| Records |
| Author |
Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B. |
| Title |
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces |
Type |
Journal Article |
| Year |
2004 |
Publication |
Parasitology |
Abbreviated Journal |
Parasitology |
| Volume |
129 |
Issue  |
Pt 6 |
Pages |
733-739 |
| Keywords |
Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics |
| Abstract |
Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae. |
| Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it |
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English |
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Edition |
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| ISSN |
0031-1820 |
ISBN |
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Conference |
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| Notes |
PMID:15648696 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2631 |
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| Author |
Dutto, D.J.; Hoyt, D.F.; Clayton, H.M.; Cogger, E.A.; Wickler, S.J. |
| Title |
Moments and power generated by the horse (Equus caballus) hind limb during jumping |
Type |
Journal Article |
| Year |
2004 |
Publication |
The Journal of Experimental Biology |
Abbreviated Journal |
J Exp Biol |
| Volume |
207 |
Issue |
Pt 4 |
Pages |
667-674 |
| Keywords |
Animals; Biomechanics; Hindlimb/*physiology; Horses/*physiology; Locomotion/*physiology |
| Abstract |
The ability to jump over an obstacle depends upon the generation of work across the joints of the propelling limb(s). The total work generated by one hind limb of a horse and the contribution to the total work by four joints of the hind limb were determined for a jump. It was hypothesized that the hip and ankle joints would have extensor moments performing positive work, while the knee would have a flexor moment and perform negative work during the jump. Ground reaction forces and sagittal plane kinematics were simultaneously recorded during each jumping trial. Joint moment, power and work were determined for the metatarsophalangeal (MP), tarsal (ankle), tibiofemoral (knee) and coxofemoral (hip) joints. The hip, knee and ankle all flexed and then extended and the MP extended and then flexed during ground contact. Consistent with our hypothesis, large extensor moments were observed at the hip and ankle joints and large flexor moments at the knee and MP joints throughout ground contact of the hind limb. Peak moments tended to occur earlier in stance in the proximal joints but peak power generation of the hind limb joints occurred at similar times except for the MP joint, with the hip and ankle peaking first followed by the MP joint. During the first portion of ground contact (approximately 40%), the net result of the joint powers was the absorption of power. During the remainder of the contact period, the hind limb generated power. This pattern of power absorption followed by power generation paralleled the power profiles of the hip, ankle and MP joints. The total work performed by one hind limb was 0.71 J kg(-1). Surprisingly, the knee produced 85% of the work (0.60 J kg(-1)) done by the hind limb, and the positive work performed by the knee occurred during the first 40% of the take-off. There is little net work generated by the other three joints over the entire take-off. Velocity of the tuber coxae (a landmark on the pelvis of the animal) was negative (downward) during the first 40% of stance, which perhaps reflects the negative work performed to decrease the potential energy during the first 40% of contact. During the final 60% of contact, the hip, ankle and MP joints generate positive work, which is reflected in the increase of the animal's potential energy. |
| Address |
Department of Kinesiology and Health Promotion, California State Polytechnic University, Pomona, CA 91768, USA. ddutto@csupomona.edu |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0022-0949 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:14718509 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3654 |
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| Author |
Witte, T.H.; Knill, K.; Wilson, A.M. |
| Title |
Determination of peak vertical ground reaction force from duty factor in the horse (Equus caballus) |
Type |
Journal Article |
| Year |
2004 |
Publication |
The Journal of Experimental Biology |
Abbreviated Journal |
J Exp Biol |
| Volume |
207 |
Issue |
Pt 21 |
Pages |
3639-3648 |
| Keywords |
*Acceleration; Animals; Biomechanics; Forelimb/physiology; *Gait; Hindlimb/physiology; Horses/*physiology; Locomotion/*physiology; Telemetry; Time Factors |
| Abstract |
Measurement of peak vertical ground reaction force (GRFz) from multiple limbs simultaneously during high-speed, over-ground locomotion would enhance our understanding of the locomotor mechanics of cursorial animals. Here, we evaluate the accuracy of predicting peak GRFz from duty factor (the proportion of the stride for which the limb is in contact with the ground). Foot-mounted uniaxial accelerometers, combined with UHF FM telemetry, are shown to be practical and accurate for the field measurement of stride timing variables, including duty factor. Direct comparison with the force plate produces a mean error of 2.3 ms and 3.5 ms for the timing of foot on and foot off, respectively, across all gaits. Predictions of peak GRFz from duty factor show mean errors (with positive values indicating an overestimate) of 0.8+/-0.04 N kg(-1) (13%; N=42; mean +/- S.E.M.) at walk, -0.3+/-0.06 N kg(-1) (3%; N=75) at trot, -2.3+/-0.27 N kg(-1) (16%; N=18) for the non-lead limb at canter and +2.1+/-0.7 N kg(-1) (19%; N=9) for the lead limb at canter. The substantial over- and underestimate seen at canter, in the lead and non-lead limbs, respectively, is attributed to the different functions performed by the two limbs in the asymmetrical gaits. The difference in load experienced by the lead and non-lead limbs decreased with increasing speed. |
