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Paramastri, Y.; Royo, F.; Eberova, J.; Carlsson, H.-E.; Sajuthi, D.; Fernstrom, A.-L.; Pamungkas, J.; Hau, J. |
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Urinary and fecal immunoglobulin A, cortisol and 11-17 dioxoandrostanes, and serum cortisol in metabolic cage housed female cynomolgus monkeys (Macaca fascicularis) |
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Journal Article |
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2007 |
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Journal of Medical Primatology |
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36 |
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6 |
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355-364 |
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cortisol; cynomolgus monkey; immunoglobulin A; long tailed macaque; Macaca fascicularis; metabolism cage |
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Abstract  |
Background and methods Quantitative enzyme-immunoassays of urinary and fecal immunoglobulin A (IgA), cortisol and 11-17-dioxoandrostanes (11,17-DOA), and serum cortisol in eight metabolic-cage-housed female cynomolgus monkeys were performed. The monkeys were divided into two groups, B and NB. Group B animals were blood sampled every 6 hours, whereas Group NB animals were not handled/blood sampled. Results No differences were recorded between the amounts of feces and urine excreted by the two groups. Group B animals excreted more urinary cortisol than did Group NB animals indicating that restraint-blood sampling resulted in a stress response. Excreted amounts of IgA and 11,17-DOA (urine and feces) did not differ between the groups. Conclusions Urinary cortisol was a reliable marker of the stress associated with repeated blood sampling. Declining amounts of excreted urinary cortisol indicated that cynomolgus monkeys acclimated quickly to repeated blood sampling in metabolism cages. Within and between animal variation in amounts of feces voided demonstrated the importance of expressing fecal markers as ‘amounts excreted per time unit per kg body weight’ rather than just measuring the concentrations in fecal samples. |
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Blackwell Publishing Ltd |
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1600-0684 |
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Equine Behaviour @ team @ |
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5854 |
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Weber-Mzell, D.; Kotanko, P.; Hauer, A.C.; Goriup, U.; Haas, J.; Lanner, N.; Erwa, W.; Ahmaida, I.A.; Haitchi-Petnehazy, S.; Stenzel, M.; Lanzer, G.; Deutsch, J. |
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Title |
Gender, age and seasonal effects on IgA deficiency: a study of 7293 Caucasians |
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Journal Article |
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Year |
2004 |
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European Journal of Clinical Investigation |
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34 |
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3 |
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224-228 |
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Age- and gender-related variability; immunoglobulin A; seasonal variability; serum IgA deficiency |
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Background The frequency of serum IgA deficiency (SIgAD) differs between populations. We examined the prevalence of SIgAD in healthy Caucasians. Materials and methods Serum immunoglobulin A (SIgA) was measured in 7293 volunteers (2264 women, 5029 men) aged 30 ± 14·2 years (mean ± SD; range: 12–66). Serum immunoglobulin A and subnormal SIgA levels were defined by a SIgA level < 0·07 g L-1, and between 0·07 and 0·7 g L-1, respectively. Means were compared by analysis of variance (anova) and analysis of covariance (ancova); frequencies by the χ2 test. Results Fifteen subjects (0·21%; one woman, 14 men) had SIgAD. Subnormal SIgA levels were found in 155 persons (2·13%): 21 females (0·93% of the females) and 134 males (2·66% of the males; difference: 1·74%; 95% CI: 1·12–2·33%; P < 0·001). Males were more likely to have subnormal SIgA levels or SIgAD (odds ratio 3·09, 95% CI: 1·97–4·85). The prevalence of SIgAD and subnormal SIgA was lowest in winter (χ2 = 14·8; P = 0·002; 3 d.f.; and χ2 = 43·2; P < 0·001; 3 d.f., respectively). Serum immunoglobulin A concentrations were significantly higher during winter. Serum immunoglobulin A levels increased with age on average by 0·2 ± 0·06 g L-1 per decade of life (P < 0·001). Taking into account the influence of age, SIgA concentration was lower in females as compared with males. Conclusion The prevalence of SIgAD and subnormal SIgA levels is increased in males. There exists a significant influence of gender, age and seasons on SIgA levels. |
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Blackwell Science Ltd |
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1365-2362 |
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Equine Behaviour @ team @ |
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6127 |
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Nicol, C.J.; Adachi, M.; Akiyama, T.E.; Gonzalez, F.J. |
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Title |
PPARgamma in endothelial cells influences high fat diet-induced hypertension |
