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Bouman Jg,. (1979). A possible stallion exchange strategy in order to decrease inbreeding in the Przewalski horse. In L. E. M. de Boer, J. Bouman, & I. Bouman (Eds.), Genetics and Hereditary Diseases of the Przewalski horse (119). Rotterdam: er Foundation for the Preservation and Protection of the Przewalski Horse.
Abstract: Boer, Leobert E.M. de, and Jan & Inge Bouman, eds. Genetics and Hereditary Diseases of the Przewalski Horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse 1979. 176p, several photos and charts. Reproduced from typescript, as issued. Very good paperbound (covers shelf-soiled). ** Publishes 18 papers given at the Arnhem Study-Conference of 1978, on many aspects of breeding Przewalski horses, their behavior, etc.
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Oakenfull, E. A., Lim, H., & Ryder, O. (2000). A survey of equid mitochondrial DNA: Implications for the evolution, genetic diversity and conservation of Equus. Conservat Genet, 1(4), 341–355.
Abstract: The evolution, taxonomy and conservation of the genus Equuswere investigated by examining the mitochondrial DNA sequences of thecontrol region and 12S rRNA gene. The phylogenetic analysis of thesesequences provides further evidence that the deepest node in thephylogeny of the extant species is a divergence between twolineages; one leading to the ancestor of modern horses (E.ferus, domestic and przewalskii) and the other to thezebra and ass ancestor, with the later speciation events of the zebrasand asses occurring either as one or more rapid radiations, or withextensive secondary contact after speciation. Examination of the geneticdiversity within species suggested that two of the E. hemionussubspecies (E. h. onager and E. h. kulan) onlyrecently diverged, and perhaps, are insufficiently different to beclassified as separate subspecies. The genetic divergence betweendomestic and wild forms of E. ferus (horse) and E.africanus (African ass) was no greater than expected within anequid species. In E. burchelli (plains zebra) there was anindication of mtDNA divergence between populations increasing withdistance. The implications of these results for equid conservation arediscussed and recommendations are made for conservation action.
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Murphy, M. A., Waits, L. P., Kendall, K. C., Wasser, S. K., Higbee, J. A., & Bogden, R. (2002). An evaluation of long-term preservation methods for brown bear (Ursus arctos) faecal DNA samples. Conservat. Genet., 3(4), 435–440.
Abstract: Relatively few large-scale faecal DNA studieshave been initiated due to difficulties inamplifying low quality and quantity DNAtemplate. To improve brown bear faecal DNA PCRamplification success rates and to determinepost collection sample longevity, fivepreservation methods were evaluated: 90%ethanol, DETs buffer, silica-dried, oven-driedstored at room temperature, and oven-driedstored at -20 °C. Preservationeffectiveness was evaluated for 50 faecalsamples by PCR amplification of a mitochondrialDNA (mtDNA) locus (~146 bp) and a nuclear DNA(nDNA) locus (~200 bp) at time points of oneweek, one month, three months and six months. Preservation method and storage timesignificantly impacted mtDNA and nDNAamplification success rates. For mtDNA, allpreservation methods had >= 75% success atone week, but storage time had a significantimpact on the effectiveness of the silicapreservation method. Ethanol preserved sampleshad the highest success rates for both mtDNA(86.5%) and nDNA (84%). Nuclear DNAamplification success rates ranged from 26-88%, and storage time had a significant impacton all methods but ethanol. Preservationmethod and storage time should be importantconsiderations for researchers planningprojects utilizing faecal DNA. We recommendpreservation of faecal samples in 90% ethanolwhen feasible, although when collecting inremote field conditions or for both DNA andhormone assays a dry collection method may beadvantageous.
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Plumer, L., Talvi, T., Männil, P., & Saarma, U. (2018). Assessing the roles of wolves and dogs in livestock predation with suggestions for mitigating human-wildlife conflict and conservation of wolves. Conservat. Genet., 19(3), 665–672.