| Address |
Structure and Motion Lab, The Royal Veterinary College, Hawkshead Lane, Hatfield, Hertfordshire, AL9 7TA, UK |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0022-0949 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:15371472 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3658 |
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| Author |
Nicol, C.J.; Yoon, M.; Ward, J.M.; Yamashita, M.; Fukamachi, K.; Peters, J.M.; Gonzalez, F.J. |
| Title |
PPARgamma influences susceptibility to DMBA-induced mammary, ovarian and skin carcinogenesis |
Type |
Journal Article |
| Year |
2004 |
Publication |
Carcinogenesis |
Abbreviated Journal |
Carcinogenesis |
| Volume |
25 |
Issue |
9 |
Pages |
1747-1755 |
| Keywords |
9,10-Dimethyl-1,2-benzanthracene/*toxicity; Animals; DNA Primers/chemistry; Disease Susceptibility; Female; Heterozygote; Humans; Mammary Neoplasms, Experimental/chemically induced/*pathology; Mice; Ovarian Neoplasms/chemically induced/*pathology; RNA, Messenger/genetics/metabolism; Receptors, Cytoplasmic and Nuclear/genetics/*physiology; Reverse Transcriptase Polymerase Chain Reaction; Skin Neoplasms/chemically induced/*pathology; Survival Rate; Transcription Factors/genetics/*physiology; Zinc Fingers |
| Abstract |
Peroxisome proliferator-activated receptor gamma (PPARgamma), a member of the nuclear receptor superfamily, plays a role in adipocyte differentiation, type II diabetes, macrophage response to inflammation and is suggested to influence carcinogen-induced colon cancer. Studies done in vitro and in vivo also revealed that PPARgamma ligands might promote differentiation and/or regression of mammary tumors. To directly evaluate the role of PPARgamma in mammary carcinogenesis, PPARgamma wild-type (+/+) or heterozygous (+/-) mice were administered 1 mg 7,12-dimethylbenz[a]anthracene (DMBA) by gavage once a week for 6 weeks and followed for a total of 25 weeks. Compared with congenic PPARgamma(+/+) littermate controls, PPARgamma(+/-) mice had early evidence for increased susceptibility to DMBA-mediated carcinogenesis based on a 1.6-fold increase in the percentage of mice with skin papillomas, as well as a 1.7-fold increase in the numbers of skin papillomas per mouse (P < 0.05). Similarly, PPARgamma(+/-) mice also had a 1.5-fold decreased survival rate (P = 0.059), and a 1.7-fold increased incidence of total tumors per mouse (P < 0.01). Moreover, PPARgamma(+/-) mice had an almost 3-fold increase in mammary adenocarcinomas (P < 0.05), an over 3-fold increase in ovarian granulosa cell carcinomas (P < 0.05), an over 3-fold increase in malignant tumors (P < 0.02) and a 4.6-fold increase in metastatic incidence. These results are the first to demonstrate an increased susceptibility in vivo of PPARgamma haploinsufficiency to DMBA-mediated carcinogenesis and suggest that PPARgamma may act as a tumor modifier of skin, ovarian and breast cancers. The data also support evidence suggesting a beneficial role for PPARgamma-specific ligands in the chemoprevention of mammary, ovarian and skin carcinogenesis. |
| Address |
Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, Bethesda, MD 20892, USA |
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English |
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Edition |
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| ISSN |
0143-3334 |
ISBN |
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| Notes |
PMID:15073042 |
Approved |
no |
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refbase @ user @ |
Serial |
76 |
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| Author |
McGreevy, P. |
| Title |
Equine behavior |
Type |
Journal Article |
| Year |
2004 |
Publication |
Journal of Equine Veterinary Science |
Abbreviated Journal |
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| Volume |
24 |
Issue |
9 |
Pages |
397-398 |
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no |
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refbase @ user @ |
Serial |
1983 |
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| Author |
Verguts, T.; Fias, W. |
| Title |
Representation of Number in Animals and Humans: A Neural Model |
Type |
Journal Article |
| Year |
2004 |
Publication |
J. Cogn. Neurosci. |
Abbreviated Journal |
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| Volume |
16 |
Issue |
9 |
Pages |
1493-1504 |
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| Abstract |
