|
Records |
Links |
|
Author |
Milinovich, G.J.; Trott, D.J.; Burrell, P.C.; van Eps, A.W.; Thoefner, M.B.; Blackall, L.L.; Al Jassim, R.A.M.; Morton, J.M.; Pollitt, C.C. |
|
|
Title |
Changes in equine hindgut bacterial populations during oligofructose-induced laminitis |
Type |
Journal Article |
|
Year |
2006 |
Publication |
Environmental Microbiology |
Abbreviated Journal |
Environ Microbiol |
|
|
Volume |
8 |
Issue |
5 |
Pages |
885-898 |
|
|
Keywords |
Animal Feed; Animals; Bacteria/classification/*isolation & purification; DNA, Bacterial/analysis; Disease Models, Animal; Feces/microbiology; Foot Diseases/etiology/microbiology/*veterinary; Horse Diseases/*etiology/metabolism/microbiology; Horses; In Situ Hybridization, Fluorescence; Intestines/*microbiology; Oligosaccharides/*administration & dosage/*metabolism; Phylogeny; Polymerase Chain Reaction; RNA, Bacterial/analysis; RNA, Ribosomal, 16S/analysis |
|
|
Abstract |
In the horse, carbohydrate overload is thought to play an integral role in the onset of laminitis by drastically altering the profile of bacterial populations in the hindgut. The objectives of this study were to develop and validate microbial ecology methods to monitor changes in bacterial populations throughout the course of experimentally induced laminitis and to identify the predominant oligofructose-utilizing organisms. Laminitis was induced in five horses by administration of oligofructose. Faecal specimens were collected at 8 h intervals from 72 h before to 72 h after the administration of oligofructose. Hindgut microbiota able to utilize oligofructose were enumerated throughout the course of the experiment using habitat-simulating medium. Isolates were collected and representatives identified by 16S rRNA gene sequencing. The majority of these isolates collected belonged to the genus Streptococcus, 91% of which were identified as being most closely related to Streptococcus infantarius ssp. coli. Furthermore, S. infantarius ssp. coli was the predominant oligofructose-utilizing organism isolated before the onset of lameness. Fluorescence in situ hybridization probes developed to specifically target the isolated Streptococcus spp. demonstrated marked population increases between 8 and 16 h post oligofructose administration. This was followed by a rapid population decline which corresponded with a sharp decline in faecal pH and subsequently lameness at 24-32 h post oligofructose administration. This research suggests that streptococci within the Streptococcus bovis/equinus complex may be involved in the series of events which precede the onset of laminitis in the horse. |
|
|
Address |
Australian Equine Laminitis Research Unit, School of Veterinary Science, The University of Queensland, Queensland, Australia. g.milinovich@uq.edu.au |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
1462-2912 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:16623745 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2625 |
|
Permanent link to this record |
|
|
|
|
Author |
Muscatello, G.; Gilkerson, J.R.; Browning, G.F. |
|
|
Title |
Comparison of two selective media for the recovery, isolation, enumeration and differentiation of Rhodococcus equi |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
|
|
Volume |
119 |
Issue |
2-4 |
Pages |
324-329 |
|
|
Keywords |
|
|
|
Abstract |
The use of selective media to facilitate the isolation of Rhodococcus equi from environmental and clinical samples has aided studies of the ecology of R. equi and the epidemiology of disease caused by R. equi. Here, we compared the efficacy of two selective media (NANAT and modified CAZ-NB) for the recovery of six defined strains of R. equi and for the isolation and enumeration of both avirulent and virulent R. equi from 60 paired soil samples from horse farms using colony blotting and DNA hybridisation. No difference was found between the two media in the recoverability of defined strains of R. equi or the proportion of soil cultures positive for R. equi or virulent R. equi. NANAT medium was significantly less inhibitory of bacterial growth from soil