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	Author	Flauger, B.; Krueger, K.; Gerhards, H.; Möstl, E.
	Title	Simplified method to measure glucocorticoid metabolites in faeces of horses			Type	Journal Article
	Year	2010	Publication	Veterinary Research Communications	Abbreviated Journal	Vet Res Comm
	Volume	34	Issue	2	Pages	185-195
	Keywords	ACTH challenge; enzyme immunoassay; stress behaviour; cortisol
	Abstract	Glucocorticoids or their metabolites can be measured in several body fluids or excreta, including plasma, saliva, urine and faeces. In recent years the measurement of glucocorticoid metabolites (GCMs) in faeces has gained increasing attention, because of its suitability for wild populations. In horses, however, the group-specific enzyme immunoassay described so far has a limited racticability due to its complex extraction procedure. Therefore, we tested the applicability of other enzyme immunoassays for glucocorticoid metabolites. The present study clearly proved that an enzyme immunoassay (EIA) for 11-oxoetiocholanolone using 11-oxoetiocholanolone-17-CMO: BSA (3α,11-oxo-A EIA) as antigen showed high amounts of immunoreactive substances. Therefore it was possible to use just a small amount of the supernatant of a methanolic suspension of faeces. The results correlated well with the already described method for measuring GCMs in horse faeces, i.e. analysing the samples with an EIA after a two step clean up procedure of the samples (Merl et al. 2000). In addition, the 3α,11-oxo-A EIA has the advantage of providing a bigger difference between baseline values and peak values after ACTH stimulation. The new assay increased the accuracy of the test, lowered the expenses per sample, and storing samples at room temperature after collection was less critical than with other assays investigated in our study. This is a big advantage both in the field of wildlife management of equids and in the field of equestrian sports and it shows the importance of choosing an assay which is in good accordance with the metabolites excreted in a given species.
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	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5073
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	Author	Bates, L.A.; Lee, P.C.; Njiraini, N.; Poole, J.H.; Sayialel, K.; Sayialel, S.; Moss, C.J.; Byrne, R.
	Title	Do Elephants Show Empathy?			Type	Journal Article
	Year	2008	Publication	Journal of Consciousness Studies	Abbreviated Journal	J Conscious Stud
	Volume	15	Issue	10-11	Pages	204-225
	Keywords
	Abstract	Elephants show a rich social organization and display a number of unusual traits. In this paper, we analyse reports collected over a thirty-five year period, describing behaviour that has the potential to reveal signs of empathic understanding. These include coalition formation, the offering of protection and comfort to others, retrieving and 'babysitting' calves, aiding individuals that would otherwise have difficulty in moving, and removing foreign objects attached to others. These records demonstrate that an elephant is capable of diagnosing animacy and goal directedness, and is able to understand the physical competence, emotional state and intentions of others, when they differ from its own. We argue that an empathic understanding of others is the simplest explanation of these abilities, and discuss reasons why elephants appear to show empathy more than other non-primate species.
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	Notes				Approved	yes
	Call Number	Equine Behaviour @ team @			Serial	5057
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	Author	Hama, H.; Yogo, M.; Matsuyama, Y.
	Title	Effects of stroking horses on both humans' and horses' heart rate responses			Type	Journal Article
	Year	1996	Publication	Japanese Psychological Research	Abbreviated Journal	Jpn. Psychol. Res.
	Volume	38	Issue	2	Pages	66-73
	Keywords
	Abstract	The present study examined both human and horse heart rates (HRs) when humans stroked horses for 90 seconds; the subjective arousal levels of the humans were measured by the Tohoku Activation Deactivation Adjective Check List before and after stroking horses. Six male subjects with a positive attitude toward companion animals and 6 male subjects with a negative attitude were selected by their scores on the Pet Attitude Scale, and these two groups, together with a third group, of 6 subjects who were male members of the Doshisha University horse-riding club, participated in this experiment. The HRs of the human subjects during the first 10 seconds immediately after the stroking began were significantly higher than those obtained after that period, but these higher levels gradually returned to baseline levels. This tendency appears more clearly in the negative attitude group. The HRs of the horses increased during the first 20 seconds immediately after the human subjects of the NA group started stroking them, but gradually reduced as the stroking continued. The results of subjective arousal levels suggest a decrease in tension by stroking horses. These results suggest that a certain affectional interaction may exist between humans and companion animals.
