Records |
Author |
Yokoyama, S.; Radlwimmer, F.B. |
Title |
The molecular genetics of red and green color vision in mammals |
Type |
Journal Article |
Year |
1999 |
Publication |
Genetics |
Abbreviated Journal |
Genetics |
Volume |
153 |
Issue |
2 |
Pages |
919-932 |
Keywords |
Amino Acid Sequence; Animals; Base Sequence; COS Cells; Cats; Color Perception/*genetics; DNA Primers; Deer; Dolphins; *Evolution, Molecular; Goats; Guinea Pigs; Horses; Humans; Mammals/*genetics/physiology; Mice; Molecular Sequence Data; Opsin/biosynthesis/chemistry/*genetics; *Phylogeny; Rabbits; Rats; Recombinant Proteins/biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Sciuridae; Sequence Alignment; Sequence Homology, Amino Acid; Transfection |
Abstract |
To elucidate the molecular mechanisms of red-green color vision in mammals, we have cloned and sequenced the red and green opsin cDNAs of cat (Felis catus), horse (Equus caballus), gray squirrel (Sciurus carolinensis), white-tailed deer (Odocoileus virginianus), and guinea pig (Cavia porcellus). These opsins were expressed in COS1 cells and reconstituted with 11-cis-retinal. The purified visual pigments of the cat, horse, squirrel, deer, and guinea pig have lambdamax values at 553, 545, 532, 531, and 516 nm, respectively, which are precise to within +/-1 nm. We also regenerated the “true” red pigment of goldfish (Carassius auratus), which has a lambdamax value at 559 +/- 4 nm. Multiple linear regression analyses show that S180A, H197Y, Y277F, T285A, and A308S shift the lambdamax values of the red and green pigments in mammals toward blue by 7, 28, 7, 15, and 16 nm, respectively, and the reverse amino acid changes toward red by the same extents. The additive effects of these amino acid changes fully explain the red-green color vision in a wide range of mammalian species, goldfish, American chameleon (Anolis carolinensis), and pigeon (Columba livia). |
Address |
Department of Biology, Syracuse University, Syracuse, New York 13244, USA. syokoyam@mailbox.syr.edu |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0016-6731 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:10511567 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
4063 |
Permanent link to this record |
|
|
|
Author |
Cilnis, M.J.; Kang, W.; Weaver, S.C. |
Title |
Genetic conservation of Highlands J viruses |
Type |
Journal Article |
Year |
1996 |
Publication |
Virology |
Abbreviated Journal |
Virology |
Volume |
218 |
Issue |
2 |
Pages |
343-351 |
Keywords |
Alphavirus/*genetics; Alphavirus Infections/transmission/veterinary/virology; Amino Acid Sequence; Animals; Base Sequence; Conserved Sequence; Disease Outbreaks; Encephalitis, Viral/veterinary/virology; *Evolution, Molecular; Horses; Molecular Sequence Data; Phylogeny; RNA, Viral/genetics; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; Turkeys; Variation (Genetics)/*genetics |
Abstract |
We studied molecular evolution of the mosquito-borne alphavirus Highlands J (HJ) virus by sequencing PCR products generated from 19 strains isolated between 1952 and 1994. Sequences of 1200 nucleotides including portions of the E1 gene and the 3' untranslated region revealed a relatively slow evolutionary rate estimated at 0.9-1.6 x 10(-4) substitutions per nucleotide per year. Phylogenetic trees indicated that all HJ viruses descended from a common ancestor and suggested the presence of one dominant lineage in North America. However, two or more minor lineages probably circulated simultaneously for periods of years to a few decades. Strains isolated from a horse suffering encephalitis, and implicated in a recent turkey outbreak, were not phylogenetically distinct from strains isolated in other locations during the same time periods. Our findings are remarkably similar to those we obtained previously for another North American alphavirus, eastern equine encephalomyelitis virus, with which Highlands J shares primary mosquito and avian hosts, geographical distribution, and ecology. These results support the hypotheses that the duration of the transmission season affects arboviral evolutionary rates and vertebrate host mobility influences genetic diversity. |
Address |
Department of Biology, University of California, San Diego, La Jolla 92093-0116, USA |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0042-6822 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:8610461 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2657 |
Permanent link to this record |
|
|
|
Author |
Chilton, N.B. |
Title |
