Records |
Author |
Bartosova, J.; Dvorakova, R.; Vancatova, M.; Svobodova, I. |
Title |
Comprehension of human pointing gesture in domestic horses: Effect of training method |
Type |
Conference Article |
Year |
2008 |
Publication |
IESM 2008 |
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Volume |
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Issue |
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Pages |
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Keywords |
Human-horse communication, Pointing, Training methods, Horsemanship |
Abstract |
Horses have been considered to rely on human gesticular cues (McKinley and Sambrook 2000, Anim Cogn 3:13-22; and recently Maros et al. 2008, Anim Cogn 11:457-466), however large variability among individuals tested in two-ways object choice tasks was found. Part of the horses in those studies (40 and 26 %, respectively) even failed to pass adequately through the training session which preceded the testing phase and served to learn a horse to carry out a task. Therefore, we alternated the experimental design designed by McKinley and Sambrook (reduced number of testing trials to 10 per horse to keep its attention, applied just one, a dynamic-sustained pointing cue with touching the bucket, etc.), and tested an effect of training method, sex, age, and learning on proportion of correct choices. We hypothesised, that horses trained by “traditional” method (TTM) will get lower score than those experienced with “horsemanship-based” methods (HTM), being characterized by closer and more frequent human-horse contact and also extended exercising “from the ground” with frequent using of arms cues. Despite simplification of the methods, only about 60 % of tested horses passed through the training phase (i.e., learned to come to and upturn the bucket with hidden treat). Successful completion of training phase was reached regardless of age or sex of a horse, but by the training method; HTM horses ran better compared to TTM ones. No significant effect of age, sex, or learning (i.e., trial order within all 10), and training method as well was found on proportion of correct trials in the testing phase. Horses made a correct choice in more than 70% of trials. Individual scores ranged from 50 to 100 %. In conclusion, horses showed high level of comprehension of human pointing gesture, regardless of their sex or age. No effects of training method or learning process within a test suggest low impact of handling and learning on the level of comprehension at least of the most vivid human pointing gesture. Horses trained by methods based on “natural human-horse communication” did enhance cooperation with people. |
Address |
Department of Ethology, Institute of Animal Science, Pratelstvi 815, CZ-104 00 Praha Uhrineves, Czech Republic |
Corporate Author |
Bartosova, J. |
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IESM 2008 |
Notes |
Talk 15 min IESM 2008 |
Approved |
yes |
Call Number |
Equine Behaviour @ team @ |
Serial |
4464 |
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Author |
Heistermann, M.; Palme, R.; Ganswindt, A. |
Title |
Comparison of different enzyme-immunoassays for assessment of adrenocortical activity in primates based on fecal analysis |
Type |
Journal Article |
Year |
2006 |
Publication |
American journal of primatology |
Abbreviated Journal |
Am. J. Primatol. |
Volume |
68 |
Issue |
3 |
Pages |
257-273 |
Keywords |
11-Hydroxycorticosteroids/*analysis; Adrenocorticotropic Hormone/pharmacology; Anesthesia; Animals; Corticosterone/analysis; Feces/*chemistry; Glucocorticoids/*analysis; Haplorhini/*metabolism; Hydrocortisone/analysis; Hypothalamo-Hypophyseal System/drug effects/physiology; Immunoenzyme Techniques/*methods; Pituitary-Adrenal System/drug effects/physiology; Species Specificity |
Abstract |
Most studies published to date that used fecal glucocorticoid measurements to assess adrenocortical activity in primate (and many nonprimate) species applied a specific cortisol or corticosterone assay. However, since these native glucocorticoids are virtually absent in the feces of most vertebrates, including primates, the validity of this approach has recently been questioned. Therefore, the overall aim of the present study was to assess the validity of four enzyme-immunoassays (EIAs) using antibodies raised against cortisol, corticosterone, and reduced cortisol metabolites (two group-specific antibodies) for assessing adrenocortical activity using fecal glucocorticoid metabolite (GCM) measurements in selected primate species (marmoset, long-tailed macaque, Barbary macaque, chimpanzee, and gorilla). Using physiological stimulation of the hypothalamo-pituitary-adrenocortical (HPA) axis by administering exogenous ACTH or anesthesia, we demonstrated that at least two assays detected the predicted increase in fecal GCM levels in response to treatment