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Author Palme, R.; Touma, C.; Arias, N., Dominchin, M.N.; Lepschy, M.
Title Steroid extraction: Get the best out of faecal samples Type Journal Article
Year 2013 Publication Wiener Tierärztliche Wochenschriften Abbreviated Journal Wien Tierärztl Monat – Vet Med Austria
Volume 100 Issue Pages (down) 238-246.
Keywords Review, faeces, extraction, non-invasive hormone monitoring, stress, reproduction.
Abstract Faecal steroid hormone metabolites are becoming increasingly popular as parameters for reproductive functions and stress. The extraction of the steroids from the faecal matrix represents the initial step before quantification can be performed. The steroid metabolites present in the faecal matrix are of varying polarity and composition, so selection of a proper extraction procedure is essential. There have been some studies to address this complex but often neglected point. Radiolabelled steroids (e.g. cortisol or progesterone) have frequently been added to faecal samples to estimate the efficiency of the extraction procedures used. However, native, unmetabolized steroids are normally not present in the faeces and therefore the results are artifi- cial and do not accurately reflect the actual recoveries of the substances of interest. In this respect, recovery experiments based on faecal samples from radiometabolism studies are more informative. In these samples, the metabolite content accurately reflects the mixture of metabolites present in the given species. As a result, it is possible to evaluate different extraction methods for use with faecal samples. We present studies on sheep, horses, pigs, hares and dogs that utilized samples containing naturally metabolized, 14C-labelled steroids. We recommend extracting faecal steroids by simply suspending the faeces in a high percentage of a primary alcohol (for glucocorticoid metabolites 80% aqueous methanol proved best suited for virtually all mammalian species tested so far). Not only does the procedure significantly increase the total amount of recovered radioactivity, it also increases the percentage of unconjugated metabolites, which are more likely to be recognized by the antibodies used in various immunoassays. The advantages of this extraction procedure are clear: it is very easy to use (no evaporation step is needed), it yields high recoveries and variation based on the extraction procedure is reduced to a minimum.
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Call Number Equine Behaviour @ team @ Serial 6520
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Author Tomkins, L.M.; McGreevy, P.D.; Branson, N.J.
Title Lack of standardization in reporting motor laterality in the domestic dog (Canis familiaris) Type Journal Article
Year 2010 Publication Journal of Veterinary Behaviour Abbreviated Journal
Volume 5 Issue 5 Pages (down) 235-239
Keywords dog; motor laterality; lateralization; paw preference; standardization
Abstract Over the past 2 decades, numerous studies have been undertaken to assess motor laterality in the domestic dog. In anticipation of growth in this area of enquiry, we decided to review the literature on canine motor biases to identify any shortcomings, reflect on the lessons to be learned from and offer ways forward for future research into canine laterality. The aim of this review is to (i) summarize motor laterality findings in the dog, (ii) highlight areas lacking in standardization, and (iii) propose necessary criteria for future tests and global reporting protocols. Our review of the literature highlighted the lack of standardization between studies in task selection, sample size, number of behavior scores recorded, and the methods by which motor laterality were classified and reported. This review illustrates the benefits of standardizing methods of motor laterality assessment so that comparisons can be made between the populations sampled. By adopting such an approach, researchers should mutually benefit as motor laterality data could then be compared and subjected to meta-analysis.
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ISSN 1558-7878 ISBN Medium
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Notes Approved no
Call Number Equine Behaviour @ team @ S1558-7878(10)00038-9 Serial 5378
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Author Lorenz, K.