culture compared to mCAZ-NB (P = 0.001), but there was no difference between the media in the number of R. equi colonies recovered. Soil cultured on mCAZ-NB medium yielded a significantly greater number of virulent R. equi colonies than NANAT (P = 0.03). The proportion of R. equi that were virulent in soil cultures on mCAZ-NB (32%) was more than three times that seen in cultures on NANAT (9%). Thus modified CAZ-NB appeared to be a better selective media for studies where the optimal recovery of virulent R. equi is required, such as in studies of the gastrointestinal carriage of virulent R. equi and of subclinically infected foals. |
|
|
Address |
Equine Infectious Disease Laboratory, School of Veterinary Science, The University of Melbourne, Victoria 3010, Australia. mug@unimelb.edu.au |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0378-1135 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17084043 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2621 |
|
Permanent link to this record |
|
|
|
|
Author |
Muscatello, G.; Anderson, G.A.; Gilkerson, J.R.; Browning, G.F. |
|
|
Title |
Associations between the ecology of virulent Rhodococcus equi and the epidemiology of R. equi pneumonia on Australian thoroughbred farms |
Type |
Journal Article |
|
Year |
2006 |
Publication |
Applied and Environmental Microbiology |
Abbreviated Journal |
Appl Environ Microbiol |
|
|
Volume |
72 |
Issue |
9 |
Pages |
6152-6160 |
|
|
Keywords |
Actinomycetales Infections/epidemiology/microbiology/*veterinary; Air Microbiology; Animal Husbandry; Animals; Animals, Newborn; Australia/epidemiology; Colony Count, Microbial; DNA, Bacterial/genetics; Ecosystem; Horse Diseases/epidemiology/*microbiology; Horses; Pneumonia, Bacterial/epidemiology/microbiology/*veterinary; Rhodococcus equi/genetics/isolation & purification/*pathogenicity; Soil Microbiology; Virulence |
|
|
Abstract |
The ecology of virulent strains of Rhodococcus equi on horse farms is likely to influence the prevalence and severity of R. equi pneumonia in foals. This study examined the association between the ecology of virulent R. equi and the epidemiology of R. equi pneumonia by collecting air and soil samples over two breeding seasons (28 farm-year combinations) on Thoroughbred breeding farms with different reported prevalences of R. equi pneumonia. Colony blotting and DNA hybridization were used to detect and measure concentrations of virulent R. equi. The prevalence of R. equi pneumonia was associated with the airborne burden of virulent R. equi (both the concentration and the proportion of R. equi bacteria that were virulent) but was not associated with the burden of virulent R. equi in the soil. Univariable screening and multivariable model building were used to evaluate the effect of environmental and management factors on virulent R. equi burdens. Lower soil moisture concentrations and lower pasture heights were significantly associated with elevated airborne concentrations of virulent R. equi, as were the holding pens and lanes, which typically were sandy, dry, and devoid of pasture cover. Few variables appeared to influence concentrations of virulent R. equi in soil. Acidic soil conditions may have contributed to an elevated proportion of virulent strains within the R. equi population. Environmental management strategies that aim to reduce the level of exposure of susceptible foals to airborne virulent R. equi are most likely to reduce the impact of R. equi pneumonia on endemically affected farms. |
|
|
Address |
School of Veterinary Science, The University of Melbourne, Parkville, Victoria 3010, Australia. mug@unimelb.edu.au |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0099-2240 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:16957241 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2622 |
|
Permanent link to this record |
|
|
|
|
Author |
Kulikova, E.E; Isaeva, A.S.; Rotkina, A.S.; Manykin, A.A.; Letarov, A.V. |
|
|
Title |
Diversity and dynamics of bacteriophages in horse feces |
Type |
Journal Article |
|
Year |
2007 |
Publication |
Microbiology |
Abbreviated Journal |
