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Author |
Permyakov, S.E.; Khokhlova, T.I.; Nazipova, A.A.; Zhadan, A.P.; Morozova-Roche, L.A.; Permyakov, E.A. |
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Title |
Calcium-binding and temperature induced transitions in equine lysozyme: new insights from the pCa-temperature “phase diagrams” |
Type |
Journal Article |
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Year |
2006 |
Publication |
Proteins |
Abbreviated Journal |
Proteins |
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Volume |
65 |
Issue  |
4 |
Pages |
984-998 |
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Keywords |
Animals; Apoproteins/chemistry/metabolism; Binding Sites; Calcium/chemistry/*metabolism; Cattle; Edetic Acid/metabolism; Horses/metabolism; Hydrogen-Ion Concentration; Lactalbumin/chemistry/metabolism; Muramidase/*chemistry/*metabolism; Protein Denaturation; Spectrometry, Fluorescence; *Temperature; Thermodynamics; Tryptophan/chemistry/metabolism |
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Abstract |
The most universal approach to the studies of metal binding properties of single-site metal binding proteins, i.e., construction of a “phase diagram” in coordinates of free metal ion concentration-temperature, has been applied to equine lysozyme (EQL). EQL has one relatively strong calcium binding site and shows two thermal transitions, but only one of them is Ca(2+)-dependent. It has been found that the Ca(2+)-dependent behavior of the low temperature thermal transition (I) of EQL can be adequately described based upon the simplest four-states scheme of metal- and temperature-induced structural changes in a protein. All thermodynamic parameters of this scheme were determined experimentally and used for construction of the EQL phase diagram in the pCa-temperature space. Comparison of the phase diagram with that for alpha-lactalbumin (alpha-LA), a close homologue of lysozyme, allows visualization of the differences in thermodynamic behavior of the two proteins. The thermal stability of apo-EQL (transition I) closely resembles that for apo-alpha-LA (mid-temperature 25 degrees C), while the thermal stabilities of their Ca(2+)-bound forms are almost indistinguishable. The native state of EQL has three orders of magnitude lower affinity for Ca(2+) in comparison with alpha-LA while its thermally unfolded state (after the I transition) has about one order lower (K = 15M(-1)) affinity for calcium. Circular dichroism studies of the apo-lysozyme state after the first thermal transition show that it shares common features with the molten globule state of alpha-LA. |
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Institute for Biological Instrumentation of the Russian Academy of Sciences, Pushchino, Moscow region 142290, Russia |
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1097-0134 |
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PMID:17022083 |
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Call Number |
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Serial |
1858 |
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Author |
Dallmeyer, M.D.; Turner, R.M.; McDonnell, S.M.; Sertich, P.L.; Dolente, B.A.; Parente, E.J.; Diaz, O.M.S. |
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Title |
Theriogenology question of the month. Behavior problems in a stallion caused by a nephrolith |
Type |
Journal Article |
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Year |
2006 |
Publication |
Journal of the American Veterinary Medical Association |
Abbreviated Journal |
J Am Vet Med Assoc |
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Volume |
229 |
Issue |
4 |
Pages |
511-513 |
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Keywords |
Animals; Behavior, Animal; Horse Diseases/physiopathology/surgery/*ultrasonography; Horses/*physiology; Kidney Calculi/physiopathology/surgery/ultrasonography/*veterinary; Male; Sperm Count/*veterinary; *Sperm Motility; Treatment Outcome |
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Address |
Department of Clinical Studies, New Bolton Center, School of Veterinary Medicine, University of Pennsylvania, Kennett Square, PA 19348, USA |
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Corporate Author |
American College of Theriogenologists |
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0003-1488 |
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PMID:16910847 |
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Serial |
1865 |
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Ionita, J.C.; Poncet, P.A.; Doherr, M.G.; Steiger, A. |
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Title |
[Evaluation of the quality of husbandry of Franches-Montagnes horses in their breeding farms] |
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Journal Article |
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Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
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Volume |
148 |
Issue |
4 |
Pages |
191-197 |
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Keywords |
Animal Husbandry/methods/*standards/statistics & numerical data; *Animal Welfare; Animals; Breeding/*methods/standards; Female; Floors and Floorcoverings; Horses/*physiology; Housing, Animal/*standards; Male; Poaceae; Questionnaires; Social Behavior; Switzerland |