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Journal Article |
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Year |
2005 |
Publication |
American journal of hypertension : journal of the American Society of Hypertension |
Abbreviated Journal |
Am J Hypertens |
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18 |
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4 Pt 1 |
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549-556 |
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Administration, Oral; Animals; Antihypertensive Agents/pharmacology; Blood Pressure/drug effects; Diabetes Mellitus, Type 2/physiopathology; Dietary Fats/*administration & dosage/pharmacology; Dose-Response Relationship, Drug; Endothelial Cells/*metabolism; Female; Heart Rate/drug effects; Hypertension/*etiology; Ligands; Male; Mice; Mice, Knockout; PPAR gamma/*metabolism; Sodium Chloride/administration & dosage/pharmacology; Thiazolidinediones/pharmacology |
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BACKGROUND: Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands improve human hypertension. However, the mechanism and site of this effect remains unknown, confounded by PPARgamma expression in many cell types, including endothelial cells (ECs). METHODS: To evaluate the vascular role of PPARgamma we used a conditional null mouse model. Specific disruption of PPARgamma in ECs was created by crossing Tie2-Cre+ transgenic (T2T+) and PPARgamma-floxed (fl/fl) mice to generate PPARgamma (fl/fl)T2T+ (PPARgamma E-null) mice. Conscious 8- to 12-week-old congenic PPARgamma (fl/fl)Cre- (wild type) and PPARgamma E-null mice were examined for changes in systolic blood pressure (BP) and heart rate (HR), untreated, after 2 months of salt-loading (drinking water), and after treatment for 3 months with high fat (HF) diet alone or supplemented during the last 2 weeks with rosiglitazone (3 mg/kg/d). RESULTS: Untreated PPARgamma E-nulls were phenotypically indistinguishable from wild-type littermates. However, compared to similarly treated wild types, HF-treated PPARgamma E-nulls had significantly elevated systolic BP not seen after normal diet or salt-loading. Despite sex-dependent baseline differences, salt-loaded and HF-treated PPARgamma E-nulls of either sex had significantly elevated HR versus wild types. Interestingly, rosiglitazone improved serum insulin levels, but not HF diet-induced hypertension, in PPARgamma E-null mice. CONCLUSIONS: These results suggest that PPARgamma in ECs not only is an important regulator of hypertension and HR under stressed conditions mimicking those arising in type 2 diabetics, but also mediates the antihypertensive effects of rosiglitazone. These data add evidence supporting a beneficial role for PPARgamma-specific ligands in the treatment of hypertension, and suggest therapeutic strategies targeting ECs may prove useful. |
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Laboratory of Metabolism, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892, USA |
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0895-7061 |
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PMID:15831367 |
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refbase @ user @ |
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69 |
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Lansade, L.; Bertrand, M.; Bouissou, M.-F. |
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Title |
Effects of neonatal handling on subsequent manageability, reactivity and learning ability of foals |
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Journal Article |
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Year |
2005 |
Publication |
Applied Animal Behaviour Science |
Abbreviated Journal |
Appl. Anim. Behav. Sci. |
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Volume |
92 |
Issue |
1-2 |
Pages |
143-158 |
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Keywords |
Horse; Neonatal handling; Manageability; Emotional reactivity; Learning-ability; Human-animal relationship |
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Behaviour is an important factor to be taken into account in the various uses of horses. Today horses are mainly used for sport and leisure activities. They should therefore be easy to manage, calm and not fearful. Early handling is known to improve manageability and learning ability and to reduce fearfulness in various species. It has become fashionable in the horse industry to use an early training procedure, referred to as “imprint training”, which is said to produce durable if not permanent effects. However, no studies concerning the long-term effects of such neonatal handling have been carried out in horses. The present study examines the short- and long-term effects of neonatal handling on manageability, general reactivity and learning ability of foals. Twenty-six Welsh foals were studied: 13 were handled daily for 14 days from birth and 13 were non-handled controls. The handling procedure consisted of fitting a halter, gently patting all parts of each foal's body, picking up feet and leading over 40 m. Two days, 3 months, 6 months and 1 year after the end of the handling period, foals underwent behavioural tests to measure their manageability and various aspects of their reactivity. The results showed that neonatal handling has only short-term effects on manageability: 2 days after the handling period, handled animals were significantly