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Author Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B.
Title Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces Type Journal Article
Year 2004 Publication Parasitology Abbreviated Journal Parasitology
Volume 129 Issue Pt 6 Pages 733-739
Keywords Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics
Abstract Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1820 ISBN Medium
Area Expedition Conference
Notes PMID:15648696 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2631
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Author Dargatz, D.A.; Traub-Dargatz, J.L.
Title Multidrug-resistant Salmonella and nosocomial infections Type Journal Article
Year 2004 Publication The Veterinary Clinics of North America. Equine Practice Abbreviated Journal Vet Clin North Am Equine Pract
Volume 20 Issue 3 Pages 587-600
Keywords Animals; Anti-Bacterial Agents/*pharmacology; Cross Infection/prevention & control/*veterinary; Disease Outbreaks/prevention & control/veterinary; Drug Resistance, Bacterial; *Drug Resistance, Multiple, Bacterial; Horse Diseases/*drug therapy/transmission; Horses; Infection Control/methods; Microbial Sensitivity Tests/veterinary; Salmonella/*drug effects; Salmonella Infections, Animal/*drug therapy/transmission
Abstract Nosocomial infections are a serious threat to optimum patient care. In addition, nosocomial infections can have far-reaching consequences for the hospital personnel and the financial aspects of the hospital. Nosocomial infections with Salmonella spp have been described among hospitalized equine populations more frequently than any other agent. Salmonella spp associated with hospitalized equids often possess more antimicrobial resistance determinants than do Salmonella spp isolated from healthy horses in the general population. There is little evidence to suggest that resistant salmonellae are more virulent than nonresistant forms. MDR forms of Salmonella complicate the selection of appropriate antimicrobials when they are indicated, however. Furthermore, the use of some antimicrobials may apply selection pressure toward enhanced ability of MDR Salmonella to colonize equine patients. Further research should help to elucidate the risky uses of antimicrobials in the hospital setting and define the role of disinfectants and treatments such as NSAIDs in the ecology of MDR forms of nosocomial infections, including Salmonella. In the meantime, thoughtful selection of when and how to use antimicrobials in equine patients, together with deliberate selection of which antimicrobials to use based on monitoring data and other factors, such as safety and spectrum, is advised.
Address Animal and Plant Health Inspection Service, Veterinary Services, Centers for Epidemiology and Animal Health, United States Department of Agriculture, 2150 Centre Avenue, Building MS 2E7, Fort Collins, CO 80521, USA. david.a.dargatz@aphis.usda.gov
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0749-0739 ISBN Medium
Area Expedition Conference
Notes PMID:15519820 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2632
Permanent link to this record
 

 
Author Giangaspero, A.; Traversa, D.; Otranto, D.
Title [Ecology of Thelazia spp. in cattle and their vectors in Italy] Type Journal Article
Year 2004 Publication Parassitologia Abbreviated Journal Parassitologia
Volume 46 Issue 1-2 Pages 257-259
Keywords Animals; Cattle/parasitology; Cattle Diseases/epidemiology/*parasitology/transmission; Disease Transmission, Horizontal; Dog Diseases/epidemiology/parasitology/transmission; Dogs/parasitology; Ecosystem; Eye Infections, Parasitic/epidemiology/transmission/*veterinary; Horse Diseases/epidemiology/parasitology/transmission; Horses/parasitology; Humans; Insect Vectors/*parasitology; Italy/epidemiology; Muscidae/*parasitology; Species Specificity; Spirurida Infections/epidemiology/transmission/*veterinary; Thelazioidea/classification/*isolation & purification
Abstract The genus Thelazia (Spirurida, Thelaziidae) includes a cosmopolitan group of eyeworm spirurids responsible for ocular infections in domestic and wild animals and transmitted by different species of muscids. Bovine thelaziosis is caused by Thelazia rhodesi Desmarest 1828, Thelazia gulosa Railliet & Henry 1910, and Thelazia skrjabini Erschow 1928, which occur in many countries; T. gulosa and T. skrjabini have been reported mainly in the New World, while T. rhodesi is particularly common in the Old World. In Italy, T. rhodesi was reported in southern regions a long time ago and, recently, T. gulosa and T. skrjabini have been identified in autochthonous cattle first in Apulia and then in Sardinia. Thirteen species of Musca are listed as intermediate hosts of eyeworms, but only Musca autumnalis and Musca