| Records |
| Author |
Polley, L. |
| Title |
Strongylid parasites of horses: experimental ecology of the free-living stages on the Canadian prairie |
Type |
Journal Article |
| Year |
1986 |
Publication |
American Journal of Veterinary Research |
Abbreviated Journal |
Am J Vet Res |
| Volume |
47 |
Issue |
8 |
Pages |
1686-1693 |
| Keywords |
Animals; Canada; Ecology; Feces; Female; Horse Diseases/*epidemiology/parasitology; Horses; Larva; Ovum/cytology; Seasons; Strongyloides/isolation & purification; Strongyloidiasis/epidemiology/*veterinary |
Abstract  |
Each month for a 1-year period (October through September), equine fecal masses containing eggs of strongylid nematodes were placed outdoors on small grass plots in Saskatchewan, Canada. Thereafter, feces and grass from the plots were sampled after intervals of 1 week or longer, and the strongylid eggs and larvae recovered were counted. These observations were made over a 2-year period. Development of eggs to infective larvae occurred in all experiments, except those established in October, December, and January. Infective larvae from experiments set up in April through September survived that winter. During the summer, there was a gradual build up of infective larvae in the fecal masses, which reached a peak in August and September and then decreased into the winter. These results are discussed in the context of the control of strongylid parasites of horses on the Canadian prairie and in other areas of the world with a similar climate and similar horse management practices. |
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English |
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Edition |
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0002-9645 |
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| Notes |
PMID:3752676 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2682 |
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| Author |
Palme, R. |
| Title |
Measuring fecal steroids: guidelines for practical application |
Type |
Journal Article |
| Year |
2005 |
Publication |
Annals of the New York Academy of Sciences |
Abbreviated Journal |
Ann N Y Acad Sci |
| Volume |
1046 |
Issue |
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Pages |
75-80 |
| Keywords |
Animals; Feces/*chemistry; Immunoassay/methods; Reproducibility of Results; Specimen Handling/methods; Steroids/*analysis |
| Abstract |
During the past 20 years, measuring steroid hormone metabolites in fecal samples has become a widely appreciated technique, because it has proved to be a powerful, noninvasive tool that provides important information about an animal's endocrine status (adrenocortical activity and reproductive status). However, although sampling is relatively easy to perform and free of feedback, a careful consideration of various factors is necessary to achieve proper results that lead to sound conclusions. This article aims to provide guidelines for an adequate application of these techniques. It is meant as a checklist that addresses the main topics of concern, such as sample collection and storage, time delay extraction procedures, assay selection and validation, biological relevance, and some confounding factors. These issues are discussed briefly here and in more detail in other recent articles. |
| Address |
Institute of Biochemistry, Department of Natural Sciences, University of Veterinary Medicine, Veterinaerplatz 1, A-1210 Vienna, Austria. Rupert.Palme@vu-wien.ac.at |
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English |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0077-8923 |
ISBN |
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| Notes |
PMID:16055844 |
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no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4081 |
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| Author |
Keay, J.M.; Singh, J.; Gaunt, M.C.; Kaur, T. |
| Title |
Fecal glucocorticoids and their metabolites as indicators of stress in various mammalian species: a literature review |
Type |
Journal Article |
| Year |
2006 |
Publication |
Journal of zoo and wildlife medicine : official publication of the American Association of Zoo Veterinarians |
Abbreviated Journal |
J Zoo Wildl Med |
| Volume |
37 |
Issue |
3 |
Pages |
234-244 |
| Keywords |
Animals; *Animals, Wild/metabolism; Chromatography, High Pressure Liquid/methods/veterinary; Circadian Rhythm; Conservation of Natural Resources; *Ecosystem; Feces/*chemistry; Glucocorticoids/*analysis/metabolism; Humans; Seasons; Species Specificity; Specimen Handling/methods/veterinary; Stress, Psychological/*metabolism |
| Abstract |