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	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5056
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	Author	Broom, D.M.; Sena, H.; Moynihan, K.L.
	Title	Pigs learn what a mirror image represents and use it to obtain information			Type	Journal Article
	Year	2009	Publication	Animal Behaviour.	Abbreviated Journal	Anim. Behav.
	Volume	78	Issue	5	Pages	1037-1041
	Keywords	awareness; cognition; learning; mirror; pig; Sus scrofa
	Abstract	Mirror usage has been taken to indicate some degree of awareness in animals. Can pigs, Sus scrofa, obtain information from a mirror? When put in a pen with a mirror in it, young pigs made movements while apparently looking at their image. After 5 h spent with a mirror, the pigs were shown a familiar food bowl, visible in the mirror but hidden behind a solid barrier. Seven out of eight pigs found the food bowl in a mean of 23 s by going away from the mirror and around the barrier. Naïve pigs shown the same looked behind the mirror. The pigs were not locating the food bowl by odour, did not have a preference for the area where the food bowl was and did not go to that area when the food bowl was visible elsewhere. To use information from a mirror and find a food bowl, each pig must have observed features of its surroundings, remembered these and its own actions, deduced relationships among observed and remembered features and acted accordingly. This ability indicates assessment awareness in pigs. The results may have some effects on the design of housing conditions for pigs and may lead to better pig welfare.
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	Series Volume		Series Issue		Edition
	ISSN	0003-3472	ISBN		Medium
	Area		Expedition		Conference
	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5053
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	Author	Ishida, N.; Oyunsuren, T.; Mashima, S.; Mukoyama, H.; Saitou, N.
	Title	Mitochondrial DNA sequences of various species of the genus Equus with special reference to the phylogenetic relationship between Przewalskii's wild horse and domestic horse			Type	Journal Article
	Year	1995	Publication	Journal of Molecular Evolution	Abbreviated Journal	J Mol Evol
	Volume	41	Issue	2	Pages	180-188
	Keywords	Animals; Base Sequence; Chromosomes; Conserved Sequence/genetics; DNA, Mitochondrial/genetics; Evolution; Genetic Variation/genetics; Horses/genetics; Molecular Sequence Data; Phylogeny; RNA, Transfer, Pro/genetics; Sequence Alignment; Sequence Analysis, DNA
	Abstract	The noncoding region between tRNAPro and the large conserved sequence block is the most variable region in the mammalian mitochondrial DNA D-loop region. This variable region (ca. 270 bp) of four species of Equus, including Mongolian and Japanese native domestic horses as well as Przewalskii's (or Mongolian) wild horse, were sequenced. These data were compared with our recently published Thoroughbred horse mitochondrial DNA sequences. The evolutionary rate of this region among the four species of Equus was estimated to be 2-4 x 10(-8) per site per year. Phylogenetic trees of Equus species demonstrate that Przewalskii's wild horse is within the genetic variation among the domestic horse. This suggests that the chromosome number change (probably increase) of the Przewalskii's wild horse occurred rather recently.
	Address	Laboratory of Molecular and Cellular Biology, Japan Racing Association, Tokyo
	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN	0022-2844	ISBN		Medium
	Area		Expedition		Conference
	Notes	PMID:7666447			Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5042
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	Author	Oakenfull, E.A.; Lim, H.; Ryder, O.