Title Die angeborenen Formen möglicher Erfahrung Type Journal Article
Year 1943 Publication Zeitschrift für Tierpsychologie Abbreviated Journal Zeitschrift für Tierpsychologie
Volume 5 Issue 2 Pages (down) 235-409
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Abstract Zusammenfassung Die vorliegende Abhandlung entspringt dem Aufgabenkreis einer jungen Arbeitsgemeinschaft zwischen Geisteswissenschaft und vergleichender Psychologie, die das wichtigste Programm des neugegründeten Philosophischen Institutes der Albertus-Universität in Königsberg darstellt. Sie ist aus der einen Abteilung dieser Anstalt, dem Institut für vergleichende Psychologie hervorgegangen. Obwohl sie eine ganze Reihe unveröffentlichter neuer Beobachtungen und Versuche enthält, stehen diese nicht genug im Mittelpunkte der Untersuchung, um eine Zusammenfassung von Ergebnissen in jener Form möglich zu machen, wie sie sonst in der induktiven Naturforschung üblich ist. Immerhin aber herrscht die induktive Denkweise in der vorliegenden Arbeit so stark vor, daß eine kurze Zusammenfassung angebracht erscbeint, wenn sie auch notgedrungen die Form einer kurzen Inhaltsangabe annehmea muß.
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Publisher John Wiley & Sons, Ltd Place of Publication Editor
Language Summary Language Original Title
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Series Volume Series Issue Edition
ISSN 0044-3573 ISBN Medium
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Notes doi: 10.1111/j.1439-0310.1943.tb00655.x Approved no
Call Number Equine Behaviour @ team @ Serial 6609
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Author Kruska, D.C.T.
Title Comparative quantitative investigations on brains of wild cavies (Cavia aperea) and guinea pigs (Cavia aperea f. porcellus). A contribution to size changes of CNS structures due to domestication Type Journal Article
Year 2014 Publication Mammalian Biology – Zeitschrift für Säugetierkunde Abbreviated Journal Mamm Biol
Volume 79 Issue 4 Pages (down) 230-239
Keywords Domestication; Allometry; Brain structure volumes; Brain-behavior correlation
Abstract Intraspecific allometric calculations of the brain to body size relation revealed distinct differences between 127 (67; 60) ancestral wild cavies and 82 (37; 45) guinea pigs, their domesticated relatives. The dependency of both measures from one another remained the same in both animal groups but the brains of guinea pigs were by 14.22% smaller at any net body weight. Consistent with results in other species the domestication of Cavia aperea is also characterized by a decrease of brain size. Fresh tissue sizes of the five brain parts medulla oblongata, cerebellum, mesencephalon, diencephalon and telencephalon were determined for 6 cavies and 6 guinea pigs by the serial section method. Additionally the sizes of 16 endbrain structures and those of the optic tract, the lateral geniculate body and the cochlear nucleus were measured. Different decrease values resulted for all these structures concomitant with domestication as was calculated from the amount of total brain size decrease and average relative structure values in the wild as well as the domesticated brain. The size decrease of the entire telencephalon (-13.7%) was within the range of the mean overall reduction as similarly was the case for the total neocortex (-10.7%) whereas the total allocortex (-20.9%) clearly was more strongly affected. The size decrease of the olfactory bulb (-41.9%) was extreme and clearly higher than found for the secondary olfactory structures (around -11%). The primary nuclei of other sensory systems (vision, audition) were decreased to less extent (lateral geniculate: -18.1%; cochlear nucleus: -12.6%). Mass decreases of pure white matter parts were nearly twice as high in contrast to associated grey matter parts (neocortex white versus grey matter; tractus opticus versus lateral geniculate body). The relatively great decrease values found for the limbic structures hippocampus (-26.9%) and schizocortex (-25.9%) are especially notable since they are in good conformity with domestication effects in other mammalian species. The findings of this study are discussed with regard to results of similar investigations on wild and domesticated gerbils (Meriones unguiculatus), the encephalization of the wild form, the special and species-specific mode and duration of domestication and in connection with certain behavioral changes as resulted from comparative investigations in ethology, socio-biology, endocrinology and general physiology.
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Series Volume Series Issue Edition
ISSN 1616-5047 ISBN Medium
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Notes Approved no
Call Number Admin @ knut @ Serial 6401
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Author Palme, R.
