Abstract: A modified spontaneous object recognition test was used to examine object recognition memory in the domestic pig. This test uses preference for a novel object over a previously encountered sample object as indicating recognition of the sample object, and no preference as indicating no recognition. Two factors hypothesized to affect object recognition are duration of exposure to the sample stimulus and delay interval before re-exposure. Both of these factors could be manipulated in a rotational object enrichment program for pigs. Reducing exposure time and increasing the delay interval before re-exposure should decrease object recognition and prolong novelty-induced object exploration. We exposed 5-week-old pigs to different sample objects in their home pens for 10 min and 2 days, respectively. We tested for object recognition memory at various delay intervals after initial exposure by placing littermate pairs in a test pen for 10 min and recording snout contact with a sample object and a completely novel object. At a 1-h delay, half the pairs were tested with the 2-day sample object; the other half received the 10-min sample object. At a 3-h delay, pairs were tested with the opposite sample object. Pairs were also tested with the 2-day sample at a 5-day delay and the 10-min sample at a 6-day delay. We predicted that pigs would show a preference for the novel versus the 2-day sample object at all three delays, but would only prefer the novel object over the 10-min sample object at the 1-h and 3-h delays. Pigs did not show novelty preference in the presence of the 10-min sample object at any delay. Novelty preference in the presence of the 2-day sample object occurred at the 3-h (P < 0.05) and 5-day delays (P < 0.001), but not the 1-h delay. The lack of novelty preference when pigs were tested with the 10-min sample object may have been due to failure to habituate to the sample object. Testing in a different location from the initial sample object exposure and retroactive interference from exposure to the 10-min sample object may have contributed to a temporary lack of novelty preference when pigs were tested with the 2-day sample object at the 1-h delay. The finding that pigs retained a memory for the 2-day sample object for at least 5 days suggests that restricting object exposure to less than 2 days may help to preserve the exploratory value of objects rotated among pens.

Abstract: This paper examines the rise of what is popularly called “natural horsemanship” (NH), as a definitive cultural change within the horse industry. Practitioners are often evangelical about their methods, portraying NH as a radical departure from traditional methods. In doing so, they create a clear demarcation from the practices and beliefs of the conventional horse-world. Only NH, advocates argue, properly understands the horse. Dissenters, however, contest the benefits to horses as well as the reliance in NH on disputed concepts of the natural. Advocates, furthermore, sought to rename technologies associated with riding while simultaneously condemning technologies used in conventional training (such as whips). These contested differences create boundaries and enact social inclusion and exclusion, which the paper explores. For horses, the impact of NH is ambiguous: Depending on practitioners, effects could be good or bad. However, for the people involved, NH presents a radical change-which they see as offering markedly better ways of relating to horses and a more inclusive social milieu.

Abstract: The first month of training of a young horse is suspected to be stressful, but the endocrine responses to initial training are unknown. Therefore in our study a total of 40 Quarter Horses (QH), all at the age of almost 2 years, were followed during the first 30 days of their training. During this time faecal samples were collected twice daily and faecal cortisol metabolites (FCM) were measured. Baseline values of FCM ranged between 1.3 and 20.1 (median: 6.7) ng/g faeces. No differences in FCM values between days of training were found. Mares showed the highest values. Significant diurnal variations were observed in mares (p=0.035) and stallions (p=0.003), but not in geldings (p=0.282). As in this study adrenocortical activity was not increased during initial training, horses seem to cope very well with this new situation. The results of our large-scale study provide basic physiological data about initial training. This gives additional input in an emotional debate about animal welfare aspects of first time handling and training of horses.
Abbreviations: 11,17-DOA = 11,17-dioxoandrostanes; EIA = Enyzme Immunoassay; FCM = faecal cortisol metabolites; GC = glucocorticoids; HPA-axis = hypothalamic-pituitary-adrenocortical-axis; QH = Quarter Horses
[Das Einreiten eines jungen Pferdes steht unter Verdacht belastend zu sein. Bisher gibt es aber keine Veröffentlichungen über endokrine Vorgänge während dieser Phase. Mit der vorliegenden Studie wurde überprüft, ob Pferde aufgrund physischer und psychischer Belastungen während des Trainings höhere Konzentrationen an Kortisolmetaboliten im Kot (FCM) aufweisen. Es wurden dazu 40 Quarter Horses im Alter von 2 Jahren während der ersten 30 Tage der Grundausbildung des Westernreitens beobachtet und ihre FCM Werte gemessen. Während dieser Zeitspanne wurden täglich morgens und abends Kotproben der Pferde genommen. Die Basalwerte der FCM Konzentration variierten zwischen 1,3 und 20,1 (Median: 6,7) ng/g Kot, wobei Stuten die höchsten Werte hatten. Signifikante Unterschiede während der einzelnen Trainingstage konnten nicht festgestellt werden. In der Tagesrhythmik wurden signifikante Unterschiede bei Stuten (p=0,035) und bei Hengsten (p=0,003), jedoch nicht bei Wallachen (p=0,282) ermittelt. In dieser Studie konnte keine erhöhte Aktivität der Nebennierenrinde im Verlauf der Grundausbildung eines Pferdes im Westernreitstil festgestellt werden. Das legt nahe, dass Pferde mit dieser neuen, zeitlich kurz andauernden Situationen gut zurechtkommen. Unsere Studie wurde an einer großen Anzahl von Tieren unter Feldbedingungen durchgeführt. Sie bietet daher eine gute Datenbasis über Belastungen während des Einreitens. Damit liefert sie einen zusätzlichen Beitrag zu einer mitunter emotional geführten Debatte über tierschutzrelevante Aspekte bei der Grundausbildung von Pferden.]