Conservation medicine is a discipline in which researchers and conservationists study and respond to the dynamic interplay between animals, humans, and the environment. From a wildlife perspective, animal species are encountering stressors from numerous sources. With the rapidly increasing human population, a corresponding increased demand for food, fuel, and shelter; habitat destruction; and increased competition for natural resources, the health and well-being of wild animal populations is increasingly at risk of disease and endangerment. Scientific data are needed to measure the impact that human encroachment is having on wildlife. Nonbiased biometric data provide a means to measure the amount of stress being imposed on animals from humans, the environment, and other animals. The stress response in animals functions via glucocorticoid metabolism and is regulated by the hypothalamic-pituitary-adrenal axis. Fecal glucocorticoids, in particular, may be an extremely useful biometric test, since sample collection is noninvasive to subjects and, therefore, does not introduce other variables that may alter assay results. For this reason, many researchers and conservationists have begun to use fecal glucocorticoids as a means to measure stress in various animal species. This review article summarizes the literature on many studies in which fecal glucocorticoids and their metabolites have been used to assess stress levels in various mammalian species. Variations between studies are the main focus of this review. Collection methods, storage conditions, shipping procedures, and laboratory techniques utilized by different researchers are discussed. |
| Address |
Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, 0442 Duck Pond Drive, Blacksburg, Virginia 24061, USA |
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English |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
1042-7260 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:17319120 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
616 |
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| Author |
Carlsson, H.-E.; Lyberg, K.; Royo, F.; Hau, J. |
| Title |
Quantification of stress sensitive markers in single fecal samples do not accurately predict excretion of these in the pig |
Type |
Journal Article |
| Year |
2007 |
Publication |
Research in Veterinary Science |
Abbreviated Journal |
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| Volume |
82 |
Issue |
3 |
Pages |
423-428 |
| Keywords |
Cortisol; Immunoglobulin A; Stress; Pigs; Feces; Animal welfare |
| Abstract |
All feces produced during 24 h were collected from five pigs and cortisol and immunoreactive cortisol metabolites (CICM), and IgA were quantified. Within pigs, the concentrations of CICM and IgA varied extensively between random samples obtained from a single fecal dropping, and deviated in most cases significantly from the true concentration measured in total fecal output (CV 6.7–130%). The CICM and IgA contents varied considerably (CV 8.1–114%) within and between individual fecal droppings from the same pig compared to the total fecal excretion. In conclusion, single random samples could not be used to reliably quantify the total fecal concentration or excretion of CICM or IgA in pigs. Analyses of all feces collected during shorter periods than 24 h did not provide an accurate estimate of the daily excretion of CICM. Thus, the concentration of stress sensitive molecules in random single fecal samples as an indicator of animal welfare should be interpreted with prudence. |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0034-5288 |
ISBN |
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Medium |
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Conference |
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| Notes |
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Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
5853 |
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| Author |
Ganswindt, A.; Palme, R.; Heistermann, M.; Borragan, S.; Hodges, J.K. |
| Title |
Non-invasive assessment of adrenocortical function in the male African elephant (Loxodonta africana) and its relation to musth |
Type |
Journal Article |
| Year |
2003 |
Publication |
General and Comparative Endocrinology |
Abbreviated Journal |
Gen Comp Endocrinol |
| Volume |
134 |
Issue |
2 |
Pages |
156-166 |
| Keywords |
Adrenal Cortex/*metabolism/secretion; Adrenal Cortex Function Tests/methods/*veterinary; Adrenocorticotropic Hormone/physiology; Animals; Carbon Isotopes/diagnostic use; Chromatography, High Pressure Liquid/veterinary; Elephants/*metabolism/urine; Feces/*chemistry; Glucocorticoids/analysis/urine; Hydrocortisone/*analysis/diagnostic use/urine; Immunoenzyme Techniques/methods/veterinary; Male; Reproduction/physiology; Sexual Behavior, Animal/physiology; Stress, Psychological/diagnosis/*physiopathology; Testosterone/*analysis/diagnostic use/urine |
| Abstract |
Adult male elephants periodically show the phenomenon of musth, a condition associated with increased aggressiveness, restlessness, significant weight reduction and markedly elevated androgen levels. It has been suggested that musth-related behaviours are costly and that therefore musth may represent a form of physiological stress. In order to provide data on this largely unanswered question, the first aim of this study was to evaluate different assays for non-invasive assessment of adrenocortical function in the male African elephant by (i) characterizing the metabolism and excretion of [3H]cortisol (3H-C) and [14C]testosterone (14C-T) and (ii) using this information to evaluate the specificity of four antibodies for determination of excreted cortisol metabolites, particularly with respect to possible cross-reactions with androgen metabolites, and to assess their biological validity using an ACTH challenge test. Based on the methodology established, the second objective was to provide data on fecal cortisol metabolite concentrations in bulls during the musth and non-musth condition. 