Microbiology |
|
|
Volume |
76 |
Issue |
2 |
Pages |
271-278 |
|
|
Keywords |
|
|
|
Abstract |
The complex cellulolytic microbial community of the horse intestines is a convenient model for studying the ecology of bacteriophages in natural habitats. Unlike the rumen of the ruminants, this community of the equine large intestine is not subjected to digestion. The inner conditions of the horse gut are much more stable in comparison to other mammals, due to the fact that the horse diet remains almost unchanged and the intervals between food consumption and defecation are much shorter than the whole digestive cycle. The results of preliminary analysis of the structure and dynamics of the viral community of horse feces, which combines direct and culture methods, are presented. In horse fecal samples, we detected more than 60 morphologically distinct phage types, the majority of which were present as a single phage particle. This indicates that the community includes no less than several hundreds of phage types. Some phage types dominated and constituted 5-11% of the total particle count each. The most numerous phage type had an unusual morphology: the tails of its members were extremely long (about 700 nm), flexible, and irretractable, while their heads were 100 nm in diameter. Several other phage types with similar but not identical properties were detected. The total coliphage plaque count of the samples taken from three animals revealed significant fluctuations in the phage titers. During the observation time, the maximum titer ranged within four orders of magnitude (10(3)-10(7) plaque forming units (PFU)/g); the minimum titer ranged within two orders of magnitude. The samples contained two to five morphologically distinct and potentially competitive coliphage types, specific to a single Escherichia coli strain. |
|
|
Address |
|
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
Russian |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0026-3656 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:17583225 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2618 |
|
Permanent link to this record |
|
|
|
|
Author |
Dauphin, G.; Zientara, S.; Zeller, H.; Murgue, B. |
|
|
Title |
West Nile: worldwide current situation in animals and humans |
Type |
Journal Article |
|
Year |
2004 |
Publication |
Comparative Immunology, Microbiology and Infectious Diseases |
Abbreviated Journal |
Comp Immunol Microbiol Infect Dis |
|
|
Volume |
27 |
Issue |
5 |
Pages |
343-355 |
|
|
Keywords |
Americas/epidemiology; Animals; Birds/virology; Culex/*virology; *Disease Outbreaks; Disease Reservoirs; Europe/epidemiology; Horses/virology; Humans; Insect Vectors/*virology; Middle East/epidemiology; West Nile Fever/*epidemiology/*veterinary/virology; West Nile virus/*growth & development |
|
|
Abstract |
West Nile (WN) virus is a mosquito-borne flavivirus that is native to Africa, Europe, and Western Asia. It mainly circulates among birds, but can infect many species of mammals, as well as amphibians and reptiles. Epidemics can occur in rural as well as urban areas. Transmission of WN virus, sometimes involving significant mortality in humans and horses, has been documented at erratic intervals in many countries, but never in the New World until it appeared in New York City in 1999. During the next four summers it spread with incredible speed to large portions of 46 US states, and to Canada, Mexico, Central America and the Caribbean. In many respects, WN virus is an outstanding example of a zoonotic pathogen that has leaped geographical barriers and can cause severe disease in human and equine. In Europe, in the past two decades there have been a number of significant outbreaks in several countries. However, very little is known of the ecology and natural history of WN virus transmission in Europe and most WN outbreaks in humans and animals remain unpredictable and difficult to control. |
|
|
Address |
AFSSA Alfort, UMR1161 (INRA-AFSSA-ENVA), 22 rue Pierre Curie, BP 63, 94703 Maisons-Alfort Cedex, France |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0147-9571 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:15225984 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2635 |