larvipara have been demonstrated to act as vectors of Thelazia in the ex-URSS, North America, ex-Czechoslovakia and more recently in Sweden. In Italy, after the reports of T. gulosa and T. skrjabini in southern regions, the intermediate hosts of bovine eyeworms were initially only suspected as the predominant secretophagous Muscidae collected from the periocular region of cattle with thelaziosis were the face flies, M. autumnalis and M. larvipara, followed by Musca osiris, Musca tempestiva and Musca domestica. The well-known constraints in the identification of immature eyeworms to species by fly dissection and also the time-consuming techniques used constitute important obstacles to epidemiological field studies (i.e. vector identification and/or role, prevalence and pattern of infection in flies, etc.). Molecular studies have recently permitted to further investigations into this area. A PCR-RFLP analysis of the ribosomal ITS-1 sequence was developed to differentiate the 3 species of Thelazia (i.e. T. gulosa, T. rhodesi and T. skrjabini) found in Italy, then a molecular epidemiological survey has recently been carried out in field conditions throughout five seasons of fly activity and has identified the role of M. autumnalis, M. larvipara, M. osiris and M. domestica as vectors of T. gulosa and of M. autumnalis and M. larvipara of T. rhodesi. Moreover, M. osiris was described, for the first time, to act as a vector of T. gulosa and M. larvipara of T. gulosa and T. rhodesi. The mean prevalence in the fly population examined was found to be 2.86%. The molecular techniques have opened new perspectives for further research on the ecology and epidemiology not only of Thelazia in cattle but also of other autochthonous species of Thelazia which have been also recorded in Italy, such as Thelazia callipaeda, which is responsible for human and canid ocular infection and Thelazia lacrymalis, the horse eyeworm whose epidemiological molecular studies are in progress.
Address Dipartimento PR.I.M.E., Universita degli Studi di Foggia
Corporate Author Thesis
Publisher Place of Publication Editor
Language Italian Summary Language Original Title Ecologia di Thelazia spp. e dei vettori in Italia
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0048-2951 ISBN Medium
Area Expedition Conference
Notes PMID:15305729 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2633
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Author Nelson, D.M.; Gardner, I.A.; Chiles, R.F.; Balasuriya, U.B.; Eldridge, B.F.; Scott, T.W.; Reisen, W.K.; James Maclachlan, N.
Title Prevalence of antibodies against Saint Louis encephalitis and Jamestown Canyon viruses in California horses Type Journal Article
Year 2004 Publication Comparative Immunology, Microbiology and Infectious Diseases Abbreviated Journal Comp Immunol Microbiol Infect Dis
Volume 27 Issue 3 Pages 209-215
Keywords Animals; Antibodies, Viral/*blood; California/epidemiology; Encephalitis Virus, California/*immunology/isolation & purification; Encephalitis Virus, St. Louis/*immunology/isolation & purification; Encephalitis, St. Louis/epidemiology/immunology/*veterinary/virology; Female; Horse Diseases/epidemiology/immunology/*virology; Horses; Logistic Models; Male; Neutralization Tests/veterinary; Polyomavirus Infections/epidemiology/immunology/*veterinary/virology; Questionnaires; Seroepidemiologic Studies; Tumor Virus Infections/epidemiology/immunology/*veterinary/virology
Abstract Jamestown Canyon (JC) and Saint Louis encephalitis (SLE) viruses are mosquito-transmitted viruses that have long been present in California. The objective of this study was to determine the seroprevalence of these two viruses in horses prior to the introduction of West Nile (WN) virus. Approximately 15% of serum samples collected in 1998 from 425 horses on 44 equine operations horses throughout California had serum antibodies to JC virus, whereas antibodies were not detected to SLE virus. The results indicate that horses in California were commonly infected prior to 1998 with mosquito-transmitted Bunyaviruses that are identical or closely related to JC virus, but not with SLE virus. The different seroprevalence of SLE and JC viruses in horses likely reflects the unique ecology of each virus, and it is predicted that WN virus will have a wider distribution in California than closely related SLE virus.
Address Animal and Plant Health Inspection Service, Veterinary Services, U.S. Department of Agriculture, California and Nevada Area Office, 9850 Micron Avenue, Suite E, Sacramento, CA 95827, USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0147-9571 ISBN Medium
Area Expedition Conference
Notes PMID:15001316 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2637
Permanent link to this record
 

 
Author Komar, N.
