| Records |
| Author |
Crosby, M.B.; Svenson, J.L.; Zhang, J.; Nicol, C.J.; Gonzalez, F.J.; Gilkeson, G.S. |
| Title |
Peroxisome proliferation-activated receptor (PPAR)gamma is not necessary for synthetic PPARgamma agonist inhibition of inducible nitric-oxide synthase and nitric oxide |
Type |
Journal Article |
| Year |
2005 |
Publication |
The Journal of pharmacology and experimental therapeutics |
Abbreviated Journal  |
J Pharmacol Exp Ther |
| Volume |
312 |
Issue |
1 |
Pages |
69-76 |
| Keywords |
Animals; Cell Line; Gene Expression/drug effects; Macrophages/drug effects/metabolism; Mice; Mice, Inbred C57BL; Nitric Oxide/*metabolism; Nitric Oxide Synthase/*metabolism; Nitric Oxide Synthase Type II; PPAR delta/metabolism; PPAR gamma/*agonists/deficiency; Thiazolidinediones/pharmacology |
| Abstract |
Peroxisome proliferation-activated receptor (PPAR)gamma agonists inhibit inducible nitric-oxide synthase (iNOS), tumor necrosis factor-alpha, and interleukin-6. Because of these effects, synthetic PPARgamma agonists, including thiazolidinediones, are being studied for their impact on inflammatory disease. The anti-inflammatory concentrations of synthetic PPARgamma agonists range from 10 to 50 microM, whereas their binding affinity for PPARgamma is in the nanomolar range. The specificity of synthetic PPARgamma agonists for PPARgamma at the concentrations necessary for anti-inflammatory effects is thus in question. We report that PPARgamma is not necessary for the inhibition of iNOS by synthetic PPARgamma agonists. RAW 264.7 macrophages possess little PPARgamma, yet lipopolysaccharide (LPS)/interferon (IFN)gamma-induced iNOS was inhibited by synthetic PPARgamma agonists at 20 microM. Endogenous PPARgamma was inhibited by the transfection of a dominant-negative PPARgamma construct into murine mesangial cells. In the transfected cells, synthetic PPARgamma agonists inhibited iNOS production at 10 microM, similar to nontransfected cells. Using cells from PPARgamma Cre/lox conditional knockout mice, baseline and LPS/IFNgamma-induced nitric oxide levels were higher in macrophages lacking PPARgamma versus controls. However, synthetic PPARgamma agonists inhibited iNOS at 10 microM in the PPARgamma-deficient cells, similar to macrophages from wild-type mice. These results indicate that PPARgamma is not necessary for inhibition of iNOS expression by synthetic PPARgamma agonists at concentrations over 10 microM. Intrinsic PPARgamma function, in the absence of synthetic agonists, however, may play a role in inflammatory modulation. |
| Address |
Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA |
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Place of Publication |
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Editor |
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| Language |
English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0022-3565 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:15356214 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
73 |
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| Author |
Gavrilova, O.; Haluzik, M.; Matsusue, K.; Cutson, J.J.; Johnson, L.; Dietz, K.R.; Nicol, C.J.; Vinson, C.; Gonzalez, F.J.; Reitman, M.L. |
| Title |
Liver peroxisome proliferator-activated receptor gamma contributes to hepatic steatosis, triglyceride clearance, and regulation of body fat mass |
Type |
Journal Article |
| Year |
2003 |
Publication |
The Journal of biological chemistry |
Abbreviated Journal |
J Biol Chem |
| Volume |
278 |
Issue |
36 |
Pages |
34268-34276 |
| Keywords |
Adipose Tissue/*metabolism; Animals; Blotting, Southern; Blotting, Western; Female; Hypoglycemia/genetics; Insulin Resistance/genetics; Lipid Metabolism; Liver/*metabolism; Liver Diseases/genetics/*metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Mice, Transgenic; RNA/metabolism; Receptors, Cytoplasmic and Nuclear/*genetics/*physiology; Recombination, Genetic; Thiazoles/pharmacology; *Thiazolidinediones; Time Factors; Transcription Factors/*genetics/*physiology; Triglycerides/*metabolism |
| Abstract |