	Title	A survey of equid mitochondrial DNA: Implications for the evolution, genetic diversity and conservation of Equus			Type	Journal Article
	Year	2000	Publication	Conservation Genetics	Abbreviated Journal	Conservat Genet
	Volume	1	Issue	4	Pages	341-355
	Keywords
	Abstract	The evolution, taxonomy and conservation of the genus Equuswere investigated by examining the mitochondrial DNA sequences of thecontrol region and 12S rRNA gene. The phylogenetic analysis of thesesequences provides further evidence that the deepest node in thephylogeny of the extant species is a divergence between twolineages; one leading to the ancestor of modern horses (E.ferus, domestic and przewalskii) and the other to thezebra and ass ancestor, with the later speciation events of the zebrasand asses occurring either as one or more rapid radiations, or withextensive secondary contact after speciation. Examination of the geneticdiversity within species suggested that two of the E. hemionussubspecies (E. h. onager and E. h. kulan) onlyrecently diverged, and perhaps, are insufficiently different to beclassified as separate subspecies. The genetic divergence betweendomestic and wild forms of E. ferus (horse) and E.africanus (African ass) was no greater than expected within anequid species. In E. burchelli (plains zebra) there was anindication of mtDNA divergence between populations increasing withdistance. The implications of these results for equid conservation arediscussed and recommendations are made for conservation action.
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	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5039
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	Author	Whitehead, H.
	Title	SOCPROG programs: analysing animal social structures			Type	Journal Article
	Year	2009	Publication	Behavioral Ecology and Sociobiology	Abbreviated Journal	Behav. Ecol. Sociobiol.
	Volume	63	Issue	5	Pages	765-778
	Keywords	Social analysis – Software – Association
	Abstract	Abstract SOCPROG is a set of programs which analyses data on animal associations. Data usually come from observations of the social behaviour of individually identifiable animals. Associations among animals, sampling periods, restrictions on the data and association indices can be defined very flexibly. SOCPROG can analyse data sets including 1,000 or more individuals. Association matrices are displayed using sociograms, principal coordinates analysis, multidimensional scaling and cluster analyses. Permutation tests, Mantel and related tests and matrix correlation methods examine hypotheses about preferred associations among individuals and classes of individual. Weighted network statistics are calculated and can be tested against null hypotheses. Temporal analyses include displays of lagged association rates (rates of reassociation following an association). Models can be fitted to lagged association rates. Multiple association measures, including measures produced by other programs such as genetic or range use data, may be analysed using Mantel tests and principal components analysis. SOCPROG also performs mark-recapture population analyses and movement analyses. SOCPROG is written in the programming language MATLAB and may be downloaded free from the World Wide Web.
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	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5026
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	Author	Davis, M. H.
	Title	Empathy: A Social Psychological Approach			Type	Book Whole
	Year	1996	Publication		Abbreviated Journal
	Volume		Issue		Pages	272
	Keywords
	Abstract	Product Description Empathy has long been a topic of interest to psychologists, but it has been studied in a sometimes bewildering number of ways. In this volume, Mark Davis offers a thorough, evenhanded review of contemporary empathy research, especially work that has been carried out by social and personality psychologists.Davis’ approach is explicitly multidimensional. He draws careful distinctions between situational and dispositional “antecedents” of empathy, cognitive and noncognitive “internal processes,” affective and nonaffective “intrapersonal outcomes,” and the “interpersonal behaviora l outcomes” that follow. Davis presents a novel organizational model to help classify and interpret previous findings. This book will be of value in advanced undergraduate and graduate courses on altruism, helping, nad moral development. About the Author Mark H. Davis is associate professor of psychology at Eckerd College in St. Petersburg, Florida.
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	Corporate Author				Thesis
	Publisher	Westview Press	Place of Publication	Boulder, CO	Editor
	Language		Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
	ISSN		ISBN	978-0813330013	Medium
	Area		Expedition		Conference
	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5017
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	Author	Albright, J.D.; Mohammed, H.O.; Heleski, C.R.; Wickens, C.L.; Houpt, K.A.
