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| Author | Rodier, F. | ||||
| Title | [Spectral properties of porcine plasminogen: study of the acidic transition (author's transl)] | Type | Journal Article | ||
| Year | 1976 | Publication | European journal of biochemistry / FEBS | Abbreviated Journal | Eur J Biochem |
| Volume | 63 | Issue | 2 | Pages | 553-562 |
| Keywords | Animals; Binding Sites; Guanidines; Hydrogen-Ion Concentration; *Plasminogen; Protein Binding; Protein Conformation; Spectrometry, Fluorescence; Spectrophotometry; Spectrophotometry, Ultraviolet; Swine; Temperature | ||||
| Abstract | The acidic transition of porcine plasminogen, prepared by affinity chromatography, was studied by non-destructive methods. These methods are based on the analysis of the behaviour of the tryptophyls under various conditions. The perturbation of the absorption and emission spectra by pH or temperature and the dynamic quenching of the intrinsic fluorescence are used to obtain information on structural changes which affect the environment of these residues. It is shown that by decreasing pH the fluorescence emission spectra are shifted toward the long wavelengths, with a broadening of the fluorescence band. The same effect can be obtained at constant pH by heating the protein solution. In order to analyze these phenomena, it is assumed that the fluorescence intensities at 355 nm and 328 nm reflect the proportion of the tryptophans which are exposed to the solvent, and buried, respectively. The plot of the ratio of the fluorescence intensities at these wavelengths versus pH or temperature leads to a titration curve showing an unmasking of tryptophans. The proportion of exposed tryptophans is measured by the dynamic fluorescence quenching technique and the data analyzed according to Lehrer. The plot of the fraction of exposed tryptophyls versus pH also shows the unmasking of these chromophores. Thermal perturbation of a solution of plaminogen at neutral pH induces a difference absorption spectrum whose amplitudes at the maxima are proportional to the number of exposed aromatic residues. The comparison with a solution of fully denatured plasminogen in 6 M guanidium chloride, where all the tryptophyls are exposed, shows that the percentage of exposure is equal to 59%. This number is significantly higher than the percentage found by the fluorescence quenching technique (20%), indicating that some tryptophyls are located in crevices, exposed to the solvent but not to the iodide. At acidic pH the absorption difference spectra induced by thermal perturbation are not classical, since they show an inversion and a new band between 300 nm and 305 nm. This band is mentioned in the literature as a minor band of tryptophan which appears when this chromophore is located in an asymmetric environment. On plotting the maximum amplitude of these spectra obtained at acidic pH versus temperature, we obtain a curve indicating that two types of antagonistic interactions are involved in the perturbation of the chromophores spectra. The spectrophotometric titration of plasminogen gives classical absorption difference spectra. By plotting the maximum amplitude at 292 nm versus pH, we obtain a titration curve with an apparent pK of 2.9 units. This pK is acidic which respect to the pK value of a normal carboxyl. This low value can be due to a positively charged group in the neighbourhood of a carboxyl, which interacts with one or more chromophores. When the carboxyl becomes protonated, this positively charged group is free and available to perturb the environment of some chromophores... | ||||
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| Language | French | Summary Language | Original Title  |
Proprietes spectrales du plasminogene porcin. Etude de la transition acide | |
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| ISSN | 0014-2956 | ISBN | Medium | ||
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| Notes | PMID:4326 | Approved | no | ||
| Call Number | Admin @ knut @ | Serial | 22 | ||
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| Author | Arnold, W.; Ruf, T.; Kuntz, R. | ||||
| Title | Seasonal adjustment of energy budget in a large wild mammal, the Przewalski horse (Equus ferus przewalskii) II. Energy expenditure | Type | Journal Article | ||
| Year | 2006 | Publication | The Journal of experimental biology | Abbreviated Journal | J Exp Biol |
| Volume | 209 | Issue | Pt 22 | Pages | 4566-4573 |
