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Kordal, R.J.; Parsons, S.M. |
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Title |
Liver alcohol dehydrogenase subunit equivalence studied by rapid sampling of alcohol product formed from sequentially bound [4α-3H]NADH |
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Journal Article |
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Year |
1979 |
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Archives of Biochemistry and Biophysics |
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194 |
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2 |
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439-448 |
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Horse liver alcohol dehydrogenase has been claimed to exhibit presteady-state “half-of-the-sites” reactivity with aromatic substrates under some circumstances. To clarify the role of half-of-the-sites reactivity in liver alcohol dehydrogenase the direct sampling of the alcohol product formed immediately after initiation of the reaction was studied using a rapid sampling device and [4α-3H]NADH. Liver alcohol dehydrogenase which contained a very low mole-ratio of [4α-3H]NADH bound to one subunit of the dimer was rapidly mixed with excess 4-(2'-imidazolylazo)benzaldehyde substrate and nonradioactive NADH to initiate the reaction, which was allowed to proceed for a short time before it was quenched. If strong HClO4 quench was used isolation of total free and bound azoalcohol product was possible. If NaOH quench was used isolation only of the azoalcohol product released by the enzyme was possible since most enzyme-bound azoalcohol was reversed back to azoaldehyde by the base. The pH-jump reversal reaction also was characterized spectroscopically by stopped flow technique. Nearly fullsites reactivity was observed for reaction in either direction. Furthermore (4α-3H]NADH bound firstly to one subunit in the dimer reacted essentially identically to NADH bound secondly to the other subunit. Thus, half-of-the-sites reactivity was not observed in these experiments nor did they give any indication of liver alcohol dehydrogenase active site nonequivalence induced by coenzyme binding or reaction. |
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Graham, M.; Letz, R. |
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Title |
Within-species variation in the development of ultrasonic signaling of preweanling rats |
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Journal Article |
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1979 |
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Developmental Psychobiology |
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Dev Psychobiol |
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12 |
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2 |
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129-136 |
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Animals; *Animals, Newborn; Animals, Suckling; Body Weight; Cold/adverse effects; Environment; Genotype; Individuality; Object Attachment; Rats; Stress/*physiopathology; Ultrasonics; *Vocalization, Animal |
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The development of litter and individual differences in the rate of ultrasonic signaling of neonatal rats was studied. Systematic variations among litters and individuals emerged, without differential treatment. These differences were not correlated with variations in general development as indexed by body weight. Two experiments using a cross-fostering design showed that litter differences developed independently of variations in postnatal environment. These results indicate that the variations among litters in ultrasound rate have a prenatal, possibly genetic, etiology and may represent reliable indicants of response to environmental stress. |
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0012-1630 |
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PMID:456751 |
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Equine Behaviour @ team @ |
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4172 |
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Houpt, K.A. |
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Intelligence of the horse |
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1979 |
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Equine Pract. |
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1 |
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20-26 |
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Cited By (since 1996): 5; Export Date: 24 October 2008 |
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Admin @ knut @ |
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4597 |
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Roberts, S. |
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Order and Dispute: An Introduction to Legal Anthropology |
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Miscellaneous |
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1979 |
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Equine Behaviour @ team @ |
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4931 |
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Harrington, F.H.; Mech, L.D. |
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Title |
Wolf howling and its role in territory maintenance |
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1979 |
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Behaviour |
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68 |
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Equine Behaviour @ team @ Harrington1979 |
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6455 |
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