Abstract: Some aspects of lateralization are widespread. This is clear for the association between left-eye (LE) use and readiness to respond intensely to releasing stimuli presented by others, which has been found in representatives of all major groups of tetrapods and in fishes. In the chick, this behavioural asymmetry is linked developmentally to greater ability to sustain response against distracting stimuli with right-eye (RE) use, in that both reverse with the reversal of the normal RE exposure to light. In the zebrafish, the same two asymmetries (normally) have similar associations with the LE and the RE, and both also reverse together (owing to epithalamic reversal). Here, we show that light exposure early in development is needed in zebrafish to generate both asymmetries. Dark development largely abolishes both the enhanced abilities, confirming their linkage. Resemblance to the chick is increased by the survival in the chick, after dark development, of higher ability to assess familiarity of complex stimuli when using the LE. A somewhat similar ability survives in dark-developed zebrafish. Here, LE use causes lesser reliance on a single recent experience than on longer term past experience in the assessment of novelty. Such resemblances between a fish and a bird suggest that we should look not only for resemblances between different groups of vertebrates in the most common overall pattern of lateralization, but also for possible resemblances in the nature of inter-individual variation and in the way in which it is generated during development.

Abstract: Pferdegerechte Haltungssysteme spielen bei der heutigen Nutzung des Freizeit- oder Sportpartners Pferd eine wichtige Rolle, da naturnahe Haltungsbedingungen nur in seltenen Fällen zu realisieren sind. Sämtliche Aufstallungsformen müssen als Kompromiss angesehen werden und bieten somit Vor- und Nachteile. Die vorliegende Untersuchung verfolgte den Zweck, die Stressbelastung von Pferden in verschiedenen Haltungssystemen zu erfassen und vergleichend zu beurteilen. Während der von Mai 2006 bis Juli 2007 durchgeführten Versuche wurden 24 zwei- bis dreijährige Hannoveranerstuten für jeweils 4 Wochen in insgesamt 6 verschiedenen Haltungsformen aufgestallt. Die Haltungsvarianten beinhalteten die Einzelbox (Variante 1), die Einzelbox mit einem frei zugänglichem Paddock (Variante 2), die gemeinsame Haltung von zwei Pferden in zwei Boxen und einem angeschlossenen frei zugänglichem Auslauf (Variante 3) und die Gruppenhaltung von 6 Pferden in einer Mehrraumauslaufhaltung (Variante 4 – 6). Die Haltungsvariante 4 differierte von den Varianten 5 und 6 durch seinen ungegliederten Liegebereich. In den Variante 5 und 6 wurde der Liegebereich der Gruppenhaltung durcheine über die halbe Breite reichende Trennwand in zwei Bereiche unterteilt. Die Trennwand befand sich in Haltungsvariante 5 im rechten Winkel an die Außenwand grenzend, während sie in Variante 6 an der der Stallgasse zugewandten Seite des Liegebereichs aufgestellt wurde. Um eine Vergleichbarkeit mit einem Reitpferd zu schaffen, wurden alle Pferde in allen Haltungssystemen täglich für circa eine Stunde in einer Freilaufanlage bewegt. Die Stressbelastung wurde mittels der Messung der Herzfrequenzvariabilität (Parameter pNN50 und SD1) und der Bestimmung fäkaler Cortisolmetaboliten erfasst. Zusätzlich wurden Videoauswertungen hinsichtlich der Beobachtung von Verhaltensauffälligkeiten in den Haltungsvarianten 1 und 2 durchgeführt. Die durchschnittlich niedrigste Stressbelastung erfuhren die Pferde durch die Haltungsvarianten 4 (pNN50: 46,32 %, SD1:158,58 ms, Cortisolmetaboliten: 21,01nmol/kg Kot) und 6 (pNN50: 47,1 %, SD1: 144,62 ms, Cortisolmetaboliten: 21,01 nmol/kg Kot). DieAuswertung der pNN50- und Cortisolmetabolitenwerte ergab die größte Stressbelastung der Pferde in den Varianten 1 (pNN50: 42,81 %, SD1: 134,52 ms, Cortisolmetaboliten: 28,56 nmol/kg Kot) und 3 (pNN50: 42,41 %, SD1: 135,36, Cortisolmetaboliten: 28,60 nmol/kg Kot). Die Auswertung der SD1-Werte zeigte zusätzlich noch eine hohe Stressbelastung der Pferde in der Variante 5 (pNN50: 44,83 %, SD1: 119,24 ms, Cortisolmetaboliten: 27,18 nmol/kg Kot). Die Haltungsvariante 2 (pNN50: 45,77 %, SD1: 144,25 ms, Cortisolmetaboliten: 27,59 nmol/kg Kot) beziehungsweise die Varianten 2 und 5 (bei der Betrachtung der pNN50- und der Cortisolmetabolitenwerte) verursachten im Durchschnitt eine mittlere Stressbelastung. Teilweise waren die Unterschiede jedoch zu gering um die Signifikanzgrenze zu überschreiten. Die Betrachtung der einzelnen Pferde