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Abstract |
The quality of husbandry of Franches-Montagnes horses (FM) in Switzerland is evaluated on the basis of an investigation carried out in 2002 by the Swiss FM breeding federation. Questionnaires were sent to 3500 of its members and the results include data from 968 breeding enterprises, housing a total of 3965 FM: 46.1% were breeding mares (61.0% with foal at foot), 26.5% young stock, 1.3% stallions and 26.0% non breeding stock (74.6% of which were pleasure horses and 25.4% working horses). 57.6% of the FM were housed in individual boxes with or without permanent outdoor access, 25.4% were hold in groups with or without permanent outdoor access, the remaining 17.0% were kept in standing stalls. 95.0% of the FM had at least visual contact with other equines and 99.2% had sufficient light in their stable. 88.1% were stabled on long stalk straw, while only 4.3% were bedded on other materials other than straw. The average time spent at pasture per horse and per week ranged from 96.5 +/- 51.6 hours in summer to 27.2 +/- 26.7 hours in winter. On average, a FM is used for 8.3 +/- 6.5 hours per week. Horses with an paddock at their disposal spend an average of 39.8 +/- 45.9 hours there per week. |
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Chirurgische Tierklinik, Universitat Leipzig |
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French |
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Original Title |
Les conditions de detention des chevaux de race franches-montagnes dans leurs exploitations d'elevage |
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0036-7281 |
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Notes |
PMID:16703760 |
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no |
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Call Number |
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Serial |
1872 |
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Author |
Poncet, P.A.; Ionita, J.C.; Doherr, M.G.; Steiger, A. |
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Title |
[The influence of the socio-economic structure of the breeding farms of Franches-Montagnes horses on the conditions of husbandry] |
Type |
Journal Article |
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Year |
2006 |
Publication |
Schweizer Archiv fur Tierheilkunde |
Abbreviated Journal |
Schweiz Arch Tierheilkd |
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Volume |
148 |
Issue |
4 |
Pages |
183-189 |
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Keywords |
Altitude; Animal Husbandry/methods/*standards; Animals; Breeding/*economics/standards; Demography; Female; Horses/*physiology; Housing, Animal/*standards; Male; Physical Conditioning, Animal/methods/*physiology; Questionnaires; Social Behavior; Socioeconomic Factors; Switzerland |
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Abstract |
The socio-economic structure of the breeding farms of Franches-Montagnes horses (FM) in Switzerland is evaluated on the basis of an investigation carried out in 2002 by the Swiss FM breeding federation. Questionnaires were sent to 3500 of its members and the results include data from 968 breeding enterprises, housing a total of 3965 FM. The quality of the husbandry of FM varies according to factors such as the altitude and the geographical situation of the farms and studs. Socio-economic parameters, such as the role of FM in the business, their use (breeding, driving, riding) and the age and level of professional education of the owners may also have an effect on standards of husbandry. The results show that the owners for whom FM represent a source of income more frequently keep their horses in standing stalls, but give them more time to exercise at liberty than the horses belonging to amateur breeders. Younger and better educated breeders are more likely to house their animals in groups. |
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Address |
Haras national suisse, Avenches |
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French |
Summary Language |
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Original Title |
L'influence de la structure socio-economique des exploitations d'elevage de chevaux de race franches-montagnes sur les conditions de detention |
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0036-7281 |
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Notes |
PMID:16703759 |
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no |
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Serial |
1873 |
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Author |
Piccione, G.; Caola, G.; Refinetti, R. |
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Title |
Temporal relationships of 21 physiological variables in horse and sheep |
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Journal Article |
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Year |
2005 |
Publication |
Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology |
Abbreviated Journal |
Comp Biochem Physiol A Mol Integr Physiol |
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Volume |
142 |
Issue |
4 |
Pages |
389-396 |
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Keywords |