| Keywords | Animals; Animals, Wild/*physiology; Body Temperature; Body Temperature Regulation; Eating; *Energy Metabolism; Female; Heart Rate; Horses/*physiology; Male; Motor Activity; Pregnancy; Reproduction; *Seasons | ||||
| Abstract | Many large mammals show pronounced seasonal fluctuations of metabolic rate (MR). It has been argued, based on studies in ruminants, that this variation merely results from different levels of locomotor activity (LA), and heat increment of feeding (HI). However, a recent study in red deer (Cervus elaphus) identified a previously unknown mechanism in ungulates--nocturnal hypometabolism--that contributed significantly to reduced energy expenditure, mainly during late winter. The relative contribution of these different mechanisms to seasonal adjustments of MR is still unknown, however. Therefore, in the study presented here we quantified for the first time the independent contribution of thermoregulation, LA and HI to heart rate (f(H)) as a measure of MR in a free-roaming large ungulate, the Przewalski horse or Takhi (Equus ferus przewalskii Poljakow). f(H) varied periodically throughout the year with a twofold increase from a mean of 44 beats min(-1) during December and January to a spring peak of 89 beats min(-1) at the beginning of May. LA increased from 23% per day during December and January to a mean level of 53% per day during May, and declined again thereafter. Daily mean subcutaneous body temperature (T(s)) declined continuously during winter and reached a nadir at the beginning of April (annual range was 5.8 degrees C), well after the annual low of air temperature and LA. Lower T(s) during winter contributed considerably to the reduction in f(H). In addition to thermoregulation, f(H) was affected by reproduction, LA, HI and unexplained seasonal variation, presumably reflecting to some degree changes in organ mass. The observed phase relations of seasonal changes indicate that energy expenditure was not a consequence of energy uptake but is under endogenous control, preparing the organism well in advance of seasonal energetic demands. | ||||
| Address | Research Institute of Wildlife Ecology, University of Veterinary Medicine, Vienna, Savoyenstrasse 1, 1160 Vienna, Austria. walter.arnold@vu-wien.ac.at | ||||
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| ISSN | 0022-0949 | ISBN | Medium | ||
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| Notes | PMID:17079726 | Approved | no | ||
| Call Number | Serial | 1782 | |||
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| Author | Hrdy, S.B. | ||||
| Title | Male-male competition and infanticide among the langurs (Presbytis entellus) of Abu, Rajasthan | Type | Journal Article | ||
| Year | 1974 | Publication | Folia Primatologica; International Journal of Primatology | Abbreviated Journal | Folia Primatol (Basel) |
| Volume | 22 | Issue | 1 | Pages | 19-58 |
| Keywords | Aggression; Animals; Animals, Newborn; Coitus; *Competitive Behavior; Estrus; Feeding Behavior; Female; *Haplorhini; Homing Behavior; Humans; India; Infanticide; Leadership; Male; Maternal Behavior; Population Density; Pregnancy; Rain; Seasons; Sex Factors; Sexual Behavior, Animal; Social Behavior; Temperature; Vocalization, Animal | ||||
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| ISSN | 0015-5713 | ISBN | Medium | ||
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| Notes | PMID:4215710 | Approved | no | ||
| Call Number | Serial | 2051 | |||
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| Author | Duncan, I.J.H.; Widowski, T.M.; Malleau, A.E.; Lindberg, A.C.; Petherick, J.C. | ||||
| Title | External factors and causation of dustbathing in domestic hens | Type | Journal Article | ||
| Year | 1998 | Publication | Behavioural Processes | Abbreviated Journal | Behav. Process. |
| Volume | 43 | Issue | 2 | Pages | 219-228 |
| Keywords | Dustbathing; Illumination; Laying hens; Radiant heat; Social facilitation; Temperature | ||||
| Abstract | Dustbathing is known to be motivated by complex interactions between internal factors which build up over time and external factors, such as the sight of a dusty substrate. In this study, the effects of other external factors were investigated. Environmental temperature was shown to be important; frequencies of dustbathing were greater when hens were held at 22 than at 10[degree sign]C (P<0.01). In a second experiment, a radiant heat source or a radiant heat+light source, balanced to give the same radiant heat, resulted in more dustbathing behaviour during a 1-h stimulus period than during the same period with no stimulus (P<0.05). Components of dustbathing were increased more by the heat+light stimulus than by the heat stimulus alone (P<0.03). In a third experiment, the amount of dustbathing performed by individual hens in cages with dustbaths was increased by the presence of a group of hens dustbathing in an adjoining pen with a dustbath compared with the amount occurring when the hens were absent from the pen. | ||||