Title West Nile virus: epidemiology and ecology in North America Type Journal Article
Year 2003 Publication Advances in Virus Research Abbreviated Journal Adv Virus Res
Volume 61 Issue Pages 185-234
Keywords Animals; Bird Diseases/virology; Birds/virology; Culex/virology; Disease Reservoirs; Ecosystem; Epidemiology, Molecular; Horse Diseases/virology; Horses/virology; Humans; Insect Vectors; North America/epidemiology; Risk Factors; West Nile Fever/*epidemiology/transmission/veterinary; West Nile virus/genetics
Abstract
Address Centers for Disease Control and Prevention, Division of Vector-Borne Infectious Diseases, Fort Collins, Colorado 80522, USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0065-3527 ISBN Medium
Area Expedition Conference
Notes PMID:14714433 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2638
Permanent link to this record
 

 
Author Valero, N.
Title West Nile virus: a new challenge? Type Journal Article
Year 2003 Publication Investigacion Clinica Abbreviated Journal Invest Clin
Volume 44 Issue 3 Pages 175-177
Keywords Animal Migration; Animals; Bird Diseases/virology; Birds; Caribbean Region; Central America; Culex/virology; Horse Diseases/virology; Horses; Humans; Insect Vectors/virology; North America/epidemiology; South America; West Nile Fever/*epidemiology/transmission/veterinary; West Nile virus/*physiology
Abstract West Nile Virus (WNV), a member of the family Flaviviridae, was first isolated in 1937. Since the original isolation of the WNV outbreaks have occurred with increase in frequency of cases in humans and horses, apparent increase in severe human disease and high avian death rates. In 1999, 2000 and 2002 outbreaks of the WNV encephalitis were reported in horses, birds and humans from New York and Canada. Ornithophilic mosquitoes are the principal vectors of the WNV and birds of several species chiefly migrants appear to be the major introductory or amplifying host. The pattern of outbreaks in the old and new world suggests that viremic migratory birds may also contribute to movement of the virus. If so, Central America, Caribbean Islands and countries of South America including Venezuela, are in potential risk for suffering a severe outbreak for WNV, since several species of birds have populations that pass trough New York and cross the western north Atlantic or Caribbean Sea. It is important the knowledge of the ecology of WNV as well of the efficacy of control efforts in order to minimize the public health impact in these countries, where all population is susceptible to this infection.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Spanish Summary Language Original Title Virus del Nilo Occidental: Un nuevo reto?
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0535-5133 ISBN Medium
Area Expedition Conference
Notes PMID:14552056 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2640
Permanent link to this record
 

 
Author Hall, R.A.; Broom, A.K.; Smith, D.W.; Mackenzie, J.S.
Title The ecology and epidemiology of Kunjin virus Type Journal Article
Year 2002 Publication Current Topics in Microbiology and Immunology Abbreviated Journal Curr Top Microbiol Immunol
Volume 267 Issue Pages 253-269
Keywords Animals; Culicidae/virology; Ecosystem; Horse Diseases/etiology; Horses; Humans; Insect Vectors; Population Surveillance; West Nile Fever/*epidemiology/*etiology/veterinary; West Nile virus/classification/genetics/immunology/*isolation & purification
Abstract
Address Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia, Queensland 4072, Australia
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0070-217X ISBN Medium
Area Expedition Conference
Notes PMID:12082993 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2642
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Author Marfin, A.A.; Petersen, L.R.; Eidson, M.; Miller, J.; Hadler, J.; Farello, C.; Werner, B.; Campbell, G.L.; Layton, M.; Smith, P.; Bresnitz, E.; Cartter, M.; Scaletta, J.; Obiri, G.; Bunning, M.; Craven, R.C.; Roehrig, J.T.; Julian, K.G.; Hinten, S.R.; Gubler, D.J.
Title Widespread West Nile virus activity, eastern United States, 2000 Type Journal Article
Year 2001 Publication Emerging Infectious Diseases Abbreviated Journal Emerg Infect Dis
Volume 7 Issue 4 Pages 730-735
Keywords Animals; Bird Diseases/epidemiology/virology; Culicidae/virology; *Disease Outbreaks; Ecology; Horse Diseases/epidemiology/virology; Horses; Humans; Population Surveillance; Songbirds/virology; United States/epidemiology; West Nile Fever/*epidemiology/veterinary/virology; *West Nile virus
Abstract In 1999, the U.S. West Nile (WN) virus epidemic was preceded by widespread reports of avian deaths. In 2000, ArboNET, a cooperative WN virus surveillance system, was implemented to monitor the sentinel epizootic that precedes human infection. This report summarizes 2000 surveillance data, documents widespread virus activity in 2000, and demonstrates the utility of monitoring virus activity in animals to identify human risk for infection.