Animals; Behavior, Animal/physiology; Blood Glucose/physiology; Body Temperature/*physiology; Circadian Rhythm/*physiology; Female; Horses/*physiology; Melatonin/blood/*physiology; Motor Activity/*physiology; Rectum/physiology; Sheep/*physiology; Time Factors |
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Abstract |
Daily or circadian oscillation has been documented in a variety of physiological and behavioral processes. Although individual variables have been studied in great detail, very few studies have been conducted on the temporal relationships between the rhythms of different variables. It is not known whether the circadian pacemaker generates each and every rhythm individually or whether most rhythms are simply derived from a few clock-controlled rhythms. As a first step in elucidating this issue, 21 physiological variables were recorded simultaneously in horse and sheep. The results indicated that, in both species, different variables exhibit different degrees of daily rhythmicity and reach their daily peaks at different times of the day. The variables exhibiting strongest rhythmicity were locomotor activity, rectal temperature, and plasma concentrations of melatonin and glucose. Comparison of rhythmicity and acrophase in the various rhythms allowed inferences to be made about mechanisms of causation. |
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Dipartimento di Morfologia, Biochimica, Fisiologia e Produzioni Animali, Facolta di Medicina Veterinaria, Universita degli Studi di Messina, 98168 Messina, Italy |
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English |
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ISSN |
1095-6433 |
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Notes |
PMID:16290083 |
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no |
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Call Number |
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Serial |
1884 |
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Author |
Husted, L.; Andersen, M.S.; Borggaard, O.K.; Houe, H.; Olsen, S.N. |
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Title |
Risk factors for faecal sand excretion in Icelandic horses |
Type |
Journal Article |
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Year |
2005 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
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Volume |
37 |
Issue |
4 |
Pages |
351-355 |
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Keywords |
Animal Feed; Animal Husbandry/methods; Animals; Denmark; Feces/*chemistry; Female; Gastrointestinal Diseases/epidemiology/etiology/prevention & control/veterinary; Horse Diseases/epidemiology/etiology/prevention & control; Horses/*metabolism; Logistic Models; Male; Pilot Projects; *Poaceae/growth & development; Risk Factors; Silicon Dioxide/administration & dosage/adverse effects/*analysis; Soil/*analysis |
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Abstract |
REASONS FOR PERFORMING STUDY: Sandy soil is often mentioned as a risk factor in the development of sand-related gastrointestinal disease (SGID) in the horse. There are other variables, but few studies confirm any of these. OBJECTIVE: To investigate soil type, pasture quality, feeding practice in the paddock, age, sex and body condition score as risk factors for sand intake in the horse. METHODS: Faeces were collected from 211 Icelandic horses on 19 different studs in Denmark together with soil samples and other potential risk factors. Sand content in faeces determined by a sand sedimentation test was interpreted as evidence of sand intake. Soil types were identified by soil analysis and significance of the data was tested using logistic analysis. RESULTS: Of horses included in the study, 56.4% showed sand in the faeces and 5.7% had more than 5 mm sand as quantified by the rectal sleeve sedimentation test. Soil type had no significant effect when tested as main effect, but there was interaction between soil type and pasture quality. Significant interactions were also found between paddock feeding practice and pasture quality. CONCLUSION: To evaluate the risk of sand intake it is important to consider 3 variables: soil type, pasture quality and feeding practice. Pasture quality was identified as a risk factor of both short and long grass in combination with sandy soil, while clay soil had the lowest risk in these combinations. Feeding practice in the paddock revealed feeding directly on the ground to be a risk factor when there was short (1-5 cm) or no grass. Also, no feeding outdoors increased the risk on pastures with short grass, while this had no effect in paddocks with no grass. More than 50% of all horses investigated in this study had sand in the faeces. POTENTIAL RELEVANCE: The identification of risk factors is an important step towards prevention of SGID. Further research is necessary to determine why some horses exhibit more than 5 mm sand in the sedimentation test and whether this is correlated with geophagic behaviour. |
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Address |
Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlaegevej 88, DK-1870 Frederiksberg, Denmark |
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English |
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ISSN |
0425-1644 |
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Notes |
PMID:16028626 |
Approved |