Title Non-invasive measurement of glucocorticoids: Advances and problems Type Journal Article
Year 2019 Publication Physiology & Behavior Abbreviated Journal Physiol. Behav.
Volume 199 Issue Pages (down) 229-243
Keywords Glucocorticoids; Non-invasive; Faecal cortisol/corticosterone metabolites; Immunoassays; Physiological/biological validation
Abstract Glucocorticoids (GCs; i.e. cortisol/corticosterone) are a central component of the stress response and thus their measurement is frequently used to evaluate the impact of stressful situations. Their metabolites from faeces of various animal species are more and more taken as a non-invasive aid to assess GC release and thus adrenocortical activity. The current literature review includes an extensive collection (1327 papers) and evaluation (see also Supplementary Tables) of the literature on faecal cortisol/corticosterone metabolite (FCM) analysis published to date. It aims at giving reference for researchers interested in implementing FCM analysis into their study or seeking to improve such methods by providing background knowledge on GC metabolism and excretion, conveying insights into methodological issues and stating caveats of FCM analysis and by highlighting prerequisites for and some examples of a successful application of such methods. Collecting faecal samples and analysing FCMs may appear simple and straightforward, but researchers have to select and apply methods correctly. They also need to be aware of the many pitfalls and potentially confounding factors and, last but not least, have to carefully interpret results. Applied properly, measurement of FCMs is a powerful non-invasive tool in a variety of research areas, such as (stress) biology, ethology, ecology, animal conservation and welfare, but also biomedicine.
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ISSN 0031-9384 ISBN Medium
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Notes Palme2019_attachment.pdf Approved no
Call Number Equine Behaviour @ team @ Serial 6517
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Author von Borstel, U.U.; Duncan, I.J.H.; Shoveller, A.K.; Merkies, K.; Keeling, L.J.; Millman, S.T.
Title Impact of riding in a coercively obtained Rollkur posture on welfare and fear of performance horses Type Journal Article
Year 2009 Publication Applied Animal Behaviour Science Abbreviated Journal Appl. Anim. Behav. Sci.
Volume 116 Issue 2-4 Pages (down) 228-236
Keywords Horse; Coercive riding; Welfare; Rollkur; Fear; Training
Abstract Rollkur, the usually coercively obtained hyperflexion of the horse's neck, is employed as a training method by some dressage riders; however, its use is controversial as it may cause discomfort and adversely affect the horse's welfare. The objectives of this study were to determine: (1) if horses showed differences in stress, discomfort and fear responses as measured by heart rate and behaviour when ridden in Rollkur (R) obtained by pressure on the reins compared to regular poll flexion (i.e. with the nose-line being at or just in front of the vertical; N), and (2) if they showed a preference between the two riding styles when given the choice. Fifteen riding horses were ridden 30 times through a Y-maze randomly alternating between sides. Riding through one arm of the Y-maze was always followed by a short round ridden in R, whereas riding through the other arm was followed by a short round ridden in N. Immediately after the conditioning phase, horses were again repeatedly ridden into the maze; however, riders left it to the horse to decide which arm of the maze to enter. During R, horses moved slower and showed more often behavioural signs of discomfort, such as tail-swishing, head-tossing or attempted bucks (P < 0.05), and 14 of the 15 horses chose significantly (P < 0.05) more often the maze-arm associated with N rather than R. Subsequently, eight of the horses were also subjected to two fear tests following a short ride in N as well as a ride in R. During R, horses tended to react stronger (P = 0.092) to the fear stimuli and to take longer (P = 0.087) to approach them. These findings indicate that a coercively obtained Rollkur position may be uncomfortable for horses and that it makes them more fearful and therefore potentially more dangerous to ride. Further studies need to assess horses' reaction to gradual training of Rollkur, as opposed to a coercively obtained hyperflexion, in order to decide whether the practice should be banned.