Address Division of Vector-Borne Infections Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Fort Collins, CO 80522, USA. aam@cdc.gov
Corporate Author ArboNET Cooperative Surveillance Group Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 1080-6040 ISBN Medium
Area Expedition Conference
Notes PMID:11585539 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2646
Permanent link to this record
 

 
Author Barwick, R.S.; Mohammed, H.O.; McDonough, P.L.; White, M.E.
Title Epidemiologic features of equine Leptospira interrogans of human significance Type Journal Article
Year 1998 Publication Preventive Veterinary Medicine Abbreviated Journal Prev Vet Med
Volume 36 Issue 2 Pages 153-165
Keywords Animals; Horse Diseases/epidemiology/*microbiology; Horses/microbiology; Humans; *Leptospira interrogans; New York/epidemiology; Weil Disease/epidemiology/microbiology/*veterinary; Zoonoses/epidemiology/*microbiology
Abstract Leptospirosis is a zoonotic bacterial disease caused by Leptospira interrogans. There is a serologic evidence that horses are exposed to L. interrogans and, as a shedder of these organisms, can be a threat to humans. We examined risk factors associated with the risk of testing seropositive to three L. interrogans serovars (L. icterohaemorrhagiae, L. grippotyphosa, and L. canicola) in the horses of New York State, in order to understand the epidemiology of the disease and suggest strategies to control and prevent equine leptospirosis. To carry out this study, blood samples were collected from a random sample of 2551 horses and tested for the presence of antibodies to the above serovars using the microscopic agglutination test. Samples with a titer $100 were considered positive. Clinical and demographic data were collected on each horse, the farms' management practices and ecology. Logistic regression analysis was used to develop a multivariate indexing system and to identify factors significantly associated with the risk of leptospirosis. Four indices were developed based on the possible sources of exposure: rodent exposure index; wildlife exposure index; soil and water index; and management index. The soil and water index was significantly associated with the risk of exposure to all three serovars. Management was positively associated with L. icterohaemorrhagiae and L. canicola. Density of horses turned out together was positively associated with the risk of exposure to L. grippotyphosa. We concluded that indirect exposure of horses to L. interrogans through contaminated soil and water appears to be significantly associated with the risk of exposure to all three serovars. Management appears to play an important role in the exposure to L. interrogans. Modification of management practices might reduce the horses' risk of exposure and hopefully minimize the human hazards.
Address Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0167-5877 ISBN Medium
Area Expedition Conference
Notes PMID:9762736 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2653
Permanent link to this record
 

 
Author Kida, H.
Title [Ecology of influenza viruses in animals and the mechanism of emergence of new pandemic strains] Type Journal Article
Year 1997 Publication Nippon Rinsho. Japanese Journal of Clinical Medicine Abbreviated Journal Nippon Rinsho
Volume 55 Issue 10 Pages 2521-2526
Keywords Animals; Bird Diseases/transmission; Birds; Horse Diseases/transmission; Horses; Humans; Influenza, Human/transmission/*veterinary; Swine; Swine Diseases/transmission; Zoonoses
Abstract Ecological studies on influenza viruses revealed that the hemagglutinin genes are introduced into new pandemic strains from viruses circulating in migratory ducks through domestic ducks and pigs in southern China. Experimental infection of pigs with 38 avian influenza virus strains with H1-H13 hemagglutinins showed that at least one strain of each HA subtype replicated in the upper respiratory tract of pigs. Co-infection of pigs with a swine virus and with an avian virus generated reassortant viruses. The results indicate that avian viruses of any subtype can contribute genes in the generation of reassortants. Virological surveillance revealed that influenza viruses in waterfowl reservoir are perpetuated year-by-year in the frozen lake water while ducks are absent.
Address Department of Disease Control, Hokkaido University Graduate School of Veterinary Medicine
Corporate Author Thesis
Publisher Place of Publication Editor
Language Japanese Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0047-1852 ISBN Medium
Area Expedition Conference
Notes PMID:9360367 Approved no
Call Number (up) Equine Behaviour @ team @ Serial 2654
Permanent link to this record