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Author Erber, R.; Wulf, M.; Aurich, J.; Becker-Birck, M.; Rose-Meierhöfer, S.; Möstl, E.; Hoffmann, G.; Aurich, C.
Title Physiological stress parameters in sport horse mares transferred from group housing to individual stabling Type (up) Conference Article
Year 2012 Publication Proceedings of the 2. International Equine Science Meeting Abbreviated Journal Proc. 2. Int. Equine. Sci. Mtg
Volume in press Issue Pages
Keywords horse, isolation, cortisol, heart rate, locomotion
Abstract Initial equestrian training and especially first mounting of a rider are stressful challenges for young horses (1). Most young horses are raised in groups but, in association with equestrian training, they are commonly transferred to individual stabling in loose boxes. Although, in most stables, visual contact with horses in adjacent boxes is possible, separation from the herd might be an additional stressor. We have studied physiological stress parameters, in 3-year-old sport horse mares (n=8), transferred from a group stable with access to a paddock to individual boxes without paddock. Once stabled in the individual boxes, mares underwent a standard training for young horses. Horses had been accustomed to lunging and tolerating a rider on their back several weeks before the study. Mares were studied from 5 days before to 5 days after changing the stable. Cortisol concentration in saliva, locomotion activity (ALT pedometers), heart rate (HR) and HR variability (RMSSD: root mean square of successive beat-to-beat intervals) were determined. We hypothesized that the change of the stable increases cortisol release and is associated with changes in HR and RMSSD and reduced locomotion. Before mares were moved to individual boxes, cortisol concentration showed a pronounced diurnal rhythm with values around 0.6 ng/ ml in the morning and a continuous decrease throughout the day. When the mares were moved to individual boxes, cortisol concentration increased to 1.8±0.2 ng/ml and did not return to baseline values within 6 h (p<0.05 over time). On subsequent days, a diurnal rhythm was re-established but shifted to a higher level than before. Locomotion activity determined by ALT pedometers was increased for some minutes only after mares has been placed in individual boxes but was only slightly higher than during the time mares spent with the group in a paddock. On days 2-5 in individual boxes, locomotion activity was reduced compared to the group stable. HR increased and the HRV variable RMSSD decreased when mares were separated. In conclusion, separating horses during initial training from their group is an additional stressor, although the stress is less pronounced than induced by other social challenges, e.g. weaning of foals (2). When stabled in individual boxes, mares move less than when kept as a group. Horses kept in a group thus appear to exercise themselves freely, such an effect is absent when the animals are kept individually.
Address
Corporate Author Erber, R. Thesis
Publisher Xenophon Publishing Place of Publication Wald Editor Krueger, K.
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 978-3-9808134-26 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5542
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Author Alexander, F.; Davies, M.E.; Muir, A.R.
Title Bacteriophage-like particles in the large intestine of the horse Type (up) Journal Article
Year 1970 Publication Research in veterinary science Abbreviated Journal Res Vet Sci
Volume 11 Issue 6 Pages 592-593
Keywords Animals; Bacteriophages/*isolation & purification; Cecum/microbiology; Colon/microbiology
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0034-5288 ISBN Medium
Area Expedition Conference
Notes PMID:5498578 Approved no
Call Number refbase @ user @ Serial 114
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Author Assersohn, C.; Whiten, A.; Kiwede, Z.T.; Tinka, J.; Karamagi, J.
Title Use of leaves to inspect ectoparasites in wild chimpanzees: a third cultural variant? Type (up) Journal Article
Year 2004 Publication Primates Abbreviated Journal Primates
Volume 45 Issue 4 Pages 255-258
Keywords Animals; Animals, Wild/physiology; Ape Diseases/*parasitology; Behavior, Animal/*physiology; Ectoparasitic Infestations/parasitology/*veterinary; Female; Grooming/*physiology; Male; Pan troglodytes/*physiology; *Plant Leaves; Protozoa/*isolation & purification; Uganda
Abstract We report 26 cases of using leaves as tools with which wild chimpanzees (Pan troglodytes schweinfurthii) in the Sonso community, Budongo Forest, Uganda, appeared to inspect objects removed during grooming. Careful removal of potential ectoparasites and delicate lip or manual placement on leaves followed by intense visual examination characterised this behaviour. It appears to be done to judge whether either ingestion or discarding is most appropriate, the former occurring in most cases. This behaviour may represent a third variant of ectoparasite handling, different from those described at Tai and Gombe, yet sharing features with the latter. These two East African techniques may thus have evolved from leaf grooming.
Address Scottish Primate Research Group, School of Psychology, University of St Andrews, St Andrews, KY16 9JU, Fife, UK
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0032-8332 ISBN Medium
Area Expedition Conference
Notes PMID:15179558 Approved no
Call Number refbase @ user @ Serial 733
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Author Rosa, P.A.J.; Azevedo, A.M.; Aires-Barros, M.R.