in each species. However, the magnitude of response varied between assays and species, and no one assay was applicable to all species. While the corticosterone assay generally was of only limited suitability for assessing glucocorticoid output, the specific cortisol assay was valuable for those species that (according to high-performance liquid chromatography (HPLC) analysis data) excreted clearly detectable amounts of authentic cortisol into the feces. In contrast, in species in which cortisol was virtually absent in the feces, group-specific assays provided a much stronger signal, and these assays also performed well in the other primate species tested (except the marmoset). Collectively, the data suggest that the reliability of a given fecal glucocorticoid assay in reflecting activity of the HPA axis in primates clearly depends on the species in question. Although to date there is no single assay system that can be used successfully across species, our data suggest that group-specific assays have a high potential for cross-species application. Nevertheless, regardless of which GC antibody is chosen, our study clearly reinforces the necessity of appropriately validating the respective assay system before it is used. |
Address |
Department of Reproductive Biology, German Primate Center, Gottingen, Germany. mheiste@gwdg.de |
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ISSN |
0275-2565 |
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Notes |
PMID:16477600 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4078 |
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Author |
Bottoms, G.D.; Roesel, O.F.; Rausch, F.D.; Akins, E.L. |
Title |
Circadian variation in plasma cortisol and corticosterone in pigs and mares |
Type |
Journal Article |
Year |
1972 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
Volume |
33 |
Issue |
4 |
Pages |
785-790 |
Keywords |
Animals; *Circadian Rhythm; Corticosterone/*blood; Female; Horses/*metabolism; Hydrocortisone/*blood; Methods; Swine/*metabolism; Time Factors |
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English |
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ISSN |
0002-9645 |
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Notes |
PMID:5017871 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4074 |
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Author |
Kirkpatrick, J.F.; Turner, J.W.J.; Liu, I.K.; Fayrer-Hosken, R.; Rutberg, A.T. |
Title |
Case studies in wildlife immunocontraception: wild and feral equids and white-tailed deer |
Type |
Journal Article |
Year |
1997 |
Publication |
Reproduction, fertility, and development |
Abbreviated Journal |
Reprod Fertil Dev |
Volume |
9 |
Issue |
1 |
Pages |
105-110 |
Keywords |
Animals; Antigens/immunology; Contraception, Immunologic/*veterinary; *Deer; *Equidae; Female; Immunization, Secondary; Pest Control/*methods; Pregnancy; Swine; Vaccines/administration & dosage; Zona Pellucida/immunology |
Abstract |
Non-lethal management methods are required for wild equids that are protected by law and for deer inhabiting areas where lethal controls are not legal or safe. Single or multiple inoculations of porcine zona pellucida (PZP) vaccine have been delivered to wild horses and deer by means of darts. Contraceptive efficacy in horses after two inoculations ranged from 90% to 100%, and after a single inoculation ranged from 19% to 28%. Mares given a controlled-release form of the vaccine had foaling rates ranging from 7% to 20%. No detectable changes in social organization or behaviours among treated horses occurred. Contraceptive effects were reversible after 4 consecutive years of treatment but 5-7 years of treatment resulted in ovulation failure and decreased urinary oestrogen concentrations. Among deer, two inoculations were 70-100% effective in preventing fawns, but one inoculation yielded a contraceptive efficacy of < or = 20%, with pregnancies occurring late in the breeding season; a single annual booster inoculation reduced fertility to 20% in the second year. Energy costs of extended breeding seasons were less than those resulting from pregnancy. After two years of treatment, ovaries appeared normal. These studies suggest that PZP immunocontraception can be successfully applied to certain free-roaming populations of wild horses and deer. |
Address |
ZooMontana, Billings 59108, USA |
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English |
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Edition |
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ISSN |
1031-3613 |
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Area |
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Notes |
PMID:9109199 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
143 |
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Author |
Waran, N.K. |
Title |