Peroxisome proliferator-activated receptor gamma (PPAR gamma) is a nuclear receptor that mediates the antidiabetic effects of thiazolidinediones. PPAR gamma is present in adipose tissue and becomes elevated in fatty livers, but the roles of specific tissues in thiazolidinedione actions are unclear. We studied the function of liver PPAR gamma in both lipoatrophic A-ZIP/F-1 (AZIP) and wild type mice. In AZIP mice, ablation of liver PPAR gamma reduced the hepatic steatosis but worsened the hyperlipidemia, triglyceride clearance, and muscle insulin resistance. Inactivation of AZIP liver PPAR gamma also abolished the hypoglycemic and hypolipidemic effects of rosiglitazone, demonstrating that, in the absence of adipose tissue, the liver is a primary and major site of thiazolidinedione action. In contrast, rosiglitazone remained effective in non-lipoatrophic mice lacking liver PPAR gamma, suggesting that adipose tissue is the major site of thiazolidinedione action in typical mice with adipose tissue. Interestingly, mice without liver PPAR gamma, but with adipose tissue, developed relative fat intolerance, increased adiposity, hyperlipidemia, and insulin resistance. Thus, liver PPAR gamma regulates triglyceride homeostasis, contributing to hepatic steatosis, but protecting other tissues from triglyceride accumulation and insulin resistance. |
| Address |
Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA. oksanag@bdg10.niddk.nih.gov |
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English |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0021-9258 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:12805374 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
81 |
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| Author |
Dixon, G.; Green, L.E.; Nicol, C.J. |
| Title |
Effect of diet change on the behavior of chicks of an egg-laying strain |
Type |
Journal Article |
| Year |
2006 |
Publication |
Journal of applied animal welfare science : JAAWS |
Abbreviated Journal |
J Appl Anim Welf Sci |
| Volume |
9 |
Issue |
1 |
Pages |
41-58 |
| Keywords |
*Animal Feed; *Animal Nutrition Physiology; Animals; Behavior, Animal/*physiology; Chickens/*physiology; Crowding; Feeding Behavior/*physiology; Female; Food Preferences/physiology; Oviposition; Random Allocation; Taste |
| Abstract |
Injurious pecking has serious welfare consequences in flocks of hens kept for egg laying, especially when loose-housed. Frequent diet change is a significant risk for injurious pecking; how the mechanics of diet change influence pecking behavior is unknown. This study investigated the effect of diet change on the behavior of chicks from a laying strain. The study included a 3-week familiarity phase: 18 chick pairs received unflavored feed (Experiment 1); 18 pairs received orange oil-flavored (Experiment 2). All chicks participated in a dietary preference test (P); a diet change (DC); or a control group (C), 6 scenarios. All P chicks preferred unflavored feed. In Experiment 1, DC involved change from unflavored to orange-flavored; Experiment 2, orange- flavored to unflavored. Compared with controls, Experiment 2 DC chicks exhibited few behavioral differences; Experiment 1 DC chicks exhibited increased behavioral event rates on Days 1 and 7. They pecked significantly longer at their environment; by Day 7, they showed significantly more beak activity. There was little evidence of dietary neophobia. Change from more preferred to less preferred feed led to increased activity and redirected pecking behavior. |
| Address |
School of Veterinary Science, University of Bristol, England |
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| Language |
English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
1088-8705 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:16649950 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
64 |
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| Author |
McGreevy, P.D.; Nicol, C.J. |
| Title |
Prevention of crib-biting: a review |
Type |
Journal Article |
| Year |
1998 |
Publication |
Equine veterinary journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
| Volume |
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Issue |
27 |
Pages |
35-38 |
| Keywords |
Animals; *Behavior, Animal; Horse Diseases/*prevention & control/psychology; Horses; *Stereotyped Behavior |
| Abstract |
Crib-biting is a common oral stereotype. Because of perceived deleterious effects on the health and appearance of subjects the prevention of crib-biting is regularly attempted. The resourcefulness of horses in satisfying their motivation to perform this behaviour often frustrates owners' efforts at prevention. This paper reviews the efficacy and observable consequences of attempting to prevent crib-biting by a variety of methods. These include attempts to prevent the grasping of objects, to interfere with air-engulfing and to introduce punishment for grasping and neck-flexion. Other approaches include the use of surgery, acupuncture, pharmaceuticals, operant feeding and environmental enrichment. A remedy that is effective for every crib-biter remains elusive. We conclude that, rather than concentrating on remedial prevention, further research should be directed at establishing why horses crib-bite and how the emergence of crib-biting can be avoided. |