Abstract: Predation on livestock is a cause of serious and long-lasting conflict between farmers and wildlife, promoting negative public attitudes and endangering conservation of large carnivores. However, while large carnivores, especially the grey wolf (Canis lupus), are often blamed for killing sheep and other farm animals, free-ranging dogs may also act as predators. To develop appropriate measures for livestock protection, reliable methods for identifying predator species are critical. Identification of predators from visual examination of livestock wounds can be ambiguous and genetic analysis is strongly preferable for accurate species determination. To estimate the proportion of wolves and dogs implicated in sheep predation, we developed a sensitive genetic assay to distinguish between wolves and domestic dogs. A total of 183 predator saliva samples collected from killed sheep in Estonia were analysed. The assay identified the predator species in 143 cases (78%). Sheep were most often killed by wolves (81%); however, predation by dogs was substantial (15%). We compared the molecular results with field observations conducted by local environmental officials and recorded some disagreement, with the latter underestimating the role of dogs. As predator saliva samples collected from prey are often of poor quality, we suggest using mitochondrial DNA as a primary tool to maximise the number of successfully analysed samples. We also suggest adopting forensic DNA analysis more widely in livestock predation assessments as a legislative measure since misidentification that is biased against wolves can be counterproductive for conservation by enhancing conflict with society and leading to increased culling and poaching.
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Boice, R. (1981). Behavioral comparability of wild and domesticated rats. Behav Genet, 11(5), 545–553.
Abstract: The oft-repeated concern for the lack of behavioral comparability of domestic rats with wild forms of Rattus norvegicus is unfounded. Laboratory rats appear to show the potential for all wild-type behaviors, including the most dramatic social postures. Moreover, domestics are capable of assuming a feral existence without difficulty, one where they readily behave in a fashion indistinguishable from wild rats. The one behavioral difference that is clearly established concerns performance in laboratory learning paradigms. The superiority of domestics in these laboratory tasks speaks more to quieting the concerns of degeneracy theorists than to problems of using domestic Norway rats as subjects representative of their species.
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Bouman Jg,. (1979). Breeding Przewalski horse in captivity. In L. E. M. de Boer, J. Bouman, & I. Bouman (Eds.), Genetics and Herdeditary Diseases of the Przewalski horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse.
Abstract: Boer, Leobert E.M. de, and Jan & Inge Bouman, eds. Genetics and Hereditary Diseases of the Przewalski Horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse 1979. 176p, several photos and charts. Reproduced from typescript, as issued. Very good paperbound (covers shelf-soiled). ** Publishes 18 papers given at the Arnhem Study-Conference of 1978, on many aspects of breeding Przewalski horses, their behavior, etc.
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Ishida, N., Hirano, T., & Mukoyama, H. (1994). Detection of aberrant alleles in the D-loop region of equine mitochondrial DNA by single-strand conformation polymorphism (SSCP) analysis. Anim Genet, 25(4), 287.
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Bouman-Heinsdijk I,. (1979). Does inbreeding occur in free living horses? In L. E. M. de Boer, J. Bouman, & I. Bouman (Eds.), Genetics and Hereditary Diseases of the Przewalski Horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse.
Abstract: Boer, Leobert E.M. de, and Jan & Inge Bouman, eds. Genetics and Hereditary Diseases of the Przewalski Horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse 1979. 176p, several photos and charts. Reproduced from typescript, as issued. Very good paperbound (covers shelf-soiled). ** Publishes 18 papers given at the Arnhem Study-Conference of 1978, on many aspects of breeding Przewalski horses, their behavior, etc.
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Bouman Jg,. (1979). Does inbreeding ocur in free living horses? In L. E. M. de Boer, J. Bouman, & I. Bouman (Eds.), Genetics and Hereditary Diseases of the Przewalski horse (pp. 125–132). Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse.
Abstract: Boer, Leobert E.M. de, and Jan & Inge Bouman, eds. Genetics and Hereditary Diseases of the Przewalski Horse. Rotterdam: Foundation for the Preservation and Protection of the Przewalski Horse 1979. 176p, several photos and charts. Reproduced from typescript, as issued. Very good paperbound (covers shelf-soiled). ** Publishes 18 papers given at the Arnhem Study-Conference of 1978, on many aspects of breeding Przewalski horses, their behavior, etc.
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Wallner, B., Brem, G., Muller, M., & Achmann, R. (2003). Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus. Anim Genet, 34(6), 453–456.
Abstract: The phylogenetic relationship between Equus przewalskii and E. caballus is often a matter of debate. Although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtDNA sequences. Here we report sequence variation from five newly identified Y chromosome regions of the horse. Two fixed nucleotide differences on the Y chromosome clearly display Przewalski's horse and domestic horse as sister taxa. At both positions the Przewalski's horse haplotype shows the ancestral state, in common with the members of the zebra/ass lineage. We discuss the factors that may have led to the differences in mtDNA and Y-chromosomal observations.
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