Can studies of feral horse behaviour be used for assessing domestic horse welfare? |
Type |
|
Year |
1997 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
Volume |
29 |
Issue |
4 |
Pages |
249-251 |
Keywords |
Animal Husbandry/methods; *Animal Welfare; Animals; Animals, Domestic; Animals, Wild; *Behavior, Animal; Horses/*psychology; Social Behavior |
Abstract |
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Place of Publication |
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English |
Summary Language |
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ISSN |
0425-1644 |
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Notes |
PMID:15338901 |
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no |
Call Number |
refbase @ user @ |
Serial |
1936 |
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Author |
Grogan, E.H.; McDonnell, S.M. |
Title |
Behavioral responses to two intranasal vaccine applicators in horses and ponies |
Type |
|
Year |
2005 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
Volume |
226 |
Issue |
10 |
Pages |
1689-1693 |
Keywords |
Administration, Intranasal; Animals; *Behavior, Animal; Female; Horses/physiology/*psychology; Immunization/instrumentation/methods/*veterinary; Male; Patient Compliance/psychology; Physical Examination/psychology/*veterinary; Vaccines/*administration & dosage; Videotape Recording |
Abstract |
OBJECTIVE: To evaluate behavioral compliance of horses and ponies with simulated intranasal vaccination and assess development of generalized aversion to veterinary manipulations. DESIGN: Clinical trial. ANIMALS: 28 light horse mares, 3 pony geldings, 2 light horse stallions, and 3 pony stallions that had a history of compliance with veterinary procedures. PROCEDURE: Behavioral compliance with 2 intranasal vaccine applicators was assessed. Compliance with standard physical examination procedures was assessed before and after a single experience with either of the applicators or a control manipulation to evaluate development of generalized aversion to veterinary manipulation. RESULTS: In all 30 horses, simulated intranasal vaccination or the control manipulation could be performed without problematic avoidance behavior, and simulated intranasal vaccination did not have any significant effect on duration of or compliance with a standardized physical examination that included manipulation of the ears, nose, and mouth. Results were similar for the 2 intranasal vaccine applicators, and no difference in compliance was seen between horses in which warm versus cold applicators were used. For 3 of the 6 ponies, substantial avoidance behavior was observed in association with simulated intranasal vaccination, and compliance with physical examination procedures decreased after simulated intranasal vaccination. CONCLUSIONS AND CLINICAL RELEVANCE: Although some compliance problems were seen with ponies, neither problems with compliance with simulated intranasal vaccination nor adverse effects on subsequent physical examination were identified in any of the horses. Further study is needed to understand factors involved in practitioner reports of aversion developing in association with intranasal vaccination. |
Address |
Equine Behavior Laboratory, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA |
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English |
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Edition |
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ISSN |
0003-1488 |
ISBN |
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Area |
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Notes |
PMID:15906570 |
Approved |
no |
Call Number |
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Serial |
1890 |
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Author |
Scheibe, K.M.; Gromann, C. |
Title |
Application testing of a new three-dimensional acceleration measuring system with wireless data transfer (WAS) for behavior analysis |
Type |
|
Year |
2006 |
Publication |
Behavior research methods |
Abbreviated Journal |
Behav Res Methods |
Volume |
38 |
Issue |
3 |
Pages |
427-433 |
Keywords |
Acceleration; Animals; *Behavior, Animal; Cattle; Cattle Diseases/*diagnosis; Computer Communication Networks/*instrumentation; Forelimb/physiopathology; Fractals; Hindlimb/physiopathology; Horse Diseases/*diagnosis; Horses; Imaging, Three-Dimensional/instrumentation/methods/veterinary; Lameness, Animal/*diagnosis; Monitoring, Ambulatory/instrumentation/*methods; Motor Activity; Movement; Pattern Recognition, Automated/methods |
Abstract |