easier to handle than controls for the four parameters measured during this test (time to fit a halter, time to pick up feet, walk ratio that is time during which foal walks under constraint/total time measured during leading and number of defensive reactions). Two parameters (time to fit a halter and walk ratio) were still lower in handled foals than in non-handled foals 3 months later and only one 6 months later (walk ratio). One year later there was no difference between groups. In addition, there was no effect of handling on reactivity at any time of testing or in any of the tests (reaction to isolation from conspecifics, presence of a human, presence of a novel object and to a surprise effect). Finally, neonatal handling did not improve the spatial or discriminative learning abilities measured at 14 months of age. To conclude, the effects of neonatal handling are only temporary. |
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refbase @ user @ |
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841 |
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Grubb, T.L.; Foreman, J.H.; Benson, G.J.; Thurmon, J.C.; Tranquilli, W.J.; Constable, P.D.; Olson, W.O.; Davis, L.E. |
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Title |
Hemodynamic effects of calcium gluconate administered to conscious horses |
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Journal Article |
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Year |
1996 |
Publication |
Journal of veterinary internal medicine / American College of Veterinary Internal Medicine |
Abbreviated Journal |
J Vet Intern Med |
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10 |
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6 |
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401-404 |
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Animals; Blood Pressure/drug effects/physiology; Calcium/blood; Calcium Gluconate/administration & dosage/*pharmacology; Cardiac Output/drug effects/physiology; Consciousness/*physiology; Dose-Response Relationship, Drug; Female; Heart Rate/drug effects/physiology; Hemodynamic Processes/*drug effects/physiology; Horses/blood/*physiology; Infusions, Intravenous; Male; Myocardial Contraction/drug effects/physiology; Respiration/drug effects/physiology; Stroke Volume/drug effects/physiology; Time Factors |
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Calcium gluconate was administered to conscious horses at 3 different rates (0.1, 0.2, and 0.4 mg/kg/min for 15 minutes each). Serum calcium concentrations and parameters of cardiovascular function were evaluated. All 3 calcium administration rates caused marked increases in both ionized and total calcium concentrations, cardiac index, stroke index, and cardiac contractility (dP/dtmax). Mean arterial pressure and right atrial pressure were unchanged; heart rate decreased markedly during calcium administration. Ionized calcium concentration remained between 54% and 57% of total calcium concentration throughout the study. We conclude that calcium gluconate can safely be administered to conscious horses at 0.1 to 0.4 mg/kg/min and that administration will result in improved cardiac function. |
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Department of Veterinary Clinical Medicine, University of Illinois at Urbana-Champaign, USA |
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0891-6640 |
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PMID:8947873 |
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refbase @ user @ |
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97 |
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Zentall, T.R.; Galizio, M.; Critchfied, T.S. |
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Categorization, concept learning, and behavior analysis: an introduction |
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Journal Article |
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Year |
2002 |
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Journal of the experimental analysis of behavior |
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J Exp Anal Behav |
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78 |
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3 |
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237-248 |
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Animals; Association Learning; *Concept Formation; *Discrimination Learning; Humans; Language |
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Categorization and concept learning encompass some of the most important aspects of behavior, but historically they have not been central topics in the experimental analysis of behavior. To introduce this special issue of the Journal of the Experimental Analysis of Behavior (JEAB), we define key terms; distinguish between the study of concepts and the study of concept learning; describe three types of concept learning characterized by the stimulus classes they yield; and briefly identify several other themes (e.g., quantitative modeling and ties to language) that appear in the literature. As the special issue demonstrates, a surprising amount and diversity of work is being conducted that either represents a behavior-analytic perspective or can inform or constructively challenge this perspective. |
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Department of Psychology, University of Kentucky, Lexington 40506, USA. zentall@pop.uky.edu |