Title Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies Type (up) Journal Article
Year 2007 Publication Journal of Chromatography. A Abbreviated Journal J Chromatogr A
Volume 1141 Issue 1 Pages 50-60
Keywords Analysis of Variance; Animals; Antibodies/*chemistry/*isolation & purification; Buffers; Chemical Fractionation/*methods; Horses; Humans; Hydrophobicity; Isoelectric Point; Models, Biological; Molecular Weight; Myoglobin/chemistry/isolation & purification; Osmolar Concentration; Phase Transition; Polyethylene Glycols; Serum Albumin/chemistry/isolation & purification; Sodium Chloride
Abstract The partition of human antibodies in aqueous two-phase systems (ATPSs) of polyethylene glycol (PEG) and phosphate was systematically studied using first pure proteins systems and then an artificial mixture of proteins containing 1mg/ml human immunoglobulin G (IgG), 10mg/ml serum albumin and 2mg/ml myoglobin. Preliminary results obtained using pure proteins systems indicated that the PEG molecular weight and concentration, the pH value and the salts concentration had a pronounced effect on the partitioning behaviour of all proteins. For high ionic strengths and pH values higher than the isoelectric point (pI) of the contaminant proteins, IgG could be selectively recovered on the top phase. According to these results, a face centred composite design was performed in order to optimise the purification of IgG from the mixture of proteins. The optimal conditions for the isolation of IgG were observed for high concentrations of NaCl and low concentrations of both phase forming components. The best purification was achieved using an ATPS containing 8% (w/w) PEG 3350, 10% (w/w) phosphate pH 6 and 15% (w/w) NaCl. A recovery yield of 101+/-7%, a purity of 99+/-0% and a yield of native IgG of 97+/-4% were obtained. Back extraction studies of IgG to a new phosphate phase were performed and higher yields were obtained using 10% phosphate buffer at pH 6. The total extraction yield was 76% and the purity 100%.
Address IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049-001 Lisbon, Portugal
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0021-9673 ISBN Medium
Area Expedition Conference
Notes PMID:17196214 Approved no
Call Number Serial 1842
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Author Blunden, A.S.; Smith, K.C.; Whitwell, K.E.; Dunn, K.A.
Title Systemic infection by equid herpesvirus-1 in a Grevy's zebra stallion (Equus grevyi) with particular reference to genital pathology Type (up) Journal Article
Year 1998 Publication Journal of Comparative Pathology Abbreviated Journal J Comp Pathol
Volume 119 Issue 4 Pages 485-493
Keywords Animals; Animals, Zoo; Epididymis/pathology/virology; Equidae/*virology; Herpesviridae Infections/diagnosis/pathology/*veterinary; Herpesvirus 1, Equid/isolation & purification/*pathogenicity; Lymph Nodes/pathology/virology; Male; Nasal Mucosa/pathology/virology; Pulmonary Edema/pathology; Spleen/virology; Testis/*pathology/virology
Abstract A severe multi-systemic form of equid herpesvirus-1 infection is described in an adult zebra stallion. There was multifocal necrotizing rhinitis, marked hydrothorax and pulmonary oedema, with viral antigen expression in degenerating epithelial cells, local endothelial cells and intravascular leucocytes of the nasal mucosa and lung. Specific localization of EHV-1 infection was seen in the testes and epididymides, including infection of Leydig cells and germinal epithelium, which would have facilitated venereal shedding of virus in life. The case provided a unique opportunity to study hitherto undescribed aspects of the pathogenesis of naturally occurring EHV-1 infection in the male equine genital tract. Restriction digests of the isolate demonstrated a pattern similar to that of EHV-1 isolates previously recovered from aborted zebra and onager fetuses.
Address Animal Health Trust Centre for Preventive Medicine, Newmarket, Suffolk, UK
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0021-9975 ISBN Medium
Area Expedition Conference
Notes PMID:9839210 Approved no
Call Number Equine Behaviour @ team @ Serial 2239
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Author Dvoinos, G.M.; Kharchenko, V.A.; Zviagnitsova, N.S.
Title The characteristics of the helminth community in the Turkmen kulan (Equus hemionus) Type (up) Journal Article
Year 1992 Publication Parazitologiia Abbreviated Journal Parazitologiia
Volume 26 Issue 3 Pages 246-251
Keywords Animals; Female; Helminthiasis/parasitology; Helminthiasis, Animal; Helminths/classification/*isolation & purification; Male; Perissodactyla/*parasitology; Turkmenistan; Ukraine
Abstract The helminth fauna of 24 kulans from Askaniya-Nova and Badkhyz was studied. 42 species of helminths were found, 34 of which belong to strongylids. The helminth species composition of kulan is similar to that of other species of horses. This is a result of an intensive parasite exchange in the historical past when numerous populations of different Equidae species made long seasonal migrations over steppe inter-river lands of Asia and grazed for some time on common pastures.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Russian Summary Language Original Title K kharakteristike soobshchestva gel'mintov turkmenskogo kulana (Equus hemionus)
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1847 ISBN Medium
Area Expedition Conference
Notes PMID:1408368 Approved no
Call Number Equine Behaviour @ team @ Serial 2285
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Author Sinclair, M.; Buhrmann, G.; Gummow, B.