|
Permanent link to this record |
|
|
|
|
Author |
Nelson, D.M.; Gardner, I.A.; Chiles, R.F.; Balasuriya, U.B.; Eldridge, B.F.; Scott, T.W.; Reisen, W.K.; James Maclachlan, N. |
|
|
Title |
Prevalence of antibodies against Saint Louis encephalitis and Jamestown Canyon viruses in California horses |
Type |
Journal Article |
|
Year |
2004 |
Publication |
Comparative Immunology, Microbiology and Infectious Diseases |
Abbreviated Journal |
Comp Immunol Microbiol Infect Dis |
|
|
Volume |
27 |
Issue |
3 |
Pages |
209-215 |
|
|
Keywords |
Animals; Antibodies, Viral/*blood; California/epidemiology; Encephalitis Virus, California/*immunology/isolation & purification; Encephalitis Virus, St. Louis/*immunology/isolation & purification; Encephalitis, St. Louis/epidemiology/immunology/*veterinary/virology; Female; Horse Diseases/epidemiology/immunology/*virology; Horses; Logistic Models; Male; Neutralization Tests/veterinary; Polyomavirus Infections/epidemiology/immunology/*veterinary/virology; Questionnaires; Seroepidemiologic Studies; Tumor Virus Infections/epidemiology/immunology/*veterinary/virology |
|
|
Abstract |
Jamestown Canyon (JC) and Saint Louis encephalitis (SLE) viruses are mosquito-transmitted viruses that have long been present in California. The objective of this study was to determine the seroprevalence of these two viruses in horses prior to the introduction of West Nile (WN) virus. Approximately 15% of serum samples collected in 1998 from 425 horses on 44 equine operations horses throughout California had serum antibodies to JC virus, whereas antibodies were not detected to SLE virus. The results indicate that horses in California were commonly infected prior to 1998 with mosquito-transmitted Bunyaviruses that are identical or closely related to JC virus, but not with SLE virus. The different seroprevalence of SLE and JC viruses in horses likely reflects the unique ecology of each virus, and it is predicted that WN virus will have a wider distribution in California than closely related SLE virus. |
|
|
Address |
Animal and Plant Health Inspection Service, Veterinary Services, U.S. Department of Agriculture, California and Nevada Area Office, 9850 Micron Avenue, Suite E, Sacramento, CA 95827, USA |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0147-9571 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:15001316 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2637 |
|
Permanent link to this record |
|
|
|
|
Author |
Hall, R.A.; Broom, A.K.; Smith, D.W.; Mackenzie, J.S. |
|
|
Title |
The ecology and epidemiology of Kunjin virus |
Type |
Journal Article |
|
Year |
2002 |
Publication |
Current Topics in Microbiology and Immunology |
Abbreviated Journal |
Curr Top Microbiol Immunol |
|
|
Volume |
267 |
Issue |
|
Pages |
253-269 |
|
|
Keywords |
Animals; Culicidae/virology; Ecosystem; Horse Diseases/etiology; Horses; Humans; Insect Vectors; Population Surveillance; West Nile Fever/*epidemiology/*etiology/veterinary; West Nile virus/classification/genetics/immunology/*isolation & purification |
|
|
Abstract |
|
|
|
Address |
Department of Microbiology and Parasitology, School of Molecular and Microbial Sciences, The University of Queensland, St. Lucia, Queensland 4072, Australia |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0070-217X |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:12082993 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2642 |
|
Permanent link to this record |
|
|
|
|
Author |
Endy, T.P.; Nisalak, A. |
|
|
Title |
Japanese encephalitis virus: ecology and epidemiology |
Type |
Journal Article |
|
Year |
2002 |
Publication |
Current Topics in Microbiology and Immunology |
Abbreviated Journal |
Curr Top Microbiol Immunol |
|
|
Volume |
267 |
Issue |
|
Pages |
11-48 |
|
|
Keywords |
Animals; Birds/virology; Climate; Culicidae/virology; Disease Outbreaks/history; Ecosystem; Encephalitis Virus, Japanese/*pathogenicity; Encephalitis, Japanese/*epidemiology/*etiology/history/transmission; History, 20th Century; Horses/virology; Humans; Insect Vectors; Japan/epidemiology; Risk Factors; Swine/virology; Thailand/epidemiology; Viral Vaccines/pharmacology |
