, D.S. |
|
|
Title |
Comments about the importance of behaviour to equine clinicians |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
|
|
Volume |
39 |
Issue |
1 |
Pages |
95 |
|
|
Keywords |
Animals; *Behavior, Animal/physiology; *Clinical Competence; Horses/*physiology; Humans; Veterinarians/*psychology; Veterinary Medicine/*standards |
|
|
Abstract |
|
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0425-1644 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17228604 |
Approved |
no |
|
|
Call Number |
|
Serial |
1839 |
|
Permanent link to this record |
|
|
|
|
Author |
Machnik, M.; Hegger, I.; Kietzmann, M.; Thevis, M.; Guddat, S.; Schanzer, W. |
|
|
Title |
Pharmacokinetics of altrenogest in horses |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Journal of Veterinary Pharmacology and Therapeutics |
Abbreviated Journal |
J Vet Pharmacol Ther |
|
|
Volume |
30 |
Issue |
1 |
Pages |
86-90 |
|
|
Keywords |
Administration, Oral; Animals; Chromatography, Liquid/veterinary; Doping in Sports/prevention & control; Horses/*metabolism; Male; Mass Spectrometry/veterinary; Progesterone Congeners/administration & dosage/blood/*pharmacokinetics/urine; Reproducibility of Results; Substance Abuse Detection/veterinary; Trenbolone/administration & dosage/*analogs & derivatives/blood/pharmacokinetics/urine |
|
|
Abstract |
The Federation Equestre Internationale has permitted the use of altrenogest in mares for the control of oestrus. However, altrenogest is also suspicious to misuse in competition horses for its potential anabolic effects and suppression of typical male behaviour, and thus is a controlled drug. To investigate the pharmacokinetics of altrenogest in horses we conducted an elimination study. Five oral doses of 44 mug/kg altrenogest were administered to 10 horses at a dose interval of 24 h. Following administration blood and urine samples were collected at appropriate intervals. Altrenogest concentrations were measured by liquid chromatography-tandem mass spectrometry. The plasma levels of altrenogest reached maximal concentrations of 23-75 ng/mL. Baseline values were achieved within 3 days after the final administration. Urine peak concentrations of total altrenogest ranged from 823 to 3895 ng/mL. Twelve days after the final administration concentrations were below the limit of detection (ca 2 ng/mL). |
|
|
Address |
Institute of Biochemistry, German Sport University, Cologne, Germany. m.machnik@biochem.dshs-koeln.de |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0140-7783 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17217407 |
Approved |
no |
|
|
Call Number |
|
Serial |
1841 |
|
Permanent link to this record |
|
|
|
|
Author |
Rosa, P.A.J.; Azevedo, A.M.; Aires-Barros, M.R. |
|
|
Title |
Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Journal of Chromatography. A |
Abbreviated Journal |
J Chromatogr A |
|
|
Volume |
1141 |
Issue |
1 |
Pages |
50-60 |
|
|
Keywords |
Analysis of Variance; Animals; Antibodies/*chemistry/*isolation & purification; Buffers; Chemical Fractionation/*methods; Horses; Humans; Hydrophobicity; Isoelectric Point; Models, Biological; Molecular Weight; Myoglobin/chemistry/isolation & purification; Osmolar Concentration; Phase Transition; Polyethylene Glycols; Serum Albumin/chemistry/isolation & purification; Sodium Chloride |
|
|
Abstract |
The partition of human antibodies in aqueous two-phase systems (ATPSs) of polyethylene glycol (PEG) and phosphate was systematically studied using first pure proteins systems and then an artificial mixture of proteins containing 1mg/ml human immunoglobulin G (IgG), 10mg/ml serum albumin and 2mg/ml myoglobin. Preliminary results obtained using pure proteins systems indicated that the PEG molecular weight and concentration, the pH value and the salts concentration had a pronounced effect on the partitioning behaviour of all proteins. For high ionic strengths and pH values higher than the isoelectric point (pI) of the contaminant proteins, IgG could be selectively recovered on the top phase. According to these results, a face centred composite design was performed in order to optimise the purification of IgG from the mixture of proteins. The optimal conditions for the isolation of IgG were observed for high concentrations of NaCl and low concentrations of both phase forming components. The best purification was achieved using an ATPS containing 8% (w/w) PEG 3350, 10% (w/w) phosphate pH 6 and 15% (w/w) NaCl. A recovery yield of 101+/-7%, a purity of 99+/-0% and a yield of native IgG of 97+/-4% were obtained. Back extraction studies of IgG to a new phosphate phase were performed and higher yields were obtained using 10% phosphate buffer at pH 6. The total extraction yield was 76% and the purity 100%. |
|
|
Address |
IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049-001 Lisbon, Portugal |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0021-9673 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17196214 |
Approved |
no |
|
|
Call Number |
|
Serial |
1842 |
|
Permanent link to this record |
|
|
|
|
Author |
Naylor, J.M.; Poirier, K.L.; Hamilton, D.L.; Dowling, P.M. |
|
|
Title |
The effects of feeding and fasting on gastrointestinal sounds in adult horses |
Type |
Journal Article |
|
Year |
2006 |
Publication |
Journal of Veterinary Internal Medicine / American College of Veterinary Internal Medicine |
Abbreviated Journal |
J Vet Intern Med |
|
|
Volume |
20 |
Issue |
6 |
Pages |
1408-1413 |
|
|
Keywords |
Animals; Auscultation/methods/*veterinary; *Digestive Physiology; Fasting/*physiology; Gastrointestinal Motility/physiology; Gastrointestinal Transit/physiology; Horses/*physiology; Postprandial Period/*physiology; Sound |
|
|
Abstract |