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| Call Number | Equine Behaviour @ team @ | Serial | 3607 | ||
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| Author | Balakrishnan, G.; Hu, Y.; Spiro, T.G. | ||||
| Title | Temperature-jump apparatus with Raman detection based on a solid-state tunable (1.80-2.05 microm) kHz optical parametric oscillator laser | Type | Journal Article | ||
| Year | 2006 | Publication | Applied Spectroscopy | Abbreviated Journal | Appl Spectrosc |
| Volume | 60 | Issue | 4 | Pages | 347-351 |
| Keywords | Animals; Cytochromes c/analysis; Horses; Lasers; Myoglobin/metabolism; Spectrum Analysis, Raman/*instrumentation/*methods; *Temperature | ||||
| Abstract | The operating characteristics of a pulsed (10 ns) tunable near-infrared (NIR) laser source are described for temperature-jump (T-jump) applications. A Q-switched Nd:YLF laser (approximately 10 ns pulses) with a 1 kHz repetition rate is used to pump a potassium titanyl arsenate (KTA) crystal-based optical parametric oscillator (OPO), producing approximately 1 mJ NIR pulses that are tunable (1.80-2.05 microm) across the 1.9 microm vibrational overtone band of water. This T-jump source has been coupled to a deep ultraviolet (UV) probe laser for Raman studies of protein dynamics. T-jumps of up to 30 degrees C, as measured via the O-H stretching Raman band of water, are readily achieved. Application to cytochrome c unfolding is demonstrated. | ||||
| Address | Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA | ||||
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| Language | English | Summary Language | Original Title |
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| ISSN | 0003-7028 | ISBN | Medium | ||
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| Notes | PMID:16613628 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 3764 | ||
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| Author | Hagen, S.J.; Eaton, W.A. | ||||
| Title | Two-state expansion and collapse of a polypeptide | Type | Journal Article | ||
| Year | 2000 | Publication | Journal of Molecular Biology | Abbreviated Journal | J Mol Biol |
| Volume | 301 | Issue | 4 | Pages | 1019-1027 |
| Keywords | Animals; Computer Simulation; Cytochrome c Group/*chemistry/*metabolism; Horses; Kinetics; Lasers; Models, Chemical; Peptides/*chemistry/*metabolism; Protein Conformation; Protein Denaturation; *Protein Folding; Spectrometry, Fluorescence; Temperature; Thermodynamics | ||||
| Abstract | The initial phase of folding for many proteins is presumed to be the collapse of the polypeptide chain from expanded to compact, but still denatured, conformations. Theory and simulations suggest that this collapse may be a two-state transition, characterized by barrier-crossing kinetics, while the collapse of homopolymers is continuous and multi-phasic. We have used a laser temperature-jump with fluorescence spectroscopy to measure the complete time-course of the collapse of denatured cytochrome c with nanosecond time resolution. We find the process to be exponential in time and thermally activated, with an apparent activation energy approximately 9 k(B)T (after correction for solvent viscosity). These results indicate that polypeptide collapse is kinetically a two-state transition. Because of the observed free energy barrier, the time scale of polypeptide collapse is dramatically slower than is predicted by Langevin models for homopolymer collapse. | ||||
| Address | Laboratory of Chemical Physics, NIDDK, National Institutes of Health, Building 5, Bethesda, MD, 20892-0520, USA | ||||
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| ISSN | 0022-2836 | ISBN | Medium | ||
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| Notes | PMID:10966803 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 3790 | ||
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| Author | Kihara, H.; Nakatani, H.; Hiromi, K.; Hon-Nami, K. | ||||
| Title | Kinetic studies on redox reactions of hemoproteins. I. Reduction of thermoresistant cytochrome c-552 and horse heart cytochrome c by ferrocyanide | Type | Journal Article | ||
| Year | 1977 | Publication | Biochimica et Biophysica Acta | Abbreviated Journal | Biochim Biophys Acta |