	Title	Crib-biting in US horses: Breed predispositions and owner perceptions of aetiology			Type	Journal Article
	Year	2009	Publication	Equine Veterinary Journal	Abbreviated Journal
	Volume	41	Issue	5	Pages	455-458
	Keywords	HORSE; BEHAVIOUR; CRIB-BITING; BREED PREVALENCE; LEARNING
	Abstract	Reasons for performing study: Crib-biting is an equine stereotypy that may result in diseases such as colic. Certain breeds and management factors have been associated. Objectives: To determine: breed prevalence of crib-biting in US horses; the likelihood that one horse learns to crib-bite from another; and owner perceptions of causal factors. Methods: An initial postal survey queried the number and breed of crib-biting horses and if a horse began after being exposed to a horse with this habit. In a follow-up survey, a volunteer subset of owners was asked the number of affected and nonaffected horses of each breed and the extent of conspecific contact. The likelihood of crib-biting given breed and extent of contact was quantified using odds ratio (OR) and significance of the association was assessed using the Chi-squared test. Results: Overall prevalence was 4.4%. Thoroughbreds were the breed most affected (13.3%). Approximately half of owners believed environmental factors predominantly cause the condition (54.4%) and crib-biting is learned by observation (48.8%). However, only 1.0% of horses became affected after being exposed to a crib-biter. The majority (86%) of horses was turned out in the same pasture with other horses and extent of contact with conspecifics was not statistically related to risk. Conclusion: This is the first study to report breed prevalence for crib-biting in US horses. Thoroughbreds were the breed more likely to be affected. More owners believed either environmental conditions were a predominant cause or a combination of genetic and environmental factors contributes to the behaviour. Only a small number of horses reportedly began to crib-bite after being exposed to an affected individual, but approximately half of owners considered it to be a learned behaviour; most owners did not isolate affected horses. Potential relevance: Genetic predisposition, not just intensive management conditions and surroundings, may be a factor in the high crib-biting prevalence in some breeds, and warrants further investigation. Little evidence exists to suggest horses learn the behaviour from other horses, and isolation may cause unnecessary stress.
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	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	5010
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	Author	Lau, A.N.; Peng, L.; Goto, H.; Chemnick, L.; Ryder, O.A.; Makova, K.D.
	Title	Horse Domestication and Conservation Genetics of Przewalski's Horse Inferred from Sex Chromosomal and Autosomal Sequences			Type	Journal Article
	Year	2009	Publication	Mol Biol Evol	Abbreviated Journal	Mol Biol Evol
	Volume	26	Issue	1	Pages	199-208
	Keywords
	Abstract	Despite their ability to interbreed and produce fertile offspring, there is continued disagreement about the genetic relationship of the domestic horse (Equus caballus) to its endangered wild relative, Przewalski's horse (Equus przewalskii). Analyses have differed as to whether or not Przewalski's horse is placed phylogenetically as a separate sister group to domestic horses. Because Przewalski's horse and domestic horse are so closely related, genetic data can also be used to infer domestication-specific differences between the two. To investigate the genetic relationship of Przewalski's horse to the domestic horse and to address whether evolution of the domestic horse is driven by males or females, five homologous introns (a total of [~]3 kb) were sequenced on the X and Y chromosomes in two Przewalski's horses and three breeds of domestic horses: Arabian horse, Mongolian domestic horse, and Dartmoor pony. Five autosomal introns (a total of [~]6 kb) were sequenced for these horses as well. The sequences of sex chromosomal and autosomal introns were used to determine nucleotide diversity and the forces driving evolution in these species. As a result, X chromosomal and autosomal data do not place Przewalski's horses in a separate clade within phylogenetic trees for horses, suggesting a close relationship between domestic and Przewalski's horses. It was also found that there was a lack of nucleotide diversity on the Y chromosome and higher nucleotide diversity than expected on the X chromosome in domestic horses as compared with the Y chromosome and autosomes. This supports the hypothesis that very few male horses along with numerous female horses founded the various domestic horse breeds. Patterns of nucleotide diversity among different types of chromosomes were distinct for Przewalski's in contrast to domestic horses, supporting unique evolutionary histories of the two species.
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	Corporate Author				Thesis
	Publisher		Place of Publication		Editor
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	Series Editor		Series Title		Abbreviated Series Title
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	ISSN		ISBN		Medium
	Area		Expedition		Conference
	Notes				Approved	no
	Call Number	Equine Behaviour @ team @			Serial	4997
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