Records |
Author |
Li, C.; Jiang, Z.; Tang, S.; Zeng, Y. |
Title |
Influence of enclosure size and animal density on fecal cortisol concentration and aggression in Pere David's deer stags |
Type |
Journal Article |
Year |
2007 |
Publication |
General and Comparative Endocrinology |
Abbreviated Journal |
Gen Comp Endocrinol |
Volume |
151 |
Issue |
2 |
Pages |
202-209 |
Keywords |
*Aggression; Animals; *Deer; *Environment; Feces/*chemistry; Handling (Psychology); Housing, Animal; Hydrocortisone/*analysis; Male; Population Density |
Abstract |
We investigated the impact of enclosure size and animal density on behavior and adrenocortical secretion in Pere David's deer in Dafeng Nature Reserve, China. From February 15 to April 16 in 2004, we conducted two experiments. First, we studied maintenance behavior and conflict behavior of Pere David's deer stags in a large enclosure (200 ha) with low animal density (0.66 deer/ha) and a small display pen (0.75 ha) with high animal density (25.33 deer/ha). The maintenance behavior we recorded included standing, locomotion, foraging and rest. During the behavioral observations, we collected fresh voided fecal samples from the stags periodically, and analyzed the fecal cortisol concentrations in those samples using radioimmunoassay technique. Second, we monitored the fecal cortisol concentrations of one group of stags (12 deer lived in an enclosure of 100 ha) before and after transferred into a small pen (0.5 ha). We found that in the first experiment: (1) there were significant differences in standing and rest whereas no significant differences of locomotion and foraging between the free-ranging group and the display group; (2) frequency of conflict behavior in the display group was significantly higher than those in the free-ranging group; and (3) fecal cortisol concentration of the display group (326.17+/-16.98 ng/g dry feces) was significantly higher than that of the free-ranging group (268.98+/-15.21 ng/g dry feces). In the second experiment, there was no significant difference of the fecal cortisol concentrations among sampling days, but the mean fecal cortisol concentration of the day after transferring (337.46+/-17.88 ng/g dry feces) was significantly higher than that of the day before transferring (248.44+/-7.99 ng/g dry feces). Comparison with published findings, our results indicated that enclosure size and animal density affect not only behaviors, but also adrenocortical secretion in Pere David's deer. Small living space with high animal density may impose physiological stress to captive Pere David's deer. Moreover, long-term physiological stress and increase of conflict behavior may subsequently affect survival and reproduction of the deer. |
Address |
Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China |
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English |
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0016-6480 |
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PMID:17324429 |
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no |
Call Number |
Equine Behaviour @ team @ |
Serial |
5475 |
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Author |
Carlsson, H.-E.; Lyberg, K.; Royo, F.; Hau, J. |
Title |
Quantification of stress sensitive markers in single fecal samples do not accurately predict excretion of these in the pig |
Type |
Journal Article |
Year |
2007 |
Publication |
Research in Veterinary Science |
Abbreviated Journal |
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Volume |
82 |
Issue |
3 |
Pages |
423-428 |
Keywords |
Cortisol; Immunoglobulin A; Stress; Pigs; Feces; Animal welfare |
Abstract |
All feces produced during 24 h were collected from five pigs and cortisol and immunoreactive cortisol metabolites (CICM), and IgA were quantified. Within pigs, the concentrations of CICM and IgA varied extensively between random samples obtained from a single fecal dropping, and deviated in most cases significantly from the true concentration measured in total fecal output (CV 6.7–130%). The CICM and IgA contents varied considerably (CV 8.1–114%) within and between individual fecal droppings from the same pig compared to the total fecal excretion. In conclusion, single random samples could not be used to reliably quantify the total fecal concentration or excretion of CICM or IgA in pigs. Analyses of all feces collected during shorter periods than 24 h did not provide an accurate estimate of the daily excretion of CICM. Thus, the concentration of stress sensitive molecules in random single fecal samples as an indicator of animal welfare should be interpreted with prudence. |
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0034-5288 |
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Equine Behaviour @ team @ |
Serial |
5853 |
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Author |
Vetvik, H.; Grewal, H.M.S.; Haugen, I.L.; Åhrén, C.; Haneberg, B. |
Title |
Mucosal antibodies can be measured in air-dried samples of saliva and feces |
Type |
Journal Article |
Year |
1998 |
Publication |
Journal of Immunological Methods |
Abbreviated Journal |
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Volume |
