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Author Barros, A.T.
Title Seasonality and relative abundance of Tabanidae (Diptera) captured on horses in the Pantanal, Brazil Type Journal Article
Year 2001 Publication Memorias do Instituto Oswaldo Cruz Abbreviated Journal (up) Mem Inst Oswaldo Cruz
Volume 96 Issue 7 Pages 917-923
Keywords Animals; Brazil; Climate; Diptera/classification/*physiology; Ecology; Horses/*parasitology; Population Dynamics; Seasons; Species Specificity
Abstract Once a month, from June 1992 to May 1993, collections of tabanids on horse were conducted in the Nhecolandia, Pantanal State of Mato Grosso do Sul, Brazil. Tabanid catches using hand nets were conducted from sunrise to sunset at grassland and cerradao (dense savanna) habitats. A total of 3,442 tabanids from 21 species,12 genera, and 3 subfamilies were collected. Although species abundance varied seasonally depending on habitat, no habitat specificity was observed for the most abundant species. In the grassland, 1,625 (47.2%) tabanids belonging to 19 species were collected, while 1,817 (52.8%) tabanids from 17 species were caught in the cerradao. The number of tabanid species varied from 7 during winter (July/August) to 15 in the spring (October). Tabanus importunus (56%) was the most abundant species, followed by T. occidentalis (8.2%), and T. claripennis (8.1%). The tabanid peak, in October, coincided with the beginning of the rainy season. The population peak of most species, including those with higher vector potential, suggests that the rainy season can be considered as the period of potentially higher risk of mechanical transmission of pathogens by tabanids to horses in the region.
Address Embrapa Pantanal, 79320-900 Corumba, MS, Brasil. thadeu@cpap.embrapa.br
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0074-0276 ISBN Medium
Area Expedition Conference
Notes PMID:11685255 Approved no
Call Number Equine Behaviour @ team @ Serial 2644
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Author Traversa, D.; Otranto, D.; Iorio, R.; Giangaspero, A.
Title Molecular characterization of Thelazia lacrymalis (Nematoda, Spirurida) affecting equids: a tool for vector identification Type Journal Article
Year 2005 Publication Molecular and Cellular Probes Abbreviated Journal (up) Mol Cell Probes
Volume 19 Issue 4 Pages 245-249
Keywords Animals; Horse Diseases/parasitology; Horses/*parasitology; Insect Vectors/*parasitology; Muscidae/*parasitology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Spirurida Infections/parasitology/veterinary; Thelazioidea/chemistry/*genetics
Abstract Equine thelaziosis caused by the eyeworm Thelazia lacrymalis is a parasitic disease transmitted by muscid flies. Although equine thelaziosis is known to have worldwide distribution, information on the epidemiology and presence of the intermediate hosts of T. lacrymalis is lacking. In the present work, a PCR-RFLP based assay on the first and/or second internal transcribed spacer (ITS1 and ITS2) of ribosomal DNA was developed for the detection of T. lacrymalis DNA in its putative vector(s). The sensitivity of the technique was also assessed. The restriction patterns obtained readily differentiated T. lacrymalis from four species of Musca (Diptera, Muscidae) (i.e. Musca autumnalis, Musca domestica, Musca larvipara and Musca osiris), which are potential vectors of equine eyeworms. The molecular assay presented herein is a useful tool to identify the intermediate host(s) of T. lacrymalis in natural conditions and to study its/their ecology and epidemiology.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Piazza Aldo Moro 45, 64100 Teramo, Italy. dtraversa@unite.it
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0890-8508 ISBN Medium
Area Expedition Conference
Notes PMID:16038792 Approved no
Call Number Equine Behaviour @ team @ Serial 2626
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Author Traversa, D.; Giangaspero, A.; Galli, P.; Paoletti, B.; Otranto, D.; Gasser, R.B.