| Address |
Department of Clinical Veterinary Science, University of Bristol, UK |
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Place of Publication |
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| Language |
English |
Summary Language |
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Original Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:10485002 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
87 |
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| Author |
McGreevy, P.D.; Nicol, C.J. |
| Title |
The effect of short-term prevention on the subsequent rate of crib-biting in thoroughbred horses |
Type |
Journal Article |
| Year |
1998 |
Publication |
Equine veterinary journal. Supplement |
Abbreviated Journal |
Equine Vet J Suppl |
| Volume |
|
Issue |
27 |
Pages |
30-34 |
| Keywords |
Analysis of Variance; Animals; *Behavior, Animal; Horse Diseases/*prevention & control/psychology; Horses; Male; Recurrence; *Stereotyped Behavior; Videotape Recording |
| Abstract |
The results of an experimental study of the motivational consequences of short-term prevention of crib-biting are reported here. Eight test horses wore a cribbing collar for 24 h. This was effective in preventing crib-biting in 6 subjects. Using analysis of co-variance that accounted for baseline differences in crib-biting rate, test horses showed significantly more crib-biting than control horses on the first day after prevention (P < 0.05). There was also a highly significant increase in the crib-biting rate of test horses on the first day after prevention in comparison with their baseline rate (P < 0.01). This defines the increase as a post inhibitory rebound. An increase in the novelty of the cribbing bar and an increase in feeding motivation during the period of prevention are rejected as explanations of the rebound in this study. Instead, it is suggested that the rebound reflected a rise in internal motivation to crib-bite during the period of prevention. Behaviours that exhibit this pattern of motivation are generally considered functional; and it has been argued that their prevention may compromise welfare. |
| Address |
Department of Clinical Veterinary Science, University of Bristol, UK |
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English |
Summary Language |
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Edition |
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ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:10485001 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
88 |
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| Author |
Waters, A.J.; Nicol, C.J.; French, N.P. |
| Title |
Factors influencing the development of stereotypic and redirected behaviours in young horses: findings of a four year prospective epidemiological study |
Type |
Journal Article |
| Year |
2002 |
Publication |
Equine veterinary journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
34 |
Issue |
6 |
Pages |
572-579 |
| Keywords |
Age Factors; Animal Husbandry/*methods; Animal Welfare; Animals; *Behavior, Animal; Female; Horse Diseases/epidemiology/prevention & control/*psychology; Horses; Housing, Animal; Longitudinal Studies; Male; Prevalence; Prospective Studies; Risk Factors; *Stereotyped Behavior; Weaning |
| Abstract |
Stereotypies are invariant and repetitive behaviour patterns that seemingly have no function, which tend to develop in captive animals faced with insoluble problems and may be indicative of reduced welfare. A 4 year prospective study of the factors influencing the development of stereotypic and redirected behaviours (abnormal behaviour) in a population of 225 young Thoroughbred and part-Thoroughbred horses was conducted between 1995 and 1999. Abnormal behaviour affected 34.7% of the population. Multivariable analysis showed that foals of low- or middle-ranking mares were less likely to develop abnormal behaviour than foals of dominant mares (rate ratio (RR) 0.23, P<0.01; RR 0.48, P<0.01, respectively). Weaning by confinement in a stable or barn was associated with an increased rate of development of abnormal behaviour, compared with paddock-weaning (RR 2.19, P<0.05), and housing in barns, rather than at grass after weaning, was associated with a further increase (RR 2.54, P<0.01). Specific stereotypic and redirected behaviours were then considered as separate outcomes. Crib-biting was initiated by 10.5% of horses at median age 20 weeks, weaving by 4.6% of horses at median age 60 weeks, box-walking by 