A wireless acceleration measurement system was applied to free-moving cows and horses. Sensors were available as a collar and a flat box for measuring leg or trunk movements. Results were transmitted simultaneously by radio or stored in an 8-MB internal memory. As analytical procedures, frequency distributions with standard deviations, spectral analyses, and fractal analyses were applied. Bymeans of the collar sensor, basic behavior patterns (standing, grazing, walking, ruminating, drinking, and hay uptake) could be identified in cows. Lameness could be detected in cows and horses by means of the leg sensor. The portion of basic and harmonic spectral components was reduced; the fractal dimension was reduced. The system can be used for the detection and analysis of even small movements of free-moving humans or animals over several hours. It is convenient for the analysis of basic behaviors, emotional reactions, or events causing flight or fright or for comparing different housing elements, such as floors or fences. |
Address |
Institute for Zoo and Wildlife Research, Berlin, Germany. kscheibe@izw-berlin.de |
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English |
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ISSN |
1554-351X |
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Notes |
PMID:17186752 |
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no |
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Serial |
1775 |
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Author |
Rosa, P.A.J.; Azevedo, A.M.; Aires-Barros, M.R. |
Title |
Application of central composite design to the optimisation of aqueous two-phase extraction of human antibodies |
Type |
Journal Article |
Year |
2007 |
Publication |
Journal of Chromatography. A |
Abbreviated Journal |
J Chromatogr A |
Volume |
1141 |
Issue |
1 |
Pages |
50-60 |
Keywords |
Analysis of Variance; Animals; Antibodies/*chemistry/*isolation & purification; Buffers; Chemical Fractionation/*methods; Horses; Humans; Hydrophobicity; Isoelectric Point; Models, Biological; Molecular Weight; Myoglobin/chemistry/isolation & purification; Osmolar Concentration; Phase Transition; Polyethylene Glycols; Serum Albumin/chemistry/isolation & purification; Sodium Chloride |
Abstract |
The partition of human antibodies in aqueous two-phase systems (ATPSs) of polyethylene glycol (PEG) and phosphate was systematically studied using first pure proteins systems and then an artificial mixture of proteins containing 1mg/ml human immunoglobulin G (IgG), 10mg/ml serum albumin and 2mg/ml myoglobin. Preliminary results obtained using pure proteins systems indicated that the PEG molecular weight and concentration, the pH value and the salts concentration had a pronounced effect on the partitioning behaviour of all proteins. For high ionic strengths and pH values higher than the isoelectric point (pI) of the contaminant proteins, IgG could be selectively recovered on the top phase. According to these results, a face centred composite design was performed in order to optimise the purification of IgG from the mixture of proteins. The optimal conditions for the isolation of IgG were observed for high concentrations of NaCl and low concentrations of both phase forming components. The best purification was achieved using an ATPS containing 8% (w/w) PEG 3350, 10% (w/w) phosphate pH 6 and 15% (w/w) NaCl. A recovery yield of 101+/-7%, a purity of 99+/-0% and a yield of native IgG of 97+/-4% were obtained. Back extraction studies of IgG to a new phosphate phase were performed and higher yields were obtained using 10% phosphate buffer at pH 6. The total extraction yield was 76% and the purity 100%. |
Address |
IBB Institute for Biotechnology and Bioengineering, Centre for Biological and Chemical Engineering, Instituto Superior Tecnico, Av Rovisco Pais, 1049-001 Lisbon, Portugal |
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English |
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Edition |
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ISSN |
0021-9673 |
ISBN |
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Notes |
PMID:17196214 |
Approved |
no |
Call Number |
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Serial |
1842 |
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Author |
Sarter, M. |
Title |
Animal cognition: defining the issues |
Type |
Journal Article |
Year |
2004 |
Publication |
Neuroscience and Biobehavioral Reviews |
Abbreviated Journal |
Neurosci Biobehav Rev |
Volume |
28 |
Issue |
7 |
Pages |
645-650 |
Keywords |
Animals; Behavior, Animal/*physiology; Cognition/*physiology; Humans; *Models, Animal; Psychopharmacology/methods; Reproducibility of Results |
Abstract |