This article addresses the representation of numerical information conveyed by nonsymbolic and symbolic stimuli. In a first simulation study, we show how number-selective neurons develop when an initially uncommitted neural network is given nonsymbolic stimuli as input (e.g., collections of dots) under unsupervised learning. The resultant network is able to account for the distance and size effects, two ubiquitous effects in numerical cognition. Furthermore, the properties of the network units conform in detail to the characteristics of recently discovered number-selective neurons. In a second study, we simulate symbol learning by presenting symbolic and nonsymbolic input simultaneously. The same number-selective neurons learn to represent the numerical meaning of symbols. In doing so, they show properties reminiscent of the originally available number-selective neurons, but at the same time, the representational efficiency of the neurons is increased when presented with symbolic input. This finding presents a concrete proposal on the linkage between higher order numerical cognition and more primitive numerical abilities and generates specific predictions on the neural substrate of number processing. N1 - |
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Approved |
no |
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Equine Behaviour @ team @ |
Serial |
2954 |
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| Author |
Blazyczek, I.; Hamann, H.; Deegen, E.; Distl, O.; Ohnesorge, B. |
| Title |
Retrospective analysis of 50 cases of guttural pouch tympany in foals |
Type |
Journal Article |
| Year |
2004 |
Publication |
The Veterinary Record |
Abbreviated Journal |
Vet. Rec. |
| Volume |
154 |
Issue |
9 |
Pages |
261-264 |
| Keywords |
Animals; Female; Germany/epidemiology; Horse Diseases/*surgery; Horses; Male; Pharyngeal Diseases/epidemiology/surgery/*veterinary; Retrospective Studies; Severity of Illness Index; Species Specificity |
| Abstract |
Between 1994 and 2001, guttural pouch tympany was diagnosed in 51 foals; there were approximately three times as many fillies as colts, of Arabian, different German warmblood breeds and Western horse breeds. There were significantly more Arabian and paint horse foals than expected in comparison with the breed distribution of the foals hospitalised at the Clinic for Horses. The foals' breed and sex did not influence the age of onset, the type and severity of the clinical signs or the recurrence rate. A surgical laser technique was used on 50 of the foals; in 35 cases only one surgical treatment was necessary, in seven cases a second operation was required during the foal's initial period of hospitalisation, and in eight cases a second operation was performed during a second period of hospitalisation. Long-term follow-up information was obtained for 44 of the 50 treated horses; 24 of them were under two years of age and 20 were over two years of age. In six horses, no follow-up information was available. Four horses were euthanased for reasons unrelated to the condition or its treatment. The horses over two years of age were in training or were being used for competitions in dressage or jumping or for breeding purposes, and in only one of them was an adventitious respiratory noise reported. All the horses up to two years of age were reported to be healthy. |
| Address |
Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Buenteweg 17p, D-30559 Hannover, Germany |
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English |
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Edition |
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| ISSN |
0042-4900 |
ISBN |
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| Notes |
PMID:15029964 |
Approved |
no |
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Equine Behaviour @ team @ |
Serial |
3719 |
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| Author |
Neuringer, A. |
| Title |
Reinforced variability in animals and people: implications for adaptive action |
Type |
Journal Article |
| Year |
2004 |
Publication |
The American Psychologist |
Abbreviated Journal |
Am Psychol |
| Volume |
59 |
Issue |
9 |
Pages |
891-906 |
| Keywords |
Animals; Behavior, Animal; *Choice Behavior; Conditioning, Operant; Creativeness; Discrimination (Psychology); Humans; Memory; Problem Solving; *Reinforcement (Psychology) |
| Abstract |
Although reinforcement often leads to repetitive, even stereotyped responding, that is not a necessary outcome. When it depends on variations, reinforcement results in responding that is diverse, novel, indeed unpredictable, with distributions sometimes approaching those of a random process. This article reviews evidence for the powerful and precise control by reinforcement over behavioral variability, evidence obtained from human and animal-model studies, and implications of such control. For example, reinforcement of variability facilitates learning of complex new responses, aids problem solving, and may contribute to creativity. Depression and autism are characterized by abnormally repetitive behaviors, but individuals afflicted with such psychopathologies can learn to vary their behaviors when reinforced for so doing. And reinforced variability may help to solve a basic puzzle concerning the nature of voluntary action. |
| Address |
Department of Psychology, Reed College, Portland, OR 97202, USA. allen.neuringer@reed.edu |
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English |
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0003-066X |
ISBN |
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| Notes |
PMID:15584823 |
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no |
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Equine Behaviour @ team @ |
Serial |
4106 |
