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Author |
Spengler M.I.; Rasia M. |
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Title |
Influence of Plasma Proteins on Erythrocyte Aggregation in Three Mammalian Species |
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Journal Article |
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2001 |
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Veterinary Research Communications |
Abbreviated Journal |
Vet.Res.Comm |
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25 |
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7 |
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591-599 |
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albumin – bovine – equine – erythrocyte aggregation – dextran – haemorheology – human – plasma protein – polyvinylpyrrolidone |
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The aggregation capacity of human erythrocytes lies between that of the non-aggregating bovine erythrocytes and the remarkably aggregating equine ones. As the ability to aggregate is attributed to cell factors and the composition of the plasma proteins, the role that plasma proteins play in the aggregation process in these three species was studied. Washed erythrocytes were suspended in phosphate-buffered saline (PBS; pH 7.4, 300 mOsm/L) plus polyvinylpyrrolidone (PVP) in a suitable concentration to obtain an average intensity of aggregation (control media). The superimposed effect of replacing 80% of the medium by either autologous plasma, serum or albumin solution was studied. The plasma proteins appeared to enhance aggregation by human and equine erythrocytes, but impaired this process in bovine erythrocytes. Some evidence was obtained supporting the existence of serum factors capable of reducing aggregation of erythrocytes in cattle and it was concluded that the non-aggregating behaviour of bovine erythrocytes may be due to the cells interacting particularly with the macromolecules in the serum. |
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2013 |
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Fazio, F.; Assenza, A.; Piccione, G.; Caola, G. |
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Title |
Periodic Monitoring of Some Physiological Parameters during Training in the Athletic Horse |
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Journal Article |
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Year |
2003 |
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Veterinary Research Communications |
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27 |
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595-598 |
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Equine Behaviour @ team @ |
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4384 |
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Author |
Fazio, E.; Ferlazzo, A. |
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Title |
Evaluation of Stress During Transport |
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Year |
2003 |
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Veterinary Research Communications |
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27 |
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519-524 |
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hormones – physiological variables – stress – transport procedures |
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Domestic animals are transported for a variety of reasons including breeding, biomedical purposes, slaughter and, in the case of sporting horses, for competitions, pleasure activities or ceremonial proceedings. Studies to determine the amount of stress on farm animals during transport often have highly variable results and are difficult to interpret. The reaction of animals to stressors depends on the duration and intensity of the stressors, the animal's previous experience, its physiological status and the immediate environmental restraints. Behavioural, haematological, haematochemical, physiological and neuro-hormonal (ß-endorphin, ACTH, cortisol, iodothyronines) variables are discussed on the basis of handling, loading and transport procedures of animals. |
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Equine Behaviour @ team @ |
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4391 |
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Author |
Flauger, B.; Krueger, K.; Gerhards, H.; Möstl, E. |
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Title |
Simplified method to measure glucocorticoid metabolites in faeces of horses |
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Journal Article |
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2010 |
Publication |
Veterinary Research Communications |
Abbreviated Journal |
Vet Res Comm |
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34 |
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2 |
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185-195 |
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ACTH challenge; enzyme immunoassay; stress behaviour; cortisol |
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Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited racticability due to its complex extraction procedure. Therefore, we tested the applicability of
other enzyme immunoassays for glucocorticoid metabolites. The present study clearly proved that an enzyme immunoassay (EIA) for 11-oxoetiocholanolone using 11-oxoetiocholanolone-17-CMO: BSA (3α,11-oxo-A EIA) as antigen showed high amounts of immunoreactive substances. Therefore it was possible to use just a small amount of the supernatant of a methanolic suspension of faeces. The results
correlated well with the already described method for measuring GCMs in horse faeces, i.e. analysing the samples with an EIA after a two step clean up procedure of the samples (Merl et al. 2000). In addition, the 3α,11-oxo-A EIA has the advantage of providing a bigger difference between baseline values and peak values after ACTH stimulation. The new assay increased the accuracy of the test,
lowered the expenses per sample, and storing samples at room temperature after collection was less critical than with other assays investigated in our study. This is a big advantage both in the field of wildlife management of equids and in the field of equestrian sports and it shows the importance of choosing an assay which is in good accordance with the metabolites excreted in a given species. |
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Equine Behaviour @ team @ |
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5073 |
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Author |
Herron, M.A.; Martin, J.E.; Joyce, J.R. |
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Title |
Quantitative study of the decussating optic axons in the pony, cow, sheep, and pig. |
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Journal Article |
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Year |
1978 |
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American Journal of Veterinary Research |
Abbreviated Journal |
Am. J. Vet .Res. |
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39 |
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7 |
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1137-9 |
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Equine Behaviour @ team @ |
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5586 |
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Author |
Rehbinder, C.; Hau, J. |
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Title |
Quantification of cortisol, cortisol immunoreactive metabolites, and immunoglobulin A in serum, saliva, urine, and feces for noninvasive assessment of stress in reindeer |
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Journal Article |
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2005 |
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Canadian Journal of Veterinary Research |
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Can J Vet Res |
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70 |
Issue |
2 |
Pages |
151-154 |