|
|
Abstract |
|
|
|
Address |
Department of Virology, United States Army Medical Component, Armed Forces Research Institute of Medical Sciences (USAMC-AFRIMS), 315/6 Rajvithi Road, Bangkok 10400, Thailand |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0070-217X |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:12082986 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2643 |
|
Permanent link to this record |
|
|
|
|
Author |
Holzapfel, W.H.; Botha, S.J. |
|
|
Title |
Physiology of Sporolactobacillus strains isolated from different habitats and the indication of in vitro antagonism against Bacillus species |
Type |
Journal Article |
|
Year |
1988 |
Publication |
International Journal of Food Microbiology |
Abbreviated Journal |
Int J Food Microbiol |
|
|
Volume |
7 |
Issue |
2 |
Pages |
161-168 |
|
|
Keywords |
Animals; Bacillaceae/isolation & purification/*physiology; Bacillus/*physiology; Cattle; *Ecology; Feces/*microbiology; Food Microbiology; Horses; Sewage; Sheep; Water Microbiology |
|
|
Abstract |
In an ecological study only low numbers of Sporolactobacillus were found in habitats such as the faeces of herbivores, the rumen of cattle and the final waste water of an abattoir. Their presence in the final waste water of an abattoir indicates their possible association with food, and, more specifically, with meat. Differences were found in some physiological characteristics. One isolate (L2404) differed from the authentic Sporolactobacillus ATCC 15538 by its inability to ferment inulin, its growth in presence of 6.5% NaCl and in 0.2% tellurite, by the isomer(s) of lactic acid produced and the mol% G + G in the DNA. One Sporolactobacillus isolate (L2407) showed antagonism against Bacillus cereus, Bacillus cereus var, mycoides, Bacillus megaterium and Bacillus subtilis. |
|
|
Address |
Department of Microbiology and Plant Pathology, University of Pretoria, Republic of South Africa |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0168-1605 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:3275317 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2675 |
|
Permanent link to this record |
|
|
|
|
Author |
Hughes, K.L.; Sulaiman, I. |
|
|
Title |
The ecology of Rhodococcus equi and physicochemical influences on growth |
Type |
Journal Article |
|
Year |
1987 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
|
|
Volume |
14 |
Issue |
3 |
Pages |
241-250 |
|
|
Keywords |
Animals; Feces/microbiology; Horses; Hydrogen-Ion Concentration; Rhodococcus/*growth & development; *Soil Microbiology; Temperature |
|
|
Abstract |
Growth of Rhodococcus equi was studied in vitro. Optimal growth occurred under aerobic conditions between pH 7.0 and 8.5, at 30 degrees C. R. equi survived better in a neutral soil (pH 7.3) than it did in two acid soils (pH less than 5.5). It grew substantially better in soils enriched with faeces than in soils alone. Simple organic acids in horse dung, especially acetate and propionate, appear to be important in supporting growth of R. equi in the environment. The ecology of R. equi can be best explained by an environmental cycle allowing its proliferation in dung, influenced by management, grazing behaviour and prevailing climatic conditions. Preventive measures should be aimed at reducing or avoiding focal areas of faecal contamination in the environment. |
|
|
Address |
School of Veterinary Science, University of Melbourne, Parkville, Vic., Australia |
|
|
Corporate Author |
|
Thesis |
|
|
|
Publisher |
|
Place of Publication |
|
Editor |
|
|
|
Language |
English |
Summary Language |
|
Original Title |
|
|
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
|
|
Series Volume |
|
Series Issue |
|
Edition |
|
|
|
ISSN |
0378-1135 |
ISBN |
|
Medium |
|
|
|
Area |
|
Expedition |
|
Conference |
|
|
|
Notes |
PMID:3672866 |
Approved |
no |
|
|
Call Number |
Equine Behaviour @ team @ |
Serial |
2678 |
|
Permanent link to this record |