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Thompson, R.N.; Robertson, B.K.; Napier, A.; Wekesa, K.S. |
| Title |
Sex-specific Responses to Urinary Chemicals by the Mouse Vomeronasal Organ |
Type |
Journal Article |
| Year |
2004 |
Publication |
Chem. Senses |
Abbreviated Journal |
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| Volume |
29 |
Issue |
9 |
Pages |
749-754 |
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| Abstract |
Social behaviors of most mammals are affected by chemical signals, pheromones, exchanged between conspecifics. Previous experiments have shown that behavioral responses to the same pheromone differ depending on the sex and endocrine status of the respondent. Although the exact mechanism of this dimorphism is not known, one possible contributor may be due to sexually dimorphic receptors or due to differences in central processing within the brain. In order to investigate the differences in response between male and female mice to the same pheromonal stimulus two urinary compounds (2-heptanone and 2,5-dimethylpyrazine) were used to stimulate the production of Inositol (1,4,5)-trisphosphate (IP3) in microvillar membrane preparations of the vomeronasal organ as an indirect measurement of pheromonal stimulation. Incubation of such membranes from prepubertal mice with urine from the same sex or opposite sex, results in an increase in production of IP3. This stimulation is mimicked by GTP{gamma}S and blocked by GDP{beta}S. Furthermore we found that 2-heptanone present in both male and female urine was capable of stimulating increased production of IP3 in the female VNO but not the male VNO. Finally, 2,5-dimethylpyrazine present only in female urine was also only capable of stimulating increased production of IP3 in the female VNO. |
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| Notes |
10.1093/chemse/bjh076 |
Approved |
no |
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Equine Behaviour @ team @ |
Serial |
4428 |
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Wennerstrand, J.; Johnston, C.; Roethlisberger-Holm, K.; Erichsen, C.; Eksell, P.; Drevemo, S. |
| Title |
Kinematic evaluation of the back in the sport horse with back pain |
Type |
Journal Article |
| Year |
2004 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
36 |
Issue |
8 |
Pages |
707-711 |
| Keywords |
Animals; Back/*physiology; Back Pain/diagnosis/physiopathology/*veterinary; Biomechanics; Exercise Test/veterinary; Gait/*physiology; Horse Diseases/diagnosis/*physiopathology; Horses/anatomy & histology/*physiology; Locomotion/physiology; Lumbar Vertebrae/physiology; Range of Motion, Articular; Stress, Mechanical; Thoracic Vertebrae/physiology; Weight-Bearing |
| Abstract |
REASONS FOR PERFORMING STUDY: Earlier studies have developed a clinical tool to evaluate objectively the function of the equine back. The ability to differentiate horses with back pain from asymptomatic, fully functioning horses using kinematic measures from this tool has not been evaluated. OBJECTIVES: To compare the kinematics of the back at walk and trot in riding horses with back dysfunction to the same parameters in asymptomatic sport horses. METHODS: The kinematics of the back in 12 horses with impaired performance and back pain were studied at walk and trot on a treadmill. Data were captured for 10 sees at 240 Hz. Range of movement (ROM) and intravertebral pattern symmetry of movement for flexion and extension (FE), lateral bending (LB) and axial rotation (AR) were derived from angular motion pattern data and the results compared to an earlier established database on asymptomatic riding horses. RESULTS: At walk, horses with back dysfunction had a ROM smaller for dorsoventral FE in the caudal thoracic region (T13 = 7.50 degrees, T17 = 7.71 degrees; P<0.05), greater for LB at T13 (8.13 degrees; P<0.001) and smaller for AR of the pelvis (10.97 degrees; P<0.05) compared to asymptomatic horses (FE-T13 = 8.28 degrees, FE-T17 = 8.49 degrees, LB-T13 = 6.34 degrees, AR-pelvis = 12.77 degrees). At trot, dysfunctional horses had a smaller (P<0.05) ROM for FE at the thoracic lumbar junction (T17 = 2.46 degrees, L1 = 2.60 degrees) compared to asymptomatic horses (FE-T17 = 3.07 degrees, FE-L1 = 3.12 degrees). CONCLUSIONS: The objective measurement technique can detect differences between back kinematics in riding horses with signs of back dysfunction and asymptomatic horses. The clinical manifestation of back pain results in diminished flexion/extension movement at or near the thoracic lumbar junction. However, before applying the method more extensively in practice it is necessary to evaluate it further, including measurements of patients whose diagnoses can be confirmed and long-term follow-ups of back patients after treatment. POTENTIAL RELEVANCE: Since the objective measurement technique can detect small movement differences in back kinematics, it should help to clinically describe and, importantly, objectively detect horses with back pain and dysfunction. |
| Address |
Department of Anatomy and Physiology, Swedish University of Agricultural Sciences, SE-750 07 Uppsala, Sweden |
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English |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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| Notes |
PMID:15656501 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3656 |
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