Title Specific identification of Habronema microstoma and Habronema muscae (Spirurida, Habronematidae) by PCR using markers in ribosomal DNA Type Journal Article
Year 2004 Publication Molecular and Cellular Probes Abbreviated Journal (up) Mol Cell Probes
Volume 18 Issue 4 Pages 215-221
Keywords Animals; Base Sequence; DNA, Ribosomal/blood/*genetics; Feces/parasitology; Genetic Markers; Horses/*parasitology; Molecular Sequence Data; Muscidae/*genetics; Polymerase Chain Reaction; Spirurida Infections/genetics; Spiruroidea/*genetics; Stomach/*parasitology
Abstract Gastric or cutaneous habronemosis caused by Habronema microstoma Creplin, 1849 and Habronema muscae Carter, 1865 is a parasitic disease of equids transmitted by muscid flies. There is a paucity of information on the epidemiology of this disease, which is mainly due to limitations with diagnosis in the live animal and with the identification of the parasites in the intermediate hosts. To overcome such limitations, a molecular approach, based on the use of genetic markers in the second internal transcribed spacer (ITS-2) of ribosomal DNA, was established for the two species of Habronema. Characterisation of the ITS-2 revealed sequence lengths and G+C contents of 296 bp and 29.5% for H. microstoma, and of 334 bp and 35.9% for H. muscae, respectively. Exploiting the sequence difference (approximately 40%) between the two species of nematode, primers were designed and tested by the polymerase chain reaction (PCR) for their specificity using a panel of control DNA samples from common equid endoparasites, and from host tissues, faeces or muscid flies. Effective amplification from each of the two species of Habronema was achieved from as little as 10 pg of genomic DNA. Hence, this molecular approach allows the specific identification and differentiation of the DNA from H. microstoma and H. muscae, and could thus provide a molecular tool for the specific detection of Habronema DNA (irrespective of developmental stage) from faeces, skin and muscid fly samples. The establishment of this tool has important implications for the specific diagnosis of clinical cases of gastric and cutaneous habronemosis in equids, and for studying the ecology and epidemiology of the two species of Habronema.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0890-8508 ISBN Medium
Area Expedition Conference
Notes PMID:15271381 Approved no
Call Number Equine Behaviour @ team @ Serial 2634
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Author Gasser, R.B.; Hung, G.-C.; Chilton, N.B.; Beveridge, I.
Title Advances in developing molecular-diagnostic tools for strongyloid nematodes of equids: fundamental and applied implications Type Journal Article
Year 2004 Publication Molecular and Cellular Probes Abbreviated Journal (up) Mol Cell Probes
Volume 18 Issue 1 Pages 3-16
Keywords Animals; DNA, Helminth; DNA, Ribosomal/analysis; Equidae/*parasitology; Molecular Diagnostic Techniques/*methods; Parasitic Diseases, Animal/diagnosis; Strongylida/classification/genetics; Strongylida Infections/*diagnosis/epidemiology/etiology/veterinary
Abstract Infections of equids with parasitic nematodes of the order Strongylida (subfamilies Strongylinae and Cyathostominae) are of major veterinary importance. In last decades, the widespread use of drugs against these parasites has led to problems of resistance within the Cyathostominae, and to an increase in their prevalence and intensity of infection. Novel control strategies, based on improved knowledge of parasite biology and epidemiology, have thus become important. However, there are substantial limitations in the understanding of fundamental biological and systematic aspects of these parasites, which have been due largely to limitations in their specific identification and diagnosis using traditional, morphological approaches. Recently, there has been progress in the development of DNA-based approaches for the specific identification of strongyloids of equids for systematic studies and disease diagnosis. The present article briefly reviews information on the classification, biology, pathogenesis, epidemiology of equine strongyloids and the diagnosis of infections, highlights knowledge gaps in these areas, describes recent advances in the use of molecular techniques for the genetic characterisation, specific identification and differentiation of strongyloids of equids as a basis for fundamental investigations of the systematics, population biology and ecology.