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0022-5002 |
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PMID:12507002 |
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refbase @ user @ |
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236 |
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Steinhoff-Wagner, J. |
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Title |
Coat Clipping of Horses: A Survey |
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Journal Article |
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2019 |
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Journal of Applied Animal Welfare Science |
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Journal of Applied Animal Welfare Science |
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22 |
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2 |
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171-187 |
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Equine, thermoregulation, shaving, winter pelage removal |
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Coat clipping is a common practice in sport horses; however, timing, purpose, technique, and clips vary widely, as do the management and feeding of a clipped horse. The aim of this study was to collect data regarding common clipping practices. A questionnaire was published online in Germany and contained 32 questions. Four hundred ninety-eight people answered at least one question, and 373 individuals (7% male, 93% female; ages 14–59 years) completed all the questions. Clipped horses were predominantly used as sport horses (68%), and they were either clipped immediately before or during the winter season (88%) or year-round (7%). The clipping date was scheduled according to hair length (52%), sweat amount (47%), and drying time (47%). Participants primarily used two clips: the hunter clip and the blanket clip, both without clipping the head (23% each). The majority of the clipped horses wore a blanket day and night (> 90%). Future studies with observations in the field are needed to support survey data in an effort to develop welfare recommendations for clipping practices utilized with horses. |
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1088-8705 |
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doi: 10.1080/10888705.2018.1454319 |
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Equine Behaviour @ team @ |
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6613 |
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López-Bao, J.V.; Sazatornil, V.; Llaneza, L.; Rodríguez, A. |
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Title |
Indirect Effects on Heathland Conservation and Wolf Persistence of Contradictory Policies that Threaten Traditional Free-Ranging Horse Husbandry |
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2013 |
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Conservation Letters |
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Conservation Letters |
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6 |
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6 |
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448-455 |
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Farmland biodiversity; heathlands; integration of environmental policies; management of livestock carcasses; traditional land uses; wolf conservation |
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Abstract |
Conservation agencies within the European Union promote the restoration of traditional land uses as a cost-effective way to preserve biodiversity outside reserves. Although the European Union pursues the integration of the environment into strategic decision-making, it also dictates sectoral policies that may damage farmland biodiversity. We illustrate this point by outlining the socioeconomic factors that allow the persistence of traditional free-ranging horse husbandry in Galicia, northwestern Spain. Free-ranging Galician mountain ponies provide ecological and socioeconomic services including the prevention of forest fires, the maintenance of heathlands and wolves, and the attenuation of wolf-human conflicts. This traditional livestock system may have persisted because it entails negligible costs for farmers. Wolf predation upon Galician mountain ponies does not threaten farmer's economies and seems to be tolerated better than attacks to more valuable stock. Recently, European Union's regulations on animal welfare, carcass management, or meat production put new economic and administrative burdens on farmers, make free-ranging horse rearing economically unsustainable, and incentivize its abandonment. The aim of the European Union to integrate environmental policies may be successful to preserve farmland biodiversity only through careful anticipation of the side effects of apparently unrelated regulations on the fragile equilibrium that sustain traditional land uses. |
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1755-263x |
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Equine Behaviour @ team @ |
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6211 |
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Gehring, T.M.; VerCauteren, K.C.; Provost, M.L.; Cellar, A.C. |
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Utility of livestock-protection dogs for deterring wildlife from cattle farms |
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Journal Article |
