| Records |
| Author |
Dunn, M.F.; Branlant, G. |
| Title |
Roles of zinc ion and reduced coenzyme in horse liver alcohol dehydrogenase catalysis. The mechanism of aldehyde activation |
Type |
Journal Article |
| Year |
1975 |
Publication |
Biochemistry |
Abbreviated Journal |
Biochemistry |
| Volume |
14 |
Issue |
14 |
Pages |
3176-3182 |
| Keywords |
*Alcohol Oxidoreductases/metabolism; Aldehydes/*pharmacology; Animals; Binding Sites; Enzyme Activation/drug effects; Horses; Hydrogen-Ion Concentration; Kinetics; Liver/enzymology; *NAD/analogs & derivatives/pharmacology; Oxidation-Reduction; Protein Binding; Spectrophotometry; Spectrophotometry, Ultraviolet; Temperature; *Zinc/pharmacology |
| Abstract |
1,4,5,6-Tetrahydronicotinamide adenine dinucleotide (H2NADH) has been investigated as a reduced coenzyme analog in the reaction between trans-4-N,N-dimethylaminocinnamaldehyde (I) (lambdamax 398 nm, epsilonmax 3.15 X 10-4 M-minus 1 cm-minus 1) and the horse liver alcohol dehydrogenase-NADH complex. These equilibrium binding and temperature-jump kinetic studies establish the following. (i) Substitution of H2NADH for NADH limits reaction to the reversible formation of a new chromophoric species, lambdamax 468 nm, epsilonmax 5.8 x 10-4 M-minus 1 cm-minus 1. This chromophore is demonstrated to be structurally analogous to the transient intermediate formed during the reaction of I with the enzyme-NADH complex [Dunn, M. F., and Hutchison, J. S. (1973), Biochemistry 12, 4882]. (ii) The process of intermediate formation with the enzyme-NADH complex is independent of pH over the range 6.13-10.54. Although studies were limited to the pH range 5.98-8.72, a similar pH independence appears to hold for the H2NADH system. (iii) Within the ternary complex, I is bound within van der Waal's contact distance of the coenzyme nicotinamide ring. (iv) Formation of the transient intermediate does not involve covalent modification of coenzyme. Based on these findings, we conclude that zinc ion has a Lewis acid function in facilitating the chemical activation of the aldehyde carbonyl for reduction, and that reduced coenzyme plays a noncovalent effector role in this substrate activating step. |
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English |
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| ISSN |
0006-2960 |
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| Notes |
PMID:238585 |
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no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
3817 |
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| Author |
Saigo, S. |
| Title |
Kinetic and equilibrium studies of alkaline isomerization of vertebrate cytochromes c |
Type |
Journal Article |
| Year |
1981 |
Publication |
Biochimica et Biophysica Acta |
Abbreviated Journal |
Biochim Biophys Acta |
| Volume |
669 |
Issue |
1 |
Pages |
13-20 |
| Keywords |
Amino Acid Sequence; Animals; Cytochrome c Group/*metabolism; Dogs; Hydrogen-Ion Concentration; Isomerism; Kinetics; Vertebrates/metabolism |
| Abstract |
Equilibria and kinetics of alkaline isomerization of seven ferricytochromes c from vertebrates were studied by pH-titration and pH-jump methods in the pH region of 7-12. In the equilibrium behavior, no significant difference was detected among the cytochromes c, whereas marked differences in the kinetic behavior were observed. According to the kinetic behavior of the isomerization, the cytochromes c examined fall into three classes: Group I (horse, sheep, dog and pigeon cytochromes c), Group II (tuna and bonito cytochromes c) and Group III (rhesus monkey cytochrome c). The kinetic results are interpreted in terms of the sequential scheme: Neutral form in equilibrium with fast Transient form in equilibrium with slow Alkaline form where the neutral and alkaline forms are the species stable at neutral and alkaline pH, respectively, and the transient form is a kinetic intermediate. From comparison of the primary sequences of the seven cytochromes c and the classification of these cytochromes c, it is concluded that the amino acid substitution Phe/Tyr at the 46-th position has a major influence on the kinetic behavior. In Group II and III cytochromes c, the ionization of Tyr-46 is suggested to bring about loosening of the heme crevice and thus facilitate the ligand replacement involved in the isomerization. |
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English |
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| ISSN |
0006-3002 |
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| Notes |
PMID:6271238 |
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no |
| Call Number |
refbase @ user @ |
Serial |
3871 |
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| Author |
Wilson, M.T.; Silvestrini, M.C.; Morpurgo, L.; Brunori, M. |
| Title |
Electron transfer kinetics between Rhus vernicifera stellacyanin and cytochrome c (horse heart cytochrome c and Pseudomonas cytochrome c551) |
Type |
Journal Article |
| Year |
1979 |
Publication |
Journal of Inorganic Biochemistry |
Abbreviated Journal |