| Volume | 460 | Issue | 3 | Pages | 480-489 |
| Keywords | Animals; Bacteria; *Cytochrome c Group; *Ferrocyanides; Horses; Kinetics; Mathematics; Oxidation-Reduction; Spectrophotometry; Spectrophotometry, Ultraviolet; Temperature; Thermodynamics | ||||
| Abstract | The oxidation-reduction reaction of horse heart cytochrome c and cytochrome c (552, Thermus thermophilus), which is highly thermoresistant, was studied by temperature-jump method. Ferrohexacyanide was used as reductant. (Formula: see text.) Thermodynamic and activation parameters of the reaction obtained for both cytochromes were compared with each other. The results of this showed that (1) the redox potential of cytochrome c-552, + 0.19 V, is markedly less than that of horse heart cytochrome c. (2) deltaHox of cytochrome c-552 is considerably lower than that of horse heart cytochrome c. (3) deltaSox and deltaSred of cytochrome c-552 are more negative than those of horse heart cytochrome c. (4) kred of cytochrome c-552 is much lower than that of horse heart cytochrome c at room temperature. | ||||
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| ISSN | 0006-3002 | ISBN | Medium | ||
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| Notes | PMID:195599 | Approved | no | ||
| Call Number | Equine Behaviour @ team @ | Serial | 3815 | ||
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| Author | Fazio, E.; Medica, P.; Cravana, C.; Giacoppo, E.; Ferlazzo, A. | ||||
| Title | Effect of Short-Distance Road Transport on Thyroid Function, Rectal Temperature, Body Weight and Heart Rate of Stallions | Type | Conference Article | ||
| Year | 2008 | Publication | IESM 2008 | Abbreviated Journal | |
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| Keywords | horses, iodothyronines, rectal temperature, body weight, heart rate, transport | ||||
| Abstract | Aim of study was to investigate the effects of transport stress on thyroid response, body weight, rectal temperature and heart rate changes in one hundred twenty-six healthy stallions in basal conditions, before and after short road transport. One hundred twenty-six Thoroughbreds and crossbreds stallions with previous travelling experience, aged 4 to 15 yr, were transported by road in a commercial trailer for a period of 3 h (distance <300 km). Blood samples and physiological parameters were collected at 0800 (basal I) and at 1100 (basal II), in each horse“s box, one week before the loading and transport in basal conditions, and one week later, at 0800 immediately before loading (pre-transport), and after 3 h period of transport and unloading, on their arrival at the breeding stations (post-transport), in each new horse”s box, within 30 min. Increases in circulating T3, T4 and fT4 levels (P < 0.01), but not for fT3 levels, were observed after transport, as compared to before loading values, irrespective of different breed. Lower T4 and fT4 levels were observed in basal II (P < 0.01) than basal I and before loading values (pre-transport). After transport Thoroughbreds showed higher fT3 (P < 0.05) and fT4 (P < 0.01) levels than crossbred stallions. No significant differences for T3 and T4 changes were observed. A significant increase in rectal temperature (P < 0.01) and heart rate (P < 0.05) was observed after transport, as compared to before loading values (pre-transport). No differences between basal I, basal II and before loading values (pre-transport) for physiological parameters were observed. The highest T3, T4 and fT4 levels recorded after short transport seem to suggest a preferential release from the thyroid gland. The results indicate that short road transport stress contributes significantly to thyroid hormone changes, according to different breed, and to the increase in rectal temperature and heart rate. No differences related to different age were observed. |
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| Address | Department of Morphology, Biochemistry, Physiology and Animal Production – Unit of Veterinary Physiology, Faculty of Veterinary Medicine, University of Messina, Polo Universitario Annunziata, 98168 Messina, Italy | ||||
| Corporate Author | Cravana, C. | Thesis | |||
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| Area | Expedition | Conference | IESM 2008 | ||
| Notes | Poster IESM 2008 | Approved | yes | ||
| Call Number | Equine Behaviour @ team @ | Serial | 4494 | ||
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| Author | Brinkmann, L.; Gerken, M.; Riek, A. | ||||
| Title | Energetic adaptations of Shetland pony mares | Type | Conference Article | ||