no |
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Call Number |
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Serial |
1888 |
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Author |
Hausberger, M.; Bruderer, C.; Le Scolan, N.; Pierre, J.-S. |
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Title |
Interplay between environmental and genetic factors in temperament/personality traits in horses (Equus caballus) |
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Journal Article |
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Year |
2004 |
Publication |
Journal of Comparative Psychology |
Abbreviated Journal |
J Comp Psychol |
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Volume |
118 |
Issue |
4 |
Pages |
434-446 |
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Keywords |
*Affect; Animals; Behavior, Animal/*physiology; *Environment; Female; Horses/*psychology; Learning; Male; Memory/physiology |
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Abstract |
The aim of the present study was to broach the question of the relative influence of different genetic and environmental factors on different temperament/personality traits of horses (Equus caballus). The researchers submitted 702 horses to standardized experimental tests and investigated 9 factors, either genetic or environmental. Genetic factors, such as sire or breed, seemed to influence more neophobic reactions, whereas environmental factors, such as the type of work, seemed to play a more dominant role in reactions to social separation or learning abilities. Additive effects were evident, showing how environmental factors may modulate behavioral traits. This study constitutes a first step toward understanding the relative weights of genetic factors and how the environment may intervene in determining individual behavioral characteristics. |
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Address |
Ethologie-Evolution-Ecologie, Universite de Rennes 1, Rennes, France. Martine.Hausberger@univ-rennes1.fr |
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Place of Publication |
Washington, D.C. : 1983 |
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English |
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ISSN |
0735-7036 |
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Notes |
PMID:15584780 |
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no |
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Call Number |
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Serial |
1897 |
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Author |
Natalini, C.C.; Robinson, E.P. |
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Title |
Effects of epidural opioid analgesics on heart rate, arterial blood pressure, respiratory rate, body temperature, and behavior in horses |
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Journal Article |
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Year |
2003 |
Publication |
Veterinary Therapeutics : Research in Applied Veterinary Medicine |
Abbreviated Journal |
Vet Ther |
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Volume |
4 |
Issue |
4 |
Pages |
364-375 |
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Keywords |
3,4-Dichloro-N-methyl-N-(2-(1-pyrrolidinyl)-cyclohexyl)-benzeneacetamide, (trans)-Isomer/administration & dosage/pharmacology; Alfentanil/administration & dosage/pharmacology; Analgesics, Opioid/administration & dosage/*pharmacology; Anesthesia, Epidural/*veterinary; Animals; Behavior, Animal/drug effects; Blood Pressure/drug effects; Body Temperature/drug effects; Butorphanol/administration & dosage/pharmacology; Cross-Over Studies; Female; Heart Rate/drug effects; Horses/*physiology; Injections, Epidural/veterinary; Male; Morphine/administration & dosage/pharmacology; Respiration/drug effects; Tramadol/administration & dosage/pharmacology |
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Abstract |
Heart rate, arterial blood pressures, respiratory rate, body temperature, and central nervous system excitement were compared before and after epidural administration of morphine (0.1 mg/kg), butorphanol (0.08 mg/kg), alfentanil (0.02 mg/kg), tramadol (1.0 mg/kg), the k-opioid agonist U50488H (0.08 mg/kg), or sterile water using an incomplete Latin square crossover design in five conscious adult horses. Treatments were administered into the first intercoccygeal epidural space. Significant (P <.05) reductions in respiratory rate were detected after epidural administration of morphine, alfentanil, U50488H, and sterile water. Additionally, significant (P <.05) head ptosis was observed within the first hour after administration of morphine, U50488H, and tramadol, but neither of these changes appeared to be of clinical significance. No treatment-related changes in motor activity or behavior were observed. |
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Address |
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA |
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English |
Summary Language |
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ISSN |
1528-3593 |
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Notes |
PMID:15136978 |
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no |
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Call Number |