3H-C (1 mCi) and 14C-T (100 microCi) were injected simultaneously into a 16 year old male and all urine and feces collected for 30 and 86 h, respectively. The majority (82%) of cortisol metabolites was excreted into the urine, whereas testosterone metabolites were mainly (57%) excreted into the feces. Almost all radioactive metabolites recovered from urine were conjugated (86% 3H-C and 97% 14C-T). In contrast, 86% and >99% of the 3H-C and 14C-T metabolites recovered from feces consisted of unconjugated forms. HPLC separations indicated the presence of various metabolites of cortisol in both urine and feces, with cortisol being abundant in hydrolysed urine, but virtually absent in feces. Although all antibodies measured substantial amounts of immunoreactivity after HPLC separation of peak radioactive samples and detected an increase in glucocorticoid output following the ACTH challenge, only two (in feces against 3alpha,11-oxo-cortisol metabolites, measured by an 11-oxo-etiocholanolone-EIA and in urine against cortisol, measured by a cortisol-EIA) did not show substantial cross-reactivity with excreted 14C-T metabolites and could provide an acceptable degree of specificity for reliable assessment of glucocorticoid output from urine and feces. Based on these findings, concentrations of immunoreactive 3alpha,11-oxo-cortisol metabolites were determined in weekly fecal samples collected from four adult bulls over periods of 11-20 months to examine whether musth is associated with increased adrenal activity. Results showed that in each male levels of these cortisol metabolites were not elevated during periods of musth, suggesting that in the African elephant musth is generally not associated with marked elevations in glucocorticoid output. Given the complex nature of musth and the variety of factors that are likely to influence its manifestation, it is clear, however, that further studies, particularly on free-ranging animals, are needed before a possible relationship between musth and adrenal function can be resolved. This study also clearly illustrates the potential problems associated with cross-reacting metabolites of gonadal steroids in EIAs measuring glucocorticoid metabolites. This has to be taken into account when selecting assays and interpreting results of glucocorticoid metabolite analysis, not only for studies in the elephant but also in other species. |
| Address |
German Primate Centre, Department of Reproductive Biology, Kellnerweg 4, 37077 Gottingen, Germany. ganswindt@www.dpz.gdwg.de |
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Place of Publication |
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Editor |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0016-6480 |
ISBN |
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Medium |
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Conference |
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| Notes |
PMID:14511986 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4085 |
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| Author |
Barton, M.D.; Hughes, K.L. |
| Title |
Ecology of Rhodococcus equi |
Type |
Journal Article |
| Year |
1984 |
Publication |
Veterinary Microbiology |
Abbreviated Journal |
Vet Microbiol |
| Volume |
9 |
Issue |
1 |
Pages |
65-76 |
| Keywords |
Actinomycetales/growth & development/immunology/*isolation & purification; Animal Husbandry; Animals; Antigens, Bacterial/immunology; Artiodactyla/*microbiology; Australia; Digestive System/microbiology; Ecology; Feces/*microbiology; Horses/*microbiology; Hypersensitivity, Delayed; Rabbits/microbiology; *Soil Microbiology |
| Abstract |
A selective broth enrichment technique was used to study the distribution of Rhodococcus equi in soil and grazing animals. Rhodococcus equi was isolated from 54% of soils examined and from the gut contents, rectal faeces and dung of all grazing herbivorous species examined. Rhodococcus equi was not isolated from the faeces or dung of penned animals which did not have access to grazing. The isolation rate from dung was much higher than from other samples and this was found to be due to the ability of R. equi to multiply more readily in dung. Delayed hypersensitivity tests were carried out on horses, sheep and cattle, but only horses reacted significantly. The physiological characteristics of R. equi and the nature of its distribution in the environment suggested that R. equi is a soil organism. |
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English |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0378-1135 |
ISBN |
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| Notes |
PMID:6719819 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
2688 |
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| Author |
Palme, R.; Rettenbacher, S.; Touma, C.; El-Bahr, S.M.; Mostl, E. |
| Title |
Stress hormones in mammals and birds: comparative aspects regarding metabolism, excretion, and noninvasive measurement in fecal samples |
Type |
Journal Article |
| Year |
2005 |
Publication |
Annals of the New York Academy of Sciences |
Abbreviated Journal |
Ann N Y Acad Sci |
| Volume |
1040 |
Issue |
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Pages |
162-171 |
| Keywords |
Adrenal Glands/chemistry/metabolism; Animals; Birds; Catecholamines/analysis/chemistry/*metabolism; Feces/*chemistry; Glucocorticoids/analysis/chemistry/*metabolism; Hormones/analysis/metabolism; Mammals; Species Specificity; Stress/*metabolism |
| Abstract |