The use of nuclear ribosomal DNA markers for the identification of bursate nematodes (order Strongylida) and for the diagnosis of infections |
Type |
Journal Article |
Year |
2004 |
Publication |
Animal Health Research Reviews / Conference of Research Workers in Animal Diseases |
Abbreviated Journal |
Anim Health Res Rev |
Volume |
5 |
Issue |
2 |
Pages |
173-187 |
Keywords |
Animals; Birds; Cats; DNA Primers; DNA, Helminth/*analysis; DNA, Ribosomal/*analysis; Dogs; Horses; Molecular Diagnostic Techniques/veterinary; Ruminants; Strongylida/*genetics; Strongylida Infections/diagnosis/*veterinary |
Abstract |
Many bursate nematodes are of major importance to animal health. Animals are often parasitized by multiple species that differ in their prevalence, relative abundance and/or pathogenicity. Implementation of effective management strategies for these parasites requires reliable methods for their detection in hosts, identification to the species level and measurement of intensity of infection. One major problem is the difficulty of accurately identifying and distinguishing many species of bursate nematode because of the remarkable morphological similarity of their eggs and larvae. The inability to identify, with confidence, individual nematodes (irrespective of their life-cycle stage) to the species level by morphological methods has often led to a search for species-specific genetic markers. Studies over the past 15 years have shown that sequences of the internal transcribed spacers of ribosomal DNA provide useful genetic markers, providing the basis for the development of PCR-based diagnostic tools. Such molecular methods represent powerful tools for studying the systematics, epidemiology and ecology of bursate nematodes and, importantly, for the specific diagnosis of infections in animals and humans, thus contributing to improved control and prevention strategies for these parasites. |
Address |
Department of Biology, University of Saskatchewan, 112 Science Place, Saskatoon, Saskatchewan S7N 5E2, Canada. neil.chilton@usask.ca |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
1466-2523 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:15984323 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2628 |
Permanent link to this record |
|
|
|
Author |
Traversa, D.; Giangaspero, A.; Galli, P.; Paoletti, B.; Otranto, D.; Gasser, R.B. |
Title |
Specific identification of Habronema microstoma and Habronema muscae (Spirurida, Habronematidae) by PCR using markers in ribosomal DNA |
Type |
Journal Article |
Year |
2004 |
Publication |
Molecular and Cellular Probes |
Abbreviated Journal |
Mol Cell Probes |
Volume |
18 |
Issue |
4 |
Pages |
215-221 |
Keywords |
Animals; Base Sequence; DNA, Ribosomal/blood/*genetics; Feces/parasitology; Genetic Markers; Horses/*parasitology; Molecular Sequence Data; Muscidae/*genetics; Polymerase Chain Reaction; Spirurida Infections/genetics; Spiruroidea/*genetics; Stomach/*parasitology |
Abstract |
Gastric or cutaneous habronemosis caused by Habronema microstoma Creplin, 1849 and Habronema muscae Carter, 1865 is a parasitic disease of equids transmitted by muscid flies. There is a paucity of information on the epidemiology of this disease, which is mainly due to limitations with diagnosis in the live animal and with the identification of the parasites in the intermediate hosts. To overcome such limitations, a molecular approach, based on the use of genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal DNA, was established for the two species of Habronema. Characterisation of the ITS-2 revealed sequence lengths and G+C contents of 296 bp and 29.5% for H. microstoma, and of 334 bp and 35.9% for H. muscae, respectively. Exploiting the sequence difference (approximately 40%) between the two species of nematode, primers were designed and tested by the polymerase chain reaction (PCR) for their specificity using a panel of control DNA samples from common equid endoparasites, and from host tissues, faeces or muscid flies. Effective amplification from each of the two species of Habronema was achieved from as little as 10 pg of genomic DNA. Hence, this molecular approach allows the specific identification and differentiation of the DNA from H. microstoma and H. muscae, and could thus provide a molecular tool for the specific detection of Habronema DNA (irrespective of developmental stage) from faeces, skin and muscid fly samples. The establishment of this tool has important implications for the specific diagnosis of clinical cases of gastric and cutaneous habronemosis in equids, and for studying the ecology and epidemiology of the two species of Habronema. |
Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0890-8508 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:15271381 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2634 |
Permanent link to this record |
|
|
|
Author |
Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B. |
Title |
Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces |
Type |
Journal Article |
Year |
2004 |
Publication |
Parasitology |
Abbreviated Journal |
Parasitology |
Volume |
129 |
Issue |
Pt 6 |
Pages |
733-739 |
Keywords |
Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics |
Abstract |
Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae. |
Address |
Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0031-1820 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:15648696 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2631 |
Permanent link to this record |
|
|
|
Author |
Burke, D.; Cieplucha, C.; Cass, J.; Russell, F.; Fry, G. |
Title |
Win-shift and win-stay learning in the short-beaked echidna (Tachyglossus aculeatus) |
Type |
Journal Article |
Year |
2002 |
Publication |
Animal Cognition |
Abbreviated Journal |
Anim. Cogn. |
Volume |
5 |
Issue |
2 |
Pages |
79-84 |
Keywords |
Animals; Echidna/*psychology; Ecology; Female; *Learning; *Memory; *Predatory Behavior; Reinforcement (Psychology) |
Abstract |
Numerous previous investigators have explained species differences in spatial memory performance in terms of differences in foraging ecology. In three experiments we attempted to extend these findings by examining the extent to which the spatial memory performance of echidnas (or “spiny anteaters”) can be understood in terms of the spatio-temporal distribution of their prey (ants and termites). This is a species and a foraging situation that have not been examined in this way before. Echidnas were better able to learn to avoid a previously rewarding location (to “win-shift”) than to learn to return to a previously rewarding location (to “win-stay”), at short retention intervals, but were unable to learn either of these strategies at retention intervals of 90 min. The short retention interval results support the ecological hypothesis, but the long retention interval results do not. |
Address |
Department of Psychology, University of Wollongong, Wollongong, NSW 2522, Australia. darren_burke@uow.edu.au |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
1435-9448 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:12150039 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2605 |
Permanent link to this record |
|
|
|
Author |
Gasser, R.B.; Hung, G.-C.; Chilton, N.B.; Beveridge, I. |
Title |
Advances in developing molecular-diagnostic tools for strongyloid nematodes of equids: fundamental and applied implications |
Type |
Journal Article |
Year |
2004 |
Publication |
Molecular and Cellular Probes |
Abbreviated Journal |
Mol Cell Probes |
Volume |
18 |
Issue |
1 |
Pages |
3-16 |
Keywords |
Animals; DNA, Helminth; DNA, Ribosomal/analysis; Equidae/*parasitology; Molecular Diagnostic Techniques/*methods; Parasitic Diseases, Animal/diagnosis; Strongylida/classification/genetics; Strongylida Infections/*diagnosis/epidemiology/etiology/veterinary |
Abstract |
Infections of equids with parasitic nematodes of the order Strongylida (subfamilies Strongylinae and Cyathostominae) are of major veterinary importance. In last decades, the widespread use of drugs against these parasites has led to problems of resistance within the Cyathostominae, and to an increase in their prevalence and intensity of infection. Novel control strategies, based on improved knowledge of parasite biology and epidemiology, have thus become important. However, there are substantial limitations in the understanding of fundamental biological and systematic aspects of these parasites, which have been due largely to limitations in their specific identification and diagnosis using traditional, morphological approaches. Recently, there has been progress in the development of DNA-based approaches for the specific identification of strongyloids of equids for systematic studies and disease diagnosis. The present article briefly reviews information on the classification, biology, pathogenesis, epidemiology of equine strongyloids and the diagnosis of infections, highlights knowledge gaps in these areas, describes recent advances in the use of molecular techniques for the genetic characterisation, specific identification and differentiation of strongyloids of equids as a basis for fundamental investigations of the systematics, population biology and ecology. |