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This study was designed to develop reliable methods for quantification of cortisol and cortisol immunoreactive metabolites (C-CIM) and immunoglobulin A (IgA) in reindeer serum, saliva, urine, and feces as tools for the objective noninvasive assessment of well-being and immunocompetence in reindeer. Although C-CIM was readily quantifiable by radioimmunoassay in serum, urine, and feces, the levels in saliva samples were low, rendering quantification unreliable. Whereas IgA concentrations were high in feces samples, they were much lower, albeit quantifiable, in serum and urine; the levels in saliva samples were too low for quantification with the use of an enzyme-linked immunosorbent assay that we developed. Further studies are in progress to validate the usefulness of fecal levels of C-CIM and IgA in the assessment of welfare in reindeer. |
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Canadian Veterinary Medical Association |
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0830-9000 |
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Equine Behaviour @ team @ |
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5852 |
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Janczarek, I.; Stachurska, A.; Kedzierski, W.; Wisniewska, A.; Ryzak, M.; Koziol, A. |
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Title |
The intensity of physiological and behavioral responses of horses to predator vocalizations |
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Journal Article |
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Year |
2020 |
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BMC Veterinary Research |
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16 |
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1 |
Pages |
431 |
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Predatory attacks on horses can become a problem in some parts of the world, particularly when considering the recovering gray wolf populations. The issue studied was whether horses transformed by humans and placed in stable-pasture environments had retained their natural abilities to respond to predation risk. The objective of the study was to determine the changes in cardiac activity, cortisol concentrations, and behavior of horses in response to the vocalizations of two predators: the gray wolf (Canis lupus), which the horses of the breed studied had coevolved with but not been exposed to recently, and Arabian leopard (Panthera pardus nimr), from which the horses had been mostly isolated. In addition, we hypothesized that a higher proportion of Thoroughbred (TB) horse ancestry in the pedigree would result in higher emotional excitability in response to predator vocalizations. Nineteen horses were divided into groups of 75%, 50% and 25% TB ancestry. The auditory test conducted in a paddock comprised a 10-min prestimulus period, a 5-min stimulus period when one of the predators was heard, and a 10-min poststimulus period without any experimental stimuli. |
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1746-6148 |
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Equine Behaviour @ team @ Janczarek2020 |
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6624 |
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Author |
Knill, L.M.; Eagleton, R.D.; Harver, E. |
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Title |
Physical optics of the equine eye |
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Journal Article |
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1977 |
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American Journal of Veterinary Research |
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Am J Vet Res |
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38 |
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6 |
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735-737 |
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visual system function; Ocular Physiology |
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The equine eye was treated as a general lens system and calculations were done to determine image position in relation to the retina for objects at a distance of infinity, 100 m, and 1 m. The retina is 19.1 mm behind the posterior surface of the lens; therefore, the image appears 14.6 mm posterior to the retina at infinity and at 100 m, and 16.3 mm at 1-m distance on a horizontal axis. The animals studied were hyperopic. It is evident that the horse must move its head or eye, or both, for optimal visual acuity. At the same time, some objects in the total field of vision are imperceptible or indistinct. |
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00029645 (Issn) |
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Cited By (since 1996): 1; Export Date: 15 May 2007; Source: Scopus; Language of Original Document: English; Correspondence Address: Knill, L.M. |
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852 |
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Parry, B.W.; Gay, C.C.; McCarthy, M.A. |
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Influence of head height on arterial blood pressure in standing horses |
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1980 |
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American Journal of Veterinary Research |
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41 |
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10 |
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1626-1631 |
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Cited By (since 1996): 4; Export Date: 21 October 2008 |
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Equine Behaviour @ team @ |
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4539 |
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McDonnell, S.M.; Freeman, D.A.; Cymbaluk, N.F.; Schott, H.C. 2nd; Hinchcliff, K.; Kyle, B. |
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Behavior of stabled horses provided continuous or intermittent access to drinking water |
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1999 |
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American Journal of Veterinary Research |
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Am J Vet Res |
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60 |
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11 |
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1451-1456 |
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Aggression; Animal Husbandry; Animals; Behavior, Animal/*physiology; Drinking Behavior; Feeding Behavior; Female; Horses/*physiology/*psychology; Housing, Animal; Pregnancy; Pregnancy, Animal/physiology/*psychology; Social Behavior; Videotape Recording; *Water Supply |
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OBJECTIVE: To compare quantitative measures and clinical assessments of behavior as an indication of psychologic well-being of stabled horses provided drinking water continuously or via 1 of 3 intermittent delivery systems. ANIMALS: 22 Quarter Horse (QH) or QH-crossbred mares and 17 Belgian or Belgian-crossbred mares (study 1) and 24 QH or QH-crossbred mares and 18 Belgian or Belgian-crossbred mares (study 2). PROCEDURE: Stabled horses were provided water continuously or via 1 of 3 intermittent water delivery systems in 2 study periods during a 2-year period. Continuous 24-hour videotaped samples were used to compare quantitative measures and clinical assessments of behavior among groups provided water by the various water delivery systems. RESULTS: All horses had clinically normal behavior. Significant differences in well being were not detected among groups provided water by the various delivery systems. CONCLUSIONS AND CLINICAL RELEVANCE: Various continuous and intermittent water delivery systems can provide adequately for the psychologic well-being of stabled horses. |
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New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square 19348, USA |
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0002-9645 |
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PMID:10566826 |
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refbase @ user @ |
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1928 |
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