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Serial |
1902 |
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Author |
Shanahan, S. |
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Title |
Trailer loading stress in horses: behavioral and physiological effects of nonaversive training (TTEAM) |
Type |
Journal Article |
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Year |
2003 |
Publication |
Journal of Applied Animal Welfare Science : JAAWS |
Abbreviated Journal |
J Appl Anim Welf Sci |
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Volume |
6 |
Issue |
4 |
Pages |
263-274 |
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Keywords |
Animals; *Conditioning, Operant; *Escape Reaction; Female; Heart Rate; Horses/*psychology; Hydrocortisone/metabolism; Male; Saliva/metabolism; Stress/metabolism/prevention & control/*veterinary; *Transportation |
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Abstract |
Resistance in the horse to trailer loading is a common source of stress and injury to horses and their handlers. The objective of this study was to determine whether nonaversive training based on the Tellington-Touch Equine Awareness Method (TTEAM; Tellington-Jones &Bruns, 1988) would decrease loading time and reduce stress during loading for horses with a history of reluctance to load. Ten horses described by their owners as “problem loaders” were subjected to pretraining and posttraining assessments of loading. Each assessment involved two 7-min loading attempts during which heart rate and saliva cortisol were measured. The training consisted of six 30-min sessions over a 2-week period during which the horse and owner participated in basic leading exercises with obstacles simulating aspects of trailering. Assessment showed heart rate and saliva cortisol increased significantly during loading as compared to baseline (p <.001 and p <.05, respectively). Reassessment after training showed a decrease in loading time (p <.02), reduced heart rate during loading (p <.002), and reduced saliva cortisol as compared to pretraining assessments. Seven “good loaders” also were subject to loading assessment for physiological comparison. Increases in heart rate during loading were significantly higher in the good loaders (p <.001). Nonaversive training simulating aspects of loading may effectively reduce loading time and stress during loading for horses with a history of resistance to trailer loading. |
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Address |
shanahandvm@yahoo.ca |
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English |
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Edition |
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ISSN |
1088-8705 |
ISBN |
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Notes |
PMID:14965781 |
Approved |
no |
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Call Number |
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Serial |
1903 |
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Author |
Ferguson, D.L.; Rosales-Ruiz, J. |
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Title |
Loading the problem loader: the effects of target training and shaping on trailer-loading behavior of horses |
Type |
Journal Article |
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Year |
2001 |
Publication |
Journal of Applied Behavior Analysis |
Abbreviated Journal |
J Appl Behav Anal |
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Volume |
34 |
Issue |
4 |
Pages |
409-423 |
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Keywords |
Animals; *Conditioning, Operant; *Escape Reaction; Female; Horses/*psychology; Reinforcement (Psychology); *Transportation |
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Abstract |
The purpose of this study was to develop an effective method for trailer loading horses based on principles of positive reinforcement. Target training and shaping were used to teach trailer-loading behavior to 5 quarter horse mares in a natural setting. All 5 had been trailer loaded before through the use of aversive stimulation. Successive approximations to loading and inappropriate behaviors were the dependent variables. After training a horse to approach a target, the target was moved to various locations inside the trailer. Horses started training on the left side of a two-horse trailer. After a horse was loading on the left side, she was moved to the right side, then to loading half on the right and half on the left. A limited-hold procedure and the presence of a companion horse seemed to facilitate training for 1 horse. Inappropriate behaviors fell to zero immediately after target training, and all the horses successfully completed the shaping sequence. Finally, these effects were observed to generalize to novel conditions (a different trainer and a different trailer). |
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Address |
University of North Texas, Denton 76203, USA |
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English |
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Series Issue |
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Edition |
|
|
| |
ISSN |
0021-8855 |
ISBN |
|
Medium |
|
|
| |
Area |
|
Expedition |
|
Conference |
|
|
| |
Notes |
PMID:11800182 |
Approved |
no |
|
| |
Call Number |
refbase @ user @ |
Serial |
1915 |
|
| Permanent link to this record |
