Abstract: The aim of this study was to test the existence of a “reactivity-to-humans” trait which is stable over time and across situations. For this purpose, four test situations were repeated on the same animals at 8 months, 1.5 years (N = 110 horses) and 2.5 years of age (N = 54 horses). These situations involved a “familiar passive human test” during which a familiar person stayed motionless in the test pen, a “familiar active human test” and an “unfamiliar active human test” during which a familiar or unfamiliar person attempted to touch the horse, and a “halter-fitting and heart-rate measurement test”, during which the horse was haltered and equipped with a surcingle and then had its heart-rate measured. Many behavioural variables were recorded during the tests, and correlations between ages and variables measured during other situations were investigated. When correlations were found, we concluded that the variables corresponded to temperament traits which are stable over time and across situations. The horses tested were divided into four groups according to breed (Anglo-Arab and Welsh) and year of birth (2001 and 2002). Data for each group were analysed separately with Spearman rank correlations. Regarding stability over time, there was a strong correlation between ages in the frequency of licking/nibbling the passive human, the time taken by a familiar or unfamiliar person to touch the horse, and the time taken to fit a halter (e.g. the time taken to touch the horse's muzzle was significantly correlated between ages in 7 out of the 8 possible cases: 0.40 <= R <= 0.67). Regarding stability across situations, results indicate that the more frequently a horse sniffed, licked or nibbled a passive human, the easier it was for either a familiar or unfamiliar active human to touch and halter it. Moreover, a horse which could be touched easily by a familiar human could also be touched and haltered easily by an unfamiliar human. These behaviours, which are linked directly to humans, therefore indicate good stability over time and across situations. In conclusion, this stability suggests the existence of a “reactivity-to-humans” temperament trait, whether the human is passive or active, familiar or unfamiliar. When the animals do not have frequent contact with humans, this reactivity is stable over time and can be measured as early as 8 months of age using the behavioural variables cited above.

Abstract: The horse's temperament, including its manageability and reactivity and/or fearfulness, is of importance as it can result in problems and can render horses unsuitable for inexperienced riders. Early experience, including handling during infancy, may influence the horse's adult behaviour and reduce its fear of humans and other potentially frigthening situations. In the various species studied, handling has generally been undertaken during the neonatal period. The aim of the present study was to test the effects of handling young horses around the time of weaning, a period which has been demonstrated to be effective in increasing ease of handling in cattle and goats. Sixteen Anglo-Arab foals were handled for 12 days either immediately following weaning (early handled: EH) or 21 days later (late handled: LH); eight additional non-handled foals served as controls (C). Handling consisted of haltering, gently petting all parts of the body, picking up feet and leading the foal over 120 m. During handling sessions, EH were easier to handle than LH: time taken to fit them with a halter, to pick up feet, and “walk-ratio” (time walking under constraint/total time walking) were significantly lower for EH. During subsequent tests conducted over 2 days, 4, and 7 months, as well as 10 months and to some extent 18 months after the end of handling period, EH and LH were easier to handle and less reactive than controls, although differences diminished with time. The period following weaning can therefore be qualified as an “optimal period” for handling. Some of the effects persist for at least 18 months.