215 |
Issue |
1–2 |
Pages |
163-172 |
Keywords |
Saliva; Feces; IgA; IgG; Air-drying |
Abstract |
IgA antibodies reflecting airways or intestinal mucosal immune responses can be found in saliva and feces, respectively, and IgG antibodies reflecting serum antibodies can be found in saliva. In this study, antibodies were detected in samples of saliva and feces which had been air-dried at room temperature (+20°C) or +37°C, and stored at these temperatures for up to 6 months. In saliva the antibody levels increased, while the antibodies in feces decreased upon storage. The individual IgA antibody concentrations which were adjusted by using the ratios of specific IgA/total IgA were relatively stable in both saliva and feces, and correlated with corresponding antibody levels in samples which had been stored at -20°C. The results indicate that air-dried saliva and feces can be used for semiquantitative measurements of mucosal antibodies, even after prolonged storage at high temperatures and lack of refrigeration. |
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0022-1759 |
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Equine Behaviour @ team @ |
Serial |
5996 |
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Author |
Taberlet, P.; Waits, L.P.; Luikart, G. |
Title |
Noninvasive genetic sampling: look before you leap |
Type |
Journal Article |
Year |
1999 |
Publication |
Trends in Ecology & Evolution |
Abbreviated Journal |
Trends Ecol. Evol |
Volume |
14 |
Issue |
8 |
Pages |
323-327 |
Keywords |
Hairs; Feces; Feathers; Allelic dropout; Individual identification; Conservation genetics; Behavioural ecology; Pilot study; Microsatellites; Probability of identity |
Abstract |
Noninvasive sampling allows genetic studies of free-ranging animals without the need to capture or even observe them, and thus allows questions to be addressed that cannot be answered using conventional methods. Initially, this sampling strategy promised to exploit fully the existing DNA-based technology for studies in ethology, conservation biology and population genetics. However, recent work now indicates the need for a more cautious approach, which includes quantifying the genotyping error rate. Despite this, many of the difficulties of noninvasive sampling will probably be overcome with improved methodology. |
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ISSN |
0169-5347 |
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no |
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Equine Behaviour @ team @ |
Serial |
6573 |
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Author |
Alexander, F. |
Title |
The effect of some anti-diarrhoeal drugs on intestinal transit and faecal excretion of water and electrolytes in the horse |
Type |
Journal Article |
Year |
1978 |
Publication |
Equine veterinary journal |
Abbreviated Journal |
Equine Vet J |
Volume |
10 |
Issue |
4 |
Pages |
229-234 |
Keywords |
Animals; Antidiarrheals/*pharmacology; Atropine/pharmacology; Electrolytes/*analysis/urine; Feces/*analysis; Gastrointestinal Motility/*drug effects; Horses/*metabolism/physiology; Loperamide/pharmacology; Male; Meperidine/pharmacology; Morphine/pharmacology; Opium/pharmacology; Water/*analysis |
Abstract |
The effect of morphine, Tinct. opii, loperamide, pethidine and atropine on intestinal transit and the faecal and urinary excretion of water and electrolytes was studied in ponies. The rate of passage of a particulate marker was slowed by morphine, hastened then slowed by loperamide and Tinct. opii, and hastened by atropine. The liquid marker was slowed by Tinct. opii and hastened then slowed by the other drugs. Only loperamide decreased the faecal sodium excretion. This drug also decreased faecal water and weight; it appeared worthy of clinical trial in diarrhoea. Tinct. opii decreased by morphine, pethidine and atropine increased faecal water. |
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English |
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0425-1644 |
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Notes |
PMID:738263 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
110 |
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Author |
Alexander, F. |
Title |
The effect of diuretics on the faecal excretion of water and electrolytes in horses |
Type |
Journal Article |
Year |
1977 |
Publication |
British journal of pharmacology |
Abbreviated Journal |
Br J Pharmacol |
Volume |
60 |
Issue |
4 |
Pages |
589-593 |
Keywords |
Animals; Body Water/*metabolism; Chlorides/metabolism; Electrolytes/*metabolism; Feces/*analysis; Furosemide/*pharmacology; Horses; Hydrochlorothiazide/*pharmacology; Male; Potassium/metabolism; Sodium/metabolism; Time Factors |
Abstract |
1. The effect on plasma, urinary and faecal electrolytes of frusemide and hydrochlorthiazide was measured in ponies, mean weight 180 kg. 2. The rapid loss in urine of large quantities of sodium had only a small effect on plasma sodium concentration. 3. Faecal sodium excretion was increased substantially after the administration of frusemide. 4. Frusemide increased faecal potassium during the 48 h following administration and faecal water in the 24/48 h period. It also produced a hypopotassaemia. 5. Hydrochlorthiazide increased faecal chloride during the 24 h after administration. 6. Frusemide increased the intestinal transit time of both liquid (polyethylene glycol) and particulate (Cr2O3) markers. |