Abstract: Management of grazed grasslands for production and/or conservation objectives requires a thorough understanding of the choices of feeding sites by herbivores, and of the biological processes involved. Most models of the feeding strategies of herbivores are based on the principle that optimising the intake of energy (or some nutrient) is the primary goal of foragers but other selective forces, such as parasitism, could be important. Gastrointestinal parasites (including cyathostome nematodes) have powerful effects on the fitness of herbivores and may act as a major selection pressure favouring host behaviour that reduces the risk of encountering parasites. Among large herbivores, horses have perhaps the most marked tendency to select particular feeding sites within grasslands. We test here: (1) whether horses select feeding patches with relatively low parasite densities and (2) if their choice is affected by their parasite load. We used 10 two-year old saddle-horses and three periods. In the first period, the horses were under natural parasitism which varied strongly among individuals; in the second period they were all dewormed, and in the third, a sub-set of the horses was experimentally infected with cyathostome larvae. Ninety-eight percent of the infective larvae in the pasture were found <1 m from faeces. The main determinant of the choice of feeding patch by horses was the availability of patches of different parasite risk and grass height. Controlling for availability, the horses used tall grasses (>16 cm) less than expected, whether the grass was contaminated or not, and they selected for short patches >1 m from faeces, where the risk of encountering parasites was low. These results suggest that selection of feeding sites by horses is driven by an interaction between their nutritional and anti-parasite strategies: the horses avoid the patches of tall grass which are generally of low quality and areas contaminated by parasite larvae which leads them to prefer the patches of short grass far from faeces. The parasite status of the horses at the time of the experiment had no effect on their feeding choices. However, before concluding that the challenge by cyathostomes has no effect on the selection of feeding sites in horses, it will be necessary to test whether the history of parasitism of the individuals, rather than the current status, is important.

Abstract: INTRODUCTION: While fibrin sealant (FS) and equine collagen (EC) have been used as scaffold materials in experimental spinal cord injury (SCI), questions concerning neurocompatibility still remain. In this study, we assessed potential adverse effects, as well as functional and histological impact of FS and EC in subtotal hemisection of the thoracic spinal cord (SC) in rats. METHODS: 124 male rats were randomly assigned to four main groups (n=31): Sham (SH), Lesion only (L), fibrin sealant (GFS) and equine collagen group (GEC). SH animals received laminectomy only; all other animals underwent subtotal lateral hemisection at T9. Treatment consisted of application of FS or EC into the lesion gap in GFS and GEC, which was left empty in L. GFS, GEC, L and SH were each further divided into 4 subgroups: One subgroup, consisting of 10 rats was subjected to behavioural and reflex testing before surgery and followed up on days 1,7, 14, 21, 28 post op and then sacrificed. Haemalaun or cresyl violet (CV) was used to identify neutrophils in parasagittal cord sections which were obtained on day 1 (n=7). Sections stained for quantification of microglia/macrophages using ED-1 on day 3 (n=7), day 7 (n=7) and day 28 (n=7 out of 10). Additionally, neural filament (NF) staining was chosen to detect axonal regeneration and the length of ingrowth into FS and EC, Luxol blue for myelination, Von Willebrand factor for vascularisation, and glial fibrillary acidic protein (GFAP) staining for detection of astrocytes in glial scars on day 28. RESULTS: No adverse effects were observed in the treatment groups. Compared to L, GFS and GEC performed significantly better in the Basso, Beattie, Bresnahan (BBB) score and hopping responses. Proprioceptive placing was markedly improved in FS and EC compared to L. Axonal regrowth was found in GFS and GEC--the regrowth in the GFS was accompanied by myelination and vascularisation. Glial scarring occurred in all groups. Discussion Both biomatrices improved functional recovery compared to L and no adverse effects were perceived.