Address Department of Veterinary Science, The University of Melbourne, 250 Princes Highway, Werribee, Victoria 3030, Australia. robinbg@unimelb.edu.au
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0890-8508 ISBN Medium
Area Expedition Conference
Notes PMID:15036364 Approved no
Call Number Equine Behaviour @ team @ Serial 2636
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Author Traversa, D.; Giangaspero, A.; Iorio, R.; Otranto, D.; Paoletti, B.; Gasser, R.B.
Title Semi-nested PCR for the specific detection of Habronema microstoma or Habronema muscae DNA in horse faeces Type Journal Article
Year 2004 Publication Parasitology Abbreviated Journal (up) Parasitology
Volume 129 Issue Pt 6 Pages 733-739
Keywords Animals; DNA, Helminth/*analysis; DNA, Ribosomal Spacer/*chemistry; Feces/*chemistry; Female; Horse Diseases/*diagnosis/parasitology; Horses; Male; Polymerase Chain Reaction/*methods; Species Specificity; Spirurida Infections/diagnosis/*veterinary; Spiruroidea/*genetics
Abstract Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) are parasitic nematodes which infect the stomach and/or skin of equids. The accurate diagnosis of gastric habronemosis is central to studying its epidemiology, but data on its distribution and prevalence are lacking, mainly due to the limitations of clinical and coprological diagnosis in live horses. To overcome this constraint, a two-step, semi-nested PCR-based assay was validated (utilizing genetic markers in the nuclear ribosomal DNA) for the specific amplification of H. microstoma or H. muscae DNA from the faeces from horses (n = 46) whose gastrointestinal parasite status had been determined at autopsy and whose faeces were examined previously using a conventional parasitological approach. Of these horses examined at autopsy, some harboured adults of either H. microstoma (n= 19) or H. muscae (n =4), and others (n = 7) harboured both species. Most of them were also infected with other parasites, including strongylid nematodes (subfamilies Cyathostominae and Strongylinae), bots and/or cestodes; there was no evidence of metazoan parasites in 2 horses. Larvated spirurid eggs were detected in the faeces of 1 of the 30 horses (3.3 %) shown to be infected with Habronema at autopsy. For this set of 46 samples, the PCR assay achieved a diagnostic specificity of 100 % and a sensitivity of approximately 97 % (being able to specifically detect as little as approximately 0.02 fg of Habronema DNA). The specificity of the assay was also tested using a panel of control DNA samples representing horse, the gastric spirurid Draschia megastoma and 26 other species of parasites from the alimentary tract of the horse. H. microstoma, H. muscae and D. megastoma could be readily differentiated from one another based on the sizes of their specific amplicons in the PCR. The results of this study showed that the performance of the PCR for the diagnosis of gastric habronemosis was similar to that of autopsy but substantially better than the traditional coprological examination procedure used. The ability to specifically diagnose gastric habronemosis in equids should have important implications for investigating the epidemiology and ecology of H. microstoma and H. muscae.
Address Department of Biomedical Comparative Sciences, Faculty of Veterinary Medicine, University of Teramo, Teramo, Italy. traversa@unite.it
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1820 ISBN Medium
Area Expedition Conference
Notes PMID:15648696 Approved no
Call Number Equine Behaviour @ team @ Serial 2631
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Author Giangaspero, A.; Traversa, D.; Otranto, D.
