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Author | Miksovska, J.; Larsen, R.W. | ||||
Title | Photothermal studies of pH induced unfolding of apomyoglobin | Type | Journal Article | ||
Year | 2003 | Publication | Journal of Protein Chemistry | Abbreviated Journal | J Protein Chem |
Volume | 22 | Issue | 4 | Pages | 387-394 |
Keywords | Acoustics; Animals; Apoproteins/*chemistry/metabolism; Circular Dichroism; Horses; Myocardium/chemistry; Myoglobin/*chemistry/metabolism; Photolysis; Protein Conformation/radiation effects; Protein Denaturation/radiation effects; *Protein Folding; Temperature; Thermodynamics | ||||
Abstract | Conformational dynamic and enthalpy changes associated with pH induced unfolding of apomyoglobin were studied using photoacoustic calorimetry and photothermal beam deflection methods. The transition between the native state and the I intermediate was induced by a nanosecond pH jump from o-nitrobenzaldehyde photolysis. Deconvolution of photoacoustic waves indicates two kinetic processes. The fast phase (T < 50 ns) is characterized by a volume expansion of 8.8 ml mol(-1). This process is followed by a volume contraction of about -22 ml mol(-1) (tau approximately 500 ns). Photothermal beam deflection measurements do not reveal any volume changes on the time scale between approximately 100 micros and 5 ms. We associate the volume contraction with structural changes occurring during the transition between the native state and the I intermediate. The lack of any processes on the ms time scale may indicate the absence of structural events involving larger conformational changes of apomyoglobin after the pH jump. | ||||
Address | Department of Chemistry, University of South Florida, Tampa, Florida 33620, USA | ||||
Corporate Author | Thesis | ||||
Publisher | Place of Publication | Editor | |||
Language | English | Summary Language | Original Title | ||
Series Editor | Series Title | Abbreviated Series Title | |||
Series Volume | Series Issue | Edition | |||
ISSN | 0277-8033 | ISBN | Medium | ||
Area | Expedition | Conference | |||
Notes | PMID:13678303 | Approved | no | ||
Call Number | Equine Behaviour @ team @ | Serial | 3780 | ||
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