The effect of changes in feed intake on auscultatable gastrointestinal sounds has not been systematically studied. Disagreement also is present in the literature about variation in sounds according to the quadrant of auscultation. Gastrointestinal sounds were recorded over the center of the left dorsal, left ventral, right ventral, and right dorsal quadrants and over the middle of the right abdominal flank. During 24 hours (n = 4) or 48 hours (n = 5) of fasting, there was a reduction in the intensity of gastrointestinal sounds as assessed by analysis of sound recordings. There was also a reduction in the number of mixing-like and propulsive-like sounds heard by 2 blinded observers. After refeeding, there was a marked increase in sound. Sound intensity varied among abdominal quadrants, but blinded observers did not notice significant differences in the number of mixing-like sounds. The left dorsal quadrant was quieter than others during fasting and refeeding. The right ventral quadrant appeared to be least affected by fasting, and sounds were louder over the right ventral and right middle quadrants than over the others. The blinded observers' perceptions of sound correlated poorly with one another and with objective measures of sound intensity. This experiment demonstrates the effectiveness of computerized analysis of abdominal sound in detecting a reduction in the intensity of gastrointestinal sounds during fasting and their return during refeeding. The left dorsal quadrant was quieter than other quadrants, likely because of its position over the small colon. There was considerable observer variation in the number of intestinal sounds heard. |
|
|
Address |
Department of Clinical Sciences, Ross University School of Veterinary Medicine, Basseterre, St Kitts, West Indies. jnaylor@rossvet.edu.kn |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0891-6640 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17186858 |
Approved |
no |
|
|
Call Number |
|
Serial |
1844 |
|
Permanent link to this record |
|
|
|
|
Author |
Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C. |
|
|
Title |
Molecular and cytogenetic paternity testing of a male offspring of a hinny |
Type |
Journal Article |
|
Year |
2006 |
Publication |
Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie |
Abbreviated Journal |
J Anim Breed Genet |
|
|
Volume |
123 |
Issue |
6 |
Pages |
403-405 |
|
|
Keywords |
Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal |
|
|
Abstract |
An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey. |
|
|
Address |
Equine Center, China Agricultural University, Beijing, China |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0931-2668 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17177697 |
Approved |
no |
|
|
Call Number |
|
Serial |
1846 |
|
Permanent link to this record |
|
|
|
|
Author |
Petter-Puchner, A.H.; Froetscher, W.; Krametter-Froetscher, R.; Lorinson, D.; Redl, H.; van Griensven, M. |
|
|
Title |
The long-term neurocompatibility of human fibrin sealant and equine collagen as biomatrices in experimental spinal cord injury |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft fur Toxikologische Pathologie |
Abbreviated Journal |
Exp Toxicol Pathol |
|
|
Volume |
58 |
Issue |
4 |
Pages |
237-245 |
|
|
Keywords |
Animals; Axotomy; Biocompatible Materials/*therapeutic use; Collagen/*therapeutic use; Fibrin Tissue Adhesive/*therapeutic use; Horses; Humans; Immunohistochemistry; Male; Motor Activity/physiology; Nerve Regeneration/*physiology; Rats; Recovery of Function; Spinal Cord/pathology/physiology; Spinal Cord Injuries/pathology/*therapy; Thoracic Vertebrae |
|
|
Abstract |
INTRODUCTION: While fibrin sealant (FS) and equine collagen (EC) have been used as scaffold materials in experimental spinal cord injury (SCI), questions concerning neurocompatibility still remain. In this study, we assessed potential adverse effects, as well as functional and histological impact of FS and EC in subtotal hemisection of the thoracic spinal cord (SC) in rats. METHODS: 124 male rats were randomly assigned to four main groups (n=31): Sham (SH), Lesion only (L), fibrin sealant (GFS) and equine collagen group (GEC). SH animals received laminectomy only; all other animals underwent subtotal lateral hemisection at T9. Treatment consisted of application of FS or EC into the lesion gap in GFS and GEC, which was left empty in L. GFS, GEC, L and SH were each further divided into 4 subgroups: One subgroup, consisting of 10 rats was subjected to behavioural and reflex testing before surgery and followed up on days 1,7, 14, 21, 28 post op and then sacrificed. Haemalaun or cresyl violet (CV) was used to identify neutrophils in parasagittal cord sections which were obtained on day 1 (n=7). Sections stained for quantification of microglia/macrophages using ED-1 on day 3 (n=7), day 7 (n=7) and day 28 (n=7 out of 10). Additionally, neural filament (NF) staining was chosen to detect axonal regeneration and the length of ingrowth into FS and EC, Luxol blue for myelination, Von Willebrand factor for vascularisation, and glial fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial scars on day 28. RESULTS: No adverse effects were observed in the treatment groups. Compared to L, GFS and GEC performed significantly better in the Basso, Beattie, Bresnahan (BBB) score and hopping responses. Proprioceptive placing was markedly improved in FS and EC compared to L. Axonal regrowth was found in GFS and GEC--the regrowth in the GFS was accompanied by myelination and vascularisation. Glial scarring occurred in all groups. Discussion Both biomatrices improved functional recovery compared to L and no adverse effects were perceived. |
|
|
Address |
Ludwig Boltzmann Institute of Experimental and Clinical Traumatology, Donaueschingenstrasse 13, 1200-Vienna, Austria |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0940-2993 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17118635 |
Approved |
no |
|
|
Call Number |
|
Serial |
1852 |
|
Permanent link to this record |