Address |
Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0890-8508 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:15036364 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
2636 |
Permanent link to this record |
|
|
|
Author |
Zhao, C.J.; Qin, Y.H.; Lee, X.H.; Wu, C. |
Title |
Molecular and cytogenetic paternity testing of a male offspring of a hinny |
Type |
Journal Article |
Year |
2006 |
Publication |
Journal of Animal Breeding and Genetics = Zeitschrift fur Tierzuchtung und Zuchtungsbiologie |
Abbreviated Journal |
J Anim Breed Genet |
Volume |
123 |
Issue |
6 |
Pages |
403-405 |
Keywords |
Animals; Cytogenetic Analysis; DNA, Mitochondrial/genetics; Equidae/*genetics; Female; Horses/genetics; Hybridization, Genetic; Male; Microsatellite Repeats; Pedigree; Protamines/genetics; Sexual Behavior, Animal |
Abstract |
An alleged male foal of a female mule, whose sire and grandparents were unknown, was identified for its pedigree. Parentage testing was conducted by comparing polymorphism of 12 microsatellite DNA sites and mitochondrial D-loop sequences of the male foal and the female mule. Both the sequence analysis of species-specific DNA fragments and a cytogenetic analysis were performed to identify the species of the foal and its parents. The results showed that the alleged female mule is actually a hinny, and the male foal, which possesses 62 chromosomes, qualifies as an offspring of the female hinny and a jack donkey. |
Address |
Equine Center, China Agricultural University, Beijing, China |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0931-2668 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:17177697 |
Approved |
no |
Call Number |
|
Serial |
1846 |
Permanent link to this record |
|
|
|
Author |
Macfadden, B.J. |
Title |
Evolution. Fossil horses--evidence for evolution |
Type |
Journal Article |
Year |
2005 |
Publication |
Science (New York, N.Y.) |
Abbreviated Journal |
Science |
Volume |
307 |
Issue |
5716 |
Pages |
1728-1730 |
Keywords |
Animals; Body Size; DNA, Mitochondrial; Diet; *Equidae/anatomy & histology/classification/genetics; *Evolution; Feeding Behavior; *Fossils; *Horses/anatomy & histology/classification/genetics; Paleodontology; Phylogeny; Time; Tooth/anatomy & histology |
Abstract |
|
Address |
Florida Museum of Natural History, University of Florida, Gainesville, FL 32611, USA. bmacfadd@flmnh.ufl.edu |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
1095-9203 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:15774746 |
Approved |
no |
Call Number |
|
Serial |
1892 |
Permanent link to this record |
|
|
|
Author |
Wallner, B.; Brem, G.; Muller, M.; Achmann, R. |
Title |
Fixed nucleotide differences on the Y chromosome indicate clear divergence between Equus przewalskii and Equus caballus |
Type |
Journal Article |
Year |
2003 |
Publication |
Animal Genetics |
Abbreviated Journal |
Anim Genet |
Volume |
34 |
Issue |
6 |
Pages |
453-456 |
Keywords |
Animals; Base Sequence; DNA, Mitochondrial/genetics; Genetic Variation/*genetics; Horses/classification/*genetics; Male; Molecular Sequence Data; Phylogeny; Probability; Species Specificity; Y Chromosome/*genetics |
Abstract |
The phylogenetic relationship between Equus przewalskii and E. caballus is often a matter of debate. Although these taxa have different chromosome numbers, they do not form monophyletic clades in a phylogenetic tree based on mtDNA sequences. Here we report sequence variation from five newly identified Y chromosome regions of the horse. Two fixed nucleotide differences on the Y chromosome clearly display Przewalski's horse and domestic horse as sister taxa. At both positions the Przewalski's horse haplotype shows the ancestral state, in common with the members of the zebra/ass lineage. We discuss the factors that may have led to the differences in mtDNA and Y-chromosomal observations. |
Address |
Institut fur Tierzucht und Genetik, Veterinarmedizinische Universitat Wien, Veterinarplatz, Wien, Austria. wallner@i122server.vu-wien.ac.at |
Corporate Author |
|
Thesis |
|
Publisher |
|
Place of Publication |
|
Editor |
|
Language |
English |
Summary Language |
|
Original Title |
|
Series Editor |
|
Series Title |
|
Abbreviated Series Title |
|
Series Volume |
|
Series Issue |
|
Edition |
|
ISSN |
0268-9146 |
ISBN |
|
Medium |
|
Area |
|
Expedition |
|
Conference |
|
Notes |
PMID:14687077 |
Approved |
no |
Call Number |
Equine Behaviour @ team @ |
Serial |
5038 |
Permanent link to this record |