| Year | 2015 | Publication | Proceedings of the 3. International Equine Science Meeting | Abbreviated Journal | Proc. 3. Int. Equine. Sci. Mtg |
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| Keywords | Body temperature, Energy expenditure, Food restriction, Hypometabolism, Locomotor activity, Shetland pony | ||||
| Abstract | Recent results suggest that wild Northern herbivores exhibit signs of a hypometabolism during times of low ambient temperature and food shortage in order to reduce their energetic needs. However, there are speculations that domestic animals lost the ability to reduce energy expenditure. To examine energetic and behavioural responses 10 Shetland pony mares were exposed to different environmental conditions (summer and winter). During winter ponies were allocated into two groups receiving two different food quantities (60% and 100% of maintenance energy requirement). We measured the field metabolic rate, water turn over, body temperature, locomotor activity, lying time, resting heart rate, body mass and body condition score. In summer, the field metabolic rate of all ponies (FMR; 63.4±15.0 MJ/day) was considerably higher compared with food restricted and control animals in winter (24.6±7.8 and 15.0±1.1 MJ/day, respectively). Furthermore, during summer, locomotor activity, resting heart rate and total water turnover were significantly elevated (P<0.001) compared with winter. Animals receiving a reduced amount of food (N=5) reduced their FMR by 26% compared with control animals (N=5) to compensate for the decreased energy supply. Furthermore, resting heart rate, body mass and body condition score were lower(29.2±2.7 beats/min, 140±22 kg and 3.0±1.0 points, respectively) than in control animals (36.8±41 beats/min, 165±31 kg, 4.4±0.7 points; P<0.05). While no difference could be found in the observed behaviour, nocturnal hypothermia was elevated in restrictively fed animals. Our results indicate that ponies adapt to different climatic conditions by changing their metabolic rate, behaviour and some physiological parameters. When exposed to energy shortage, ponies, like wild herbivores, exhibited hypometabolism and nocturnal hypothermia. Keywords: Body temperature, Energy expenditure, Food restriction, Hypometabolism, Locomotor activity, Shetland pony |
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| Corporate Author | Brinkmann, L. | Thesis | |||
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| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 5893 | ||
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| Author | Oliveira-Santos, L.G.R.; Machado-Filho, L.C.P.; Tortato, M.A.; Brusius, L. | ||||
| Title | Influence of extrinsic variables on activity and habitat selection of lowland tapirs (Tapirus terrestris) in the coastal sand plain shrub, southern Brazil | Type | Journal Article | ||
| Year | 2010 | Publication | Mammalian Biology – Zeitschrift für Säugetierkunde | Abbreviated Journal | |
| Volume | 75 | Issue | 3 | Pages | 219-226 |
| Keywords | Behaviour; Circadian rhythmic; Moonlight; Rainfall; Temperature | ||||
| Abstract | The objectives of this research were to: 1. evaluate the circadian activity patterns of lowland tapirs (Tapirus terrestris) throughout the seasons and 2. study the influence of moonlight, temperature and rainfall on the activity patterns and habitat selection of this species, in the coastal sand shrub in southern Brazil. From June 2005 to June 2006, eight tapirs were monitored in a large enclosure containing open and vegetation-covered areas, using four camera traps. Differences in activity patterns within seasons were found. Tapir predominately presented nocturnal-crepuscular activity; however, they differed in the winter, with cathemeral activity patterns. Covered areas were mostly used during periods of extreme temperatures, with less diurnal and more nocturnal activities within these areas, on hotter days. Activity in open areas mainly occurred during periods of intermediate temperatures, both during the day and in the night. Moonlight intensity did not influence nocturnal activities. On days of precipitation of 34 mm or more, there was no record of open-area activities, despite constant activity in covered-area. | ||||
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| ISSN | 1616-5047 | ISBN | Medium | ||
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| Notes | Approved | no | |||
| Call Number | Equine Behaviour @ team @ | Serial | 6140 | ||
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