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ISSN 0168-1591 ISBN Medium
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Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5096
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Author Baragli, P.; Vitale, V.; Paoletti, E.; Sighieri, C.; Reddon, A.R.
Title Detour behaviour in horses (Equus caballus) Type Journal Article
Year 2011 Publication Journal of Ethology Abbreviated Journal J. Ethol.
Volume 29 Issue 2 Pages (down) 227-234
Keywords Detour behaviour; Equus caballus; Horses; Lateralization; Spatial reasoning
Abstract The objective of this study was to investigate the ability of horses (Equus caballus) to detour around symmetric and asymmetric obstacles. Ten female Italian saddle horses were each used in three detour tasks. In the first task, the ability to detour around a symmetrical obstacle was evaluated; in the second and third tasks subjects were required to perform a detour around an asymmetrical obstacle with two different degrees of asymmetry. The direction chosen to move around the obstacle and time required to make the detour were recorded. The results suggest that horses have the spatial abilities required to perform detour tasks with both symmetric and asymmetric obstacles. The strategy used to perform the task varied between subjects. For five horses, lateralized behaviour was observed when detouring the obstacle; this was consistently in one direction (three on the left and two on the right). For these horses, no evidence of spatial learning or reasoning was found. The other five horses did not solve this task in a lateralized manner, and a trend towards decreasing lateralization was observed as asymmetry, and hence task difficulty, increased. These non-lateralized horses may have higher spatial reasoning abilities.
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Publisher Springer Japan Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0289-0771 ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number Equine Behaviour @ team @ Serial 5686
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Author Irving-Pease, E.K.; Ryan, H.; Jamieson, A.; Dimopoulos, E.A.; Larson, G.; Frantz, L.A.F.
Title Paleogenomics of Animal Domestication Type Book Chapter
Year 2019 Publication Paleogenomics: Genome-Scale Analysis of Ancient DNA Abbreviated Journal
Volume Issue Pages (down) 225-272
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Abstract Starting with dogs, over 15,000 years ago, the domestication of animals has been central in the development of modern societies. Because of its importance for a range of disciplines – including archaeology, biology and the humanities – domestication has been studied extensively. This chapter reviews how the field of paleogenomics has revolutionised, and will continue to revolutionise, our understanding of animal domestication. We discuss how the recovery of ancient DNA from archaeological remains is allowing researchers to overcome inherent shortcomings arising from the analysis of modern DNA alone. In particular, we show how DNA, extracted from ancient substrates, has proven to be a crucial source of information to reconstruct the geographic and temporal origin of domestic species. We also discuss how ancient DNA is being used by geneticists and archaeologists to directly observe evolutionary changes linked to artificial and natural selection to generate a richer understanding of this fascinating process.
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Publisher Springer International Publishing Place of Publication Cham Editor Lindqvist, C.; Rajora, O.P.
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN 978-3-030-04753-5 Medium
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Notes Approved no
Call Number Equine Behaviour @ team @ Irving-Pease2019 Serial 6583
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Author Kuczaj, S. A; Makecha, R.; Trone, M.; Paulos, R. D; Ramos, J. A.
Title Role of Peers in Cultural Innovation and Cultural Transmission: Evidence from the Play of Dolphin Calves Type Journal Article
Year 2006 Publication International Journal of Comparative Psychology Abbreviated Journal Int. J. Comp. Psychol
Volume 19 Issue 2 Pages (down) 223-240
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Call Number Equine Behaviour @ team @ Serial 6526
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Author Byrne R.W.
Title The evolution of intelligence Type Book Chapter
Year 1994 Publication Behaviour and Evolution Abbreviated Journal
Volume Issue Pages (down) 223-265
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Publisher Cambridge University Press Place of Publication Cambridge,UK Editor P.J.B. Slater and T.R. Halliday
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Call Number Equine Behaviour @ team @ Serial 6566
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