J Inorg Biochem |
| Volume |
11 |
Issue |
2 |
Pages |
95-100 |
| Keywords |
Animals; Copper; Cytochrome c Group/*metabolism; Electron Transport; Kinetics; Metalloproteins/*metabolism; Plant Proteins/*metabolism; *Plants, Toxic; Pseudomonas aeruginosa/*metabolism; Toxicodendron/*metabolism |
| Abstract |
The electron transfer reactions between Rhus vernicifera stellacyanin and either horse heart cytochrome c or Pseudomonas aeruginosa cytochrome c551 were investigated by rapid reaction techniques. The time course of electron transfer is monophasic under all conditions, and thus consistent with a simple formulation of the reaction. Both stopped-flow and temperature-jump experiments yield equilibrium constants in reasonable agreement with values calculated from the redox potentials. The differences in reaction rate between the two cytochromes and stellacyanin are discussed in terms of the Marcus theory. |
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English |
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Edition |
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| ISSN |
0162-0134 |
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Conference |
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| Notes |
PMID:228006 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
3879 |
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| Author |
Czerlinski, G.H.; Wagner, M.; Erickson, J.O.; Theorell, H. |
| Title |
Chemical relaxation studies on the system liver alcohol dehydrogenase, NADH and imidazole |
Type |
Journal Article |
| Year |
1975 |
Publication |
Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry |
Abbreviated Journal |
Acta Chem Scand B |
| Volume |
29 |
Issue |
8 |
Pages |
797-810 |
| Keywords |
Alcohol Oxidoreductases/*metabolism; Animals; Computers; Hydrogen-Ion Concentration; Imidazoles/*metabolism; Kinetics; Liver/enzymology/*metabolism; Mathematics; Models, Chemical; NAD/*metabolism; Time Factors |
| Abstract |
Several years ago, Theorell and Czerlinski conducted experiments on the system of horse liver alcohol dehydrogenase, reduced nicotinamide adenine dinucleotide and imidazole, using the first version of the temperature jump apparatus with detection of changes in fluorescence. These early experiments were repeated with improved instrumentation and confirmed the early experiments in general terms. However, the improved detection system allowed to measure a slight concentration dependence of the relaxation time of around 3 ms. Furthermore, the chemical relaxation time was smaller than the one determined earlier (by factor 2). The data were evaluated much more rigorously than before, allowing an appropriate interpretation of the results. The observed relaxation time is largely due to rate constants in an interconversion of ternary complexes, which are faster than three (of the four) dissociation rate constants, determined previously by Theorell and McKinley-McKee.1,2 This fact contributed to earlier difficulties of finding any concentration dependence. However, the binding of imidazole to the binary enzyme-coenzyme complex can be made to couple kinetically into the interconversion rate of the two ternary complexes. The observed signal derives largely from the ternary complex(es). A substantial fluorescence signal change is associated with the observed relaxation process, suggesting a relocation of the imidazole in reference to the nicotinamide moiety of the bound coenzyme. Nine models are considered with two types of coupling of pre-equilibria (none-all). Quantitative evaluations favor the model with two ternary complexes connected by an interconversion outside the four-step (bimolecular) cycle. The ternary complex outside the cycle has much higher fluorescence yield than the one inside. The interconversion equilibrium is near unity for imidazole. If it would be shifted very much to the side of the “dead-end” complex (as in isobutyramide?!), stimulating action could not take place. |
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Edition |
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| ISSN |
0302-4369 |
ISBN |
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| Notes |
PMID:882 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
3887 |
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| Author |
Bouchet, A. |
| Title |
[Anatomy lessons on animals] |
Type |
Journal Article |
| Year |
2006 |
Publication |
Histoire des Sciences Medicales |
Abbreviated Journal |
Hist Sci Med |
| Volume |
40 |
Issue |
4 |
Pages |
331-338 |
| Keywords |
Anatomy/education/*history; Animals; Dissection/history; Education, Medical/history; Education, Veterinary/*history; France; History, 16th Century; History, 17th Century; History, 18th Century; History, 19th Century; History, 20th Century; Humans; Schools, Veterinary/history |
| Abstract |