Title [Ecology of Thelazia spp. in cattle and their vectors in Italy] Type Journal Article
Year 2004 Publication Parassitologia Abbreviated Journal (up) Parassitologia
Volume 46 Issue 1-2 Pages 257-259
Keywords Animals; Cattle/parasitology; Cattle Diseases/epidemiology/*parasitology/transmission; Disease Transmission, Horizontal; Dog Diseases/epidemiology/parasitology/transmission; Dogs/parasitology; Ecosystem; Eye Infections, Parasitic/epidemiology/transmission/*veterinary; Horse Diseases/epidemiology/parasitology/transmission; Horses/parasitology; Humans; Insect Vectors/*parasitology; Italy/epidemiology; Muscidae/*parasitology; Species Specificity; Spirurida Infections/epidemiology/transmission/*veterinary; Thelazioidea/classification/*isolation & purification
Abstract The genus Thelazia (Spirurida, Thelaziidae) includes a cosmopolitan group of eyeworm spirurids responsible for ocular infections in domestic and wild animals and transmitted by different species of muscids. Bovine thelaziosis is caused by Thelazia rhodesi Desmarest 1828, Thelazia gulosa Railliet & Henry 1910, and Thelazia skrjabini Erschow 1928, which occur in many countries; T. gulosa and T. skrjabini have been reported mainly in the New World, while T. rhodesi is particularly common in the Old World. In Italy, T. rhodesi was reported in southern regions a long time ago and, recently, T. gulosa and T. skrjabini have been identified in autochthonous cattle first in Apulia and then in Sardinia. Thirteen species of Musca are listed as intermediate hosts of eyeworms, but only Musca autumnalis and Musca larvipara have been demonstrated to act as vectors of Thelazia in the ex-URSS, North America, ex-Czechoslovakia and more recently in Sweden. In Italy, after the reports of T. gulosa and T. skrjabini in southern regions, the intermediate hosts of bovine eyeworms were initially only suspected as the predominant secretophagous Muscidae collected from the periocular region of cattle with thelaziosis were the face flies, M. autumnalis and M. larvipara, followed by Musca osiris, Musca tempestiva and Musca domestica. The well-known constraints in the identification of immature eyeworms to species by fly dissection and also the time-consuming techniques used constitute important obstacles to epidemiological field studies (i.e. vector identification and/or role, prevalence and pattern of infection in flies, etc.). Molecular studies have recently permitted to further investigations into this area. A PCR-RFLP analysis of the ribosomal ITS-1 sequence was developed to differentiate the 3 species of Thelazia (i.e. T. gulosa, T. rhodesi and T. skrjabini) found in Italy, then a molecular epidemiological survey has recently been carried out in field conditions throughout five seasons of fly activity and has identified the role of M. autumnalis, M. larvipara, M. osiris and M. domestica as vectors of T. gulosa and of M. autumnalis and M. larvipara of T. rhodesi. Moreover, M. osiris was described, for the first time, to act as a vector of T. gulosa and M. larvipara of T. gulosa and T. rhodesi. The mean prevalence in the fly population examined was found to be 2.86%. The molecular techniques have opened new perspectives for further research on the ecology and epidemiology not only of Thelazia in cattle but also of other autochthonous species of Thelazia which have been also recorded in Italy, such as Thelazia callipaeda, which is responsible for human and canid ocular infection and Thelazia lacrymalis, the horse eyeworm whose epidemiological molecular studies are in progress.
Address Dipartimento PR.I.M.E., Universita degli Studi di Foggia
Corporate Author Thesis
Publisher Place of Publication Editor
Language Italian Summary Language Original Title Ecologia di Thelazia spp. e dei vettori in Italia
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0048-2951 ISBN Medium
Area Expedition Conference
Notes PMID:15305729 Approved no
Call Number Equine Behaviour @ team @ Serial 2633
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Author No authors listed
Title Workshop on the geographic spread of Aedes albopictus in Europe and the concern among public health authorities. Proceedings of a workshop held at the Istituto Superiore di Sanita, Rome, Italy, 19-20 December 1994 Type Conference Article
Year 1995 Publication Parassitologia Abbreviated Journal (up) Parassitologia
Volume 37 Issue 2-3 Pages 87-90
Keywords *Aedes/growth & development/parasitology/virology; African horse sickness virus; Animals; Commerce; Dengue Virus; Dirofilaria; Disease Reservoirs; Ecology; Europe; Humans; *Insect Vectors/growth & development/parasitology/virology; Italy; *Mosquito Control/methods/organization & administration; Public Health; Rift Valley fever virus
Abstract
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0048-2951 ISBN Medium
Area Expedition Conference
Notes PMID:8778669 Approved no
Call Number Equine Behaviour @ team @ Serial 2659
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Author Dvoinos, G.M.; Kharchenko, V.A.; Zviagnitsova, N.S.