The assessment of cognitive functions in rodents represents a critical experimental variable in many research fields, ranging from the basic cognitive neurosciences to psychopharmacology and neurotoxicology. The increasing use of animal behavioral tests as 'assays' for the assessment of effects on learning and memory has resulted in a considerable heterogeneity of data, particularly in the field of behavioral and psycho pharmacology. The limited predictive validity of changes in behavioral performance observed in standard animal tests of learning and memory indicates that a renewed effort to scrutinize the validity of these tests is warranted. In humans, levels of processing (effortful vs. automatic) and categories of information (procedural vs. episodic/declarative) are important variables of cognitive operations. The design of tasks that assess the recall of 'episodic' or 'declarative' information appears to represent a particular challenge for research using laboratory rodents. For example, the hypothesis that changes in inspection time for a previously encountered place or object are based on the recall of declarative/episodic information requires substantiation. In order to generalize findings on the effects of neuronal or pharmacological manipulations on learning and memory, obtained from one species and one task, to other species and other tasks, the mediating role of important sets of variables which influence learning and memory (e.g. attentional, affective) needs to be determined. Similar to the view that a neuronal manipulation (e.g. a lesion) represents a theory of the condition modeled (e.g. a degenerative disorder), an animal behavioral task represents a theory of the behavioral/cognitive process of interest. Therefore, the test of hypotheses regarding the validity of procedures used to assess cognitive functions in animals is an inherent part of the research process. |
Address |
Department of Psychology, University of Michigan, 4032 East Hall, 525 E. University Avenue, Ann Arbor, MI 48109-1109, USA. msarter@umich.edu |
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English |
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Edition |
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ISSN |
0149-7634 |
ISBN |
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Notes |
PMID:15555674 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2804 |
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Author |
Touma, C.; Palme, R.; Sachser, N. |
Title |
Analyzing corticosterone metabolites in fecal samples of mice: a noninvasive technique to monitor stress hormones |
Type |
Journal Article |
Year |
2004 |
Publication |
Hormones and Behavior |
Abbreviated Journal |
Horm Behav |
Volume |
45 |
Issue |
1 |
Pages |
10-22 |
Keywords |
Adrenal Cortex/drug effects; Adrenal Cortex Function Tests; Adrenocorticotropic Hormone/pharmacology; Analysis of Variance; Animals; Circadian Rhythm; Corticosterone/*analysis/metabolism; Dexamethasone/pharmacology; Feces/*chemistry; Female; Immunoenzyme Techniques/*methods; Male; Mice; Mice, Inbred C57BL; Models, Animal; Reproducibility of Results; Stress, Psychological/*metabolism |
Abstract |
In small animals like mice, the monitoring of endocrine functions over time is constrained seriously by the adverse effects of blood sampling. Therefore, noninvasive techniques to monitor, for example, stress hormones in these animals are highly demanded in laboratory as well as in field research. The aim of our study was to evaluate the biological relevance of a recently developed technique to monitor stress hormone metabolites in fecal samples of laboratory mice. In total, six experiments were performed using six male and six female mice each. Two adrenocorticotropic hormone (ACTH) challenge tests, two dexamethasone (Dex) suppression tests and two control experiments [investigating effects of the injection procedure itself and the diurnal variation (DV) of glucocorticoids (GCs), respectively] were conducted. The experiments clearly demonstrated that pharmacological stimulation and suppression of adrenocortical activity was reflected accurately by means of corticosterone metabolite (CM) measurements in the feces of males and females. Furthermore, the technique proved sensitive enough to detect dosage-dependent effects of the ACTH/Dex treatment and facilitated to reveal profound effects of the injection procedure itself. Even the naturally occurring DV of GCs could be monitored reliably. Thus, our results confirm that measurement of fecal CM with the recently established 5alpha-pregnane-3beta,11beta,21-triol-20-one enzyme immunoassay is a very powerful tool to monitor adrenocortical activity in laboratory mice. Since mice represent the vast majority of all rodents used for research worldwide and the number of transgenic and knockout mice utilized as animal models is still increasing, this noninvasive technique can open new perspectives in biomedical and behavioral science. |
Address |
Department of Behavioural Biology, University of Muenster, D-48149 Muenster, Germany. touma@uni-muenster.de |
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English |
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0018-506X |
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Notes |
PMID:14733887 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4084 |
Permanent link to this record |