The first anatomical studies were realized on the animal by Galen and Vesalius. Bourgelat created the first veterinarian school in Lyons, then in Paris where the famous dissection of a man on his horse can be seen (Fragonard). The Lafosse dynasty was interested in the study of the horse care and the painter Sollier showed the most beautiful coloured engravings about the horses. A chair of anatomy was created to compare the human and animal anatomy by the school of Jardin des Plantes en 1855. |
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French |
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Original Title |
Les lecons d'anatomie sur les animaux |
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0440-8888 |
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| Notes |
PMID:17526401 |
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no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4000 |
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| Author |
Boissevain, I. |
| Title |
[Animal and human rights in installments] |
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| Year |
2007 |
Publication |
Tijdschrift Voor Diergeneeskunde |
Abbreviated Journal |
Tijdschr Diergeneeskd |
| Volume |
132 |
Issue |
4 |
Pages |
132 |
| Keywords |
Animals; Clinical Competence/*standards; Horse Diseases/*diagnosis; Horses; Humans; Netherlands; Time Factors; Veterinary Medicine/*methods/*standards |
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Dutch |
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Original Title |
Dierenrechten en mensenrechten in termijnen |
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| ISSN |
0040-7453 |
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| Notes |
PMID:17366876 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4018 |
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| Author |
Straub, A. |
| Title |
An intelligent crow beats a lab |
Type |
Journal Article |
| Year |
2007 |
Publication |
Science (New York, N.Y.) |
Abbreviated Journal |
Science |
| Volume |
316 |
Issue |
5825 |
Pages |
688 |
| Keywords |
Animals; *Behavior, Animal; *Cognition; *Crows; Dogs; Intelligence; Memory |
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| ISSN |
1095-9203 |
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| Notes |
PMID:17478698 |
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no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4102 |
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| Author |
Cowley, J.J.; Griesel, R.D. |
| Title |
The effect on growth and behaviour of rehabilitating first and second generation low protein rats |
Type |
Journal Article |
| Year |
1966 |
Publication |
Animal Behaviour. |
Abbreviated Journal |
Anim. Behav. |
| Volume |
14 |
Issue |
4 |
Pages |
506-517 |
| Keywords |
Animals; *Behavior, Animal; Diet Therapy; Dietary Proteins; Female; *Growth; Humans; Intelligence; Learning; Male; Mental Retardation/etiology; Protein Deficiency/*therapy; Rats |
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English |
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| ISSN |
0003-3472 |
ISBN |
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| Notes |
PMID:6008473 |
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no |
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Equine Behaviour @ team @ |
Serial |
4119 |
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| Author |
Levy, J. |
| Title |
The mammalian brain and the adaptive advantage of cerebral asymmetry |
Type |
Journal Article |
| Year |
1977 |
Publication |
Annals of the New York Academy of Sciences |
Abbreviated Journal |
Ann N Y Acad Sci |
| Volume |
299 |
Issue |
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Pages |
264-272 |
| Keywords |
*Adaptation, Physiological; Adaptation, Psychological/physiology; Animals; Behavior, Animal/physiology; Brain/*physiology; Cognition/physiology; Dominance, Cerebral/*physiology; *Evolution; Humans; Intelligence; Perception/physiology |
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English |
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Edition |
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| ISSN |
0077-8923 |
ISBN |
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| Notes |
PMID:280207 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4137 |
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| Author |
Cattell, R.B.; Korth, B. |
| Title |
The isolation of temperament dimensions in dogs |
Type |
Journal Article |
| Year |
1973 |
Publication |
Behavioral Biology |
Abbreviated Journal |
Behav Biol |
| Volume |
9 |
Issue |
1 |
Pages |
15-30 |
| Keywords |
Aggression; Animals; *Behavior, Animal; Biometry; Body Weight; *Dogs; Emotions; Factor Analysis, Statistical; Female; Genetics, Behavioral; Heart Rate; Humans; Intelligence; Male; Models, Psychological; *Personality; Problem Solving; Social Behavior |
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English |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0091-6773 |
ISBN |
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Conference |
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| Notes |
PMID:4738708 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4140 |
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