und der Pferdegruppen ergab große interindividuelle Unterschiede und deutliche gruppenspezifische Gemeinsamkeiten. Die Position eines Tieres in der Rangordnung hatte in diesen Untersuchungen bei der Betrachtung der Mittelwerte keinen Einfluss auf die Stressbelastung in Abhängigkeit derHaltungsvariante. Vielmehr zeigten sich auch hier deutliche individuelle Unterschiede in der Stressanfälligkeit und den Vorlieben der einzelnen Pferde bezüglich der verschiedenen Haltungssysteme. Die Analyse der Videoaufzeichnungen ergab, dass mehrere Pferde Kreisbewegungen in der Einzelbox ohne Auslauf (Variante 1) zeigten, während in der Variante 2 (Paddockbox) kein Pferd durch Kreisbewegungen auffiel. Andere Verhaltensauffälligkeiten oder gar Verhaltensstörungen wurden nicht beobachtet. Bei der Auswertung der Aufenthaltshäufigkeit und der Aufenthaltesdauer in den 4 Boxenquadranten zeigten sich einige Pferde sehr aktiv, was ein Hinweis auf eventuelle Unruhe oder Nervosität sein kann, während andere Pferde im Vergleich zum Gruppendurchschnitt sehr ruhig wirkten, da sie sich in der Box kaum bewegten und über lange Zeitabschnitte in einem Quadranten standen. Obwohl die Gruppenhaltung für die Mehrzahl der Pferde eine geringere Stressbelastung bedeutete und auch hinsichtlich des Bewegungs- und Sozialverhaltens am artgerechtesteneinzustufen ist, sollte für jedes Pferd individuell unter Berücksichtigung seines Alters, seiner Rasse, seines Nutzungsgrades, aber vor allem seines Charakters und seiner Erfahrungen im Sozialverhalten ein passendes großzügig bemessenes Haltungssystem ermittelt werden, um die durch das Haltungssystem ausgeübte Stressbelastung zu minimieren und das Wohlbefinden der Pferde zu steigern.
[Horse-appropriate husbandry plays an important role in the current recreational use of sport horses, since close-to-nature husbandry conditions can only be realized in rare cases. All forms of stables must be seen as a compromise and thus offer both advantages and disadvantages. The current studies were aimed at documenting and comparatively judging the stress on horse in various husbandry systems. In the studies conducted from May 2006 to July 2007, 24 two- to three-year old Hanover mares were each kept for four weeks in a total of six different types of husbandry. The husbandry forms included the single box (Variation 1), single box with a freely accessible paddock (Variation 2), the common keeping of two horses in two boxes and an attached freely accessible yards (Variation 3) and group husbandry of six horses in a multi-room yards husbandry facility (Variations 4-6). Husbandry Variation 4 differs from Variations 5 and 6 through its non-divided lying area. In the Variations 5 and 6, the lying area of the group is divided through a dividing wall extending over half the width of the area. The dividing wall in husbandry variation 5 was at a right angle to the external wall, while in Variation 6 it was placed on the stable passage side of the lying areas. In order to create comparability with riding horses, all horses in the husbandry systems were moved for about one hour on an area in a free yard. The stress level was measured on the basis of heart frequency variability (Parameter pNN50 and SD1) and the determination of faecal Cortisol metabolites. In addition video evaluations were made of conspicuous behaviour in the Variations 1 and 2. The average lowest stress was experienced by the horses in Variation 4 (pNN50: 46.32 %, SD1: 158.58 ms, Cortisol metabolites: 21.01 nmol/kg faeces) and 6 (pNN50: 47.1 %, SD1: 144.62 ms, Cortisol metabolites: 21.01 nmol/kg faeces). The evaluation of the pNN50 and the Cortisol metabolite values resulted in the greatest stress for the horses in Variation 1(pNN50: 42.81 %, SD1: 134,52 ms, Cortisol metabolites: 28,56 nmol/kg faeces) and 3 (pNN50: 42.41 %, SD1: 135.36 ms, Cortisol metabolites: 28.60 nmol/kg faeces). The evaluation of the SD1 values also showed additional stress for the horses in Variation 5 (pNN50: 44.83 %, SD1: 119.24 ms, Cortisol metabolites: 27.18 nmol/kg faeces). Variation 2 (pNN50: 45.77 %, SD1: 144.25 ms, Cortisol metabolites: 27.59 nmol/kg faeces) or rather the Variations 2 and 5 (in observing the pNN50- and Cortisol metabolite values) caused on average a medium stress level. Sometimes the differences were too low to exceed the level of significance. The observation of individual horses and groups of horses resulted in great inter-individual differences and clear group-specific points in common. The position of the horse in the animal hierarchy had no