Title An epidemiological investigation of the African horsesickness outbreak in the Western Cape Province of South Africa in 2004 and its relevance to the current equine export protocol Type (up) Journal Article
Year 2006 Publication Journal of the South African Veterinary Association Abbreviated Journal J S Afr Vet Assoc
Volume 77 Issue 4 Pages 191-196
Keywords African Horse Sickness/diagnosis/*epidemiology; African horse sickness virus/*isolation & purification; Animals; Ceratopogonidae/virology; Diagnosis, Differential; Disease Outbreaks/*veterinary; Female; Horses; Insect Vectors/virology; Male; Prevalence; Retrospective Studies; Sentinel Surveillance; South Africa/epidemiology; Viral Vaccines/administration & dosage
Abstract African Horsesickness (AHS) is a controlled disease in South Africa. The country is divided into an infected area and a control area. An outbreak of AHS in the control area can result in a ban of exports for at least 2 years. A retrospective epidemiological study was carried out on data collected during the 2004 AHS outbreak in the surveillance zone of the AHS control area in the Western Cape Province. The objective of this study was to describe the 2004 outbreak and compare it with the 1999 AHS outbreak in the same area. As part of the investigation, a questionnaire survey was conducted in the 30 km radius surrounding the index case. Spatial, temporal and population patterns for the outbreak are described. The investigation found that the outbreak occurred before any significant rainfall and that the main AHS vector (Culicoides imicola) was present in abundance during the outbreak. Furthermore, 63% of cases occurred at temperatures < or = 15 degrees C, the Eerste River Valley was a high risk area, only 17% of owners used vector protection as a control measure and 70% of horses in the outbreak area were protected by means of vaccination at the start of the outbreak. The study revealed that the current AHS control measures do not function optimally because of the high percentage of vaccinated horses in the surveillance zone, which results in insufficient sentinel animals and the consequent failure of the early warning system. Alternative options for control that allow continued export are discussed in the paper.
Address State Veterinarian Epidemiology, Elsenburg, South Africa. marnas@elsenburg.com
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1019-9128 ISBN Medium
Area Expedition Conference
Notes PMID:17458343 Approved no
Call Number Equine Behaviour @ team @ Serial 2354
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Author Venter, G.J.; Koekemoer, J.J.O.; Paweska, J.T.
Title Investigations on outbreaks of African horse sickness in the surveillance zone in South Africa Type (up) Journal Article
Year 2006 Publication Revue Scientifique et Technique (International Office of Epizootics) Abbreviated Journal Rev Sci Tech
Volume 25 Issue 3 Pages 1097-1109
Keywords African Horse Sickness/*epidemiology; African horse sickness virus/genetics/*isolation & purification; Animals; Ceratopogonidae/*virology; Disease Outbreaks/*veterinary; Horses; Insect Vectors/*virology; Prevalence; Sentinel Surveillance/veterinary; South Africa/epidemiology
Abstract Confirmed outbreaks of African horse sickness (AHS) occurred in the surveillance zone of the Western Cape in 1999 and 2004, both of which led to a two-year suspension on the export of horses. Light trap surveys in the outbreak areas showed that known vector competent Culicoides species, notably C. imicola, were abundant and present in numbers equal to those in the traditional AHS endemic areas. Isolations of AHS virus serotypes 1 and 7, equine encephalosis virus, and bluetongue virus from field-collected C. imicola in the surveillance zone demonstrated that this species was highly competent and could transmit viruses belonging to different serogroups of the Orbivirus genus. Molecular identification of recovered virus isolates indicated that at least two incursions of AHS into the surveillance zone had taken place in 2004. The designation of an AHS-free zone in the Western Cape remains controversial since it can be easily compromised, as evidenced by the two recent outbreaks. In light of the results reported in the present study, the policy of maintaining a large population of unvaccinated horses in the surveillance zone should be reconsidered, as it leaves them vulnerable to infection with AHS virus, which is the most pathogenic of all equine viruses.
Address Agricultural Research Council-Onderstepoort Veterinary Institute, Private Bag X5, Onderstepoort, 0110 South Africa
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0253-1933 ISBN Medium
Area Expedition Conference
Notes PMID:17361773 Approved no
Call Number Equine Behaviour @ team @ Serial 2355
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Author Milinovich, G.J.; Trott, D.J.; Burrell, P.C.; van Eps, A.W.; Thoefner, M.B.; Blackall, L.L.; Al Jassim, R.A.M.; Morton, J.M.; Pollitt, C.C.