Abstract: Four methods of ranking horses on reactivity were evaluated and compared: isolation from conspecifics, presentation of a static novel stimulus, traversing a novel stimulus in a runway (isolation, novel stimulus and runway tests, respectively) and assigning subjective emotionality scores. In all tests, horses' heart rates were recorded and behaviour was videotaped. To be considered a valid test of reactivity, at least one heart rate and one behavioural measurement in the test had to change significantly between treatments (tranquilizer administation versus sham tranquilizer administration), and behavioural measures had to be displayed in at least 75% of the trials. Forty horses performed each of the three tests daily on three different days in a switchback design. Horses were assigned randomly to a daily test sequence, which was maintained throughout the study. In the runway test, no significant difference in heart rate values in tranquilized and non-tranquilized horses was found, and no behavioural attribute was displayed in more than 52% of the trials; therefore it was rejected as a valid test of reactivity. Both isolation and novel stimulus tests produced valid measurements. Mean heart rate was the most precise physiological measure for these tests, and walking and defecation frequency were the most precise behavioural measures for novel stimulus and isolation tests, respectively. Mean heart rates on the novel stimulus and isolation tests were correlated (rs = 0.79, P < 0.01) indicating that these tests produced similar rankings based on physiological responses. However, behavioural measures ranked horses differently (rs = 0.27, P < 0.10) on the tests. Rank correlations between mean heart rates and behavioural measures were higher in the novel stimulus (rs = 0.66, P < 0.01) than the isolation test (rs = 0.55, P < 0.01), indicating that the novel stimulus test ranked horses based on either physiological or behavioural responses more similarly than did the isolation test. Therefore, the novel stimulus test was considered the more accurate evaluation of reactivity. Subjective emotionality scores were correlated moderately with mean heart rates (rs > 0.33, P < 0.01) from the novel stimulus and isolation tests and with walking scores (rs = 0.47, P < 0.01) from the novel stimulus test. Assignment of subjective emotionality scores was not as accurate as the novel stimulus or isolation tests in ranking horses for reactivity. Using physiological data alone, combining physiological and behavioural measurements or using more than one behavioural measurement in reactivity tests may reflect the reactivity of the horse better than a single behavioural measurement.

Abstract: We investigated 8 horses in five therapeutic riding centres situated in San Cataldo (Caltanissetta – I), Nicosia (Catania – II), Pellaro (Reggio Calabria – III), San Gregorio (Catania – IV), Niguarda Hospitals (Milan – V). The managements of the animals were of different typologies: Type 1, Type 2 and Type 3. In type 1 the horses were used for therapeutic riding only. Furthermore intra and interspecific social interactions were not allowed. In type 2 the horses played kinetic activities and made social interactions. In type 3 the horses were free in paddock, as intra and interspecific social interactions were allowed. The centre I, with a management of type 1, housed 1 horse (A1); the centre II, with a management of type 2, housed 1 horse (B2); the centre III, with a management of type 1, housed 1 horse (C1); the centre IV, with a management of type 2, housed 2 horses (D2 and E2); the centre V, with a management of type 3, housed 3 horses (F3, G3 and H3). Breeds of horses were: Anglo-Arab (n°1), Avelignese (n°3), Italian Selle (n°3), draught-horse crossbreed (n°1). They were 2 geldings and 6 females. Their ages ranged from 12 to 23 years. We observed a total of 64 patients affected by different pathologies: autism, motory handicap, blindness and deafness, children“s cerebral paralysis, relational problems, mental deficiency, Down”s syndrome.
The horses" behaviour was observed at rest and during therapeutic activities with these patients. The Heart Rate (HR) was used as physiological parameter for an ethological evaluation, measured by a telemetric heart rate monitor (Polar Horse Trainer). Horses were analysed with a reactivity test for emotional homeostasis evaluation, too. Heart rate values were studied with non parametrical statistical analysis methods.
Distinct management typologies provided statistically different basal mean values of heart rate (intergroup and intragroup): Type 1 vs Type 2 (P~0.05) and Type 1 vs Type 3 (P<0.05). The comparison of heart rate during therapeutic activities of diverse management showed the following results: A1 vs B2 (P<0.05), B2 vs C1 (P<0.05), Type 1 vs Type 2 (P<0.01). Different managements, both in the same or different typologies, gave significantly diverse results (A1 vs E2: P~0.05; C1 vs E2: P~0.05; B2 vs E2: P~0.05).
This study shows that the statistic differences obtained by therapies with autistic patients derive from management conditions of Type 1. In reactivity test there aren"t any significant differences among the three management typologies. However, we recorded strong variation between medium and maximum values of heart rate, especially in Type 1 and Type 2 of management.
These high variations of heart rate indicated fear reaction of the horse to new stimuli.
This reaction could lead to dangerous accidents for patients during therapeutic activities.
Horses used in therapeutic riding programs must be evaluated before this employment. Horse's behaviour can be assessed by an ethological observation and a reactivity test. Furthermore, the horses must be guaranteed welfare conditions and must live in an environment enriched with sensorial stimuli and respectful of their physiological and ethological needs.