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Author (up) Palme, R.;Touma, C.;Arias,N.;Dominchin, M.F.;Lepschy, M. openurl 
  Title Steroid extraction: Get the best out of faecal samples Type Journal Article
  Year 3012 Publication Veterinary Medicine Austria Abbreviated Journal Vet. Med. Austria  
  Volume 100 Issue Pages 238-246  
  Keywords  
  Abstract Faecal steroid hormone metabolites are becoming increasingly popular as parameters for reproductive functions and stress. Theextraction of the steroids from the faecal matrix represents the initial step before quantification can be performed. The steroid metabolites present in the faecal matrix are of varying polarity and composition, so selection of a proper extraction procedure is essential. There have been some studies to address this complex but often neglected point. Radiolabelled
steroids (e.g. cortisol or progesterone) have frequently been added to faecal samples to estimate the efficiency of the extraction procedures used. However, native, unmetabolized steroids are normally not present in the faeces and therefore the results are artificial and do not accurately reflect the actual recoveries of the substances of interest. In this respect, recovery experiments based on faecal samples from radiometabolism studies are more informative. In these samples, the metabolite content accurately reflects the mixture of metabolites present in the given species. As a result, it is possible to evaluate different extraction methods for use with faecal samples. We present studies on sheep, horses, pigs, hares and dogs that utilized samples containing naturally metabolized, 14C-labelled steroids.
 
  Address Review, faeces, extrac- tion, non-invasive hormone moni- toring, stress, reproduction.  
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  Notes Approved no  
  Call Number Equine Behaviour @ team @ Serial 6046  
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