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ISSN |
0007-1188 |
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Notes |
PMID:907872 |
Approved |
no |
Call Number |
refbase @ user @ |
Serial |
111 |
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Author |
Alexander, F.; Davies, M.E. |
Title |
Studies on vitamin B12 in the horse |
Type |
Journal Article |
Year |
1969 |
Publication |
The British veterinary journal |
Abbreviated Journal |
Br. Vet. J. |
Volume |
125 |
Issue |
4 |
Pages |
169-176 |
Keywords |
Animals; Biological Assay; Cattle; Chickens; Feces/analysis; Horses/*metabolism; Humans; Lactobacillus/metabolism; Rabbits; Rats; Sheep; Urine/analysis; Vitamin B 12/*analysis |
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English |
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ISSN |
0007-1935 |
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PMID:5814055 |
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no |
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refbase @ user @ |
Serial |
116 |
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Author |
Turner, J.W.J.; Liu, I.K.; Kirkpatrick, J.F. |
Title |
Remotely delivered immunocontraception in free-roaming feral burros (Equus asinus) |
Type |
Journal Article |
Year |
1996 |
Publication |
Journal of reproduction and fertility |
Abbreviated Journal |
J Reprod Fertil |
Volume |
107 |
Issue |
1 |
Pages |
31-35 |
Keywords |
Animals; *Animals, Wild; Contraception, Immunologic/methods/*veterinary; *Equidae; Feces/chemistry; Female; Pregnancy; Pregnancy Tests; Swine; Zona Pellucida/immunology |
Abstract |
Regulation of local overpopulations of free-roaming feral equids is in demand worldwide for ecological balance and habitat preservation. Contraceptive vaccines have proven effective in feral horses, which breed seasonally, but no data are available for equids such as the burro, which is reproductively active all year round. In the present study, 27 individually identified female feral burros (Equus asinus) roaming free in Virgin Islands National Park (St John, US Virgin Islands; Lesser Antilles) were remotely treated with pig zonae pellucidae (PZP) vaccine. Between January and May, 16 burros were darted with a 1 ml emulsion of PZP plus Freund's adjuvant. Ten to twelve months later each treated burro was given a single booster injection of PZP plus adjuvant to maintain contraception through a second year. Eleven adult untreated jennies served as controls. Beginning one year after initial vaccination, these burros were monitored for pregnancy and foal production. Collection of data to determine treatment effect was not begun until 12 months after initial treatment to ensure that pregnancies existing before vaccination were not included. Pregnancy was assessed using previously validated methods for steroid metabolite measurement in fresh faecal samples. None of the PZP-treated burros produced foals between 0 and 12 months after the last inoculation. One PZP-treated burro tested positive for pregnancy at 10 months after the final inoculation. During this same period, six of 11 untreated burros tested pregnancy-positive, and four were observed with foals. There was no difference in pregnancy rates among treated, control and randomly sampled jennies between 12 and 24 months after the last inoculation. The results demonstrate that, in free-roaming feral burros that are reproductively active all year round: (1) burros can be accessed for remotely delivered PZP vaccination; (2) PZP contraception is effective; (3) PZP contraception is reversible; and (4) pregnancy can be reliably detected by faecal steroid analysis. |
Address |
Department of Physiology and Molecular Medicine, Medical College of Ohio, Toledo 43699, USA |
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0022-4251 |
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PMID:8699431 |
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refbase @ user @ |
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144 |
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Author |
Husted, L.; Andersen, M.S.; Borggaard, O.K.; Houe, H.; Olsen, S.N. |
Title |
Risk factors for faecal sand excretion in Icelandic horses |
Type |
Journal Article |
Year |
2005 |
Publication |
Equine Veterinary Journal |
Abbreviated Journal |
Equine Vet J |
Volume |
37 |
Issue |
4 |
Pages |
351-355 |
Keywords |
Animal Feed; Animal Husbandry/methods; Animals; Denmark; Feces/*chemistry; Female; Gastrointestinal Diseases/epidemiology/etiology/prevention & control/veterinary; Horse Diseases/epidemiology/etiology/prevention & control; Horses/*metabolism; Logistic Models; Male; Pilot Projects; *Poaceae/growth & development; Risk Factors; Silicon Dioxide/administration & dosage/adverse effects/*analysis; Soil/*analysis |
Abstract |