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2010 |
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Wildl. Res. |
Abbreviated Journal |
Wildl. Res. |
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37 |
Issue |
8 |
Pages |
715-721 |
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bovine tuberculosis, coyote, grey wolf, livestock protection dog, mesopredators, white-tailed deer, wildlife damage management. |
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Context. Livestock producers worldwide are negatively affected by livestock losses because of predators and wildlife-transmitted diseases. In the western Great Lakes Region of the United States, this conflict has increased as grey wolf (Canis lupus) populations have recovered and white-tailed deer (Odocoileus virginianus) have served as a wildlife reservoir for bovine tuberculosis (Myobacterium bovis).Aims. We conducted field experiments on cattle farms to evaluate the effectiveness of livestock-protection dogs (LPDs) for excluding wolves, coyotes (C. latrans), white-tailed deer and mesopredators from livestock pastures.Methods. We integrated LPDs on six cattle farms (treatment) and monitored wildlife use with tracking swaths on these farms, concurrent with three control cattle farms during 2005-2008. The amount of time deer spent in livestock pastures was recorded using direct observation.Key results. Livestock pastures protected by LPDs had reduced use by these wildlife compared with control pastures not protected by LPDs. White-tailed deer spent less time in livestock pastures protected by LPDs compared with control pastures not protected by LPDs.Conclusions. Our research supports the theory that LPDs can be an effective management tool for reducing predation and disease transmission. We also demonstrate that LPDs are not limited to being used only with sheep and goats; they can also be used to protect cattle.Implications. On the basis of our findings, we support the use of LPDs as a proactive management tool that producers can implement to minimise the threat of livestock depredations and transmission of disease from wildlife to livestock. LPDs should be investigated further as a more general conservation tool for protecting valuable wildlife, such as ground-nesting birds, that use livestock pastures and are affected by predators that use these pastures. |
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Equine Behaviour @ team @ |
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6575 |
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Author |
Turner, A.; Kirkpatrick, J.F. |
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Effects of immunocontraception on population, longevity and body condition in wild mares (Equus caballus) |
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Journal Article |
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2002 |
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Reproduction (Cambridge, England) Supplement |
Abbreviated Journal |
Reprod Suppl |
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60 |
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187-195 |
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Animals; *Animals, Wild; Antigens/administration & dosage; Body Constitution; Contraception, Immunologic/methods/*veterinary; Egg Proteins/administration & dosage; Female; *Horses; Longevity; Membrane Glycoproteins/administration & dosage; Population Control; Population Dynamics; *Receptors, Cell Surface; Swine; Vaccines, Contraceptive/administration & dosage |
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Abstract |
Contraception is becoming a common approach for the management of captive and wild ungulates yet there are few data for contraceptive effects on entire populations. Management-level treatment of mares with porcine zona pellucida (PZP) vaccine resulted in zero population growth of the Assateague Island wild horse population within 1 year of initiation of treatment. Contraceptive efficacy was 90% for mares treated twice in the first year and annually thereafter. For mares given a single initial inoculation, contraceptive efficacy was 78%. The effort required to achieve zero population growth decreased, as 95, 83 and 84% of all adult mares were treated in each of the first 3 years, compared with 59 and 52% during the last 2 years. Mortality rates for mares and foals after the initiation of management-level treatments decreased below historic and pretreatment mortality rates of approximately 5%. Two new age classes have appeared among treated animals (21-25 years and > 25 years), indicating an increase in longevity among treated animals. Body condition scores for all horses, all adult mares and non-lactating mares increased significantly between summer 1989 and autumn 1999 but did not change significantly in lactating mares. These results provide reliable data for the construction of realistic models for contraceptive management of free-roaming or captive ungulate populations. |
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Assateague Island National Seashore, Berlin, MD 21811, USA |
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English |
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1477-0415 |
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PMID:12220158 |
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refbase @ user @ |
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142 |
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