Title Changes in equine hindgut bacterial populations during oligofructose-induced laminitis Type (up) Journal Article
Year 2006 Publication Environmental Microbiology Abbreviated Journal Environ Microbiol
Volume 8 Issue 5 Pages 885-898
Keywords Animal Feed; Animals; Bacteria/classification/*isolation & purification; DNA, Bacterial/analysis; Disease Models, Animal; Feces/microbiology; Foot Diseases/etiology/microbiology/*veterinary; Horse Diseases/*etiology/metabolism/microbiology; Horses; In Situ Hybridization, Fluorescence; Intestines/*microbiology; Oligosaccharides/*administration & dosage/*metabolism; Phylogeny; Polymerase Chain Reaction; RNA, Bacterial/analysis; RNA, Ribosomal, 16S/analysis
Abstract In the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. The objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. Laminitis was induced in five horses by administration of oligofructose. Faecal specimens were collected at 8 h intervals from 72 h before to 72 h after the administration of oligofructose. Hindgut microbiota able to utilize oligofructose were enumerated throughout the course of the experiment using habitat-simulating medium. Isolates were collected and representatives identified by 16S rRNA gene sequencing. The majority of these isolates collected belonged to the genus Streptococcus, 91% of which were identified as being most closely related to Streptococcus infantarius ssp. coli. Furthermore, S. infantarius ssp. coli was the predominant oligofructose-utilizing organism isolated before the onset of lameness. Fluorescence in situ hybridization probes developed to specifically target the isolated Streptococcus spp. demonstrated marked population increases between 8 and 16 h post oligofructose administration. This was followed by a rapid population decline which corresponded with a sharp decline in faecal pH and subsequently lameness at 24-32 h post oligofructose administration. This research suggests that streptococci within the Streptococcus bovis/equinus complex may be involved in the series of events which precede the onset of laminitis in the horse.
Address Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Queensland, Australia. g.milinovich@uq.edu.au
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1462-2912 ISBN Medium
Area Expedition Conference
Notes PMID:16623745 Approved no
Call Number Equine Behaviour @ team @ Serial 2625
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Author Muscatello, G.; Anderson, G.A.; Gilkerson, J.R.; Browning, G.F.
Title Associations between the ecology of virulent Rhodococcus equi and the epidemiology of R. equi pneumonia on Australian thoroughbred farms Type (up) Journal Article
Year 2006 Publication Applied and Environmental Microbiology Abbreviated Journal Appl Environ Microbiol
Volume 72 Issue 9 Pages 6152-6160
Keywords Actinomycetales Infections/epidemiology/microbiology/*veterinary; Air Microbiology; Animal Husbandry; Animals; Animals, Newborn; Australia/epidemiology; Colony Count, Microbial; DNA, Bacterial/genetics; Ecosystem; Horse Diseases/epidemiology/*microbiology; Horses; Pneumonia, Bacterial/epidemiology/microbiology/*veterinary; Rhodococcus equi/genetics/isolation & purification/*pathogenicity; Soil Microbiology; Virulence
Abstract The ecology of virulent strains of Rhodococcus equi on horse farms is likely to influence the prevalence and severity of R. equi pneumonia in foals. This study examined the association between the ecology of virulent R. equi and the epidemiology of R. equi pneumonia by collecting air and soil samples over two breeding seasons (28 farm-year combinations) on Thoroughbred breeding farms with different reported prevalences of R. equi pneumonia. Colony blotting and DNA hybridization were used to detect and measure concentrations of virulent R. equi. The prevalence of R. equi pneumonia was associated with the airborne burden of virulent R. equi (both the concentration and the proportion of R. equi bacteria that were virulent) but was not associated with the burden of virulent R. equi in the soil. Univariable screening and multivariable model building were used to evaluate the effect of environmental and management factors on virulent R. equi burdens. Lower soil moisture concentrations and lower pasture heights were significantly associated with elevated airborne concentrations of virulent R. equi, as were the holding pens and lanes, which typically were sandy, dry, and devoid of pasture cover. Few variables appeared to influence concentrations of virulent R. equi in soil. Acidic soil conditions may have contributed to an elevated proportion of virulent strains within the R. equi population. Environmental management strategies that aim to reduce the level of exposure of susceptible foals to airborne virulent R. equi are most likely to reduce the impact of R. equi pneumonia on endemically affected farms.
Address School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. mug@unimelb.edu.au
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0099-2240 ISBN Medium
Area Expedition Conference
Notes PMID:16957241 Approved no
Call Number Equine Behaviour @ team @ Serial 2622
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