REASONS FOR PERFORMING STUDY: Sandy soil is often mentioned as a risk factor in the development of sand-related gastrointestinal disease (SGID) in the horse. There are other variables, but few studies confirm any of these. OBJECTIVE: To investigate soil type, pasture quality, feeding practice in the paddock, age, sex and body condition score as risk factors for sand intake in the horse. METHODS: Faeces were collected from 211 Icelandic horses on 19 different studs in Denmark together with soil samples and other potential risk factors. Sand content in faeces determined by a sand sedimentation test was interpreted as evidence of sand intake. Soil types were identified by soil analysis and significance of the data was tested using logistic analysis. RESULTS: Of horses included in the study, 56.4% showed sand in the faeces and 5.7% had more than 5 mm sand as quantified by the rectal sleeve sedimentation test. Soil type had no significant effect when tested as main effect, but there was interaction between soil type and pasture quality. Significant interactions were also found between paddock feeding practice and pasture quality. CONCLUSION: To evaluate the risk of sand intake it is important to consider 3 variables: soil type, pasture quality and feeding practice. Pasture quality was identified as a risk factor of both short and long grass in combination with sandy soil, while clay soil had the lowest risk in these combinations. Feeding practice in the paddock revealed feeding directly on the ground to be a risk factor when there was short (1-5 cm) or no grass. Also, no feeding outdoors increased the risk on pastures with short grass, while this had no effect in paddocks with no grass. More than 50% of all horses investigated in this study had sand in the faeces. POTENTIAL RELEVANCE: The identification of risk factors is an important step towards prevention of SGID. Further research is necessary to determine why some horses exhibit more than 5 mm sand in the sedimentation test and whether this is correlated with geophagic behaviour. |
Address |
Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlaegevej 88, DK-1870 Frederiksberg, Denmark |
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0425-1644 |
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PMID:16028626 |
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no |
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Serial |
1888 |
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Author |
Milinovich, G.J.; Trott, D.J.; Burrell, P.C.; van Eps, A.W.; Thoefner, M.B.; Blackall, L.L.; Al Jassim, R.A.M.; Morton, J.M.; Pollitt, C.C. |
Title |
Changes in equine hindgut bacterial populations during oligofructose-induced laminitis |
Type |
Journal Article |
Year |
2006 |
Publication |
Environmental Microbiology |
Abbreviated Journal |
Environ Microbiol |
Volume |
8 |
Issue |
5 |
Pages |
885-898 |
Keywords |
Animal Feed; Animals; Bacteria/classification/*isolation & purification; DNA, Bacterial/analysis; Disease Models, Animal; Feces/microbiology; Foot Diseases/etiology/microbiology/*veterinary; Horse Diseases/*etiology/metabolism/microbiology; Horses; In Situ Hybridization, Fluorescence; Intestines/*microbiology; Oligosaccharides/*administration & dosage/*metabolism; Phylogeny; Polymerase Chain Reaction; RNA, Bacterial/analysis; RNA, Ribosomal, 16S/analysis |
Abstract |
In the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. The objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. Laminitis was induced in five horses by administration of oligofructose. Faecal specimens were collected at 8 h intervals from 72 h before to 72 h after the administration of oligofructose. Hindgut microbiota able to utilize oligofructose were enumerated throughout the course of the experiment using habitat-simulating medium. Isolates were collected and representatives identified by 16S rRNA gene sequencing. The majority of these isolates collected belonged to the genus Streptococcus, 91% of which were identified as being most closely related to Streptococcus infantarius ssp. coli. Furthermore, S. infantarius ssp. coli was the predominant oligofructose-utilizing organism isolated before the onset of lameness. Fluorescence in situ hybridization probes developed to specifically target the isolated Streptococcus spp. demonstrated marked population increases between 8 and 16 h post oligofructose administration. This was followed by a rapid population decline which corresponded with a sharp decline in faecal pH and subsequently lameness at 24-32 h post oligofructose administration. This research suggests that streptococci within the Streptococcus bovis/equinus complex may be involved in the series of events which precede the onset of laminitis in the horse. |
Address |
Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Queensland, Australia. g.milinovich@uq.edu.au |
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1462-2912 |
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PMID:16623745 |
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Equine Behaviour @ team @ |
Serial |
2625 |
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