Title The characteristics of the helminth community in the Turkmen kulan (Equus hemionus) Type Journal Article
Year 1992 Publication Parazitologiia Abbreviated Journal (up) Parazitologiia
Volume 26 Issue 3 Pages 246-251
Keywords Animals; Female; Helminthiasis/parasitology; Helminthiasis, Animal; Helminths/classification/*isolation & purification; Male; Perissodactyla/*parasitology; Turkmenistan; Ukraine
Abstract The helminth fauna of 24 kulans from Askaniya-Nova and Badkhyz was studied. 42 species of helminths were found, 34 of which belong to strongylids. The helminth species composition of kulan is similar to that of other species of horses. This is a result of an intensive parasite exchange in the historical past when numerous populations of different Equidae species made long seasonal migrations over steppe inter-river lands of Asia and grazed for some time on common pastures.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Russian Summary Language Original Title K kharakteristike soobshchestva gel'mintov turkmenskogo kulana (Equus hemionus)
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1847 ISBN Medium
Area Expedition Conference
Notes PMID:1408368 Approved no
Call Number Equine Behaviour @ team @ Serial 2285
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Author Konstantinov, S.A.; Veselkin, A.G.
Title [The intensity and efficiency of a gadfly attack on cattle depending on the number and location of the animals in the herd] Type Journal Article
Year 1989 Publication Parazitologiia Abbreviated Journal (up) Parazitologiia
Volume 23 Issue 1 Pages 3-10
Keywords Animals; Cattle/*parasitology; Diptera/*physiology; Ecology; Insect Bites and Stings/parasitology/*veterinary; Russia
Abstract The effect of group was studied on cattle being attacked by horse flies of three genera. The method of simultaneous registrations of attacking horse flies in herds of 8 to 100 animals and on single cows was used. It has been shown that the effect of group reveals itself only when animals in the herd reach a certain minimum number, the effect rate depending on peculiarities of attacking of a given species of bloodsuckers, such as a part of responding individuals, distance of an attack, duration of contact with an object. These parameters tend to change with increasing number of animals in the herd. Therefore differences in the intensity of attacks on herds with different cattle stock cannot be explained proceeding only from differences in the occupied areas. The number of attacking horse flies decreases from the periphery of the herd to its centre and is not the same in different parts of the periphery. The effectiveness of attacking, ie the part of sucking individuals of a given species (genus) from the number of horse flies attacking for a definite period of time, is the highest in a large herd and increases in its ranges from the periphery to the centre. This dependence leads to a more even distribution of sucking individuals as compared to attacking ones.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Russian Summary Language Original Title Intensivnost' i effektivnost' napadeniia slepnei na krupnyi rogatyi skot v zavisimosti ot chisla i mestopolozheniia zhivotnykh v stade
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1847 ISBN Medium
Area Expedition Conference
Notes PMID:2524028 Approved no
Call Number Equine Behaviour @ team @ Serial 2674
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Author Enileeva, N.K.
Title [Ecological characteristics of horse stomach botflies in Uzbekistan] Type Journal Article
Year 1987 Publication Parazitologiia Abbreviated Journal (up) Parazitologiia
Volume 21 Issue 4 Pages 577-579
Keywords Animals; Diptera/*physiology; Ecology; Female; Flight, Animal; Horses/*parasitology; Larva/physiology; Male; Population Dynamics; Uzbekistan
Abstract The paper describes the flight periods and dynamics of abundance of horse botflies, life span of females and males, effect of environmental factors on the activity of flies and their behaviour, potential fecundity of different species of botflies, duration of embryonal development, preservation of viability of larvae in egg membranes, localization of different stages of botflies in the host, and methods of their control.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Russian Summary Language Original Title Ekologicheskie osobennosti zheludochnykh ovodov loshadei v Uzbekistane
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0031-1847 ISBN Medium
Area Expedition Conference
Notes PMID:2958767 Approved no
Call Number Equine Behaviour @ team @ Serial 2680
Permanent link to this record