A multitude of endocrine mechanisms are involved in coping with challenges. Front-line hormones to overcome stressful situations are glucocorticoids (GCs) and catecholamines (CAs). These hormones are usually determined in plasma samples as parameters of adrenal activity and thus of disturbance. GCs (and CAs) are extensively metabolized and excreted afterwards. Therefore, the concentration of GCs (or their metabolites) can be measured in various body fluids or excreta. Above all, fecal samples offer the advantages of easy collection and a feedback-free sampling procedure. However, large differences exist among species regarding the route and time course of excretion, as well as the types of metabolites formed. Based on information gained from radiometabolism studies (reviewed in this paper), we recently developed and successfully validated different enzyme immunoassays that enable the noninvasive measurement of groups of cortisol or corticosterone metabolites in animal feces. The determination of these metabolites in fecal samples can be used as a powerful tool to monitor GC production in various species of domestic, wildlife, and laboratory animals. |
| Address |
Institute of Biochemistry, Department of Natural Sciences, University of Veterinary Medicine, Vienna, Austria. rupert.palme@vu-wien.ac.at |
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English |
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Series Issue |
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Edition |
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0077-8923 |
ISBN |
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| Notes |
PMID:15891021 |
Approved |
no |
| Call Number |
Equine Behaviour @ team @ |
Serial |
4083 |
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| Author |
Alexander, F. |
| Title |
The effect of diuretics on the faecal excretion of water and electrolytes in horses |
Type |
Journal Article |
| Year |
1977 |
Publication |
British journal of pharmacology |
Abbreviated Journal |
Br J Pharmacol |
| Volume |
60 |
Issue |
4 |
Pages |
589-593 |
| Keywords |
Animals; Body Water/*metabolism; Chlorides/metabolism; Electrolytes/*metabolism; Feces/*analysis; Furosemide/*pharmacology; Horses; Hydrochlorothiazide/*pharmacology; Male; Potassium/metabolism; Sodium/metabolism; Time Factors |
| Abstract |
1. The effect on plasma, urinary and faecal electrolytes of frusemide and hydrochlorthiazide was measured in ponies, mean weight 180 kg. 2. The rapid loss in urine of large quantities of sodium had only a small effect on plasma sodium concentration. 3. Faecal sodium excretion was increased substantially after the administration of frusemide. 4. Frusemide increased faecal potassium during the 48 h following administration and faecal water in the 24/48 h period. It also produced a hypopotassaemia. 5. Hydrochlorthiazide increased faecal chloride during the 24 h after administration. 6. Frusemide increased the intestinal transit time of both liquid (polyethylene glycol) and particulate (Cr2O3) markers. |
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English |
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Edition |
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0007-1188 |
ISBN |
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Conference |
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| Notes |
PMID:907872 |
Approved |
no |
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refbase @ user @ |
Serial |
111 |
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| Author |
Alexander, F.; Davies, M.E. |
| Title |
Studies on vitamin B12 in the horse |
Type |
Journal Article |
| Year |
1969 |
Publication |
The British veterinary journal |
Abbreviated Journal |
Br. Vet. J. |
| Volume |
125 |
Issue |
4 |
Pages |
169-176 |
| Keywords |
Animals; Biological Assay; Cattle; Chickens; Feces/analysis; Horses/*metabolism; Humans; Lactobacillus/metabolism; Rabbits; Rats; Sheep; Urine/analysis; Vitamin B 12/*analysis |
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English |
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Edition |
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0007-1935 |
ISBN |
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| Notes |
PMID:5814055 |
Approved |
no |
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refbase @ user @ |
Serial |
116 |
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| Author |
Pitchford, R.J.; Visser, P.S.; du Toit, J.F.; de Pienaar, U.V.; Young, E. |
| Title |
Observations on the ecology of Schistosoma mattheei Veglia & Le Roux, 1929, in portion of the Kruger National Park and surrounding area using a new quantitative technique for egg output |
Type |
Journal Article |
| Year |
1973 |
Publication |
Journal of the South African Veterinary Association |
Abbreviated Journal |
J S Afr Vet Assoc |
| Volume |
44 |
Issue |
4 |
Pages |
405-420 |
| Keywords |
Animals; Artiodactyla; Buffaloes; Cattle; Cattle Diseases/epidemiology; Dog Diseases/epidemiology; Dogs; Feces; Goats; Haplorhini; Horse Diseases/epidemiology; Horses; Humans; Methods; Monkey Diseases/epidemiology; Papio; Parasite Egg Count; Schistosomiasis/epidemiology/*veterinary; Sheep; Sheep Diseases/epidemiology; South Africa; Swine; Swine Diseases/epidemiology |
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English |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
1019-9128 |
ISBN |
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| Notes |
PMID:4212207 |
Approved |
no |
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Equine Behaviour @ team @ |
Serial |
2711 |
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