2.3% of horses at median age 64 weeks and wood-chewing by 30.3% of horses at median age 30 weeks. Wood-chewing developed at a lower rate in horses born to subordinate or mid-ranking mares than in horses born to dominant mares (RR 0.29, P<0.01; RR 0.41, P<0.01, respectively), and at a higher rate in horses kept in barns or stables rather than at grass after weaning (RR 4.49, P<0.001; RR 1A6, P<0.001, respectively). Feeding concentrates after weaning was associated with a 4-fold increase in the rate of development of crib-biting (RR 4.12, P = 0.02). The results of this study support the idea that simple changes in feeding, housing and weaning practices could substantially lower the incidence of abnormal behaviour in young horses. |
| Address |
University of Bristol, Department of Clinical Veterinary Science, Langford, Bristol, UK |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:12357996 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
84 |
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| Author |
McGreevy, P.D.; Richardson, J.D.; Nicol, C.J.; Lane, J.G. |
| Title |
Radiographic and endoscopic study of horses performing an oral based stereotypy |
Type |
Journal Article |
| Year |
1995 |
Publication |
Equine veterinary journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
27 |
Issue |
2 |
Pages |
92-95 |
| Keywords |
Animals; Endoscopy/*veterinary; Esophagus/physiopathology/radiography; Female; Fluoroscopy/veterinary; Horse Diseases/physiopathology/*psychology/radiography; Horses; Male; Pharynx/physiopathology/radiography; *Stereotyped Behavior; Video Recording |
| Abstract |
There is confusion in the veterinary literature concerning the definition of oral based stereotypies in the horse. This study reports the use of fluoroscopy and endoscopy during cribbiting/wind-sucking in afflicted horses. This permitted observations of movements of the pharyngeal and oesophageal tissues and of the air column within during the stereotypic behaviour. The findings reported show that the sequence of events during crib-biting/wind-sucking is not related to deglutition and that air is not swallowed to the stomach. Transient dilation of the upper oesophagus was recorded and the characteristic noise of wind-sucking coincided with the in-rush of air through the cricopharynx. The oesophageal distension was relieved when the air returned to the pharynx although small quantities passed caudally. It is proposed that the role of contraction of the strap muscles of the neck is to create a pressure gradient in the soft tissues surrounding the oesophagus which provokes movement of air from the pharynx into the oesophagus. The findings suggest that the definitions currently used in the sale of horses are in need of revision. |
| Address |
Department of Clinical Veterinary Science, University of Bristol, Langford, UK |
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English |
Summary Language |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
0425-1644 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:7607156 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
90 |
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| Author |
McGreevy, P.D.; Cripps, P.J.; French, N.P.; Green, L.E.; Nicol, C.J. |
| Title |
Management factors associated with stereotypic and redirected behaviour in the thoroughbred horse |
Type |
Journal Article |
| Year |
1995 |
Publication |
Equine veterinary journal |
Abbreviated Journal |
Equine Vet J |
| Volume |
27 |
Issue |
2 |
Pages |
86-91 |
| Keywords |
Animal Husbandry/*methods; Animals; Horse Diseases/psychology/*therapy; Horses; Risk Factors; *Stereotyped Behavior; Time Factors |
| Abstract |
A greater knowledge of the effect of management factors is required to investigate the ontogeny of abnormal behaviour in the stabled horse. A postal survey of racehorse (flat) trainers yielded information about 22 yard and management factors. The relationship of the factors to the prevalence of abnormal behaviour was analysed by logistic regression. Management factors related to the time spent in the stable showed the strongest associations with stereotypic behaviour. The risk of horses performing abnormal behaviour increased: 1) as the amount of forage fell below 6.8 kg/day, 2) when bedding types other than straw were used, 3) when the total number of horses on the yard was fewer than 75, 4) in association with box designs that minimised contact between neighbouring horses, 5) when hay, rather than other types of forage, was used. |
| Address |
Department of Animal Health and Husbandry, School of Veterinary Science, University of Bristol, Langford, UK |