influence on the stress on the animals in this study. Much more, the clear individual differences in susceptibility to stress and a preference of the individual horses for different husbandry systems. The analyses of the videos show that more horses showed circular movements in the single stable without a yard (Variation 1), while in Variation 2 no horse was conspicuous with circular movements. Other conspicuous or disturbed behaviour was not observed. In the evaluation of the frequency and duration of presence in the four quadrant boxes, some horses were very active, a sign of possible excitement or nervousness, while other horses appeared very quiet in comparison to the group average, since they hardly moved in the box and stood in the quadrants for longer periods of time. Although the group husbandry presented less stress for the majority of the horses, also in regard to the most animal appropriate movement and social behaviour, an individual evaluation of each horse should be made considering his age, race, and type of use, but above all considering his character and experiences in social behaviour in the appropriate husbandry system in order to minimize the stress caused by the husbandry system and to increase the horse’s well-being.]

Abstract: Despite their ability to interbreed and produce fertile offspring, there is continued disagreement about the genetic relationship of the domestic horse (Equus caballus) to its endangered wild relative, Przewalski's horse (Equus przewalskii). Analyses have differed as to whether or not Przewalski's horse is placed phylogenetically as a separate sister group to domestic horses. Because Przewalski's horse and domestic horse are so closely related, genetic data can also be used to infer domestication-specific differences between the two. To investigate the genetic relationship of Przewalski's horse to the domestic horse and to address whether evolution of the domestic horse is driven by males or females, five homologous introns (a total of [~]3 kb) were sequenced on the X and Y chromosomes in two Przewalski's horses and three breeds of domestic horses: Arabian horse, Mongolian domestic horse, and Dartmoor pony. Five autosomal introns (a total of [~]6 kb) were sequenced for these horses as well. The sequences of sex chromosomal and autosomal introns were used to determine nucleotide diversity and the forces driving evolution in these species. As a result, X chromosomal and autosomal data do not place Przewalski's horses in a separate clade within phylogenetic trees for horses, suggesting a close relationship between domestic and Przewalski's horses. It was also found that there was a lack of nucleotide diversity on the Y chromosome and higher nucleotide diversity than expected on the X chromosome in domestic horses as compared with the Y chromosome and autosomes. This supports the hypothesis that very few male horses along with numerous female horses founded the various domestic horse breeds. Patterns of nucleotide diversity among different types of chromosomes were distinct for Przewalski's in contrast to domestic horses, supporting unique evolutionary histories of the two species.

Abstract: We investigated whether cattle exhibit preferences to monitor challenging and novel stimuli. Experiments were conducted on dairy and beef cattle herds and revealed significant left eye preferences in the cattle for viewing an experimenter walking to repeatedly split the herd through its centre. Visual lateralisation was demonstrated in the preference to use the left monocular field to monitor the experimenter, alone or equipped with a range of novel stimuli. This finding is consistent with left eye preferences found in various species of mammals, birds, and amphibians responding to predators and novel stimuli. A cohort of the familiarised cattle herds was then subjected to additional herd-splitting tests with the same stimuli and demonstrated a reversal of viewing preferences, preferring to monitor the experimenter and stimuli within the right and not left monocular field. This directional shift in viewing preferences is consistent with experience-dependent learning found in lateralised visual processing in other, non-mammalian, species, and to our knowledge is the first of such studies to suggest that such lateralised learning processes also exist in mammals. Together the data support a number of key hypotheses concerning the evolution and conservation of lateralised brain function in vertebrates, and also provide important considerations for livestock handling.