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English |
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Edition |
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0425-1644 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:7607155 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
91 |
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| Author |
Hothersall, B.; Nicol, C. |
| Title |
Role of Diet and Feeding in Normal and Stereotypic Behaviors in Horses |
Type |
Journal Article |
| Year |
2009 |
Publication |
Veterinary Clinics of North America: Equine Practice |
Abbreviated Journal |
Clinical Nutrition |
| Volume |
25 |
Issue |
1 |
Pages |
167-181 |
| Keywords |
Equine behavior; Diet; Crib-biting; Stereotypy; Weaning; Tryptophan; Insulin |
| Abstract |
This article reviews the effects of diet on equine feeding behavior and feeding patterns, before considering the evidence that diet affects reactivity in horses. A growing body of work suggests that fat- and fiber-based diets may result in calmer patterns of behavior, and possible mechanisms that may underpin these effects are discussed. In contrast, there is little evidence that herbal- or tryptophan-containing supplements influence equine behavior in any measurable way. The role of diet in the development of abnormal oral behaviors, particularly the oral stereotypy crib-biting, is also reviewed, and suggestions for future work are presented. |
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Edition |
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0749-0739 |
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Approved |
no |
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Equine Behaviour @ team @ |
Serial |
4945 |
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| Author |
Crosby, M.B.; Zhang, J.; Nowling, T.M.; Svenson, J.L.; Nicol, C.J.; Gonzalez, F.J.; Gilkeson, G.S. |
| Title |
Inflammatory modulation of PPAR gamma expression and activity |
Type |
Journal Article |
| Year |
2006 |
Publication |
Clinical immunology |
Abbreviated Journal |
Clin Immunol |
| Volume |
118 |
Issue |
2-3 |
Pages |
276-283 |
| Keywords |
Age Factors; Animals; Cell Line, Transformed; Cells, Cultured; Female; Inflammation Mediators/*physiology; Kidney/metabolism; Mesangial Cells/metabolism; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Inbred MRL lpr; Mice, Knockout; Nitric Oxide/biosynthesis; Nitric Oxide Synthase Type II/biosynthesis/genetics; PPAR gamma/*biosynthesis/*genetics/metabolism; Up-Regulation/immunology |
| Abstract |
Nitric oxide (NO) production increases with age in the lupus-prone MRL/lpr mouse, paralleling disease activity. One mechanism for excess NO production in MRL/lpr mice may be a defect in down-regulatory mechanisms of the iNOS pathway. A potential modulator of NO is the nuclear hormone receptor peroxisome proliferation activated receptor gamma (PPARgamma). We demonstrate that renal PPARgamma protein expression was altered as disease progressed in MRL/lpr mice, which paralleled increased iNOS protein expression. Additionally, MRL/lpr-derived primary mesangial cells expressed less PPARgamma than BALB/c mesangial cells and produced more NO in response to LPS and IFNgamma. Furthermore, PPARgamma activity was reduced in mesangial cells following exposure to inflammatory mediators. This activity was restored with the addition of a NOS enzyme inhibitor. These results indicate that the activation of inflammatory pathways may lead to reduced activity and expression of PPARgamma, further exacerbating the disease state. |
| Address |
Department of Medicine, Medical University of South Carolina, Charleston, SC 29425, USA |
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Place of Publication |
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English |
Summary Language |
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Original Title |
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Series Title |
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Abbreviated Series Title |
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Series Issue |
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Edition |
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| ISSN |
1521-6616 |
ISBN |
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Medium |
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Expedition |
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Conference |
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| Notes |
PMID:16303334 |
Approved |
no |
| Call Number |
refbase @ user @ |
Serial |
67 |
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